##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936136_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3705428 Sequences flagged as poor quality 0 Sequence length 77 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.53059970400181 39.0 36.0 41.0 30.0 41.0 2 36.3949800131051 39.0 36.0 41.0 30.0 41.0 3 36.25335076002016 39.0 36.0 41.0 29.0 41.0 4 36.15572020290234 39.0 36.0 41.0 29.0 41.0 5 36.082731603474684 39.0 35.0 41.0 29.0 41.0 6 36.0006325854935 39.0 35.0 40.0 28.0 41.0 7 35.86575369970756 39.0 35.0 40.0 28.0 41.0 8 35.73740388424765 39.0 35.0 40.0 27.0 41.0 9 35.91768535240733 39.0 35.0 40.0 28.0 41.0 10-11 36.15905733426746 39.0 35.0 41.0 29.5 41.0 12-13 36.089346763720684 39.0 35.0 41.0 29.0 41.0 14-15 35.94618705315553 39.0 35.0 41.0 28.0 41.0 16-17 35.807414420142564 39.0 35.0 41.0 27.0 41.0 18-19 35.666974908161755 39.0 35.0 41.0 27.0 41.0 20-21 35.4827696017842 39.0 35.0 41.0 26.5 41.0 22-23 35.2486097422484 39.0 35.0 41.0 26.0 41.0 24-25 35.039479784791396 39.0 34.0 41.0 25.0 41.0 26-27 34.077739197739106 38.0 33.0 40.0 23.5 40.5 28-29 34.08979043716407 38.0 33.0 39.5 23.5 40.5 30-31 34.49699171053924 38.0 34.0 40.0 24.0 41.0 32-33 34.364594049594274 38.0 34.0 40.0 23.0 41.0 34-35 34.09876133067489 38.0 33.0 40.0 23.0 41.0 36-37 33.77969845858562 37.5 33.0 40.0 22.0 41.0 38-39 33.458924718008284 37.0 33.0 40.0 21.5 41.0 40-41 33.093218920999135 36.5 32.0 39.5 20.0 41.0 42-43 32.71798116169036 36.0 32.0 39.0 18.5 41.0 44-45 32.35327052637374 35.5 32.0 39.0 18.0 40.5 46-47 31.943008068163785 35.0 31.0 38.5 15.5 40.0 48-49 31.51797025876633 35.0 31.0 37.5 12.0 40.0 50-51 31.073608635763534 35.0 31.0 37.0 7.5 39.0 52-53 30.596964777078384 35.0 30.0 36.5 6.5 39.0 54-55 30.14096306823395 34.0 30.0 36.0 2.0 38.5 56-57 29.711780528457172 34.0 29.5 35.5 2.0 37.0 58-59 29.292705458046953 34.0 29.0 35.0 2.0 37.0 60-61 28.940612393494085 34.0 29.0 35.0 2.0 36.0 62-63 28.57737945522083 34.0 29.0 35.0 2.0 36.0 64-65 28.20380074852352 34.0 28.5 35.0 2.0 35.5 66-67 27.853672774103288 34.0 27.5 35.0 2.0 35.0 68-69 27.493320744594147 34.0 27.0 35.0 2.0 35.0 70-71 27.047654548948195 33.0 26.0 35.0 2.0 35.0 72-73 26.56527086749493 33.0 25.0 35.0 2.0 35.0 74-75 25.992482379903215 33.0 24.5 35.0 2.0 35.0 76-77 24.20278521131702 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.2635711453594993 1101 2 1.1516088858043219 1101 3 1.3314935013674116 1101 4 1.1766008207105259 1101 5 1.273080492209047 1101 6 1.2308716336350614 1101 7 1.2388384319828987 1101 8 1.3037050989226628 1101 9 1.2143825932276044 1101 10-11 1.2846565691803775 1101 12-13 1.4462278895057494 1101 14-15 1.499223485204844 1101 16-17 1.4649520417507844 1101 18-19 1.5419367754143636 1101 20-21 1.5051758007219505 1101 22-23 1.428850313549269 1101 24-25 1.4576382177386122 1101 26-27 1.588851496791868 1101 28-29 1.5950483553784593 1101 30-31 1.5235315096020372 1101 32-33 1.530246558219119 1101 34-35 1.5582689859032115 1101 36-37 1.6205946154576125 1101 38-39 1.5436400832662613 1101 40-41 1.483786817671195 1101 42-43 1.5907150924417195 1101 44-45 1.5443227363263219 1101 46-47 1.5754056156117144 1101 48-49 1.4633092112381227 1101 50-51 1.433841128353901 1101 52-53 1.56999683117904 1101 54-55 1.7125180332138719 1101 56-57 1.677139787241174 1101 58-59 1.7753648963955904 1101 60-61 1.6233317021944877 1101 62-63 1.6552016858721252 1101 64-65 1.7412735519500337 1101 66-67 1.7484077002945178 1101 68-69 1.7354351294608037 1101 70-71 1.7806476136432146 1101 72-73 1.6836308098443453 1101 74-75 1.618358888774555 1101 76-77 1.8030808551774165 1102 1 0.8136935146755633 1102 2 0.791339092225634 1102 3 0.7836248808934556 1102 4 0.5931157220801779 1102 5 0.6947126869608411 1102 6 0.688611834689894 1102 7 0.6414934701464006 1102 8 0.6028409938081083 1102 9 0.642305869717319 1102 10-11 0.7546748049429794 1102 12-13 0.8933187982859181 1102 14-15 0.77336467820313 1102 16-17 0.7289968261859556 