FastQCFastQC Report
Thu 26 May 2016
SRR936140_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936140_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2612659
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54450.20840836863899956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA2103.6379788E-1223.66754759
CGGTTAC2600.023.2088474
TATCGCG800.002451941822.19554766
CGTAACG1801.4703801E-719.71955732
TAACGCG1351.2672324E-418.40844552
ATTCGCG2559.367795E-1018.09663456
AACGCGA1452.1905906E-417.13758553
GCGTATA2551.8308128E-816.70522543
TCGCGCT2551.8317223E-816.70458637
AATTCGC2803.248715E-916.48054955
TACGTAG2452.1153573E-715.9366232
TCGCGGA2956.4774213E-915.64405258
CAATTCG3008.09996E-915.38184454
CGATCGT3301.7553248E-915.0582831
TTAGTCG2151.7302902E-514.85959515
CTCGCGC2908.904681E-814.68795236
ATAACGC1707.343656E-414.6184751
ACGCGAA1707.352183E-414.61623254
ACGATCG2252.655228E-514.20052859
TTACGGC2252.6561864E-514.19998558
Produced by FastQC (version 0.11.5)