##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936137_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3694863 Sequences flagged as poor quality 0 Sequence length 77 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.549050127163035 39.0 36.0 41.0 30.0 41.0 2 36.40335487405081 39.0 36.0 41.0 30.0 41.0 3 36.3105324879434 39.0 36.0 41.0 29.0 41.0 4 36.199610648622155 39.0 36.0 41.0 29.0 41.0 5 36.128821826411425 39.0 36.0 41.0 29.0 41.0 6 36.03841197900978 39.0 35.0 40.0 29.0 41.0 7 35.88354832100676 39.0 35.0 40.0 28.0 41.0 8 35.70396277209737 39.0 35.0 40.0 27.0 41.0 9 35.62491058531805 38.0 35.0 40.0 27.0 41.0 10-11 35.845373157272675 39.0 35.0 40.0 28.0 41.0 12-13 36.05134087515559 39.0 35.0 41.0 29.0 41.0 14-15 36.002842324600394 39.0 35.0 41.0 28.5 41.0 16-17 35.845167601613376 39.0 35.0 41.0 27.5 41.0 18-19 35.70919300661485 39.0 35.0 41.0 27.0 41.0 20-21 35.52857629633358 39.0 35.0 41.0 27.0 41.0 22-23 35.30131441950621 39.0 35.0 41.0 26.0 41.0 24-25 35.091917075139186 39.0 34.5 41.0 25.5 41.0 26-27 34.15372640338762 38.0 33.0 40.0 23.5 40.5 28-29 34.18785107864622 38.0 33.5 39.5 24.0 40.5 30-31 34.55959584969727 38.0 34.0 40.0 24.0 41.0 32-33 34.38880616141925 38.0 34.0 40.0 23.5 41.0 34-35 34.10700193755493 38.0 33.0 40.0 23.0 41.0 36-37 33.80353236371687 37.5 33.0 40.0 22.0 41.0 38-39 33.512027915514054 37.0 33.0 40.0 22.0 41.0 40-41 33.12579681574121 36.5 32.5 39.5 20.0 41.0 42-43 32.75866601819878 36.0 32.0 39.0 19.5 41.0 44-45 32.38632257813077 35.5 32.0 39.0 18.0 40.5 46-47 32.00102980814174 35.0 31.5 38.5 17.0 40.0 48-49 31.5380748893802 35.0 31.0 37.5 12.5 40.0 50-51 31.103592338876975 35.0 31.0 37.0 8.0 39.0 52-53 30.657328837361494 35.0 30.5 36.5 7.0 39.0 54-55 30.203252190947268 34.5 30.0 36.0 2.0 38.5 56-57 29.766441543299443 34.0 30.0 35.5 2.0 37.0 58-59 29.34479952301344 34.0 29.0 35.0 2.0 37.0 60-61 28.999114716837944 34.0 29.0 35.0 2.0 36.0 62-63 28.660405676746336 34.0 29.0 35.0 2.0 36.0 64-65 28.287681708361042 34.0 29.0 35.0 2.0 36.0 66-67 27.912480922838007 34.0 27.5 35.0 2.0 35.0 68-69 27.553752872569294 34.0 27.0 35.0 2.0 35.0 70-71 27.133308866932282 33.0 26.0 35.0 2.0 35.0 72-73 26.63957107475974 33.0 25.0 35.0 2.0 35.0 74-75 26.04840490702903 33.0 24.5 35.0 2.0 35.0 76-77 24.321847657139116 31.0 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.0474909264622525 1101 2 1.1542170096393818 1101 3 1.1945858036054418 1101 4 1.1535311718965602 1101 5 1.1767015113906325 1101 6 1.1425121467723116 1101 7 1.0492974451474097 1101 8 1.1781161508559919 1101 9 1.2156393584526128 1101 10-11 1.199210795385369 1101 12-13 1.2704448760954747 1101 14-15 1.2003759856982228 1101 16-17 1.1801091288224725 1101 18-19 1.1715256872307904 1101 20-21 1.1135257474292004 1101 22-23 1.2036012061251853 1101 24-25 1.2160436876081562 1101 26-27 1.0859203385502028 1101 28-29 1.2282118813407479 1101 30-31 1.2553873528152053 1101 32-33 1.321734590936508 1101 34-35 1.3231072400648998 1101 36-37 1.3238804095902594 1101 38-39 1.3812593739858414 1101 40-41 1.2918178275185994 1101 42-43 1.279054028545147 1101 44-45 1.2307212992052072 1101 46-47 1.2664006556055512 1101 48-49 1.3817089834988394 1101 50-51 1.2089443590781492 1101 52-53 1.3203094787604037 1101 54-55 1.2424977692432577 1101 56-57 1.104318201108999 1101 58-59 1.2365744709630953 1101 60-61 1.14018014689238 1101 62-63 1.0788223731153792 1101 64-65 1.020789306013075 1101 66-67 0.9739753760703742 1101 68-69 0.9613848223904746 1101 70-71 1.041625119703692 1101 72-73 1.1238362558860189 1101 74-75 1.1888248819822245 1101 76-77 1.2783323189529447 1102 1 1.0551753212697008 1102 2 1.1125031715431604 1102 3 1.0528856881069117 1102 4 1.14406153990128 1102 5 1.1711027954931055 1102 6 1.1264418933096962 1102 7 0.9946063959196749 1102 8 1.1884847689384301 1102 9 1.106883093618947 1102 10-11 1.0051053008364619 1102 12-13 