1102 18-19 0.7412212738198036 1102 20-21 0.8460617648246611 1102 22-23 0.7343509510318782 1102 24-25 0.8031866065995885 1102 26-27 0.6215940050893991 1102 28-29 0.7366267486488383 1102 30-31 0.8967512582322215 1102 32-33 0.8663832768756592 1102 34-35 0.7516933442333809 1102 36-37 0.7447307667033485 1102 38-39 0.7842970321685243 1102 40-41 0.7151475439570092 1102 42-43 0.7869361707273157 1102 44-45 0.6923262023175596 1102 46-47 0.8030416831130864 1102 48-49 0.7023948424142752 1102 50-51 0.7030437110984629 1102 52-53 0.7146177084781655 1102 54-55 0.7811073166697433 1102 56-57 0.850112831810268 1102 58-59 0.929312845000478 1102 60-61 0.7567171621219551 1102 62-63 0.6480656405886371 1102 64-65 0.5822300151349857 1102 66-67 0.5147164025303219 1102 68-69 0.5167144186249679 1102 70-71 0.5689689493145558 1102 72-73 0.5706512879050294 1102 74-75 0.6141961102390887 1102 76-77 0.5431495426468693 1103 1 0.6541043413024425 1103 2 0.6651958862488243 1103 3 0.6167912142215002 1103 4 0.6935412579352445 1103 5 0.6429162298742241 1103 6 0.6495249047386267 1103 7 0.601962750082059 1103 8 0.5563488537212251 1103 9 0.6544869493111705 1103 10-11 0.769937613347345 1103 12-13 0.6921256245847474 1103 14-15 0.6786462435189762 1103 16-17 0.6861831505720559 1103 18-19 0.629417581168596 1103 20-21 0.6588350826551093 1103 22-23 0.5325891505800158 1103 24-25 0.7132767799885826 1103 26-27 0.6614279707078978 1103 28-29 0.6662299632501245 1103 30-31 0.6994614202982845 1103 32-33 0.6761453035215865 1103 34-35 0.8297094635368651 1103 36-37 0.9117735967482687 1103 38-39 0.7905525878122646 1103 40-41 0.707885193231867 1103 42-43 0.7819089745284344 1103 44-45 0.8119731080885515 1103 46-47 0.8407759130215027 1103 48-49 0.958314413888953 1103 50-51 0.9084910859245205 1103 52-53 0.9457395979790562 1103 54-55 1.0035025464882033 1103 56-57 1.0994071327780084 1103 58-59 0.9242170299875418 1103 60-61 0.8658007388453974 1103 62-63 0.8150639924143306 1103 64-65 0.6907502561970098 1103 66-67 0.8620378877919883 1103 68-69 0.8347100415713662 1103 70-71 0.6896019818494601 1103 72-73 0.717487944042432 1103 74-75 0.7449093292319837 1103 76-77 0.9125168474188996 1104 1 0.5334002348249598 1104 2 0.5002653712083287 1104 3 0.6383022835858867 1104 4 0.6651376034114307 1104 5 0.523161148312532 1104 6 0.6738188729851871 1104 7 0.5652906187432336 1104 8 0.4662294088230112 1104 9 0.48468004087651906 1104 10-11 0.5448981354666245 1104 12-13 0.6475304478871919 1104 14-15 0.7238542511111845 1104 16-17 0.6491889244552027 1104 18-19 0.5048050672638311 1104 20-21 0.41917930838301487 1104 22-23 0.541376382305458 1104 24-25 0.5929854410077766 1104 26-27 0.4669750991296411 1104 28-29 0.4573387374522042 1104 30-31 0.6237898101452757 1104 32-33 0.6509322104782456 1104 34-35 0.7103477022399645 1104 36-37 0.704277709837811 1104 38-39 0.684312116955887 1104 40-41 0.7745799611486035 1104 42-43 0.6949808203819003 1104 44-45 0.634098758598455 1104 46-47 0.6475217201983057 1104 48-49 0.6495290782686247 1104 50-51 0.5644758742997418 1104 52-53 0.4652979221721907 1104 54-55 0.46103732786242446 1104 56-57 0.4366999225223154 1104 58-59 0.5061654773205468 1104 60-61 0.48432505371942014 1104 62-63 0.4863751442349802 1104 64-65 0.49381786255392157 1104 66-67 0.6110832717970318 1104 68-69 0.46062722164495185 1104 70-71 0.4406266078791923 1104 72-73 0.4777740711650935 1104 74-75 0.35016528397438407 1104 76-77 0.41807535247129124 1105 1 0.21979345179245513 1105 2 0.5084013484481957 1105 3 0.3984184968472775 1105 4 0.505890057340217 1105 5 0.36952878062586336 1105 6 0.3957678934400377 1105 7 0.44688006180736295 1105 8 0.3577661616126022 1105 9 0.3523888793112846 1105 10-11 0.5043555653388836 1105 12-13 0.5469405847693878 1105 14-15 0.4825747752389944 1105 16-17 0.4212678468673303 1105 18-19 0.4077615824107994 1105 20-21 0.38030837070973433 1105 22-23 0.29407895807842266 1105 24-25 0.35133442503572354 1105 26-27 