##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936139_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 798258 Sequences flagged as poor quality 0 Sequence length 77 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.969325706726394 40.0 36.0 41.0 31.0 41.0 2 36.78533130892519 40.0 36.0 41.0 31.0 41.0 3 36.672169899957154 39.0 36.0 41.0 30.0 41.0 4 36.54878497929241 39.0 35.0 41.0 30.0 41.0 5 36.40009746222399 39.0 35.0 41.0 30.0 41.0 6 36.18268529723473 39.0 35.0 40.0 30.0 41.0 7 35.84272252830539 38.0 35.0 40.0 30.0 41.0 8 35.49807581007644 38.0 35.0 40.0 29.0 41.0 9 35.28644122576911 38.0 35.0 40.0 29.0 41.0 10-11 35.324997056089636 38.0 35.0 40.0 28.5 41.0 12-13 35.24661137126092 38.0 35.0 40.0 27.5 41.0 14-15 35.019449852052844 38.0 35.0 41.0 26.5 41.0 16-17 34.6568784778856 38.0 34.5 41.0 24.5 41.0 18-19 34.231758403924545 38.0 34.0 40.0 22.0 41.0 20-21 33.61205023939628 38.0 33.0 40.0 15.0 41.0 22-23 32.80612596428723 38.0 33.0 40.0 2.0 41.0 24-25 32.189013451791276 37.5 32.5 40.0 2.0 41.0 26-27 31.12665140844188 36.0 30.5 39.5 2.0 40.5 28-29 31.093241783984624 36.0 30.5 39.5 2.0 40.5 30-31 31.37382400176384 36.5 31.0 40.0 2.0 41.0 32-33 31.172991063039767 36.0 31.0 40.0 2.0 41.0 34-35 30.877732512546068 35.5 31.0 40.0 2.0 41.0 36-37 30.574283001235187 35.0 30.0 39.5 2.0 41.0 38-39 30.293651927071195 35.0 30.0 39.0 2.0 41.0 40-41 29.90625086125037 35.0 29.0 39.0 2.0 41.0 42-43 29.54977713972174 35.0 29.0 38.5 2.0 40.5 44-45 29.183237750201066 35.0 28.5 38.0 2.0 40.0 46-47 28.809048578279203 35.0 28.0 37.0 2.0 40.0 48-49 28.36460893595805 35.0 26.5 36.5 2.0 39.0 50-51 27.942780404330428 34.0 26.0 36.0 2.0 39.0 52-53 27.522430091524292 34.0 26.0 35.5 2.0 38.5 54-55 27.07513360342145 34.0 24.5 35.0 2.0 37.0 56-57 26.628765386629386 34.0 23.5 35.0 2.0 37.0 58-59 26.195249530853435 33.5 21.0 35.0 2.0 36.5 60-61 25.83240130383911 33.0 20.0 35.0 2.0 36.0 62-63 25.45413011833267 33.0 17.5 35.0 2.0 36.0 64-65 25.05521523116586 33.0 9.5 35.0 2.0 35.0 66-67 24.633993646164527 33.0 3.5 35.0 2.0 35.0 68-69 24.245369291632528 33.0 2.0 35.0 2.0 35.0 70-71 23.799093150334855 32.0 2.0 35.0 2.0 35.0 72-73 23.308653969017534 32.0 2.0 35.0 2.0 35.0 74-75 22.743064272453267 32.0 2.0 35.0 2.0 35.0 76-77 21.358853278012873 29.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6178442254502059 1101 2 0.6677335020543822 1101 3 0.6715853524187594 1101 4 0.7974583786911467 1101 5 0.9970994475925394 1101 6 0.8416084504558725 1101 7 1.01005507591465 1101 8 0.9755767339608639 1101 9 1.100840658837221 1101 10-11 0.9401833583867543 1101 12-13 1.0588077932378752 1101 14-15 1.2370191488458246 1101 16-17 1.328067161796021 1101 18-19 1.3410381460276355 1101 20-21 1.2749942873561864 1101 22-23 1.369667190437731 1101 24-25 1.3387399343949014 1101 26-27 1.0395031844255556 1101 28-29 0.7907634832300481 1101 30-31 1.0386725375346764 1101 32-33 1.0575328225182865 1101 34-35 1.023389269805545 1101 36-37 1.055432996377025 1101 38-39 0.9424689617429003 1101 40-41 1.005619788978862 1101 42-43 0.7948113162646031 1101 44-45 0.7649659475998902 1101 46-47 0.7460351711594129 1101 48-49 0.6385709763612653 1101 50-51 0.6398330227934288 1101 52-53 0.7808513909286354 1101 54-55 0.6706526584269525 1101 56-57 0.6723558474859246 1101 58-59 0.7170742313341414 1101 60-61 0.5871788532018201 1101 62-63 0.46102679261200663 1101 64-65 0.5065368700617157 1101 66-67 0.2310824861132268 1101 68-69 0.47854499180275667 1101 70-71 0.5890558005753519 1101 72-73 0.5150166644035075 1101 74-75 0.5482544655923967 1101 76-77 0.3944890824961007 1102 1 1.0865545323368266 1102 2 1.1832681595266052 1102 3 0.8945159012405384 1102 4 0.8591422281768644 1102 5 0.9964823887195067 1102 6 1.094658684657766 1102 7 1.1219310550949189 1102 8 1.0512145284161463 1102 9 1.204877185517006 1102 