FastQCFastQC Report
Thu 26 May 2016
SRR936135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3616451
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69130.19115425592659765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51430.1422112452235631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13600.043.7686100-101
GGTATCA85300.035.1599241
ACGTATC5800.034.380993100-101
CGTACAT20650.032.28491100-101
GTATCAA153300.031.8689251
TACGCAG12500.031.191257100-101
GCGTATC4800.028.522537100-101
CGGTATC14100.028.073988100-101
ATACACG10350.027.60249996-97
TCGAGTA6050.027.548899100-101
TCGTATC5350.027.25925100-101
AGTATCA32800.026.586756100-101
CGAGTAC17050.026.184149100-101
CCGTACA9950.026.023624100-101
CCTGTCG8550.025.06016396-97
TATCAAC197350.024.5105922
ATCAACG200800.023.999583
TCAACGC202100.023.8151054
CGTCCTA5250.023.810118100-101
CAACGCA206100.023.3233915
Produced by FastQC (version 0.11.5)