0.2642664204201637 1105 28-29 0.3802218171083993 1105 30-31 0.5858848528920504 1105 32-33 0.5346949902895446 1105 34-35 0.4679444278238094 1105 36-37 0.46063954436146304 1105 38-39 0.43419399175891016 1105 40-41 0.4137894926004222 1105 42-43 0.4694403648262977 1105 44-45 0.3443740058031466 1105 46-47 0.29808307115958854 1105 48-49 0.22445561655429813 1105 50-51 0.2873372360842019 1105 52-53 0.23227946221961204 1105 54-55 0.01815235057440745 1105 56-57 0.17899663394543808 1105 58-59 0.23860344311644965 1105 60-61 0.3583457827247223 1105 62-63 0.18752089904664615 1105 64-65 0.2677068062738819 1105 66-67 0.23675429681859228 1105 68-69 0.06521415058531588 1105 70-71 0.22216102915631808 1105 72-73 -0.13600874344247416 1105 74-75 -0.43680706829283267 1105 76-77 -0.5301656667953942 1106 1 0.09756832113954772 1106 2 0.30265037972621656 1106 3 0.29219211890672625 1106 4 0.40165094810194546 1106 5 0.2838085077673824 1106 6 0.21385294453543935 1106 7 -0.07355094854807476 1106 8 0.1084781311398757 1106 9 0.24646311867322623 1106 10-11 0.16556820928832394 1106 12-13 0.3980983070581203 1106 14-15 0.4592318774598496 1106 16-17 0.37562276886179546 1106 18-19 0.3536125630048872 1106 20-21 0.2336313242830741 1106 22-23 0.3294229467574681 1106 24-25 0.10192922294985607 1106 26-27 0.07671388391101175 1106 28-29 0.3378043819723686 1106 30-31 0.5217171241713956 1106 32-33 0.3811991042885836 1106 34-35 0.4268440111553602 1106 36-37 0.4845162106895984 1106 38-39 0.6410128551728107 1106 40-41 0.5826435613757468 1106 42-43 0.5619598095925724 1106 44-45 0.5076081282747822 1106 46-47 0.5233025265583322 1106 48-49 0.4219595155201823 1106 50-51 0.4067529895751498 1106 52-53 0.44769428787349597 1106 54-55 0.40027639657338554 1106 56-57 0.48794921269835 1106 58-59 0.37158285381118006 1106 60-61 0.14304169199312966 1106 62-63 0.16695436058932245 1106 64-65 0.30968952638129466 1106 66-67 0.09972608837852093 1106 68-69 0.23135408138373847 1106 70-71 0.18579980492632586 1106 72-73 0.04889843232352575 1106 74-75 0.302323148823902 1106 76-77 0.24573009409536795 1107 1 -0.1270838041891622 1107 2 -0.037181041652097235 1107 3 0.01779093005821153 1107 4 -0.0499082883833708 1107 5 -0.04577799083246248 1107 6 -0.2361422420660091 1107 7 -0.16868685698870678 1107 8 -0.1696846677478021 1107 9 -0.31966399038741855 1107 10-11 -0.12716159393507098 1107 12-13 -0.04794029639825936 1107 14-15 -0.28414490136510295 1107 16-17 -0.21775373578222457 1107 18-19 -0.1630881020971202 1107 20-21 -0.21895388201065913 1107 22-23 -0.12347467347391472 1107 24-25 -0.1397305739612804 1107 26-27 -0.3411846167457995 1107 28-29 -0.28927398563967444 1107 30-31 -0.09686148228270497 1107 32-33 -0.0022094155487053513 1107 34-35 -0.13197442440480955 1107 36-37 -0.03866624392431817 1107 38-39 0.005098930924738454 1107 40-41 -0.013988765652641177 1107 42-43 0.0016119014102855544 1107 44-45 0.014240412652796408 1107 46-47 -0.0515053792355431 1107 48-49 -0.012781158063152276 1107 50-51 0.022698392840464265 1107 52-53 -0.05536153475459571 1107 54-55 0.021116995701365937 1107 56-57 -0.07416335441504529 1107 58-59 -0.07462916908353989 1107 60-61 -0.1821077213815414 1107 62-63 -0.21157876534136832 1107 64-65 -0.19813087536079266 1107 66-67 -0.14963240440625825 1107 68-69 -0.15344880080619916 1107 70-71 -0.12868968236751854 1107 72-73 -0.02662546650492814 1107 74-75 -0.268586589595305 1107 76-77 -0.1581307999050665 1108 1 0.40576474038655874 1108 2 0.4907766131923452 1108 3 0.5366619873554228 1108 4 0.4039268643478664 1108 5 0.49280897856937855 1108 6 0.47608644231350183 1108 7 0.5240912637404662 1108 8 0.4429324749191039 1108 9 0.4986316374148956 1108 10-11 0.5521691368862065 1108 12-13 0.6054221523800365 1108 14-15 0.6652512369332158 1108 16-17 0.6026481394597099 1108 18-19 0.6127027519722361 1108 20-21 0.569100027597635 1108 22-23 0.5353448757525285 1108 24-25 0.6263401119891583 1108 26-27 0.5387391417732843 1108 