##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936135_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3616451 Sequences flagged as poor quality 0 Sequence length 77 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.47440902697147 39.0 36.0 41.0 30.0 41.0 2 36.3299729486173 39.0 36.0 41.0 29.0 41.0 3 36.23544076775823 39.0 36.0 41.0 29.0 41.0 4 36.12102113370263 39.0 36.0 41.0 29.0 41.0 5 36.04735222459809 39.0 35.0 41.0 28.0 41.0 6 35.959136733775736 39.0 35.0 40.0 28.0 41.0 7 35.79411389785179 39.0 35.0 40.0 27.0 41.0 8 35.62276579995139 39.0 35.0 40.0 27.0 41.0 9 35.543662281059525 38.0 35.0 40.0 27.0 41.0 10-11 35.757965751506106 39.0 35.0 40.0 27.5 41.0 12-13 35.96372175373038 39.0 35.0 41.0 28.5 41.0 14-15 35.917099388322974 39.0 35.0 41.0 28.0 41.0 16-17 35.76056774998472 39.0 35.0 41.0 27.0 41.0 18-19 35.62021440356858 39.0 35.0 41.0 27.0 41.0 20-21 35.437090396081686 39.0 35.0 41.0 26.5 41.0 22-23 35.19819375404229 39.0 35.0 41.0 26.0 41.0 24-25 34.98238134568946 39.0 34.0 41.0 24.5 41.0 26-27 34.04765127468892 38.0 33.0 39.5 23.5 40.5 28-29 34.08150324724433 38.0 33.0 39.5 23.5 40.5 30-31 34.45430865785268 38.0 34.0 40.0 23.5 41.0 32-33 34.292015431703625 38.0 34.0 40.0 23.0 41.0 34-35 34.00772981577796 38.0 33.0 40.0 22.5 41.0 36-37 33.71105539657526 37.5 33.0 40.0 22.0 41.0 38-39 33.42571446426344 37.0 33.0 40.0 21.0 41.0 40-41 33.04049107813157 36.5 32.0 39.5 19.5 41.0 42-43 32.67713581630167 36.0 32.0 39.0 18.5 41.0 44-45 32.304805595319834 35.5 32.0 39.0 17.5 40.5 46-47 31.914689567202764 35.0 31.0 38.5 14.0 40.0 48-49 31.4457863524212 35.0 31.0 37.5 9.5 40.0 50-51 31.011604885563223 35.0 31.0 37.0 7.5 39.0 52-53 30.558941348852784 35.0 30.0 36.5 4.5 39.0 54-55 30.103041075352603 34.0 30.0 36.0 2.0 38.5 56-57 29.66567499462871 34.0 29.5 35.5 2.0 37.0 58-59 29.24631192293218 34.0 29.0 35.0 2.0 37.0 60-61 28.904929999051557 34.0 29.0 35.0 2.0 36.0 62-63 28.562566311557934 34.0 29.0 35.0 2.0 36.0 64-65 28.202095507446387 34.0 28.5 35.0 2.0 35.5 66-67 27.826043543794732 34.0 27.0 35.0 2.0 35.0 68-69 27.4753594062245 33.5 27.0 35.0 2.0 35.0 70-71 27.05871419245 33.0 26.0 35.0 2.0 35.0 72-73 26.579965137091584 33.0 25.0 35.0 2.0 35.0 74-75 26.000133003322873 33.0 24.5 35.0 2.0 35.0 76-77 24.28570814314918 31.0 11.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7318431276117678 1101 2 0.7176046026133847 1101 3 0.9606848342715608 1101 4 0.8656443561112042 1101 5 0.8917992379330428 1101 6 0.8338761816938742 1101 7 0.7772318085113099 1101 8 0.9453516703060885 1101 9 1.024689919652161 1101 10-11 0.9384592709736737 1101 12-13 0.9279717011434698 1101 14-15 0.8895100939032119 1101 16-17 0.9965714636842549 1101 18-19 0.9824783817515268 1101 20-21 0.9475136396637112 1101 22-23 0.9742935579548728 1101 24-25 1.0466887266461455 1101 26-27 0.754535991962122 1101 28-29 0.9068158680293763 1101 30-31 0.9562622449743188 1101 32-33 1.043110361133671 1101 34-35 0.983781032829107 1101 36-37 0.9508980787389589 1101 38-39 0.9463679069149222 1101 40-41 0.9065813716706685 1101 42-43 0.8527206160934924 1101 44-45 0.6430412183019101 1101 46-47 0.6960861151720543 1101 48-49 0.7791103907250907 1101 50-51 0.8337308915763337 1101 52-53 0.9911020461545696 1101 54-55 0.9503614372603515 1101 56-57 0.8254583011121177 1101 58-59 0.8631934421454908 1101 60-61 0.855683807899684 1101 62-63 0.68429022776143 1101 64-65 0.6735049655446979 1101 66-67 0.6583893512121399 1101 68-69 0.7900900593685058 1101 70-71 0.7223221418451651 1101 72-73 0.6206254385392249 1101 74-75 0.5110206368486168 1101 76-77 0.6617873427060523 1102 1 0.839624210194053 1102 2 0.9208518119448783 1102 3 0.8475582104877191 1102 4 0.9446388195682331 1102 5 1.0008639384401121 1102 6 0.9271914779616353 1102 7 0.8686906607089 1102 8 0.9026262219177781 1102 9 0.939626580883818 1102 10-11 0.8747887579923699 1102 12-13 0.9489448963917155 