FastQCFastQC Report
Thu 26 May 2016
SRR936137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936137_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3694863
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65140.17629882352877493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45530.12322513717017383No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14250.040.728058100-101
GGTATCA80250.036.479641
CGTACAT18800.035.145447100-101
CGGTATC12050.031.121277100-101
GTATCAA153150.030.7707921
TTATACG9100.028.12311496-97
CCTGTCG12800.027.8982996-97
ACGTATC5300.026.393436100-101
TACGCAG11350.025.960512100-101
CCGTACA9600.025.112371100-101
TCGTACA9800.024.903576100-101
AGTATCA36250.024.631294100-101
CGAGTAC19150.024.400843100-101
TATCAAC194550.024.1586932
TCAACGC195750.023.918774
ATCAACG196150.023.8699913
TCGAGTA8400.023.739386100-101
GCGTACA8250.023.449488100-101
CAACGCA202550.023.1448275
TCGTATC7350.022.67643100-101
Produced by FastQC (version 0.11.5)