FastQCFastQC Report
Thu 26 May 2016
SRR936134_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936134_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3619770
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66210.18291217397790469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA4300.023.1189859
CGGTTAC3200.022.1833464
CAATTCG4950.020.08121154
CGATCGT5100.019.486011
TAGCGTC4800.019.2250351
TCGCGCT4700.018.88022837
CGTCGTG4550.018.721774
ACGATCG4200.018.5974159
ATTCGCG6000.018.3410256
CTCGCGC5050.018.2740636
CCTCGCG5300.018.0800235
TCGCGGA5450.017.58872458
AATTCGC5550.017.2701455
ATACGTA3004.5474735E-1016.5631071
CGCGCTT5800.016.52413738
CGCTCCG5900.016.24384325
CGCTATC2854.107278E-916.19218839
GATCGCG5050.016.1704361
AACTCGA6250.015.9032213
GACGTAT4500.015.77700439
Produced by FastQC (version 0.11.5)