1.1030165792673685 1102 14-15 1.1067562918992806 1102 16-17 1.0529645830872383 1102 18-19 1.1436494515472546 1102 20-21 1.084245024664142 1102 22-23 1.1111748325399446 1102 24-25 1.0033184732705323 1102 26-27 0.9048899066106557 1102 28-29 0.9720012363474737 1102 30-31 1.0478023948614492 1102 32-33 0.976117583440022 1102 34-35 1.064232253969429 1102 36-37 0.995846260074245 1102 38-39 1.1283064165061418 1102 40-41 1.1972654908824865 1102 42-43 1.1771903802262216 1102 44-45 1.2616224840267662 1102 46-47 1.126825463567151 1102 48-49 1.184183625281868 1102 50-51 1.3189570992625939 1102 52-53 1.2821958733245893 1102 54-55 1.2286894053199653 1102 56-57 1.2388433212596972 1102 58-59 1.2783813524299994 1102 60-61 1.2209208708841608 1102 62-63 1.2442016366069275 1102 64-65 1.1291779455734563 1102 66-67 1.1231942016031375 1102 68-69 1.2050676193469663 1102 70-71 1.279714398716326 1102 72-73 1.3708174143435556 1102 74-75 1.3837684166115096 1102 76-77 1.4866330351269994 1103 1 0.7746887039435819 1103 2 0.5912970945608507 1103 3 0.7238597725675362 1103 4 0.7419681183221911 1103 5 0.6647874793889272 1103 6 0.6858661003467788 1103 7 0.7507126824094499 1103 8 0.8517188813316565 1103 9 0.7153063736158316 1103 10-11 0.804902328366893 1103 12-13 0.7638246049713615 1103 14-15 0.8942614482213926 1103 16-17 0.8639984537629388 1103 18-19 0.86943049389793 1103 20-21 0.9648057483805914 1103 22-23 0.9076981605548724 1103 24-25 0.7851478653269197 1103 26-27 0.6803724699116955 1103 28-29 0.5413462458929672 1103 30-31 0.8848667486028461 1103 32-33 0.7484933092964852 1103 34-35 0.9112218013263558 1103 36-37 0.8370742874261907 1103 38-39 0.7710721579148725 1103 40-41 0.8374181360983357 1103 42-43 0.8145700412895778 1103 44-45 0.7580060198547116 1103 46-47 0.6282933848506147 1103 48-49 0.5957210267951467 1103 50-51 0.640472930107844 1103 52-53 0.7037344087767075 1103 54-55 0.7546305164405496 1103 56-57 0.7843756151404833 1103 58-59 0.713370236471448 1103 60-61 0.833789759399373 1103 62-63 0.7482308648491873 1103 64-65 0.638258109366916 1103 66-67 0.7942456470393715 1103 68-69 0.7409999198402453 1103 70-71 0.6146008924715289 1103 72-73 0.6821981824825087 1103 74-75 0.5605812529025123 1103 76-77 0.8027979144839144 1104 1 0.6580078893387267 1104 2 0.609671809018721 1104 3 0.5361782509484883 1104 4 0.499997551646878 1104 5 0.5979597124846947 1104 6 0.4676089579343241 1104 7 0.6693430293158755 1104 8 0.7749321906504889 1104 9 0.6690139116219882 1104 10-11 0.5895134284641372 1104 12-13 0.7553818962114534 1104 14-15 0.7700650039082646 1104 16-17 0.7407122487553579 1104 18-19 0.7576140695832763 1104 20-21 0.7209428790256354 1104 22-23 0.8647872981787117 1104 24-25 0.8105525359070427 1104 26-27 0.7325821890476902 1104 28-29 0.795372667454572 1104 30-31 0.9202466724106415 1104 32-33 0.8433029933751968 1104 34-35 0.9606425013035675 1104 36-37 0.8383177495861318 1104 38-39 0.78846468142482 1104 40-41 0.9624801688751177 1104 42-43 0.9460060455342116 1104 44-45 0.8558293806138693 1104 46-47 0.7531076692417784 1104 48-49 0.8319181120392187 1104 50-51 0.8094963957837216 1104 52-53 0.8112359033041763 1104 54-55 0.8099143822543091 1104 56-57 0.813926818046923 1104 58-59 0.8170812572432737 1104 60-61 0.6497895083340701 1104 62-63 0.7144985571074187 1104 64-65 0.7088726503856044 1104 66-67 0.8035080176826916 1104 68-69 0.6012184180972007 1104 70-71 0.7485364508456165 1104 72-73 0.6625849812442297 1104 74-75 0.5615932961275867 1104 76-77 0.6173942236708392 1105 1 0.47742445519190113 1105 2 0.5863804506435883 1105 3 0.5731324344393087 1105 4 0.6719259191241278 1105 5 0.5476831293248878 1105 6 0.3632813244744071 1105 7 0.5554842342867872 1105 8 0.6920538634610836 1105 9 0.48115363323908156 1105 10-11 0.5877629908547348 1105 12-13 0.6333289855863953 1105 14-15 0.4064664716425668 1105 16-17 0.6144286758601325 1105 18-19 0.6978568880456493 1105 