1102 14-15 0.8735849178626438 1102 16-17 0.8960384146437477 1102 18-19 0.9564619154635139 1102 20-21 0.8917456906930781 1102 22-23 0.904627183942587 1102 24-25 0.8257235943245504 1102 26-27 0.7937950090017765 1102 28-29 0.8764843921554473 1102 30-31 0.9302625316561546 1102 32-33 0.8970749917620395 1102 34-35 0.9976462206952945 1102 36-37 0.920244085798501 1102 38-39 1.0977868924195704 1102 40-41 1.0809012190125955 1102 42-43 0.9724102830050327 1102 44-45 0.943300934128537 1102 46-47 0.8302111084529535 1102 48-49 0.8456216519460007 1102 50-51 0.9723020513835365 1102 52-53 0.9886500205682118 1102 54-55 0.9560465172625037 1102 56-57 1.0795631370763559 1102 58-59 1.0367310064249367 1102 60-61 0.9610427873123477 1102 62-63 0.960348979620381 1102 64-65 0.8966800564765904 1102 66-67 0.920294083254209 1102 68-69 1.0571225440041516 1102 70-71 1.0362330196291119 1102 72-73 1.190396988950436 1102 74-75 1.2348406723254932 1102 76-77 1.331500392104914 1103 1 0.6629610989063721 1103 2 0.48977146613749056 1103 3 0.4745215857658849 1103 4 0.5374558024765008 1103 5 0.6809287701281832 1103 6 0.6128358796071822 1103 7 0.6535369733346741 1103 8 0.8069447838749326 1103 9 0.5385449251999646 1103 10-11 0.5784455804335735 1103 12-13 0.611497507364156 1103 14-15 0.6852588680182379 1103 16-17 0.7454945640099666 1103 18-19 0.8106555713431334 1103 20-21 0.7630368330492985 1103 22-23 0.6299057845914788 1103 24-25 0.5425157068530169 1103 26-27 0.3991761902512536 1103 28-29 0.5004758952314958 1103 30-31 0.7160405119130075 1103 32-33 0.6911876008344464 1103 34-35 0.6912614365658172 1103 36-37 0.6980845385263592 1103 38-39 0.6762738627472658 1103 40-41 0.6955447382908062 1103 42-43 0.6994313072420653 1103 44-45 0.6940256844603212 1103 46-47 0.45608915751430246 1103 48-49 0.5032080678073108 1103 50-51 0.4646439521726329 1103 52-53 0.5431207297151808 1103 54-55 0.5622085864067081 1103 56-57 0.539078777865047 1103 58-59 0.5420700125309743 1103 60-61 0.60386502412074 1103 62-63 0.47768199196201167 1103 64-65 0.542221186597704 1103 66-67 0.564453365672211 1103 68-69 0.38723786351325273 1103 70-71 0.5611230115971217 1103 72-73 0.7318648350816943 1103 74-75 0.6284060121304798 1103 76-77 0.798324351904661 1104 1 0.5322081645969305 1104 2 0.5932451681143291 1104 3 0.5473299080230376 1104 4 0.5941785181456112 1104 5 0.6193617187839209 1104 6 0.5967045617875399 1104 7 0.8083746281375142 1104 8 0.958998976298119 1104 9 0.7598131305770082 1104 10-11 0.5987722223458505 1104 12-13 0.7889368169135906 1104 14-15 0.8027678520333765 1104 16-17 0.864974317450617 1104 18-19 0.7205398340209683 1104 20-21 0.7542768908564241 1104 22-23 0.7850739667612814 1104 24-25 0.8071049738558358 1104 26-27 0.5456297898349263 1104 28-29 0.6677293358807361 1104 30-31 0.8465174207085511 1104 32-33 0.750040811521032 1104 34-35 0.8693638226921081 1104 36-37 0.8587564408871629 1104 38-39 0.79199052695256 1104 40-41 0.7915435111200466 1104 42-43 0.7750999219104528 1104 44-45 0.7624787812204588 1104 46-47 0.5683691282591177 1104 48-49 0.770053184089651 1104 50-51 0.6239370455571631 1104 52-53 0.5942009212804074 1104 54-55 0.6468984005378076 1104 56-57 0.7122351208258699 1104 58-59 0.7720134185100029 1104 60-61 0.5264011887374345 1104 62-63 0.4883686625668737 1104 64-65 0.4979239264586006 1104 66-67 0.6041209571122543 1104 68-69 0.45958933717303196 1104 70-71 0.4282839749085845 1104 72-73 0.4635102811881495 1104 74-75 0.2601229259894211 1104 76-77 0.26268286509411354 1105 1 0.04071638959988633 1105 2 0.3030762296911007 1105 3 0.20674412898384986 1105 4 0.2033791959739375 1105 5 0.1838491940094329 1105 6 -0.09656630001183686 1105 7 0.07700712733614523 1105 8 0.19455044304142888 1105 9 -0.11422839108467286 1105 10-11 0.056558958099081735 1105 12-13 0.16366182114364136 1105 14-15 -0.09458826036777168 1105 16-17 -0.049711906360265345 1105 18-19 0.1218746592795128 1105 