FastQCFastQC Report
Thu 26 May 2016
SRR936139_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936139_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798258
Sequences flagged as poor quality0
Sequence length77
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293333.6746264992020126No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA166872.0904269045847332No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG11070.1386769691002157No Hit
GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC8880.11124222995572859No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCA556.0900056E-638.7137072
CGGTTAC1301.4061516E-724.5667764
GAGCGTC750.00168404523.67469247
CGTCAAC901.7563315E-423.6568934
TTCGCGA952.5287442E-422.4174125
TCGCGAT952.5287442E-422.4174126
AACGATC800.002445447522.20337568
CGATCCG800.00245079822.19502346
TACGTAG800.002460631822.1797282
GCGTCAA1003.582984E-421.2912043
CAAACGT1106.789984E-419.36534742
GATCCGA950.0066292118.69054647
CGATTAA950.006650832518.68000617
ATCCGAA950.006653238618.67883533
AGTCACG1551.7616978E-518.3150144
GACGAGT1752.437957E-618.25875742
TAGACGA1752.4438032E-618.25417940
GCCTAGT1752.4452675E-618.25303517
ACTATGT1000.00888488417.7649370
CTAGCCC1000.00891381917.75490648
Produced by FastQC (version 0.11.5)