FastQCFastQC Report
Thu 26 May 2016
SRR936138_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936138_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799350
Sequences flagged as poor quality0
Sequence length77
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294483.6839932445111656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA166922.0881966597860764No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG10310.1289797960843185No Hit
GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC8490.10621129667855132No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCGT200.00656267353.2692567
CGGTTAC1850.038.375444
GCGGTTA2350.030.210453
CGGTATA500.006729239628.39782310
CCTAGTG2650.026.79039818
GCCTAGT2950.025.26766817
CGATCCG851.1879938E-425.06003846
CTAGTGG2950.024.06595219
GCGCTCC1201.9183863E-623.663379
CACCGTC800.002456725822.185823
GGCGGTT3300.021.5135022
CGAATGT850.003488498320.88467264
GTTGTCG850.003493563320.87944633
TAGTGGC3450.020.57813320
CGATACC1054.9520994E-420.29431771
CTGCCTA4050.020.15894715
GGGCGGT3550.019.9984651
GTTACAA3550.019.9972136
GAGCGTC900.004860460319.72194537
GGTATAT900.004862219619.72070911
Produced by FastQC (version 0.11.5)