FastQCFastQC Report
Thu 26 May 2016
SRR936139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936139_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798258
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172432.1600785710885453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126421.5836985034913524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97861.2259194395796849No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41660.5218864076526637No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31560.3953608983561706No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29220.36604706748945826No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23860.29890085661527976No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20120.25204883634113284No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16070.2013133598410539No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15430.1932959018262266No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10050.12589914538908473No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG9890.1238947808853779No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9500.11900914240759253No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA950.056.39280798-99
CGTATCA10300.056.059986100-101
AGTATCA26300.052.73739100-101
TCGAGTA2150.052.605797100-101
TACGCAG5850.052.404644100-101
TACCGTA800.052.08502298-99
AGGTATC15350.051.18987100-101
GTCGTAC700.051.02206498-99
ATACGCA950.050.128513100-101
AAGTATC19450.049.733864100-101
CGGTATC8000.049.110275100-101
TGAGTAC11200.047.834682100-101
GGGTATC6800.047.271927100-101
CGTACAT9150.046.516106100-101
ACGTATC2850.045.951138100-101
CGAGTAC6900.045.724106100-101
GGTATCA86900.044.9940571
CCTGTCG2400.044.63591496-97
TTTACGC2550.044.35251298-99
TGTATCA16450.044.329075100-101
Produced by FastQC (version 0.11.5)