28-29 0.6868897598898869 1108 30-31 0.6945952638032296 1108 32-33 0.7207394310248674 1108 34-35 0.7257893878215853 1108 36-37 0.7411867267934014 1108 38-39 0.798484884413007 1108 40-41 0.8192050986898209 1108 42-43 0.7469931666526506 1108 44-45 0.7336129799393021 1108 46-47 0.7045641668874154 1108 48-49 0.7064073863319997 1108 50-51 0.5977298297905129 1108 52-53 0.7291115108644597 1108 54-55 0.7857331319908774 1108 56-57 0.8354359468481327 1108 58-59 0.7926046628584906 1108 60-61 0.7272456282441802 1108 62-63 0.8127632523112887 1108 64-65 0.8217043095338994 1108 66-67 0.8873426895166538 1108 68-69 0.8325788608884857 1108 70-71 0.8418357611777267 1108 72-73 0.8823080302036814 1108 74-75 0.9150780601785904 1108 76-77 0.9151323174215555 1109 1 -0.03037747704743765 1109 2 -0.18394101686249087 1109 3 -0.1560359075872384 1109 4 0.043855069334128416 1109 5 -0.10306153064932033 1109 6 -0.2216966488819878 1109 7 -0.08421221710408133 1109 8 0.023427311192619982 1109 9 -0.099494850552027 1109 10-11 -0.1720086643595664 1109 12-13 -0.11063201041423554 1109 14-15 -0.04715607840807223 1109 16-17 -0.07369878886557757 1109 18-19 -0.13409179157833506 1109 20-21 -0.013051182665726913 1109 22-23 -0.09361155930850629 1109 24-25 -0.11783560978458496 1109 26-27 -0.20642276783497948 1109 28-29 -0.0712373384340097 1109 30-31 0.08355802244643229 1109 32-33 0.08367411963457982 1109 34-35 0.048927985341897795 1109 36-37 -0.008227951511209142 1109 38-39 0.10272132960880498 1109 40-41 0.14224107107187933 1109 42-43 0.15182421309904726 1109 44-45 0.12010528323136072 1109 46-47 0.045478346792968694 1109 48-49 0.26315873293208014 1109 50-51 0.204593400456897 1109 52-53 0.19957477535875157 1109 54-55 0.25686793110358863 1109 56-57 0.24334904762835663 1109 58-59 0.23758648167260432 1109 60-61 0.33794004060348115 1109 62-63 0.20779803378752248 1109 64-65 0.3281580538787807 1109 66-67 0.26843624340177286 1109 68-69 0.22016576135026256 1109 70-71 0.15203046150487154 1109 72-73 0.023684910378182877 1109 74-75 0.08335596280749158 1109 76-77 0.301278101185563 1110 1 -0.4244732935116744 1110 2 -0.3972844716533075 1110 3 -0.573023087074418 1110 4 -0.41657003188044683 1110 5 -0.4681425023092416 1110 6 -0.4169058256701135 1110 7 -0.5283417717599548 1110 8 -0.6919573041919946 1110 9 -0.554589317488599 1110 10-11 -0.5447819437117971 1110 12-13 -0.47726493888927024 1110 14-15 -0.41006093670766575 1110 16-17 -0.5094781410922948 1110 18-19 -0.5462965824015527 1110 20-21 -0.6500704134349533 1110 22-23 -0.5110879425743633 1110 24-25 -0.5907522764512478 1110 26-27 -0.6414514452978679 1110 28-29 -0.40151736542456007 1110 30-31 -0.2900081044091678 1110 32-33 -0.3486048952102294 1110 34-35 -0.5052639849684866 1110 36-37 -0.3451797058971735 1110 38-39 -0.48686200372453214 1110 40-41 -0.47855008952190303 1110 42-43 -0.40898044277140144 1110 44-45 -0.4538150946363828 1110 46-47 -0.48256652500190356 1110 48-49 -0.5769812805631993 1110 50-51 -0.5873263026470177 1110 52-53 -0.5749360208625696 1110 54-55 -0.5299404359679336 1110 56-57 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-0.4538810579538932 2314 32-33 -0.5258449141233186 2314 34-35 -0.6116057799387917 2314 36-37 -1.1413039080012553 2314 38-39 -0.6884932576031915 2314 40-41 -0.5398832590895637 2314 42-43 -0.44385699054172534 2314 44-45 -0.37273446282102896 2314 46-47 -0.2326136230248963 2314 48-49 -0.17386976352664263 2314 50-51 -0.15637846878667716 2314 52-53 -0.08596296093172029 2314 54-55 -0.11127087200327779 2314 56-57 -0.10952457821756312 2314 58-59 -0.21012578573088803 2314 60-61 -0.14756198738198023 2314 62-63 -0.22543768658443852 2314 64-65 -0.1797559223517986 2314 66-67 -0.16389858106112243 2314 68-69 -0.13864418550592816 2314 70-71 -0.08733074154486431 2314 72-73 -0.22985325933723288 2314 74-75 -0.1355765805103779 2314 76-77 -0.11209527470222014 2315 1 -1.252376378967007 2315 2 -1.177852791844522 2315 3 -1.1103962569011259 