20-21 0.7903187064659747 1105 22-23 0.712771441361653 1105 24-25 0.6410976346441899 1105 26-27 0.5447630871250624 1105 28-29 0.7190417729191267 1105 30-31 0.6260859200103326 1105 32-33 0.7864735958356235 1105 34-35 0.7300649620238602 1105 36-37 0.762538419368191 1105 38-39 0.7699111191103185 1105 40-41 0.6892859944556378 1105 42-43 0.5939257832263962 1105 44-45 0.5307387235652214 1105 46-47 0.47912926473073725 1105 48-49 0.481257624094404 1105 50-51 0.500952918573077 1105 52-53 0.44250408070888625 1105 54-55 0.38157206143887024 1105 56-57 0.5929129810040799 1105 58-59 0.572619866620343 1105 60-61 0.27603828774613604 1105 62-63 0.3257644867029299 1105 64-65 0.49652189002284075 1105 66-67 0.5749368608794789 1105 68-69 0.3135401421718633 1105 70-71 0.019000463245109955 1105 72-73 -0.0628764141346494 1105 74-75 -0.21390863741385502 1105 76-77 0.0019642150175229744 1106 1 0.15714385740182735 1106 2 0.3740920432936363 1106 3 -0.16605109747276003 1106 4 0.09033026956900159 1106 5 -0.018807411135981056 1106 6 0.11237648974709913 1106 7 0.2211379730514551 1106 8 0.25125177332893145 1106 9 0.12655623559737705 1106 10-11 -0.005594613722131214 1106 12-13 -0.0025895612343944663 1106 14-15 0.16634297732733216 1106 16-17 0.30875443670891656 1106 18-19 0.19371444905169 1106 20-21 0.11897803672275131 1106 22-23 0.28232223823246727 1106 24-25 0.2953358585755481 1106 26-27 0.262979628545402 1106 28-29 0.24859922090786313 1106 30-31 0.31349367598435407 1106 32-33 0.2745999410588311 1106 34-35 0.32799099902412365 1106 36-37 0.2942125339360331 1106 38-39 0.2420887072945419 1106 40-41 0.26577276656120574 1106 42-43 0.29410646247931993 1106 44-45 0.07422570222568936 1106 46-47 0.24727434582626273 1106 48-49 0.1918291741042495 1106 50-51 0.14788958927327656 1106 52-53 0.19970120988758921 1106 54-55 0.35998953399904465 1106 56-57 0.18591288785840732 1106 58-59 0.04945807392391188 1106 60-61 0.07793529064866433 1106 62-63 -0.024495132880808512 1106 64-65 0.20506758422868643 1106 66-67 0.17178300849710482 1106 68-69 0.07030706676948384 1106 70-71 -0.1429545501476035 1106 72-73 -0.14811833729172008 1106 74-75 -0.3083854493535796 1106 76-77 -0.28022472326275505 1107 1 0.4877991321944819 1107 2 0.4068828910685127 1107 3 0.45779938821478794 1107 4 0.4561047879154003 1107 5 0.34974528838317553 1107 6 0.26908164029410386 1107 7 0.16378311456241335 1107 8 0.21326043825293084 1107 9 0.2881246352488418 1107 10-11 0.39476880480821563 1107 12-13 0.3957123476306137 1107 14-15 0.30120735593771286 1107 16-17 0.39033881080127486 1107 18-19 0.4177506577843815 1107 20-21 0.24402241016968418 1107 22-23 0.21549215626260576 1107 24-25 -0.0509297124703707 1107 26-27 -0.013503493408961731 1107 28-29 0.08252332617781377 1107 30-31 0.24287999121331438 1107 32-33 0.35599843074805193 1107 34-35 0.40983132722627147 1107 36-37 0.3598001190523661 1107 38-39 0.2498641050419863 1107 40-41 0.31527041880246287 1107 42-43 0.44837381653101716 1107 44-45 0.5187081464765058 1107 46-47 0.41895807610025315 1107 48-49 0.5792281236637393 1107 50-51 0.474351210275632 1107 52-53 0.5338445199853723 1107 54-55 0.49998255526602975 1107 56-57 0.4416959985177158 1107 58-59 0.4429378396321155 1107 60-61 0.3134482073556697 1107 62-63 0.42672235348443976 1107 64-65 0.41567348347675903 1107 66-67 0.49321026024783876 1107 68-69 0.5105215518761419 1107 70-71 0.49386157435622025 1107 72-73 0.5416832327215104 1107 74-75 0.5201273947423317 1107 76-77 0.5526270774924029 1108 1 0.11355222865954318 1108 2 0.12786019872604726 1108 3 0.12408046587503208 1108 4 0.1353141722387008 1108 5 0.13061704500554328 1108 6 0.10768416267001868 1108 7 -0.004622544962046504 1108 8 0.19372897635979314 1108 9 0.12416477628491407 1108 10-11 -0.0019456649503908352 1108 12-13 0.04896081069905023 1108 14-15 0.16601365735711937 1108 16-17 0.18880504351078997 1108 18-19 0.10388843917846913 1108 