10-11 0.8937526833483886 1102 12-13 1.3150074318176834 1102 14-15 0.8984972452592643 1102 16-17 0.9015302244114665 1102 18-19 0.9744831376332357 1102 20-21 0.9370073016887872 1102 22-23 0.9548954421176106 1102 24-25 0.9072374761765971 1102 26-27 0.7555699550038675 1102 28-29 0.7886959353178362 1102 30-31 0.757222569389338 1102 32-33 0.6992329799885013 1102 34-35 0.5389880372905509 1102 36-37 0.5042100978832131 1102 38-39 0.7072142646827508 1102 40-41 0.6569795222812083 1102 42-43 0.6980242296239254 1102 44-45 0.827866885609513 1102 46-47 0.7048525016986176 1102 48-49 0.7410097613162456 1102 50-51 0.9182527911158687 1102 52-53 0.6975784946386021 1102 54-55 0.7055475380403706 1102 56-57 0.7022651718865944 1102 58-59 0.723475215422841 1102 60-61 0.7024827146280295 1102 62-63 0.685235534813625 1102 64-65 0.6852455341292547 1102 66-67 0.7891100614324777 1102 68-69 0.6838993799327859 1102 70-71 0.7241756662648768 1102 72-73 0.3595348576230748 1102 74-75 0.41613675159517527 1102 76-77 0.546710294095206 1103 1 0.6806139382774177 1103 2 0.7165071977161404 1103 3 0.43765592952907895 1103 4 0.5904010683465941 1103 5 0.7008671129062165 1103 6 0.391688387628065 1103 7 0.8744063026196756 1103 8 0.8984563954599949 1103 9 0.6716381473274708 1103 10-11 0.8414188785393293 1103 12-13 0.9769593412943394 1103 14-15 1.0505481646369148 1103 16-17 0.9128456416674808 1103 18-19 1.086930096614843 1103 20-21 1.059355250769407 1103 22-23 1.0574414110002195 1103 24-25 0.9539135723576422 1103 26-27 0.8192191770689305 1103 28-29 0.7377765576657822 1103 30-31 0.9078590616099937 1103 32-33 1.1454847480224437 1103 34-35 1.2207419269652284 1103 36-37 1.1081704939228167 1103 38-39 1.2206513226742608 1103 40-41 1.2277008801312803 1103 42-43 1.2828898590440083 1103 44-45 1.2670465178584536 1103 46-47 0.8024479755317167 1103 48-49 0.8216321092653267 1103 50-51 0.8546035690508056 1103 52-53 0.7294031056711354 1103 54-55 0.5485461236132139 1103 56-57 0.712166161985607 1103 58-59 0.6173931786477347 1103 60-61 0.6678292492814961 1103 62-63 0.6979653792266376 1103 64-65 0.652713709518224 1103 66-67 0.9652062424791517 1103 68-69 1.0035599174151066 1103 70-71 0.932803671922585 1103 72-73 1.0823071348507938 1103 74-75 0.9472541490492077 1103 76-77 1.2065405628363663 1104 1 0.4488355718537136 1104 2 0.17536013792514638 1104 3 0.4408021108659952 1104 4 0.13844612104416143 1104 5 0.21336960411683492 1104 6 0.0066739627929663925 1104 7 0.3416664740149642 1104 8 0.15327579962045945 1104 9 0.11704532240104726 1104 10-11 0.17730759737017365 1104 12-13 0.23914789159357497 1104 14-15 0.35620567977283457 1104 16-17 0.5386822530792514 1104 18-19 0.5505323001027378 1104 20-21 0.4426824246046337 1104 22-23 0.6513489256524068 1104 24-25 0.5339438345200733 1104 26-27 0.6872188752212764 1104 28-29 0.8235351530680219 1104 30-31 0.9516298741026183 1104 32-33 0.8346193933611268 1104 34-35 0.8889271840291464 1104 36-37 0.7573964288282049 1104 38-39 0.7022134616905866 1104 40-41 0.8008089628762356 1104 42-43 1.001771977859761 1104 44-45 0.7974886279283595 1104 46-47 0.6565636489712503 1104 48-49 0.6954868779745631 1104 50-51 0.6344560119851508 1104 52-53 0.553288542876178 1104 54-55 0.49865128656257696 1104 56-57 0.4926668034118755 1104 58-59 0.4409283951750531 1104 60-61 0.6323166790680546 1104 62-63 0.7043777069944959 1104 64-65 0.512933090846623 1104 66-67 0.7921459522797214 1104 68-69 0.3775036924844244 1104 70-71 0.5962290236428274 1104 72-73 0.5278197631118644 1104 74-75 0.5144916818703003 1104 76-77 0.395870635183055 1105 1 0.006900759506123677 1105 2 0.2322580740460083 1105 3 0.4247877172420118 1105 4 0.3848421390064587 1105 5 0.2551323026239416 1105 6 0.02665663988806699 1105 7 0.3808288781070388 1105 8 0.3862800225431968 1105 9 0.07042589100867502 1105 10-11 0.22578849756644814 1105 