20-21 0.11466453349839156 1105 22-23 -0.040361133216698875 1105 24-25 -0.041914632320121825 1105 26-27 -0.1633998579273097 1105 28-29 0.039420862771528675 1105 30-31 0.007355531801991333 1105 32-33 0.04811012312395491 1105 34-35 0.08871190182422595 1105 36-37 0.061514220990879664 1105 38-39 0.01692333505113197 1105 40-41 0.03565196535345905 1105 42-43 -0.13145822873715218 1105 44-45 -0.21447097428690398 1105 46-47 -0.2161274617678295 1105 48-49 -0.21679722688416447 1105 50-51 -0.04361183809222524 1105 52-53 -0.02769011943095734 1105 54-55 -0.20908616381804634 1105 56-57 -0.005500012567381418 1105 58-59 0.04172362394164608 1105 60-61 -0.20804731901142404 1105 62-63 -0.2380213659083772 1105 64-65 -0.05080837641468605 1105 66-67 -0.05976551089368698 1105 68-69 -0.3990625394812568 1105 70-71 -0.6051585036371954 1105 72-73 -0.9150032749533281 1105 74-75 -0.9732431990891222 1105 76-77 -0.722936154911686 1106 1 0.24174531654984577 1106 2 0.2565257063561006 1106 3 -0.038726549606238336 1106 4 0.10341639859515084 1106 5 0.012858536982925273 1106 6 0.1485263190492958 1106 7 0.21392687946369904 1106 8 0.3238262376802368 1106 9 0.22709197735893838 1106 10-11 0.025575156959924072 1106 12-13 0.04874272877202657 1106 14-15 0.1982877180917626 1106 16-17 0.3524318160840423 1106 18-19 0.30297443606378494 1106 20-21 0.1756099984325843 1106 22-23 0.3263998203124814 1106 24-25 0.3876725239550254 1106 26-27 0.30743287915570505 1106 28-29 0.24348705488525013 1106 30-31 0.3463254984607005 1106 32-33 0.3064223766714633 1106 34-35 0.35110671391954895 1106 36-37 0.3528393396092895 1106 38-39 0.2921452336458614 1106 40-41 0.32841458543770585 1106 42-43 0.38268072177843493 1106 44-45 0.23238514929174414 1106 46-47 0.40409253675698054 1106 48-49 0.26727044041801307 1106 50-51 0.21528318287482406 1106 52-53 0.18553915136707744 1106 54-55 0.2614783031069692 1106 56-57 0.09183850443314512 1106 58-59 7.458034340110942E-4 1106 60-61 -0.026618514019439488 1106 62-63 -0.05534311198765707 1106 64-65 0.03736124102544025 1106 66-67 0.07894218493333227 1106 68-69 -0.09709957860808416 1106 70-71 -0.17386068960135503 1106 72-73 -0.04810398001863092 1106 74-75 -0.11065186232025681 1106 76-77 -0.11472041743812866 1107 1 0.3345554500167154 1107 2 0.2297585429541158 1107 3 0.2105436026643943 1107 4 0.22458248020080163 1107 5 0.18547104861091412 1107 6 0.045075067040826866 1107 7 0.014744527800296225 1107 8 0.23606078386136886 1107 9 -9.466781227516208E-4 1107 10-11 0.15216000352028658 1107 12-13 0.30820434274451003 1107 14-15 0.2310879190631212 1107 16-17 0.25381776400725187 1107 18-19 0.227662573944734 1107 20-21 0.13554568593870187 1107 22-23 0.24384009683050323 1107 24-25 -0.05830377900101524 1107 26-27 -0.09573971577922435 1107 28-29 0.04802171995785187 1107 30-31 0.22786751424325047 1107 32-33 0.29830637540460714 1107 34-35 0.3509540784918741 1107 36-37 0.24310669618918723 1107 38-39 0.17246894345780817 1107 40-41 0.3018706959122639 1107 42-43 0.32477617985883 1107 44-45 0.30555742088165516 1107 46-47 0.15246159280510696 1107 48-49 0.38167409272429254 1107 50-51 0.23923090213175868 1107 52-53 0.314534037541339 1107 54-55 0.2809618299222194 1107 56-57 0.3796811050556279 1107 58-59 0.43507737467520613 1107 60-61 0.3336284552428843 1107 62-63 0.366859207473901 1107 64-65 0.526355986254444 1107 66-67 0.491285549147495 1107 68-69 0.37411231020766067 1107 70-71 0.4685669432975921 1107 72-73 0.4619569000435817 1107 74-75 0.34273293814271 1107 76-77 0.2952146897051442 1108 1 0.2044751422474107 1108 2 0.154557773530847 1108 3 0.11493554411667617 1108 4 0.05893878248184592 1108 5 -0.02598157513969568 1108 6 0.17239837935780145 1108 7 -0.06407695548899284 1108 8 0.18625364959434165 1108 9 0.02460283428035126 1108 10-11 -0.18695765438290124 1108 12-13 -0.07466230023069187 1108 14-15 0.06257116127847695 1108 16-17 -0.008267900228297265 1108 18-19 