2315 4 -0.9650608540563965 2315 5 -1.074576608536752 2315 6 -1.0597761193925024 2315 7 -1.3690847936283248 2315 8 -1.3807753420936635 2315 9 -0.8785394075669828 2315 10-11 -1.1167451768523478 2315 12-13 -1.0420767584160728 2315 14-15 -1.0632127265505105 2315 16-17 -0.9302517203918867 2315 18-19 -0.9608397500999075 2315 20-21 -0.8723513871196431 2315 22-23 -0.9344424464463685 2315 24-25 -1.0350955318265207 2315 26-27 -1.0074174441225594 2315 28-29 -1.0394941498758996 2315 30-31 -0.9761446859322191 2315 32-33 -0.9808732329650809 2315 34-35 -1.1123085511655049 2315 36-37 -1.125682250740958 2315 38-39 -1.0112198656413867 2315 40-41 -1.034158392884052 2315 42-43 -1.178378460905737 2315 44-45 -1.1149853554917009 2315 46-47 -0.9371350657980386 2315 48-49 -0.9271092231786078 2315 50-51 -0.8786577651940917 2315 52-53 -0.8598791226855127 2315 54-55 -1.1116041643747856 2315 56-57 -1.220319181401461 2315 58-59 -1.0440141730319574 2315 60-61 -1.2264571970555913 2315 62-63 -1.1814725149607526 2315 64-65 -1.077467583568211 2315 66-67 -1.155930312044223 2315 68-69 -1.3259171710330335 2315 70-71 -1.247619301692108 2315 72-73 -1.235297547645665 2315 74-75 -1.3913029589076622 2315 76-77 -1.3936233588093714 2316 1 -2.253114468722515 2316 2 -1.8133450831219164 2316 3 -1.9976632271941455 2316 4 -2.9405135250835173 2316 5 -2.4617894393573465 2316 6 -2.384220911485798 2316 7 -2.0265316370451387 2316 8 -1.6614613782698484 2316 9 -2.2181400879431195 2316 10-11 -2.0560978351648984 2316 12-13 -2.688225941958848 2316 14-15 -3.7983935986764763 2316 16-17 -2.9257934461637305 2316 18-19 -2.4209118013003774 2316 20-21 -2.15901432730314 2316 22-23 -2.6030942856943184 2316 24-25 -2.524806073145072 2316 26-27 -2.5336205093307456 2316 28-29 -3.0314910292392057 2316 30-31 -3.2060546937074434 2316 32-33 -3.1976867617395612 2316 34-35 -3.1020807610930667 2316 36-37 -3.0869115331644323 2316 38-39 -3.2249439875301995 2316 40-41 -2.915261724863896 2316 42-43 -3.186870858235558 2316 44-45 -3.1324521906704064 2316 46-47 -3.128995014482726 2316 48-49 -2.9530282905265857 2316 50-51 -2.9789376029866084 2316 52-53 -2.92739028309245 2316 54-55 -3.0927768405814824 2316 56-57 -2.9068136095474237 2316 58-59 -3.0306380906087114 2316 60-61 -2.8589506068638606 2316 62-63 -2.786016744390242 2316 64-65 -2.832950904333451 2316 66-67 -2.766520262375362 2316 68-69 -2.943559988793357 2316 70-71 -2.7907692815069893 2316 72-73 -2.8276964008732044 2316 74-75 -3.081391451164368 2316 76-77 -3.3520100552644685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93040.0 3 7815.0 4 9567.0 5 12009.0 6 13221.0 7 14273.0 8 16001.0 9 16297.0 10 18517.0 11 20057.0 12 17722.0 13 16410.0 14 16771.0 15 17334.0 16 18132.0 17 19207.0 18 20195.0 19 21498.0 20 23264.0 21 25194.0 22 27919.0 23 31614.0 24 36846.0 25 43233.0 26 51177.0 27 59798.0 28 71264.0 29 83397.0 30 100041.0 31 120146.0 32 146638.0 33 183687.0 34 243095.0 35 328095.0 36 465733.0 37 621913.0 38 536953.0 39 136417.0 40 938.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.272520394451508 26.15617587470046 27.612145531113967 22.959158199734055 2 23.00307709913301 26.44270228735264 27.67621392383984 22.878006689674514 3 23.22031862563175 26.549976720490694 27.173786597750322 23.055918056127233 4 23.047862341647438 26.51107725629118 27.44912689475122 22.991933507310158 5 23.049134566670634 26.67251836550823 27.420094485502606 22.85825258231853 6 23.223933612512027 26.56349740817266 27.281840474072954 22.93072850524236 7 23.343994355389654 26.261797956312034 27.290228038263077 23.10397965003524 8 23.059861695462793 26.5587197747308 27.288598904695437 23.092819625110973 9 23.093359303512614 26.36614713711704 27.33549415589989 23.204999403470453 10-11 23.349820339901278 26.41929993363718 27.281530742101552 22.949348984359997 12-13 23.368123361604535 26.383074692410506 27.193352900615402 23.05544904536956 14-15 23.143041598135337 26.47840095781025 