FastQCFastQC Report
Thu 26 May 2016
SRR936140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936140_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2612659
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79150.30294806938065777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57610.220503326304734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39250.1502300912595176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA11950.046.064552100-101
CGGTATC8150.044.906635100-101
CGTACAT14500.036.11667100-101
GGTATCA80800.035.2797471
ACGTATC4100.031.206684100-101
GTATCAA159750.030.6591631
ACGGTAT3500.029.75521100-101
TCGTATC5400.028.653166100-101
CGAGTAC10950.026.901974100-101
TACGCAG9750.026.855988100-101
CCGTATC5800.026.677088100-101
ACCGTAT4250.026.60364298-99
TCGTACA9250.026.377594100-101
GACGCAG8900.026.0776100-101
CCTGTCG6300.025.9752896-97
TCGTATA4400.025.697681100-101
CCGGTAT3950.025.612082100-101
GCGTACA5350.025.58392100-101
CCGTACA8100.025.34703100-101
TCCGGTA3300.025.2458898-99
Produced by FastQC (version 0.11.5)