0.08844124629734296 1108 20-21 0.11455952504545053 1108 22-23 -0.010024866561487045 1108 24-25 0.06760279192705809 1108 26-27 -0.19128929094116387 1108 28-29 0.0317235260785651 1108 30-31 0.0748384984821513 1108 32-33 0.13970865088293039 1108 34-35 0.1771178429548499 1108 36-37 0.13370467802208452 1108 38-39 0.0915551984108518 1108 40-41 0.24753417835272984 1108 42-43 0.17303046452190074 1108 44-45 0.03006384228681469 1108 46-47 -0.04939281298251075 1108 48-49 0.009519989983377997 1108 50-51 0.24344786411795383 1108 52-53 0.14101512634430335 1108 54-55 0.18829315967986915 1108 56-57 0.07073789316847723 1108 58-59 0.016500368412661004 1108 60-61 -0.05832319535359787 1108 62-63 -0.12939848342473326 1108 64-65 -0.1467262411506134 1108 66-67 -0.15327702572182744 1108 68-69 0.0182657367221708 1108 70-71 -0.14790136623837924 1108 72-73 -0.055771528373298906 1108 74-75 -0.18807192033220232 1108 76-77 -0.2643831360151516 1109 1 0.011669986506845476 1109 2 -0.03914637575244484 1109 3 -0.09245559299053951 1109 4 0.06569382499832699 1109 5 -0.03789276742043768 1109 6 -0.023754394144305024 1109 7 -0.2550691723957357 1109 8 -0.03133442249890095 1109 9 0.13162335689274585 1109 10-11 -0.12119317621151282 1109 12-13 -0.047586179148602525 1109 14-15 0.1453574180091266 1109 16-17 0.2875577919746988 1109 18-19 0.13475979534191396 1109 20-21 0.14191823417130678 1109 22-23 0.21986888788863013 1109 24-25 0.11488006281433627 1109 26-27 0.05684205528907427 1109 28-29 0.1393990926553954 1109 30-31 0.3174805857336338 1109 32-33 0.3356856233018348 1109 34-35 0.3636272896268409 1109 36-37 0.4178422737683576 1109 38-39 0.3625319504882043 1109 40-41 0.32923761372939 1109 42-43 0.4288298542387494 1109 44-45 0.3627256847325242 1109 46-47 0.09272536500688133 1109 48-49 0.2515517311286395 1109 50-51 0.2679104195889366 1109 52-53 0.3259898940885648 1109 54-55 0.33204532768337813 1109 56-57 0.26580619275492623 1109 58-59 0.3550977401785964 1109 60-61 0.3120187054889243 1109 62-63 0.2646908071572973 1109 64-65 0.2419439849764835 1109 66-67 0.2995328956917831 1109 68-69 0.29157370244709924 1109 70-71 0.2756157193605375 1109 72-73 0.11978497570018476 1109 74-75 0.2810468960181467 1109 76-77 0.3099586880226042 1110 1 -0.6151368968349615 1110 2 -0.5414281431405996 1110 3 -0.5548574971746589 1110 4 -0.39168709010598235 1110 5 -0.17016341646736777 1110 6 -0.24914910137354696 1110 7 -0.41323460939914014 1110 8 -0.3121801747788311 1110 9 -0.4021833111764508 1110 10-11 -0.39247412776538226 1110 12-13 -0.3158251818982194 1110 14-15 -0.47145595408959196 1110 16-17 -0.5561052122035406 1110 18-19 -0.4108458912798696 1110 20-21 -0.5763068859410225 1110 22-23 -0.6498463835016395 1110 24-25 -0.6080705052118063 1110 26-27 -0.5235800013471916 1110 28-29 -0.47456971483217814 1110 30-31 -0.552830813440508 1110 32-33 -0.3484346884031311 1110 34-35 -0.2730880967385758 1110 36-37 -0.38210066675769383 1110 38-39 -0.404074466348078 1110 40-41 -0.34317633226654465 1110 42-43 -0.3957192964129348 1110 44-45 -0.45670233226422496 1110 46-47 -0.4946080433456963 1110 48-49 -0.4491555800674938 1110 50-51 -0.5099190698235638 1110 52-53 -0.42343049051553194 1110 54-55 -0.32981475612616507 1110 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2314 14-15 -0.6293262960463153 2314 16-17 -1.0831919925108835 2314 18-19 -0.8770246092946579 2314 20-21 -0.8383561523505634 2314 22-23 -0.6107605197385624 2314 24-25 -0.47147263177708965 2314 26-27 -0.5055791887827752 2314 28-29 -0.6366719412181041 2314 30-31 -0.7124858362247721 2314 32-33 -0.8337197973232762 2314 34-35 -0.7431969040340007 2314 36-37 -0.9228035121533651 2314 38-39 -0.7928678043850184 2314 40-41 -0.5609930180891709 2314 42-43 -0.46270742140786325 2314 44-45 -0.45469904016101026 2314 46-47 -0.5243399376656583 2314 48-49 -0.43447391181492634 2314 50-51 -0.31185205506958624 2314 52-53 -0.32224666100391985 