27.34201717732067 23.036540266733745 16-17 23.39714541879228 26.3251369291067 27.309847074464493 22.967870577636525 18-19 23.24752095242067 26.374766649892045 27.117883757941797 23.25982863974549 20-21 23.241614852013466 26.417686428164068 27.158691973863657 23.18200674595881 22-23 23.333971231942364 26.410662389776924 26.975850454253088 23.279515924027624 24-25 23.334469301528024 26.501053751090698 26.9125126784173 23.251964268963977 26-27 23.367532076335415 26.61272386496629 26.890179847726476 23.12956421097181 28-29 23.43142269154212 26.504153031274246 26.838649168381636 23.225775108801997 30-31 23.282260001673354 26.453430783805153 26.887302199922807 23.37700701459868 32-33 23.248926772780006 26.653902153094002 26.832468593318303 23.264702480807685 34-35 23.276382915927407 26.478563532884493 26.977240120901275 23.26781343028683 36-37 23.302171932499423 26.473398776188645 27.03878704405821 23.18564224725373 38-39 23.202483917455037 26.522372505022545 27.031874655345117 23.2432689221773 40-41 23.140644429227578 26.467879619525537 27.016564042782733 23.374911908464156 42-43 23.06551087371809 26.490931471749306 26.978154584765175 23.46540306976743 44-45 23.113910810746976 26.44944176583519 27.062085626271585 23.37456179714624 46-47 23.055555780489037 26.25784826653214 27.08325067027928 23.603345282699546 48-49 23.06510618818375 26.391982362969145 26.924259338632933 23.61865211021417 50-51 23.002576771694194 26.49038998186366 26.875738218568166 23.63129502787397 52-53 23.114338580287907 26.30995690348692 26.99507645824627 23.580628057978902 54-55 22.967946968525183 26.373546133004655 27.011335080929577 23.647171817540585 56-57 23.024649587608025 26.468096270590248 26.91882989023304 23.58842425156869 58-59 23.093973683120193 26.435306980104368 26.944510087363486 23.52620924941195 60-61 23.056742756551824 26.341875287052808 26.851137164375128 23.750244792020244 62-63 22.98960258649057 26.253070944659157 27.09282465901413 23.664501809836146 64-65 22.9983578809903 26.208378653451614 27.024188020264724 23.769075445293357 66-67 22.931452896700826 26.281952394429574 27.03733650878357 23.749258200086025 68-69 22.92431544347428 26.10425840699555 27.150948520312628 23.820477629217535 70-71 22.86763884877847 26.072615682394044 27.227836331117615 23.831909137709868 72-73 22.932370980837703 25.883837957606133 27.258865790742615 23.92492527081355 74-75 22.789213301790248 25.879802800794828 27.192406971709804 24.13857692570512 76-77 22.874651138012215 25.754552676792862 27.311428844983404 24.05936734021152 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5107.0 1 3139.0 2 1089.0 3 1055.0 4 1103.0 5 1185.5 6 1334.5 7 1482.0 8 1587.5 9 1612.0 10 1643.0 11 1792.5 12 2024.5 13 2138.0 14 2288.0 15 2610.0 16 2931.0 17 3080.0 18 3408.5 19 4127.0 20 5096.0 21 6643.0 22 7611.0 23 9024.5 24 12164.5 25 16870.5 26 19850.0 27 23318.5 28 32352.5 29 42895.0 30 47872.0 31 54550.0 32 68011.0 33 80873.5 34 93844.5 35 100736.0 36 107111.5 37 119447.0 38 129494.5 39 133582.0 40 138489.0 41 148547.0 42 159070.0 43 164442.0 44 167836.0 45 175009.5 46 181404.0 47 184775.5 48 185532.0 49 186221.0 50 185085.5 51 180576.5 52 177892.0 53 175263.0 54 165479.0 55 150360.5 56 134913.0 57 127429.0 58 119590.0 59 101498.5 60 82441.0 61 73636.0 62 64898.0 63 48486.0 64 35255.0 65 29698.0 66 25000.0 67 17646.0 68 12779.5 69 8953.5 70 7338.0 71 6227.5 72 4209.0 73 2822.5 74 2344.0 75 2047.5 76 1424.5 77 905.0 78 712.0 79 573.5 80 375.5 81 257.5 82 173.5 83 148.0 84 130.0 85 100.5 86 93.0 87 97.0 88 87.0 89 64.0 90 47.5 91 44.0 92 46.5 93 43.0 94 34.5 95 34.5 96 37.0 97 33.5 98 38.0 99 79.5 100 113.