20-21 0.061330971782560084 1108 22-23 0.04120558633221805 1108 24-25 0.13721500771767836 1108 26-27 -0.06834505384653511 1108 28-29 0.10306452950482026 1108 30-31 0.13363975524912064 1108 32-33 0.22396832497100405 1108 34-35 0.13722182085251688 1108 36-37 0.11820012809315017 1108 38-39 0.13706383833915936 1108 40-41 0.21324743263485146 1108 42-43 0.08818056998911317 1108 44-45 0.10547403563661817 1108 46-47 0.11943576799067301 1108 48-49 0.15111414210691976 1108 50-51 0.14684250853076364 1108 52-53 0.20065391786983255 1108 54-55 0.10670742191461713 1108 56-57 -0.006385436253101062 1108 58-59 0.019420752569462962 1108 60-61 0.07778766581331453 1108 62-63 0.046557472370619024 1108 64-65 0.11166525637251823 1108 66-67 1.1978470313067646E-4 1108 68-69 0.029854907811408538 1108 70-71 0.11438062778719527 1108 72-73 0.13602201312111362 1108 74-75 -0.05623391978619097 1108 76-77 -0.05991801336737623 1109 1 0.12221899179803586 1109 2 0.020557938228108696 1109 3 -0.118135454225758 1109 4 0.11040156869284345 1109 5 0.03652923556442289 1109 6 -0.03446994148528404 1109 7 -0.1586337763056349 1109 8 0.03922179830488659 1109 9 0.17310097813778924 1109 10-11 0.02487908869354527 1109 12-13 0.06435063349510273 1109 14-15 0.08505658437572805 1109 16-17 0.18907385115752362 1109 18-19 0.1767663364187655 1109 20-21 0.16070453900043447 1109 22-23 0.09162634465361208 1109 24-25 0.05040293279666486 1109 26-27 -0.024156692530709734 1109 28-29 0.12514884029477003 1109 30-31 0.2914286738369398 1109 32-33 0.26289934920060176 1109 34-35 0.28498057576509694 1109 36-37 0.34432287935790384 1109 38-39 0.24891322060619814 1109 40-41 0.3141271077346417 1109 42-43 0.4368882891165953 1109 44-45 0.2558651956771101 1109 46-47 0.12789362206925858 1109 48-49 0.20116603534386002 1109 50-51 0.22437815309654852 1109 52-53 0.286866252056992 1109 54-55 0.3846610582193044 1109 56-57 0.4627859651194406 1109 58-59 0.32183407304894374 1109 60-61 0.1893248734038515 1109 62-63 0.2545762437649266 1109 64-65 0.31570146999375126 1109 66-67 0.4168618435734395 1109 68-69 0.364922431673282 1109 70-71 0.35619933759956623 1109 72-73 0.18481822514505097 1109 74-75 0.08675378247155763 1109 76-77 0.31008706914283124 1110 1 -0.2189305566245423 1110 2 -0.255670888805156 1110 3 -0.3749617971256498 1110 4 -0.2694750741053795 1110 5 -0.1823627516715547 1110 6 -0.4923839522305684 1110 7 -0.48914836158232333 1110 8 -0.4446002289377091 1110 9 -0.3519906385263738 1110 10-11 -0.3195549442093366 1110 12-13 -0.2919843236935762 1110 14-15 -0.48343466950402103 1110 16-17 -0.43190509802764865 1110 18-19 -0.3160568699424573 1110 20-21 -0.5023243426612183 1110 22-23 -0.5890544262774995 1110 24-25 -0.5318660571855318 1110 26-27 -0.5470153409914147 1110 28-29 -0.4069337445292618 1110 30-31 -0.4073683495429492 1110 32-33 -0.24548047939079964 1110 34-35 -0.051459935545537405 1110 36-37 -0.21921445027199127 1110 38-39 -0.22055445374472527 1110 40-41 -0.23205852626783496 1110 42-43 -0.2287829820018601 1110 44-45 -0.18911569714705223 1110 46-47 -0.29596671212785 1110 48-49 -0.26484062579045187 1110 50-51 -0.275125072727306 1110 52-53 -0.3207060651163651 1110 54-55 -0.3408992090631422 1110 56-57 -0.4729133150793494 1110 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-0.5729610702557508 2314 22-23 -0.500091969105128 2314 24-25 -0.5275802555796716 2314 26-27 -0.4225547493492243 2314 28-29 -0.47686935316139767 2314 30-31 -0.5214204726925402 2314 32-33 -0.6175057557194705 2314 34-35 -0.7018718295965414 2314 36-37 -0.8152762556930497 2314 38-39 -0.743180984344761 2314 40-41 -0.48137446691828956 2314 42-43 -0.41612097867151476 2314 44-45 -0.548799773867465 2314 46-47 -0.44561167950056557 2314 48-49 -0.46136505352566815 2314 50-51 -0.3789133857868272 2314 52-53 -0.3211065436036762 2314 54-55 -0.46313890886612796 2314 56-57 -0.3387568754356671 2314 58-59 -0.42757075959848123 