2314 54-55 -0.41432535471528453 2314 56-57 -0.16709425734763883 2314 58-59 -0.3160803817217577 2314 60-61 -0.24058003644648096 2314 62-63 -0.22042143398163105 2314 64-65 -0.2536719294578127 2314 66-67 -0.40852500616079723 2314 68-69 -0.17009354836360302 2314 70-71 -0.1764185978547843 2314 72-73 -0.1440766687169166 2314 74-75 -0.0559069281974196 2314 76-77 -0.009592964512854962 2315 1 -1.5160130363573217 2315 2 -1.4197964674002463 2315 3 -1.4507726410515644 2315 4 -1.4484062114658869 2315 5 -1.4752572278895286 2315 6 -1.4547145398712118 2315 7 -1.4782015185334103 2315 8 -1.505459382941865 2315 9 -1.3456843715438964 2315 10-11 -1.3501887956334215 2315 12-13 -1.4583282799906883 2315 14-15 -1.649856512131727 2315 16-17 -1.4895447000712565 2315 18-19 -1.3378964789794026 2315 20-21 -1.492223840358072 2315 22-23 -1.591388584635304 2315 24-25 -1.6099657899433382 2315 26-27 -1.458235435897386 2315 28-29 -1.373289139961912 2315 30-31 -1.6468928714065711 2315 32-33 -1.7467080640312247 2315 34-35 -1.5422887850265212 2315 36-37 -1.7137577620190925 2315 38-39 -1.6219954278541167 2315 40-41 -1.5860960473396943 2315 42-43 -1.8691363523435953 2315 44-45 -1.8434747945268626 2315 46-47 -1.7630385453317636 2315 48-49 -1.7067375221075984 2315 50-51 -1.6851524997190381 2315 52-53 -1.731359519140824 2315 54-55 -1.708371169678717 2315 56-57 -1.9247931129361682 2315 58-59 -1.7087849796021963 2315 60-61 -1.721538926508405 2315 62-63 -1.9787434353088607 2315 64-65 -1.865113058098121 2315 66-67 -1.7436618998067956 2315 68-69 -1.8184193254982404 2315 70-71 -1.8819684846787865 2315 72-73 -3.2023749779331467 2315 74-75 -3.771589612643254 2315 76-77 -3.449885701564604 2316 1 -3.5531447945139405 2316 2 -3.594261628929864 2316 3 -3.084118640316909 2316 4 -3.05221394082853 2316 5 -3.7677245258845247 2316 6 -3.263330389949516 2316 7 -3.598155563311252 2316 8 -3.4934958925228514 2316 9 -3.1578654705991553 2316 10-11 -3.0649443699855183 2316 12-13 -3.129149685492486 2316 14-15 -3.178550727392249 2316 16-17 -4.562007826360695 2316 18-19 -4.367760056283384 2316 20-21 -3.655353695685271 2316 22-23 -3.8929502071424693 2316 24-25 -3.7899385869466684 2316 26-27 -3.705893612427502 2316 28-29 -3.7235784625684403 2316 30-31 -3.898506435737879 2316 32-33 -4.039295215484842 2316 34-35 -4.15829268219705 2316 36-37 -4.097956602837083 2316 38-39 -4.257316724589934 2316 40-41 -4.138397272088028 2316 42-43 -4.278336317886126 2316 44-45 -4.092530601057881 2316 46-47 -3.70960208370494 2316 48-49 -3.934970970955252 2316 50-51 -4.400753119237763 2316 52-53 -5.327419494054357 2316 54-55 -4.495409467060487 2316 56-57 -4.3952713304727205 2316 58-59 -4.5012935281477375 2316 60-61 -4.243673665185053 2316 62-63 -4.275638984697178 2316 64-65 -4.202322888752949 2316 66-67 -4.3465771346863455 2316 68-69 -4.321904629566241 2316 70-71 -4.049967055256147 2316 72-73 -3.9916304844183372 2316 74-75 -4.131880292587805 2316 76-77 -4.127376084530752 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93257.0 3 7859.0 4 9377.0 5 11074.0 6 12881.0 7 14168.0 8 15922.0 9 16185.0 10 18522.0 11 20558.0 12 17625.0 13 16073.0 14 16510.0 15 17215.0 16 17775.0 17 19044.0 18 19792.0 19 21000.0 20 22448.0 21 24156.0 22 27104.0 23 31177.0 24 36082.0 25 42019.0 26 49802.0 27 59377.0 28 69519.0 29 82159.0 30 97981.0 31 117618.0 32 143843.0 33 180502.0 34 239288.0 35 319978.0 36 451178.0 37 602405.0 38 522238.0 39 131871.0 40 869.