12-13 0.21437074741943007 1105 14-15 0.2254028783691595 1105 16-17 0.06944156135261181 1105 18-19 0.003902645966057605 1105 20-21 0.3107225944137113 1105 22-23 0.36856830202783186 1105 24-25 0.5477519740549539 1105 26-27 0.3326773745967593 1105 28-29 0.4451755875532406 1105 30-31 0.33819179791156984 1105 32-33 0.40054958874565116 1105 34-35 0.6998157077905205 1105 36-37 0.898588326466939 1105 38-39 0.7695720532566348 1105 40-41 0.5502392772163311 1105 42-43 0.36430301782883134 1105 44-45 0.29027694649137814 1105 46-47 0.29700742759493437 1105 48-49 0.42556367866450273 1105 50-51 0.5498646615420419 1105 52-53 0.3797398008313344 1105 54-55 0.5091314581559843 1105 56-57 0.7075145031085412 1105 58-59 0.44569498204235813 1105 60-61 0.26529692500869473 1105 62-63 0.3774227465472215 1105 64-65 0.520681931029813 1105 66-67 0.6125564976910063 1105 68-69 0.34208653016954926 1105 70-71 0.1806214952421854 1105 72-73 0.05133579237493535 1105 74-75 0.1410306695584005 1105 76-77 0.32059220480180173 1106 1 -0.0948778966203605 1106 2 0.17613740443428583 1106 3 -0.41838828833808606 1106 4 -0.11879800282102337 1106 5 -0.3977173253695483 1106 6 0.0034716247753081575 1106 7 0.1986183456727204 1106 8 0.22021399138765219 1106 9 0.15065432625628716 1106 10-11 0.03882979610167325 1106 12-13 0.09903783065588101 1106 14-15 0.1674076447025712 1106 16-17 -0.0056063926115754725 1106 18-19 0.07033705075564001 1106 20-21 -0.058809927092916325 1106 22-23 0.13138872169766813 1106 24-25 0.2457800489212687 1106 26-27 -0.04687825200170437 1106 28-29 0.040237055816437106 1106 30-31 -0.010626064213514752 1106 32-33 0.002244688722399246 1106 34-35 -0.06258693113066016 1106 36-37 0.060763547345803914 1106 38-39 0.03785268566770483 1106 40-41 0.0042506989787121086 1106 42-43 -0.08285679965547388 1106 44-45 -0.2333222164939741 1106 46-47 -0.0011281804032066134 1106 48-49 -0.0334647449579073 1106 50-51 -0.2680592224384277 1106 52-53 -0.04295259628561965 1106 54-55 0.19962131516598447 1106 56-57 0.25541397416178313 1106 58-59 0.18575180022417825 1106 60-61 0.3015275981079739 1106 62-63 0.036767956963405624 1106 64-65 -0.3904215914068274 1106 66-67 -0.12911695034432924 1106 68-69 -0.3372394986609528 1106 70-71 -0.45138632270712975 1106 72-73 -0.6004603015567085 1106 74-75 -0.8115094373934717 1106 76-77 -0.8694726057073794 1107 1 -0.03280235748059823 1107 2 -0.17822827845271405 1107 3 -0.042860011973324674 1107 4 -0.40071010043738653 1107 5 -0.0997116343525235 1107 6 -0.2689139766712785 1107 7 -0.6144664378653815 1107 8 -0.5052522584984516 1107 9 -0.1841292118461908 1107 10-11 -0.19626457525820484 1107 12-13 -0.3486768265164173 1107 14-15 -0.6661187183030179 1107 16-17 -0.4969813829747025 1107 18-19 -0.43327415185718365 1107 20-21 -0.5894061522385172 1107 22-23 -0.4820846910202121 1107 24-25 -0.7459488778454393 1107 26-27 -0.8153136077953995 1107 28-29 -0.5530374516512708 1107 30-31 -0.3704392138269874 1107 32-33 -0.20540997447732323 1107 34-35 -0.3617814629884428 1107 36-37 -0.42324536063773976 1107 38-39 -0.4612388191577459 1107 40-41 -0.2969412957507913 1107 42-43 -0.2136065140533283 1107 44-45 -0.20509316006739908 1107 46-47 -0.12043979161983742 1107 48-49 0.046409239665131 1107 50-51 0.03896571430858131 1107 52-53 0.14544582396655414 1107 54-55 0.09493673908367484 1107 56-57 -0.042826506350689186 1107 58-59 -0.03200406776104714 1107 60-61 -0.08630697692304068 1107 62-63 0.15354068774044904 1107 64-65 0.1799057472016763 1107 66-67 0.07964507823690781 1107 68-69 0.08021797640580175 1107 70-71 -0.06665131944811264 1107 72-73 -0.15439530341399887 1107 74-75 -0.19186579177842944 1107 76-77 -0.3946818136607888 1108 1 0.06038125863879884 1108 2 0.19320737738661364 1108 3 -0.012526926594368604 1108 4 0.1428176999711468 1108 5 0.06148232500657258 1108 6 -0.03823375516536487 