2314 60-61 -0.532650174631204 2314 62-63 -0.46772124235172186 2314 64-65 -0.3775297299199387 2314 66-67 -0.476370244391223 2314 68-69 -0.3294640217830569 2314 70-71 -0.3111647325005329 2314 72-73 -0.21928785802300155 2314 74-75 -0.17855273650907932 2314 76-77 -0.09000445679137314 2315 1 -1.3178988834027692 2315 2 -1.3174295172397237 2315 3 -1.3521083409181003 2315 4 -1.2135048947345837 2315 5 -1.2875609041888296 2315 6 -1.3008969327890583 2315 7 -1.4125341582350543 2315 8 -1.3922845003618463 2315 9 -1.3341408268776362 2315 10-11 -1.3643112855322954 2315 12-13 -1.497508735683418 2315 14-15 -1.8322844593661216 2315 16-17 -1.7154070775471197 2315 18-19 -1.5538986357064672 2315 20-21 -1.475014729819513 2315 22-23 -1.5410765449959882 2315 24-25 -1.6273132928888643 2315 26-27 -1.5748367881352365 2315 28-29 -1.4974111383422937 2315 30-31 -1.5389027861783902 2315 32-33 -1.595784057090718 2315 34-35 -1.5407416980227495 2315 36-37 -1.8528253538840822 2315 38-39 -1.6586156455564236 2315 40-41 -1.6498549576163484 2315 42-43 -1.784416894610981 2315 44-45 -1.7373884869880918 2315 46-47 -1.6829243074994729 2315 48-49 -1.633308070452543 2315 50-51 -1.5441192746290078 2315 52-53 -1.508687620389889 2315 54-55 -1.5757628504941437 2315 56-57 -1.7246090165390378 2315 58-59 -1.5063747617114096 2315 60-61 -1.6946865891851388 2315 62-63 -1.895209640184003 2315 64-65 -1.8201218219050652 2315 66-67 -1.6871782232669474 2315 68-69 -1.8031987206896822 2315 70-71 -1.8497355416965924 2315 72-73 -3.132218879084526 2315 74-75 -3.740822145833686 2315 76-77 -3.311118868766574 2316 1 -3.4632111278031843 2316 2 -3.378985128052392 2316 3 -2.868434143323043 2316 4 -2.9032988497201373 2316 5 -3.383327755804771 2316 6 -3.0325561701291193 2316 7 -3.239113585915611 2316 8 -3.0981989881199254 2316 9 -2.7854640321173676 2316 10-11 -2.7483725907846477 2316 12-13 -2.798410743193685 2316 14-15 -2.975231667030691 2316 16-17 -4.309472311968925 2316 18-19 -3.9435102726108617 2316 20-21 -3.2879234916607913 2316 22-23 -3.4572866416853927 2316 24-25 -3.5363216035744856 2316 26-27 -3.224427184676305 2316 28-29 -3.409008532280854 2316 30-31 -3.535125867478534 2316 32-33 -3.718088662641243 2316 34-35 -3.6756762803288225 2316 36-37 -3.6124206457838177 2316 38-39 -3.677759044077753 2316 40-41 -3.6901681086546887 2316 42-43 -3.7532185234479982 2316 44-45 -3.5972534368276783 2316 46-47 -3.318470657722763 2316 48-49 -3.4390059616807847 2316 50-51 -3.9023922606349437 2316 52-53 -4.852611845876819 2316 54-55 -4.0707066425887 2316 56-57 -3.971287999383648 2316 58-59 -4.000767127139486 2316 60-61 -3.756847496270307 2316 62-63 -3.718254182600436 2316 64-65 -3.716417574002545 2316 66-67 -3.835177142107298 2316 68-69 -3.870018118171661 2316 70-71 -3.7630328817473107 2316 72-73 -3.71724398542003 2316 74-75 -3.7331286971312814 2316 76-77 -3.8344361439136847 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93225.0 3 7781.0 4 9503.0 5 10914.0 6 12671.0 7 13797.0 8 15444.0 9 16198.0 10 18420.0 11 19861.0 12 17525.0 13 15904.0 14 16347.0 15 16849.0 16 17768.0 17 18677.0 18 19822.0 19 21176.0 20 22781.0 21 24529.0 22 27371.0 23 31336.0 24 36826.0 25 43051.0 26 50879.0 27 59983.0 28 70465.0 29 83679.0 30 99927.0 31 119645.0 32 147607.0 33 185067.0 34 244660.0 35 328507.0 36 467123.0 37 616732.0 38 533599.0 39 138239.0 40 975.