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.979744137934656 26.203945754653716 27.919169024877426 22.897141082534198 2 23.03750840018916 26.48867944878166 27.66935196170343 22.804460189325745 3 23.000086836715447 26.692306543561333 27.31042355852435 22.997183061198868 4 22.865519264987398 26.541660835998847 27.59781832542483 22.995001573588926 5 23.123512361966135 26.65980098178925 27.55577838918754 22.660908267057074 6 23.265068976902985 26.465555241531156 27.43097746822715 22.838398313338708 7 23.114685413316334 26.33092132463664 27.555307271839784 22.999085990207234 8 23.183342773195513 26.389070188325253 27.435871623774293 22.991715414704945 9 23.013972232531668 26.22259108832524 27.517128582040474 23.24630809710262 10-11 23.340171429480144 26.41013780595986 27.346618184634124 22.90307257992587 12-13 23.37561269163401 26.401020268897767 27.26376310026565 22.959603939202573 14-15 23.105598821955596 26.46570777723623 27.445212984759397 22.98348041604878 16-17 23.296377232903627 26.30684878094644 27.363557740767142 23.033216245382793 18-19 23.16859862545075 26.198409288557855 27.405665158518712 23.227326927472692 20-21 23.214643538564744 26.384766130891364 27.32264603828713 23.077944292256767 22-23 23.331812643446252 26.57521046850478 26.92417630027628 23.168800587772687 24-25 23.3468920975429 26.50900886885612 27.025527951727756 23.118571081873228 26-27 23.260094180824307 26.543326995976873 27.010704729895302 23.185874093303514 28-29 23.38377130973969 26.51986258894973 26.891762872607178 23.204603228703398 30-31 23.263130910617388 26.4430769417963 26.915082897281707 23.378709250304606 32-33 23.158445345262592 26.803633919513466 26.906728541876173 23.13119219334777 34-35 23.39347359146604 26.522581150079077 26.91026969505651 23.173675563398373 36-37 23.317194648031244 26.503528364148476 27.154293252102953 23.024983735717335 38-39 23.15474220878249 26.48254130935789 27.16462501090645 23.198091470953162 40-41 23.150676085912572 26.499019723476152 27.0426345275529 23.307669663058377 42-43 23.03441564179159 26.665115197936025 26.924467671304896 23.376001488967496 44-45 22.981689560477548 26.4312203917621 27.165712104912377 23.421377942847975 46-47 23.0764550184064 26.36250238550278 27.132044839154883 23.428997756935935 48-49 23.115409996752973 26.42819449200053 26.972873763626314 23.483521747620184 50-51 23.02020413393556 26.598433000892225 26.90129930446417 23.480063560708043 52-53 23.134715635057958 26.524840352034655 26.92399830151439 23.416445711392996 54-55 23.027916292748852 26.534964617659266 27.018889989264082 23.418229100327803 56-57 22.914456982620028 26.51719038540946 27.06027829089311 23.508074341077403 58-59 23.107087066465645 26.57771264525009 26.987226191831 23.327974096453268 60-61 23.083907501581628 26.400035953937472 26.897223595462886 23.61883294901801 62-63 22.919052502093404 26.331173433438735 27.161800265383494 23.587973799084363 64-65 23.052722982899184 26.314720983468675 27.00757938774943 23.62497664588271 66-67 22.994141080135062 26.41788557508068 27.10341999666706 23.484553348117203 68-69 22.908145531207776 26.18045518994464 27.206497757827066 23.70490152102052 70-71 22.955404630738347 26.219671784633704 27.259800644965658 23.565122939662288 72-73 22.962644797902865 26.180468224166635 27.162005384533632 23.69488159339686 74-75 22.81443992426317 26.129192374342413 27.10028487797102 23.956082823423397 76-77 22.72314068857692 25.94038916797221 27.447203848974233 23.88926629447664 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6086.0 1 3743.5 2 1318.5 3 1246.5 4 1257.0 5 1325.0 6 1417.5 7 1547.5 8 1707.0 9 1761.0 10 1807.0 11 1964.5 12 2117.5 13 2159.0 14 2328.0 15 2661.0 16 2965.0 17 3105.0 18 3432.5 19 4251.0 20 5262.5 21 6737.5 22 7692.0 23 8982.5 24 12077.0 25 16599.0 26 19317.0 27 23134.0 28 31858.0 29 41164.5 30 45564.0 31 52451.0 32 65857.0 33 79348.0 34 92403.5 35 98487.0 36 105980.5 37 120160.0 38 130143.5 39 133441.0 40 138567.5 41 148719.0 42 157882.5 43 162021.0 44 166123.0 45 173299.5 46 177502.0 47 179294.5 48 179959.0 49 178820.0 50 177516.0 51 172333.5 52 167316.0 53 165407.0 54 157745.5 55 144845.0 56 130200.0 57 122703.0 58 114644.5 59 97195.0 60 79180.0 61 70556.0 62 62817.0 63 46874.0 64 33237.5 65 27805.0 66 23723.5 67 17830.0 68 13453.0 69 9300.5 70 7713.0 71 6579.5 72 4578.0 73 3096.0 74 2482.0 75 2093.0 76 1339.5 77 789.0 78 603.0 79 507.0 80 343.5 81 243.0 82 194.0 83 178.0 84 140.0 85 104.5 86 105.0 87 103.0 88 101.0 89 83.5 90 70.5 91 73.0 92 58.0 93 45.5 94 56.5 95 57.0 96 49.0 97 54.0 98 56.0 99 120.0 100 187.