1108 7 -0.14974089974535332 1108 8 -0.0697381073879697 1108 9 0.05878342556186311 1108 10-11 -0.16880067623467454 1108 12-13 -0.0808920036857188 1108 14-15 -0.06386115274269599 1108 16-17 -0.1646930002276008 1108 18-19 -0.34874532420908366 1108 20-21 -0.5949319926267478 1108 22-23 -0.15195114987749037 1108 24-25 -0.27854307099008935 1108 26-27 -0.30231961773719007 1108 28-29 -0.10471232442705869 1108 30-31 -0.02045751522143746 1108 32-33 0.010457924876813962 1108 34-35 -0.17028984030416083 1108 36-37 -0.1636607432549546 1108 38-39 -0.26741524460022603 1108 40-41 -0.2784155616287265 1108 42-43 -0.08475288978685924 1108 44-45 -0.1495277650524649 1108 46-47 -0.03742230493262255 1108 48-49 -0.05947318273105395 1108 50-51 -0.07144482729461998 1108 52-53 -0.25653544957435415 1108 54-55 -0.17411147033151053 1108 56-57 -0.11740173688922084 1108 58-59 -0.331432591960219 1108 60-61 -0.5191172445956909 1108 62-63 -0.7003551478067607 1108 64-65 -0.4096293973793408 1108 66-67 -0.5329970527538457 1108 68-69 -0.4131226774853083 1108 70-71 -0.40114767688024955 1108 72-73 -0.4762577950012634 1108 74-75 -0.6260220974943671 1108 76-77 -0.3956677274907392 1109 1 0.4511593994169374 1109 2 0.650736334915571 1109 3 0.20271413864669796 1109 4 0.28101688817033477 1109 5 0.3403260038502509 1109 6 0.33273941580780075 1109 7 0.39355184354739237 1109 8 0.4822666446167787 1109 9 0.6110752778537147 1109 10-11 0.1771576447236427 1109 12-13 0.45477278197906656 1109 14-15 0.6834435695620229 1109 16-17 0.7376062770716771 1109 18-19 0.6924164419526804 1109 20-21 0.8476825999878415 1109 22-23 0.8612975383711969 1109 24-25 0.7094506920036707 1109 26-27 0.6076843422667721 1109 28-29 0.6277250580103235 1109 30-31 0.8779040331401085 1109 32-33 0.8978774872963733 1109 34-35 1.0888973688830497 1109 36-37 0.9434078638136505 1109 38-39 1.0295776273926442 1109 40-41 1.0464384132252462 1109 42-43 1.0131359331019674 1109 44-45 1.138493222968524 1109 46-47 0.8420673495570306 1109 48-49 1.016638093380223 1109 50-51 0.9723528165030224 1109 52-53 0.8376829446440404 1109 54-55 1.052229981009944 1109 56-57 1.0176071731196856 1109 58-59 1.192569982042361 1109 60-61 0.9005986251201747 1109 62-63 1.1099408624892497 1109 64-65 0.9548444170944741 1109 66-67 0.7514500316932384 1109 68-69 0.544866630504 1109 70-71 0.6630316122990401 1109 72-73 0.6489946552511867 1109 74-75 0.7641075926353231 1109 76-77 0.7251420933190857 1110 1 0.023808972066511558 1110 2 -0.05095448634046562 1110 3 -0.26120262092224067 1110 4 -0.06186679819160901 1110 5 0.08861660214085276 1110 6 -0.0779629253293237 1110 7 -0.23541508324561988 1110 8 -0.08231901127322772 1110 9 -0.13156686261451256 1110 10-11 -0.09826041547702147 1110 12-13 0.021662414086094373 1110 14-15 0.04696084938365175 1110 16-17 0.05630750338159629 1110 18-19 0.4426858585264455 1110 20-21 0.08631136252964922 1110 22-23 0.10309426821140022 1110 24-25 0.36130873733997504 1110 26-27 0.3427921088962762 1110 28-29 0.34410372764986263 1110 30-31 0.40680592834635476 1110 32-33 0.7492059897118359 1110 34-35 0.6930222295296495 1110 36-37 0.8770756453075172 1110 38-39 1.0757314735464938 1110 40-41 1.054994711998937 1110 42-43 1.057162317345739 1110 44-45 1.1121368498730178 1110 46-47 0.8847187802519407 1110 48-49 0.8387581231089491 1110 50-51 0.7893725117835864 1110 52-53 0.7613823129086263 1110 54-55 0.9566707093637135 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-0.10114840271775805 2313 76-77 -0.010464910249083914 2314 1 -0.17500763047517154 2314 2 -0.22911676057236008 2314 3 -0.08797518252531233 2314 4 -0.1125217348016534 2314 5 -0.18942350512276107 2314 6 -0.31483504540778995 2314 7 -0.29761505181677705 2314 8 -0.10044382434762156 2314 9 -0.12917548222915798 2314 10-11 -0.11872781818700417 2314 12-13 -0.33375602045319397 2314 14-15 -0.5932447561032106 2314 16-17 -0.9659714935312778 2314 18-19 -0.6459865768543267 2314 20-21 -0.3948161219967119 2314 22-23 -0.12369654239937944 2314 24-25 0.03250407269084121 2314 26-27 0.3475032676575722 2314 28-29 0.07769546186320397 2314 30-31 -0.0917854763710153 2314 32-33 -0.08784304435804913 2314 34-35 0.1939004630257024 2314 36-37 -0.04950251367175085 2314 38-39 -0.031605007740672875 2314 40-41 -0.17454929092090055 2314 42-43 -0.15989188727632708 2314 44-45 -0.27771201285895586 2314 46-47 -0.34125589418069424 2314 48-49 -0.1717306208017142 2314 50-51 -0.27604764627309564 2314 52-53 -0.134534344886994 2314 54-55 -0.09518371931561376 2314 56-57 0.20229856602199447 2314 58-59 -0.08796517010874183 2314 60-61 0.13061853525551115 2314 62-63 0.32445642737207336 2314 64-65 0.34097055049237923 2314 66-67 0.3358017415433743 2314 68-69 0.44636392649237777 2314 70-71 0.8638165828374227 2314 72-73 0.8837129537417852 2314 74-75 0.9175259476276558 2314 76-77 1.0274774983827157 2315 1 -1.0856441983814236 2315 2 -1.2995254451891398 2315 3 -1.2788202955055397 2315 4 -0.9616935427599884 2315 5 -0.8726690290400683 2315 6 -0.9712476965562757 2315 7 -1.201481046773452 2315 8 -0.9066177693985225 2315 9 -0.9170627272476466 2315 10-11 -1.3208588675598847 2315 12-13 -1.2624979926215545 2315 14-15 -1.4670766344922086 2315 16-17 -1.375955946045515 2315 18-19 -1.3149234694448282 2315 20-21 -1.2467260454679234 2315 22-23 -1.0278855734558974 2315 24-25 -1.3916132091911209 2315 26-27 -1.2122847811054953 2315 28-29 -1.183558335733803 2315 30-31 -1.4181826899099654 2315 32-33 -1.4065291444662797 2315 34-35 -1.3074444219613568 2315 36-37 -1.5602033591693 2315 38-39 -1.541764163451763 2315 40-41 -1.3212686615498868 2315 42-43 -1.5687374727243188 2315 44-45 -1.491819570695057 2315 46-47 -1.3146147554235021 2315 48-49 -1.1178363327172391 2315 50-51 -1.1158200926122959 2315 52-53 -1.0930862861251889 2315 54-55 -1.2068349941545087 2315 56-57 -1.0644743143282938 2315 58-59 -0.8338698085335601 2315 60-61 -0.9966560399355373 2315 62-63 -1.1548060834095288 2315 64-65 -1.2186889430881003 2315 66-67 -1.006772548518871 2315 68-69 -1.158939249479893 2315 70-71 -1.1382672197589514 2315 72-73 -2.356170467584093 2315 74-75 -2.749021686462541 2315 76-77 -2.805533165104869 2316 1 -3.2152533195167905 2316 2 -3.389479751519005 2316 3 -2.6913065465504573 2316 4 -2.5764537924095876 2316 5 -3.2758958695144784 2316 6 -2.7944930156263084 2316 7 -3.0424871874496233 2316 8 -2.905045203199272 2316 9 -2.770304966551741 2316 10-11 -2.8112738817330936 2316 12-13 -3.158587946927881 2316 14-15 -3.527138355515696 2316 16-17 -4.816380702335472 2316 18-19 -4.605669715587261 2316 20-21 -3.95971574396512 2316 22-23 -3.881161753037677 2316 24-25 -3.4477837402307614 2316 26-27 -3.397568528112153 2316 28-29 -3.46150909719022 2316 30-31 -3.5238859136078204 2316 32-33 -3.6218003838520865 2316 34-35 -3.7838163319819103 2316 36-37 -3.5156988557972717 2316 38-39 -3.4702369138084492 2316 40-41 -3.42662619723432 2316 42-43 -3.6558678222463286 2316 44-45 -3.1866399072066223 2316 46-47 -2.975154154429177 2316 48-49 -2.8579763677888614 2316 50-51 -3.7280750469500497 2316 52-53 -4.740145109654602 2316 54-55 -4.135858331238033 2316 56-57 -3.801199580350932 2316 58-59 -3.6421839195182635 2316 60-61 -3.3803860764527585 2316 62-63 -3.5251931911321996 2316 64-65 -3.449558882686979 2316 66-67 -3.3826774654594693 2316 68-69 -3.5147741488846798 2316 70-71 -3.57387622776006 2316 72-73 -3.2533578242021335 2316 74-75 -3.543843280021431 2316 76-77 -3.455102312135409 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17374.0 3 2763.0 4 4794.0 5 5909.0 6 6624.0 7 6744.0 8 8475.0 9 10087.0 10 15486.0 11 18222.0 12 11346.0 13 6586.0 14 5423.0 15 5278.0 16 5203.0 17 5385.0 18 5464.0 19 5671.0 20 6010.0 21 6577.0 22 7181.0 23 7866.0 24 8824.0 25 10388.0 26 11954.0 27 13535.0 28 15533.0 29 18346.0 30 20815.0 31 25355.0 32 30716.0 33 39137.0 34 57112.0 35 73001.0 36 83722.0 37 102418.0 38 88654.0 39 24013.0 40 267.