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0035353540805542574 2 0.00850104225476787 3 0.012225308385428079 4 0.019430953725183702 5 0.014060454015028763 6 0.013844554529193387 7 0.017676770402771283 8 0.019026142189242378 9 0.017973632195794925 10-11 0.021414530251296207 12-13 0.02090176897243719 14-15 0.008366105076120761 16-17 0.006099160474849329 18-19 0.011510141338598403 20-21 0.010660037113121615 22-23 0.014978026829829105 24-25 0.021738379480049266 26-27 0.013925516836381654 28-29 0.00817719302601481 30-31 0.0080422558473677 32-33 0.00983692032337425 34-35 0.011375204159951293 36-37 0.009809932887644828 38-39 0.01713702168818285 40-41 0.012819031971475358 42-43 0.014964533111964395 44-45 0.017960138477930217 46-47 0.016975097073806317 48-49 0.013385768121793219 50-51 0.026623105347074613 52-53 0.03195312390363542 54-55 0.02458555394950327 56-57 0.01562572528733523 58-59 0.016718716434376814 60-61 0.02030804538638991 62-63 0.025611076507221296 64-65 0.020604907179413552 66-67 0.020415995129307602 68-69 0.030549777245705487 70-71 0.05692999567121531 72-73 0.06902036687799629 74-75 0.08039557103794757 76-77 0.07700864785390514 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3705428.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.13180916855364 #Duplication Level Percentage of deduplicated Percentage of total 1 72.97178298392305 40.23066414158756 2 13.814366627282592 15.2322204935956 3 5.410647983135682 8.948964362533681 4 2.6502195484156643 5.844455935920913 5 1.496182439174938 4.12436223589669 6 0.9232644737329888 3.054074446677135 7 0.5943871043746173 2.29387454874414 8 0.4245393119251422 1.8724496263684811 9 0.2980070706762085 1.4786702056260366 >10 1.3390747216787902 12.588900318723647 >50 0.05897870407629333 2.18625493133913 >100 0.017713666725484262 1.6787323629074098 >500 7.370866602312925E-4 0.28283296882676584 >1k 4.913910916091271E-5 0.03738596624781588 >5k 4.913910916091271E-5 0.14615745500504831 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5395 0.14559721576023066 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.6192461437653085E-4 1.0794974291768724E-4 3 0.0 0.0 0.0 4.3179897167074896E-4 1.6192461437653085E-4 4 8.096230718826543E-5 0.0 0.0 0.0013223843507416688 1.6192461437653085E-4 5 2.1589948583537448E-4 0.0 0.0 0.0026177812657539154 1.6192461437653085E-4 6 2.698743572942181E-4 0.0 0.0 0.005154600224319566 1.6192461437653085E-4 7 2.698743572942181E-4 0.0 0.0 0.011766521978027909 1.6192461437653085E-4 8 2.698743572942181E-4 0.0 0.0 0.018567355781842206 2.698743572942181E-4 9 2.698743572942181E-4 0.0 0.0 0.025719026250138985 2.698743572942181E-4 10 2.968617930236399E-4 0.0 0.0 0.03416609363344801 2.968617930236399E-4 11 4.8577384312959256E-4 0.0 0.0 0.04347675896009854 2.968617930236399E-4 12 4.8577384312959256E-4 0.0 0.0 0.0579150370753392 2.968617930236399E-4 13 4.8577384312959256E-4 0.0 0.0 0.07761586515781713 3.238492287530617E-4 14 5.937235860472798E-4 0.0 0.0 0.10104095937095525 3.508366644824835E-4 15 5.937235860472798E-4 0.0 0.0 0.12659806100671772 3.508366644824835E-4 16 5.937235860472798E-4 0.0 0.0 0.15334260981457473 3.508366644824835E-4 17 6.207110217767017E-4 0.0 0.0 0.17968234708649042 3.508366644824835E-4 18 6.476984575061234E-4 0.0 0.0 0.20648087076580626 3.508366644824835E-4 19 6.476984575061234E-4 0.0 0.0 0.2347097285387815 3.508366644824835E-4 20 7.286607646943889E-4 0.0 0.0 0.26879485986504126 3.508366644824835E-4 21 7.286607646943889E-4 0.0 0.0 0.302178317862336 4.0481153594132716E-4 22 9.445602505297634E-4 0.0 0.0 0.33982579070487945 4.5878640740017076E-4 23 9.98535121988607E-4 0.0 0.0 0.37979418302015316 4.8577384312959256E-4 24 0.0011064848649062942 0.0 0.0 0.42313600480160457 4.8577384312959256E-4 25 0.0011064848649062942 0.0 0.0 0.4656412160754439 4.8577384312959256E-4 26 0.0011064848649062942 0.0 0.0 0.5139757134668383 5.127612788590144E-4 27 0.0011064848649062942 0.0 0.0 0.5597464044639378 5.127612788590144E-4 28 0.0011064848649062942 2.698743572942181E-5 0.0 0.6075681405764732 5.667361503178581E-4 29 0.0011334723006357161 2.698743572942181E-5 0.0 0.6567662359112092 6.207110217767017E-4 30 0.0011604597363651379 2.698743572942181E-5 