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.29393078822774 26.174578754023532 27.570106612070433 22.961383845678295 2 23.024102607966928 26.42394972677359 27.717484866169407 22.834462799090076 3 23.157449674312243 26.599637932229232 27.199962320698496 23.04295007276003 4 23.057991594605085 26.46825076726433 27.44026328169875 23.033494356431838 5 23.024967017594765 26.67409052743156 27.462744904847725 22.838197550125948 6 23.254047651528438 26.570204044511712 27.259771854719787 22.91597644924006 7 23.338108316320426 26.274100264897864 27.272732194375372 23.115059224406334 8 23.102382952298996 26.56478440928602 27.282639619576532 23.050193018838456 9 23.006788700315383 26.44417898521125 27.30752603995369 23.241506274519676 10-11 23.351920948023842 26.458568757729328 27.24079697866329 22.948713315583536 12-13 23.381135812013458 26.35561151617595 27.20192341398403 23.061329257826557 14-15 23.191896630777883 26.489310505548424 27.313129404784654 23.005663458889035 16-17 23.435294722660736 26.32536385701632 27.274108889485856 22.965232530837092 18-19 23.285614148494005 26.3297508416196 27.10766951530859 23.27696549457781 20-21 23.226752964339408 26.421375755593036 27.166279262882014 23.18559201718554 22-23 23.36323784031367 26.418765423275868 26.96475886361249 23.25323787279797 24-25 23.352972871937045 26.504674197555143 26.909542579801897 23.23281035070592 26-27 23.35154319065937 26.595383828238994 26.89918976060986 23.153883220491775 28-29 23.361993166408787 26.553212866488106 26.84653733269076 23.238256634412345 30-31 23.275247809358905 26.490344424659906 26.8782699582233 23.356137807757886 32-33 23.250642481648555 26.65793017037521 26.83806457960676 23.25336276836948 34-35 23.23416371378279 26.497106033952367 26.971971986874227 23.29675826539061 36-37 23.286263336641923 26.503013863854935 27.02110503198466 23.18961776751848 38-39 23.202410613346753 26.550068151744448 27.037937466054395 23.2095837688544 40-41 23.088124953308164 26.442967207985195 27.03672495612321 23.43218288258343 42-43 23.09913917537703 26.464479869225794 26.96830920769568 23.468071747701497 44-45 23.081173852439747 26.445244675752704 27.078820528166197 23.394760943641348 46-47 23.08603774810772 26.244355650846245 27.083983042588443 23.58562355845759 48-49 23.070011683773632 26.430612569748014 26.902037676109337 23.59733807036902 50-51 22.995898912490954 26.514874310703913 26.889258453797275 23.599968323007854 52-53 23.134483030773893 26.356237798357697 26.960422580076145 23.548856590792266 54-55 22.968300811870215 26.3774437120193 27.007049889044314 23.64720558706617 56-57 22.994451406016477 26.499970628753122 26.910180020026587 23.59539794520381 58-59 23.079088608828467 26.433213304215954 26.949408437589035 23.538289649366547 60-61 23.082689049105916 26.328281701037593 26.894727559844632 23.69430169001186 62-63 22.954244466270264 26.262584287791846 27.118375415415503 23.664795830522387 64-65 22.98394088497918 26.234490069868205 27.006847942010083 23.774721103142532 66-67 22.924262687407616 26.28486228348972 27.063067501935667 23.727807527167 68-69 22.877453320340475 26.154079632069976 27.19169872236592 23.776768325223628 70-71 22.87640052680158 26.071424751306683 27.22677854768869 23.82539617420305 72-73 22.930086497298248 25.85203847791626 27.221308153803314 23.99656687098218 74-75 22.795800168458086 25.862419937376064 27.20474835642307 24.13703153774278 76-77 22.806350766350132 25.797729725234365 27.340195690549468 24.05572381786603 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5091.0 1 3143.5 2 1134.0 3 1116.0 4 1160.0 5 1235.5 6 1344.0 7 1470.5 8 1588.0 9 1612.0 10 1720.0 11 1881.5 12 2071.5 13 2208.0 14 2296.5 15 2573.5 16 3017.0 17 3272.0 18 3566.5 19 4271.0 20 5233.0 21 6676.0 22 7567.0 23 8900.0 24 12086.0 25 16717.0 26 19495.0 27 23145.0 28 32338.5 29 42867.0 30 47852.0 31 54435.0 32 67518.0 33 80488.0 34 93568.0 35 100178.0 36 106983.5 37 119232.5 38 129066.0 39 133456.0 40 138438.0 41 148273.5 42 158520.0 43 163913.0 44 167139.0 45 174336.5 46 181056.0 47 184796.0 48 185788.0 49 186050.0 50 184352.5 51 179606.0 52 176819.0 53 174315.5 54 164968.0 55 149981.5 56 134445.0 57 127051.0 58 119115.5 59 100885.0 60 81978.5 61 73367.0 62 64893.0 63 48599.5 64 35194.5 65 29609.0 66 24899.5 67 17482.0 68 12588.5 69 8817.5 70 7232.0 71 6175.5 72 4244.5 73 2830.5 74 2291.0 75 1957.5 76 1350.5 77 880.5 78 684.0 79 541.0 80 337.5 81 245.0 82 187.5 83 162.0 84 139.0 85 108.0 86 98.0 87 96.0 88 91.5 89 85.0 90 72.5 91 62.0 92 61.0 93 52.0 94 51.0 95 58.0 96 58.0 97 58.0 98 59.0 99 125.5 100 191.