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.60867838434814 25.311244388705624 36.4596632033146 18.620414023631646 2 19.432437511745913 25.992106746852095 36.25621750297563 18.31923823842636 3 19.356378580198964 25.977397449069084 35.85110381637307 18.815120154358883 4 19.299786500410963 26.19166349257262 35.95576148186757 18.55278852514885 5 19.184942216520163 26.19663923663663 35.94591005222063 18.672508494622576 6 19.375147211378 26.126323649539195 36.1769142490591 18.321614890023703 7 19.523557145953692 26.038467754970096 35.645758861729334 18.792216237346874 8 18.941600855018915 26.412867383108363 35.522126621484986 19.12340514038774 9 18.842444616238552 26.39977045856915 35.81598094505415 18.94180398013815 10-11 19.234875177297855 26.520814642923373 35.55692827393333 18.68738190584544 12-13 19.44173843423393 26.379162043826714 34.96182593981736 19.217273582122 14-15 19.310203560045704 26.725900535209572 34.82564345420651 19.138252450538214 16-17 19.40280743646811 26.905236334434353 34.58024070363185 19.111715525465687 18-19 19.416616914363182 27.157427982366073 33.87345645175133 19.55249865151941 20-21 19.390375544352302 27.432849903174052 33.48924890503038 19.687525647443273 22-23 19.73157045007026 27.93776113240858 32.341669731288526 19.98899868623263 24-25 19.89789525649604 28.419550383284342 31.146457470348405 20.53609688987122 26-27 20.145013977628278 28.983498755107696 30.3112794635999 20.56020780366413 28-29 20.210640786235246 29.280986139152215 29.923883417785508 20.58448965682703 30-31 19.973226791750292 29.292357921587197 29.759041125752645 20.975374160909862 32-33 20.331007178921784 29.536564892190885 29.39298645651929 20.739441472368043 34-35 20.207490506253784 29.089577614429242 29.796957240242207 20.905974639074767 36-37 20.31189411085935 29.32486213526061 30.028296491097283 20.334947262782755 38-39 20.678180159772268 29.325265368308155 29.949156534725912 20.04739793719366 40-41 20.359878620523755 29.5691454574157 30.026824262865247 20.044151659195297 42-43 20.39873178634321 29.762112837436987 29.714188343886118 20.124967032333686 44-45 20.538472910971176 29.61745094255938 29.71536535921999 20.128710787249453 46-47 20.155751236580866 29.92810758488474 29.698991626648123 20.217149551886276 48-49 20.184332316922273 30.021445500673817 29.456394461885836 20.337827720518074 50-51 19.672744384340504 30.377602151460675 29.424871501980967 20.52478196221785 52-53 19.418289734723775 30.203979581989742 29.7278684785374 20.64986220474908 54-55 19.233535620350334 30.17545464456087 29.72779594030883 20.863213794779966 56-57 19.04510709638455 30.415260443847465 29.621113922686614 20.91851853708137 58-59 19.16421927309905 30.21250977286851 29.261659783894313 21.361611170138122 60-61 19.169755810901144 29.89698536694773 29.21980652570103 21.71345229645009 62-63 19.11111918636524 29.80313853105419 29.15712516219252 21.928617120388047 64-65 18.980792592959226 29.69219405013336 29.327681233604597 21.99933212330282 66-67 18.904777508848458 29.639401494071894 29.444732309508343 22.01108868757131 68-69 18.83865081573163 29.486147977150996 29.577320931305096 22.097880275812283 70-71 18.708625354326465 29.19963425173583 29.803595187615194 22.288145206322508 72-73 18.90478492085732 28.97570583961328 29.675260805049287 22.444248434480116 74-75 18.838884322882087 28.792110441778394 29.671792923546235 22.697212311793276 76-77 18.803575805945005 28.50331619074727 30.39591787859087 22.29719012471686 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 37214.0 1 21338.5 2 4965.5 3 4326.5 4 4185.0 5 4173.0 6 4073.0 7 4037.0 8 4044.5 9 4000.0 