0.0 0.7115507304419354 6.207110217767017E-4 31 0.0011604597363651379 2.698743572942181E-5 0.0 0.7715438000684401 6.746858932355453E-4 32 0.0012144346078239814 2.698743572942181E-5 0.0 0.8357199222330052 6.746858932355453E-4 33 0.0012144346078239814 2.698743572942181E-5 0.0 0.900516755419347 7.01673328964967E-4 34 0.0012144346078239814 2.698743572942181E-5 0.0 0.9701713270369846 7.01673328964967E-4 35 0.0012144346078239814 2.698743572942181E-5 0.0 1.043954976321224 7.01673328964967E-4 36 0.0012144346078239814 2.698743572942181E-5 0.0 1.120734230971429 7.01673328964967E-4 37 0.0012144346078239814 2.698743572942181E-5 0.0 1.2011837768808353 7.01673328964967E-4 38 0.0012144346078239814 2.698743572942181E-5 0.0 1.2831176317553599 8.096230718826543E-4 39 0.0012144346078239814 2.698743572942181E-5 0.0 1.3678042050742856 8.096230718826543E-4 40 0.0012144346078239814 2.698743572942181E-5 0.0 1.456565881188354 8.096230718826543E-4 41 0.0012144346078239814 2.698743572942181E-5 0.0 1.5511838308557069 8.366105076120761E-4 42 0.0012144346078239814 2.698743572942181E-5 0.0 1.65184696612645 8.366105076120761E-4 43 0.0012144346078239814 2.698743572942181E-5 0.0 1.7600126085299728 9.175728148003415E-4 44 0.001268409479282825 2.698743572942181E-5 0.0 1.8759506324235689 9.175728148003415E-4 45 0.001268409479282825 2.698743572942181E-5 0.0 2.0000388619074503 9.175728148003415E-4 46 0.001268409479282825 2.698743572942181E-5 0.0 2.1324392215959938 9.445602505297634E-4 47 0.0013223843507416688 2.698743572942181E-5 0.0 2.265595229484961 9.715476862591851E-4 48 0.0013223843507416688 2.698743572942181E-5 0.0 2.3986432876310104 9.98535121988607E-4 49 0.0013493717864710905 2.698743572942181E-5 0.0 2.536791971129921 0.0011064848649062942 50 0.0013493717864710905 2.698743572942181E-5 0.0 2.682874960733281 0.0011064848649062942 51 0.0013763592222005123 2.698743572942181E-5 0.0 2.8321154803169835 0.0011064848649062942 52 0.001403346657929934 2.698743572942181E-5 0.0 2.9940940695649734 0.0011064848649062942 53 0.001403346657929934 2.698743572942181E-5 0.0 3.165167424653778 0.0011064848649062942 54 0.001403346657929934 2.698743572942181E-5 0.0 3.3524332411802362 0.0011334723006357161 55 0.001403346657929934 2.698743572942181E-5 0.0 3.5432613992229776 0.0011334723006357161 56 0.001700208450953574 2.698743572942181E-5 0.0 3.744047921049876 0.0011334723006357161 57 0.001700208450953574 2.698743572942181E-5 0.0 3.9507986661729766 0.0011874471720945596 58 0.001700208450953574 2.698743572942181E-5 0.0 4.165456729964798 0.001268409479282825 59 0.001700208450953574 2.698743572942181E-5 0.0 4.378333622998476 0.001268409479282825 60 0.001700208450953574 2.698743572942181E-5 0.0 4.596473065999393 0.001268409479282825 61 0.0017541833224124176 5.397487145884362E-5 0.0 4.825002671756137 0.001268409479282825 62 0.0017541833224124176 5.397487145884362E-5 0.0 5.0695088394646985 0.001268409479282825 63 0.0017811707581418396 5.397487145884362E-5 0.0 5.318413959197156 0.0013493717864710905 64 0.0017811707581418396 5.397487145884362E-5 0.0 5.577466354763876 0.0013493717864710905 65 0.0017811707581418396 5.397487145884362E-5 0.0 5.851820626389178 0.0013493717864710905 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCGCT 355 0.0 17.997423 37 ATTCGCG 375 0.0 17.039402 56 CGCGGAA 385 0.0 16.59906 59 CGGTTAC 325 8.0035534E-11 16.381828 4 CGATCGT 445 0.0 15.9525585 1 CAATTCG 490 0.0 15.93929 54 CTCGCGC 425 1.8189894E-12 15.032737 36 AATTCGC 455 0.0 14.824255 55 TAACGCG 170 7.341509E-4 14.619304 52 CGCGCTT 480 0.0 14.050642 38 GCGCGAG 430 3.8198777E-10 13.208482 24 ACGATCG 350 6.1369064E-8 13.187031 59 AGCGCGA 410 2.6502676E-9 12.987349 23 TAAGGCG 770 0.0 12.907592 17 TCGCGGA 505 2.910383E-11 12.654216 58 CCTCGCG 540 7.2759576E-12 12.488447 35 CGCTCCG 600 0.0 12.423054 25 ATCGTTC 600 0.0 12.422887 3 GACGCAT 315 3.5928006E-6 12.398064 48 AACGCGA 260 1.0309888E-4 12.289356 53 >>END_MODULE