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009391417218987551 2 0.0132887200418527 3 0.014263045747568991 4 0.015399759070904658 5 0.012693298777248304 6 0.01014922610121133 7 0.0164823431883672 8 0.017970896349878196 9 0.017213087467654417 10-11 0.01993308006277905 12-13 0.01619816485753328 14-15 0.008146445483905628 16-17 0.00664436002092635 18-19 0.017497265798488332 20-21 0.022395958930006336 22-23 0.020866808864090498 24-25 0.0189316897541262 26-27 0.024168690422351247 28-29 0.008647140638232053 30-31 0.009188432696963324 32-33 0.010812308873157138 34-35 0.012233200527326724 36-37 0.011800166880341707 38-39 0.014438965666656653 40-41 0.011556585453912634 42-43 0.015061451534197615 44-45 0.013924738210861947 46-47 0.02443933645171688 48-49 0.02303197709901558 50-51 0.0360094542070978 52-53 0.03941959417710481 54-55 0.02830957467164547 56-57 0.020948002672900187 58-59 0.01679358612213768 60-61 0.021015664180241596 62-63 0.0262797294514032 64-65 0.026509778576363996 66-67 0.028228380862835783 68-69 0.04200426375754662 70-71 0.05444044880689757 72-73 0.08625488955882803 74-75 0.0850775793310875 76-77 0.09124830880062401 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3694863.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.03830938010279 #Duplication Level Percentage of deduplicated Percentage of total 1 72.9962042490489 40.17587673032327 2 13.703189762080948 15.084007952393366 3 5.418475999857115 8.94671275346393 4 2.683631330007011 5.908101256122505 5 1.4966564139497878 4.118671937334181 6 0.9068755766266461 2.9947739133381877 7 0.6346811874845081 2.4452245688152368 8 0.4360043706864259 1.9197554755933222 9 0.3035958076895259 1.5038459991106483 >10 1.3421598670781938 12.565498206213075 >50 0.06065923088994644 2.250318496506523 >100 0.01707649628983435 1.634695953921655 >500 6.909947737475674E-4 0.2696954247976621 >1k 4.935676871405738E-5 0.03824850453932947 >5k 4.935676871405738E-5 0.14457282752720985 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5322 0.1440378168283912 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.4129205873127095E-5 0.0 2 0.0 0.0 0.0 3.789044411118897E-4 8.119380880969064E-5 3 0.0 0.0 0.0 7.307442792872158E-4 1.0825841174625419E-4 4 2.7064602936563548E-5 0.0 0.0 0.0016509407791303764 1.3532301468281775E-4 5 1.0825841174625419E-4 0.0 0.0 0.003112429337704808 1.3532301468281775E-4 6 1.0825841174625419E-4 0.0 0.0 0.005954212646043981 1.3532301468281775E-4 7 1.0825841174625419E-4 0.0 0.0 0.012070812909707343 1.3532301468281775E-4 8 1.0825841174625419E-4 0.0 0.0 0.019486514114325754 1.3532301468281775E-4 9 1.0825841174625419E-4 0.0 0.0 0.026496246274895715 1.3532301468281775E-4 10 1.0825841174625419E-4 0.0 0.0 0.035265177626342306 1.3532301468281775E-4 11 1.8945222055594485E-4 0.0 0.0 0.04533320991874394 1.3532301468281775E-4 12 2.4358142642907194E-4 0.0 0.0 0.05848660694591383 1.3532301468281775E-4 13 2.4358142642907194E-4 0.0 0.0 0.07651163250166515 1.3532301468281775E-4 14 2.97710632302199E-4 0.0 0.0 0.09892112373313977 1.6238761761938129E-4 15 2.97710632302199E-4 0.0 0.0 0.12601279127263987 1.6238761761938129E-4 16 2.97710632302199E-4 0.0 0.0 0.15245490834166248 1.6238761761938129E-4 17 3.2477523523876257E-4 0.0 0.0 0.17830160414608065 1.6238761761938129E-4 18 3.789044411118897E-4 0.0 0.0 0.2066923726265358 1.6238761761938129E-4 19 4.3303364698501676E-4 0.0 0.0 0.2352184641216738 2.4358142642907194E-4 20 4.600982499215803E-4 0.0 0.0 0.26756066463086725 2.4358142642907194E-4 21 4.600982499215803E-4 0.0 0.0 0.3038272325658624 2.97710632302199E-4 22 6.766150734140887E-4 0.0 0.0 0.3434498112649914 3.2477523523876257E-4 23 8.119380880969064E-4 0.0 0.0 0.3851022351843627 