FastQCFastQC Report
Thu 26 May 2016
SRR936138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936138_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799350
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172852.1623819353224496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125601.571276662288109No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97631.221367360980797No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40550.5072871708262964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32990.4127103271408019No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29290.36642271845874774No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25260.3160067554888347No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20990.2625883530368424No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16370.204791393006818No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15280.19115531369237507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10220.1278538812785388No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG9960.12460123850628636No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9350.11697003815600177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA11500.055.90048100-101
AACGTAC650.054.94147598-99
ACCGGTA600.054.5599498-99
ATACGCA1400.053.146122100-101
AGGTATC16150.052.33659100-101
TACGCAG7000.052.295784100-101
AGTATCA25350.052.12723100-101
TAACGTA403.8380676E-852.0799498-99
ACGTATC3150.051.965096100-101
TTTACGC2400.049.59994598-99
AAGTATC19100.047.837074100-101
ACCGTAC1000.047.61594898-99
CGGTATC7950.047.544052100-101
TCGAGTA2200.047.348362100-101
CCCGTAC1650.046.89449398-99
ATATCAA16150.046.808075100-101
CGTACAT10000.046.72607100-101
TGTATCA18400.046.42198100-101
CCGGTAT2700.046.296177100-101
TGCGTAT1100.045.99267698-99
Produced by FastQC (version 0.11.5)