10 4051.0 11 4080.5 12 4126.0 13 4193.0 14 4324.0 15 4640.0 16 4993.0 17 5161.0 18 5536.0 19 6135.0 20 6590.0 21 7330.0 22 7839.0 23 8320.0 24 9328.0 25 10467.5 26 11080.0 27 11574.0 28 12735.5 29 13849.0 30 14295.0 31 15057.0 32 17093.5 33 18939.0 34 19871.5 35 20233.0 36 20933.0 37 22266.0 38 23075.0 39 23251.0 40 23570.0 41 24686.0 42 26490.5 43 27498.0 44 27410.0 45 27493.5 46 28042.0 47 28353.0 48 28287.0 49 28318.0 50 28897.5 51 28260.5 52 27075.0 53 26773.5 54 25074.5 55 22765.5 56 20974.0 57 20094.0 58 20549.5 59 19089.5 60 16355.0 61 15536.0 62 14215.5 63 10447.0 64 6856.0 65 5713.0 66 5132.5 67 4096.5 68 3214.5 69 2446.0 70 2104.0 71 1859.0 72 1483.5 73 1280.0 74 1207.0 75 1082.0 76 726.0 77 403.5 78 312.0 79 257.0 80 196.5 81 152.0 82 87.0 83 61.0 84 61.5 85 50.0 86 39.5 87 41.0 88 33.0 89 23.0 90 19.5 91 18.0 92 18.5 93 16.0 94 15.0 95 12.0 96 7.0 97 7.5 98 9.0 99 21.0 100 32.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011399823114832548 2 0.013529460400021046 3 0.01478218821483781 4 0.016285461592617928 5 0.013278914837057693 6 0.010773459207424167 7 0.016911825500026307 8 0.018415098877806423 9 0.01816455331484307 10-11 0.020795281725958275 12-13 0.017225007453730498 14-15 0.00826800357779064 16-17 0.007077912153714713 18-19 0.017225007453730498 20-21 0.022110645931515877 22-23 0.021484282024107494 24-25 0.01954255391114151 26-27 0.02467873795189024 28-29 0.009332822220384888 30-31 0.009019640266680697 32-33 0.01102400477038752 34-35 0.012652550929649311 36-37 0.012840460101871827 38-39 0.015283279340764516 40-41 0.012527278148167635 42-43 0.015972279638913733 44-45 0.014030551525947753 46-47 0.023864464872259347 48-49 0.023613919309295992 50-51 0.03614119745746363 52-53 0.04196638179636158 54-55 0.02718419358152377 56-57 0.021108463679662466 58-59 0.016285461592617928 60-61 0.02098319089818079 62-63 0.026244647720411195 64-65 0.025868829375966164 66-67 0.029752285601898132 68-69 0.04058838120006314 70-71 0.0553079330241601 72-73 0.08512285501679907 74-75 0.08737776508346926 76-77 0.09163703965384624 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 798258.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.13352095938037 #Duplication Level Percentage of deduplicated Percentage of total 1 80.1970067990778 45.81937368834681 2 10.384354003653891 11.8658941423477 3 3.887926477550524 6.663927866809881 4 1.8963709709816485 4.333854024693621 5 1.0202184986315925 2.9144337487357825 6 0.5972926557076297 2.0475259478253496 7 0.4065378353170327 1.6258856554406766 8 0.28378975727955047 1.2971126436470928 9 0.20254410685046972 1.041485217654626 >10 1.0287299492274373 10.450447765280646 >50 0.06261068203156626 2.467362032785616 >100 0.031085862042139336 3.2272734283210522 >500 8.7565808498775E-4 0.3510815218804476 >1k 2.189145212469375E-4 0.13845644628487905 >5k 0.0 0.0 >10k+ 4.37829042493875E-4 5.755885869945921 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29333 3.6746264992020126 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16687 2.0904269045847332 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 1107 0.1386769691002157 No Hit GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC 888 0.11124222995572859 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.2527278148167636E-4 0.0 2 0.0 0.0 0.0 0.0013780005962984398 0.0 3 0.0 0.0 0.0 0.002505455629633527 0.0 4 1.2527278148167636E-4 0.0 0.0 0.004635092914822025 0.0 5 2.505455629633527E-4 0.0 0.0 0.008769094703717345 0.0 6 2.505455629633527E-4 0.0 0.0 0.015909643248172897 0.0 7 2.505455629633527E-4 0.0 0.0 0.027309466363005443 0.0 8 2.505455629633527E-4 0.0 0.0 0.038208198351911284 0.0 9 2.505455629633527E-4 0.0 0.0 0.0487311119963721 0.0 10 2.505455629633527E-4 0.0 0.0 0.06213529961491147 0.0 11 2.505455629633527E-4 0.0 0.0 0.0779196700816027 