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010009813488417235 2 0.012747303917570016 3 0.012968515265380341 4 0.014185177678337131 5 0.011060567390516283 6 0.00865489398307899 7 0.014268131933766005 8 0.01601017129777232 9 0.01562305143910425 10-11 0.018761487436163245 12-13 0.014973243104911417 14-15 0.007977434230409869 16-17 0.006180092029450973 18-19 0.01790429346339823 20-21 0.023489879995608955 22-23 0.021222463680553118 24-25 0.019591029990451966 26-27 0.023586659960275973 28-29 0.008115691322791323 30-31 0.007991259939648014 32-33 0.009830079268321346 34-35 0.011171173064421445 36-37 0.011019090262801845 38-39 0.013065295230047359 40-41 0.010493713311752324 42-43 0.014447866153861894 44-45 0.01330033228709583 46-47 0.023807871308086297 48-49 0.02314423726465532 50-51 0.03662430377184704 52-53 0.04053697948624217 54-55 0.028135318299625792 56-57 0.020406746835502542 58-59 0.0159133913331053 60-61 0.01972928708283342 62-63 0.02567434205523592 64-65 0.025480782125901887 66-67 0.02830122681048354 68-69 0.042652312999678414 70-71 0.05516457986019996 72-73 0.08679780259707653 74-75 0.08708814249107759 76-77 0.09214835207223879 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3616451.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.55102446687615 #Duplication Level Percentage of deduplicated Percentage of total 1 73.38374656992947 36.36239821757614 2 13.286590429422136 13.167283348993172 3 5.148860418604293 7.653939257363746 4 2.563357634763322 5.080679875100445 5 1.4567196749339109 3.609097612701504 6 0.9031854165250456 2.6852257603414955 7 0.6068151418845573 2.1047818359674833 8 0.43670422519839186 1.7311313398074968 9 0.31561901613154864 1.4075321031491168 >10 1.7061906872218615 15.364841871025053 >50 0.12597937070828819 4.294275930465471 >100 0.06398884420427804 5.540884742862897 >500 0.001962249165283115 0.6609516592958866 >1k 2.2425704605480242E-4 0.15909844208739043 >5k 5.6064261513700605E-5 0.17787800326271364 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6403 0.1770520325036894 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.7651418476290706E-5 2.7651418476290706E-5 2 0.0 0.0 0.0 1.1060567390516282E-4 2.7651418476290706E-5 3 0.0 0.0 0.0 2.4886276628661633E-4 2.7651418476290706E-5 4 0.0 0.0 0.0 0.0011060567390516283 2.7651418476290706E-5 5 0.0 0.0 0.0 0.002433324825913582 2.7651418476290706E-5 6 0.0 0.0 0.0 0.004341272700777641 2.7651418476290706E-5 7 0.0 0.0 0.0 0.009484436537367712 5.530283695258141E-5 8 0.0 0.0 0.0 0.014323434770718586 5.530283695258141E-5 9 0.0 0.0 0.0 0.019798415629024145 5.530283695258141E-5 10 0.0 0.0 0.0 0.025632864927521485 5.530283695258141E-5 11 0.0 0.0 0.0 0.03401124472583757 5.530283695258141E-5 12 0.0 0.0 0.0 0.044961206442448685 5.530283695258141E-5 13 0.0 0.0 0.0 0.057763813196971284 5.530283695258141E-5 14 0.0 0.0 0.0 0.07446526995665087 8.295425542887212E-5 15 0.0 0.0 0.0 0.09271520615100273 8.295425542887212E-5 16 0.0 0.0 0.0 0.11162877638878559 8.295425542887212E-5 17 0.0 0.0 0.0 0.13128893492542829 8.295425542887212E-5 18 0.0 0.0 0.0 0.15061727644035547 8.295425542887212E-5 19 2.7651418476290706E-5 0.0 0.0 0.1695308466781383 8.295425542887212E-5 20 2.7651418476290706E-5 0.0 0.0 0.19179023855155233 8.295425542887212E-5 21 2.7651418476290706E-5 0.0 0.0 0.2151003843270654 8.295425542887212E-5 22 2.7651418476290706E-5 0.0 0.0 0.24026317514048995 8.295425542887212E-5 23 2.7651418476290706E-5 0.0 0.0 0.2695460273068818 1.1060567390516282E-4 24 2.7651418476290706E-5 0.0 0.0 0.29775047415269834 1.3825709238145354E-4 25 2.7651418476290706E-5 0.0 0.0 0.32797347454728404 1.3825709238145354E-4 