FastQCFastQC Report
Thu 26 May 2016
SRR936136_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936136_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3705428
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53950.14559721576023066No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCT3550.017.99742337
ATTCGCG3750.017.03940256
CGCGGAA3850.016.5990659
CGGTTAC3258.0035534E-1116.3818284
CGATCGT4450.015.95255851
CAATTCG4900.015.9392954
CTCGCGC4251.8189894E-1215.03273736
AATTCGC4550.014.82425555
TAACGCG1707.341509E-414.61930452
CGCGCTT4800.014.05064238
GCGCGAG4303.8198777E-1013.20848224
ACGATCG3506.1369064E-813.18703159
AGCGCGA4102.6502676E-912.98734923
TAAGGCG7700.012.90759217
TCGCGGA5052.910383E-1112.65421658
CCTCGCG5407.2759576E-1212.48844735
CGCTCCG6000.012.42305425
ATCGTTC6000.012.4228873
GACGCAT3153.5928006E-612.39806448
AACGCGA2601.0309888E-412.28935653
Produced by FastQC (version 0.11.5)