FastQCFastQC Report
Thu 26 May 2016
SRR936135_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936135_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3616451
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64030.1770520325036894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC3900.025.4795464
CGATCGT5200.019.7946111
CGCGGAA5000.018.46059659
TCGCGGA5050.018.27706158
CTCGCGC5200.017.7476336
CCTCGCG5600.017.74713735
CGCTCCG5850.017.59666825
TCGCGCT5500.016.78050437
GGTAACG2752.561137E-916.778881
TAGGCCG6350.016.766633
TTAGTCG2803.250534E-916.48062515
TTGCGTA5400.016.4329936
ATTCGCG6300.016.34157456
ATCGTTC6100.016.2902013
CGCGACT1604.6359652E-415.53218254
CGCGCTT6200.015.45761138
GCGTAAG6000.015.38127738
ACCGCTA2553.3302967E-715.31252637
GCGGTTA6500.015.2877273
CGTAAGT6050.015.25479339
Produced by FastQC (version 0.11.5)