26 2.7651418476290706E-5 0.0 0.0 0.359357834517874 1.3825709238145354E-4 27 2.7651418476290706E-5 0.0 0.0 0.39165469129818153 1.3825709238145354E-4 28 2.7651418476290706E-5 0.0 0.0 0.4233432168720107 1.3825709238145354E-4 29 2.7651418476290706E-5 0.0 0.0 0.45660787329898844 1.3825709238145354E-4 30 2.7651418476290706E-5 0.0 0.0 0.49374372831264685 1.3825709238145354E-4 31 2.7651418476290706E-5 0.0 0.0 0.5333129081522189 1.3825709238145354E-4 32 2.7651418476290706E-5 0.0 0.0 0.5766426809045664 1.3825709238145354E-4 33 2.7651418476290706E-5 0.0 0.0 0.623124715363211 1.3825709238145354E-4 34 2.7651418476290706E-5 0.0 0.0 0.671625303370625 1.3825709238145354E-4 35 2.7651418476290706E-5 0.0 0.0 0.723084593155002 1.3825709238145354E-4 36 2.7651418476290706E-5 0.0 0.0 0.776258270884909 1.3825709238145354E-4 37 2.7651418476290706E-5 0.0 0.0 0.8298467198919603 1.3825709238145354E-4 38 2.7651418476290706E-5 0.0 0.0 0.888882498338841 1.3825709238145354E-4 39 2.7651418476290706E-5 0.0 0.0 0.9433281413186574 1.3825709238145354E-4 40 2.7651418476290706E-5 0.0 0.0 1.0063180726076477 1.3825709238145354E-4 41 2.7651418476290706E-5 0.0 0.0 1.0725708712768403 1.3825709238145354E-4 42 2.7651418476290706E-5 0.0 0.0 1.1436350167609073 1.3825709238145354E-4 43 2.7651418476290706E-5 0.0 0.0 1.221694971119476 1.3825709238145354E-4 44 2.7651418476290706E-5 0.0 0.0 1.3053405120102552 1.3825709238145354E-4 45 2.7651418476290706E-5 0.0 0.0 1.3898985497107523 1.3825709238145354E-4 46 2.7651418476290706E-5 0.0 0.0 1.4832220870682336 1.6590851085774425E-4 47 2.7651418476290706E-5 0.0 0.0 1.5769327442843826 1.6590851085774425E-4 48 2.7651418476290706E-5 0.0 0.0 1.6737127089514001 2.4886276628661633E-4 49 2.7651418476290706E-5 0.0 0.0 1.7714328218466115 2.4886276628661633E-4 50 2.7651418476290706E-5 0.0 0.0 1.8743237499968892 2.7651418476290707E-4 51 2.7651418476290706E-5 0.0 0.0 1.9834638987228086 2.7651418476290707E-4 52 2.7651418476290706E-5 2.7651418476290706E-5 0.0 2.1049089286706772 2.7651418476290707E-4 53 2.7651418476290706E-5 2.7651418476290706E-5 0.0 2.2310546997595155 3.0416560323919776E-4 54 2.7651418476290706E-5 2.7651418476290706E-5 0.0 2.368067478309536 3.0416560323919776E-4 55 2.7651418476290706E-5 2.7651418476290706E-5 0.0 2.5096427409081445 3.0416560323919776E-4 56 2.7651418476290706E-5 2.7651418476290706E-5 0.0 2.6618637996201246 3.0416560323919776E-4 57 2.7651418476290706E-5 2.7651418476290706E-5 0.0 2.8177348455709756 3.318170217154885E-4 58 2.7651418476290706E-5 2.7651418476290706E-5 0.0 2.9781407241519378 3.318170217154885E-4 59 2.7651418476290706E-5 2.7651418476290706E-5 0.0 3.1425837098304386 3.318170217154885E-4 60 2.7651418476290706E-5 2.7651418476290706E-5 0.0 3.312086905090101 3.318170217154885E-4 61 2.7651418476290706E-5 2.7651418476290706E-5 0.0 3.4866779613494003 3.318170217154885E-4 62 2.7651418476290706E-5 2.7651418476290706E-5 0.0 3.6786341084118104 3.594684401917792E-4 63 2.7651418476290706E-5 2.7651418476290706E-5 0.0 3.875014482430427 3.594684401917792E-4 64 2.7651418476290706E-5 2.7651418476290706E-5 0.0 4.080492173127743 3.594684401917792E-4 65 2.7651418476290706E-5 2.7651418476290706E-5 0.0 4.30214594363369 3.594684401917792E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 390 0.0 25.479546 4 CGATCGT 520 0.0 19.794611 1 CGCGGAA 500 0.0 18.460596 59 TCGCGGA 505 0.0 18.277061 58 CTCGCGC 520 0.0 17.74763 36 CCTCGCG 560 0.0 17.747137 35 CGCTCCG 585 0.0 17.596668 25 TCGCGCT 550 0.0 16.780504 37 GGTAACG 275 2.561137E-9 16.77888 1 TAGGCCG 635 0.0 16.76663 3 TTAGTCG 280 3.250534E-9 16.480625 15 TTGCGTA 540 0.0 16.43299 36 ATTCGCG 630 0.0 16.341574 56 ATCGTTC 610 0.0 16.290201 3 CGCGACT 160 4.6359652E-4 15.532182 54 CGCGCTT 620 0.0 15.457611 38 GCGTAAG 600 0.0 15.381277 38 ACCGCTA 255 3.3302967E-7 15.312526 37 GCGGTTA 650 0.0 15.287727 3 CGTAAGT 605 0.0 15.254793 39 >>END_MODULE