FastQCFastQC Report
Thu 26 May 2016
SRR936136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936136_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3705428
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64500.17406896045477066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45060.12160538539677467No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14350.038.36595100-101
CGTACAT18600.035.51945100-101
GGTATCA81300.034.4726871
CGGTATC12250.031.338615100-101
GTATCAA151550.030.4292011
GCGTATC5050.027.696995100-101
CCGTACA9350.027.054127100-101
ATACACG10650.026.54194696-97
TCGTACA10400.026.325745100-101
TTATACG10400.026.32165796-97
CCTGTCG14150.025.86469896-97
AGTATCA35300.025.375696100-101
TACGCAG11000.025.160336100-101
TCAACGC189450.024.086914
ATCAACG191150.023.8415663
TATCAAC193450.023.6199342
CAACGCA196400.023.264845
ACCGTAC3900.022.126598-99
AACGCAG207350.022.0649346
AGGTATC26400.021.86875100-101
Produced by FastQC (version 0.11.5)