3.789044411118897E-4 24 8.119380880969064E-4 0.0 0.0 0.42702530513309966 4.059690440484532E-4 25 8.119380880969064E-4 0.0 0.0 0.47046399284628415 4.059690440484532E-4 26 8.119380880969064E-4 0.0 0.0 0.5175293373529681 4.600982499215803E-4 27 8.119380880969064E-4 0.0 0.0 0.5629978702863949 4.600982499215803E-4 28 8.119380880969064E-4 0.0 0.0 0.610117343998952 5.41292058731271E-4 29 8.119380880969064E-4 0.0 0.0 0.6592125337258783 5.41292058731271E-4 30 8.119380880969064E-4 0.0 0.0 0.7146408405399605 5.95421264604398E-4 31 8.119380880969064E-4 0.0 0.0 0.7738581917651616 5.95421264604398E-4 32 8.660672939700335E-4 0.0 0.0 0.836025584710448 5.95421264604398E-4 33 8.660672939700335E-4 0.0 0.0 0.9034435106254278 6.224858675409616E-4 34 8.660672939700335E-4 0.0 0.0 0.975056449995575 6.224858675409616E-4 35 8.660672939700335E-4 0.0 0.0 1.049781818703427 6.224858675409616E-4 36 8.660672939700335E-4 0.0 0.0 1.1278631981754128 6.495504704775251E-4 37 8.931318969065971E-4 0.0 0.0 1.2101125264996293 6.495504704775251E-4 38 8.931318969065971E-4 0.0 0.0 1.2890329086626486 7.036796763506523E-4 39 8.931318969065971E-4 0.0 0.0 1.3730143715748053 7.036796763506523E-4 40 9.472611027797242E-4 0.0 0.0 1.4652234737796774 7.307442792872158E-4 41 9.472611027797242E-4 0.0 0.0 1.5599495840576498 7.848734851603428E-4 42 0.0010013903086528513 0.0 0.0 1.6644460159957217 7.848734851603428E-4 43 0.0010013903086528513 0.0 0.0 1.7729209445654683 8.3900269103347E-4 44 0.0010555195145259785 0.0 0.0 1.8901377398837251 8.3900269103347E-4 45 0.0010555195145259785 0.0 0.0 2.0105752229514327 8.3900269103347E-4 46 0.0010555195145259785 0.0 0.0 2.141919741002576 8.3900269103347E-4 47 0.001082584117462542 0.0 0.0 2.278893696464524 8.3900269103347E-4 48 0.001082584117462542 0.0 0.0 2.413079997824006 9.201964998431606E-4 49 0.001190842529208796 0.0 0.0 2.553951256108819 9.472611027797242E-4 50 0.001190842529208796 0.0 0.0 2.6964193259668896 9.472611027797242E-4 51 0.0012449717350819232 0.0 0.0 2.8514995007933988 9.472611027797242E-4 52 0.0012449717350819232 0.0 0.0 3.0164041264858805 9.472611027797242E-4 53 0.0012449717350819232 0.0 0.0 3.1881831613242495 9.472611027797242E-4 54 0.0012449717350819232 0.0 0.0 3.37254723652812 9.743257057162878E-4 55 0.0012449717350819232 0.0 0.0 3.560889808363666 9.743257057162878E-4 56 0.0015156177644475588 0.0 0.0 3.762115131197016 9.743257057162878E-4 57 0.0015156177644475588 0.0 0.0 3.9625014513393326 0.0010013903086528513 58 0.0015156177644475588 0.0 0.0 4.175256295023659 0.001082584117462542 59 0.0015156177644475588 0.0 0.0 4.387009748399332 0.0011096487203991054 60 0.0015156177644475588 0.0 0.0 4.613269828949003 0.0011096487203991054 61 0.00167800538206694 0.0 0.0 4.845565315953528 0.0011096487203991054 62 0.00167800538206694 0.0 0.0 5.089471517617839 0.0011096487203991054 63 0.00167800538206694 0.0 0.0 5.337491538928507 0.0012449717350819232 64 0.00167800538206694 0.0 0.0 5.597636502354756 0.0012449717350819232 65 0.0017050699850035035 0.0 0.0 5.870204118528887 0.0012449717350819232 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCG 135 0.0026792784 15.780384 52 GTAACGT 330 1.7589628E-9 15.056977 2 TACGTAC 240 2.9776365E-6 14.792909 60 GGTAACG 340 2.6811904E-9 14.614124 1 ACGCGAA 190 0.0017009893 13.080045 54 TACGACC 300 2.0816988E-6 13.016878 20 ATTCGCG 360 8.886491E-8 12.818784 56 AACGTTC 520 4.7293724E-11 12.285169 4 CAATTCG 370 1.7129205E-6 11.51448 54 AATTCGC 370 1.7168331E-6 11.512297 55 GTACGAC 280 2.0572104E-4 11.410745 19 CGCGGAA 375 2.0137886E-6 11.360645 59 TAACGTT 410 4.8300535E-7 11.252929 3 TCGCGCT 390 3.232144E-6 10.921627 37 ACGCCCG 300 3.900692E-4 10.649164 57 CGGTTAC 300 3.9075263E-4 10.647146 4 AACGCGA 235 0.008328869 10.575499 53 TAACTCG 235 0.008329705 10.575356 54 TAAGGCG 650 2.1282176E-10 10.375827 17 GACGCAT 480 3.2548633E-7 10.355877 48 >>END_MODULE