FastQCFastQC Report
Thu 26 May 2016
SRR936137_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936137_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3694863
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53220.1440378168283912No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG1350.002679278415.78038452
GTAACGT3301.7589628E-915.0569772
TACGTAC2402.9776365E-614.79290960
GGTAACG3402.6811904E-914.6141241
ACGCGAA1900.001700989313.08004554
TACGACC3002.0816988E-613.01687820
ATTCGCG3608.886491E-812.81878456
AACGTTC5204.7293724E-1112.2851694
CAATTCG3701.7129205E-611.5144854
AATTCGC3701.7168331E-611.51229755
GTACGAC2802.0572104E-411.41074519
CGCGGAA3752.0137886E-611.36064559
TAACGTT4104.8300535E-711.2529293
TCGCGCT3903.232144E-610.92162737
ACGCCCG3003.900692E-410.64916457
CGGTTAC3003.9075263E-410.6471464
AACGCGA2350.00832886910.57549953
TAACTCG2350.00832970510.57535654
TAAGGCG6502.1282176E-1010.37582717
GACGCAT4803.2548633E-710.35587748
Produced by FastQC (version 0.11.5)