0.0 12 3.7581834444502907E-4 0.0 0.0 0.099090770152006 0.0 13 3.7581834444502907E-4 0.0 0.0 0.12514750870019467 0.0 14 3.7581834444502907E-4 0.0 0.0 0.15646570407061375 0.0 15 3.7581834444502907E-4 0.0 0.0 0.20093754149660886 0.0 16 3.7581834444502907E-4 0.0 0.0 0.24265337773000709 0.0 17 3.7581834444502907E-4 0.0 0.0 0.2822395766782168 0.0 18 3.7581834444502907E-4 0.0 0.0 0.31430940873752594 0.0 19 5.010911259267054E-4 0.0 0.0 0.3506385153672121 0.0 20 5.010911259267054E-4 0.0 0.0 0.39260489716357366 0.0 21 5.010911259267054E-4 0.0 0.0 0.4380789168414222 0.0 22 6.263639074083817E-4 0.0 0.0 0.48843857499705606 0.0 23 7.516366888900581E-4 0.0 0.0 0.5406773248749152 0.0 24 7.516366888900581E-4 0.0 0.0 0.5917886197194391 0.0 25 7.516366888900581E-4 0.0 0.0 0.6486624625121201 0.0 26 7.516366888900581E-4 0.0 0.0 0.7116746715974034 0.0 27 7.516366888900581E-4 0.0 0.0 0.767170513793786 0.0 28 7.516366888900581E-4 0.0 0.0 0.828679449501289 0.0 29 7.516366888900581E-4 0.0 0.0 0.8801665626902581 0.0 30 7.516366888900581E-4 0.0 0.0 0.944431499590358 0.0 31 7.516366888900581E-4 0.0 0.0 1.0107008009941647 0.0 32 0.0010021822518534109 0.0 0.0 1.076469011272045 0.0 33 0.0010021822518534109 0.0 0.0 1.148375587842527 0.0 34 0.0010021822518534109 0.0 0.0 1.2330599881241404 0.0 35 0.0010021822518534109 0.0 0.0 1.3197487529094603 0.0 36 0.0010021822518534109 0.0 0.0 1.4066880632577436 0.0 37 0.0010021822518534109 0.0 0.0 1.5082842890393833 0.0 38 0.0010021822518534109 0.0 0.0 1.6058717858136091 0.0 39 0.0010021822518534109 0.0 0.0 1.696819825169306 0.0 40 0.0010021822518534109 0.0 0.0 1.7925282302213068 0.0 41 0.0010021822518534109 0.0 0.0 1.8982584577918418 0.0 42 0.0010021822518534109 0.0 0.0 2.006243595429047 0.0 43 0.0010021822518534109 0.0 0.0 2.129386739625535 0.0 44 0.0011274550333350872 0.0 0.0 2.263679161373892 0.0 45 0.0011274550333350872 0.0 0.0 2.3975957647778037 0.0 46 0.0011274550333350872 0.0 0.0 2.544415464674328 0.0 47 0.0011274550333350872 0.0 0.0 2.6987515314597537 0.0 48 0.0011274550333350872 0.0 0.0 2.850832688178509 0.0 49 0.0011274550333350872 0.0 0.0 3.0045423910565257 0.0 50 0.0011274550333350872 0.0 0.0 3.1554960927419455 0.0 51 0.0011274550333350872 0.0 0.0 3.3198539820459048 0.0 52 0.0011274550333350872 0.0 0.0 3.4790756873091158 0.0 53 0.0011274550333350872 0.0 0.0 3.6614728571464363 0.0 54 0.0011274550333350872 0.0 0.0 3.8546434861911814 0.0 55 0.0011274550333350872 0.0 0.0 4.057460119410015 0.0 56 0.0018790917222251452 0.0 0.0 4.2754347591881325 0.0 57 0.0018790917222251452 0.0 0.0 4.496791764066255 0.0 58 0.0018790917222251452 0.0 0.0 4.737691322855518 0.0 59 0.0018790917222251452 0.0 0.0 4.976335971578111 0.0 60 0.0018790917222251452 0.0 0.0 5.210220254604401 0.0 61 0.0020043645037068217 0.0 0.0 5.450117631141811 0.0 62 0.0020043645037068217 0.0 0.0 5.695025918938488 0.0 63 0.0020043645037068217 0.0 0.0 5.9539647582611135 0.0 64 0.0020043645037068217 0.0 0.0 6.220044146128195 0.0 65 0.0020043645037068217 0.0 0.0 6.5118044541990185 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTCA 55 6.0900056E-6 38.713707 2 CGGTTAC 130 1.4061516E-7 24.566776 4 GAGCGTC 75 0.001684045 23.674692 47 CGTCAAC 90 1.7563315E-4 23.656893 4 TTCGCGA 95 2.5287442E-4 22.41741 25 TCGCGAT 95 2.5287442E-4 22.41741 26 AACGATC 80 0.0024454475 22.203375 68 CGATCCG 80 0.002450798 22.195023 46 TACGTAG 80 0.0024606318 22.179728 2 GCGTCAA 100 3.582984E-4 21.291204 3 CAAACGT 110 6.789984E-4 19.365347 42 GATCCGA 95 0.00662921 18.690546 47 CGATTAA 95 0.0066508325 18.680006 17 ATCCGAA 95 0.0066532386 18.678835 33 AGTCACG 155 1.7616978E-5 18.315014 4 GACGAGT 175 2.437957E-6 18.258757 42 TAGACGA 175 2.4438032E-6 18.254179 40 GCCTAGT 175 2.4452675E-6 18.253035 17 ACTATGT 100 0.008884884 17.76493 70 CTAGCCC 100 0.008913819 17.754906 48 >>END_MODULE