##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936134_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3619770 Sequences flagged as poor quality 0 Sequence length 77 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.45839045022198 39.0 36.0 41.0 30.0 41.0 2 36.319717551115126 39.0 36.0 41.0 29.0 41.0 3 36.18449404243916 39.0 36.0 41.0 29.0 41.0 4 36.08209112733682 39.0 35.0 41.0 28.0 41.0 5 36.011652674064926 39.0 35.0 41.0 28.0 41.0 6 35.92458719752913 39.0 35.0 40.0 28.0 41.0 7 35.78508689778632 39.0 35.0 40.0 27.0 41.0 8 35.66204068214279 39.0 35.0 40.0 27.0 41.0 9 35.837737756818804 39.0 35.0 40.0 28.0 41.0 10-11 36.07648400312727 39.0 35.0 41.0 29.0 41.0 12-13 36.00866878282322 39.0 35.0 41.0 28.5 41.0 14-15 35.862832445155355 39.0 35.0 41.0 27.5 41.0 16-17 35.7219128563417 39.0 35.0 41.0 27.0 41.0 18-19 35.57763007041884 39.0 35.0 41.0 27.0 41.0 20-21 35.39601922221578 39.0 35.0 41.0 26.0 41.0 22-23 35.14611397961749 39.0 34.5 41.0 25.5 41.0 24-25 34.93224735273236 39.0 34.0 41.0 24.0 41.0 26-27 33.980725570961695 38.0 33.0 39.5 23.5 40.5 28-29 33.99232161159411 38.0 33.0 39.5 23.0 40.5 30-31 34.40184086281725 38.0 34.0 40.0 23.0 41.0 32-33 34.27393411736105 38.0 34.0 40.0 23.0 41.0 34-35 34.01211416747473 38.0 33.0 40.0 22.5 41.0 36-37 33.69960398036339 37.5 33.0 40.0 22.0 41.0 38-39 33.38451890589734 37.0 33.0 40.0 20.5 41.0 40-41 33.01809037590786 36.5 32.0 39.5 19.5 41.0 42-43 32.64572555714866 36.0 32.0 39.0 18.0 41.0 44-45 32.27996115775312 35.5 32.0 39.0 17.0 40.5 46-47 31.8648787077632 35.0 31.0 38.5 13.0 40.0 48-49 31.443293220287476 35.0 31.0 37.5 9.5 40.0 50-51 30.993589233570088 35.0 31.0 37.0 7.0 39.0 52-53 30.504623221917413 35.0 30.0 36.5 4.0 39.0 54-55 30.048491202479717 34.0 30.0 36.0 2.0 38.5 56-57 29.624300438978167 34.0 29.5 35.5 2.0 37.0 58-59 29.20695983446462 34.0 29.0 35.0 2.0 37.0 60-61 28.861215077201038 34.0 29.0 35.0 2.0 36.0 62-63 28.493496824383868 34.0 29.0 35.0 2.0 36.0 64-65 28.126272525602456 34.0 28.5 35.0 2.0 35.5 66-67 27.783417178439514 34.0 27.0 35.0 2.0 35.0 68-69 27.425373849719733 33.5 27.0 35.0 2.0 35.0 70-71 26.982690889200143 33.0 26.0 35.0 2.0 35.0 72-73 26.506148180685514 33.0 25.0 35.0 2.0 35.0 74-75 25.939512455211243 33.0 24.5 35.0 2.0 35.0 76-77 24.171619881926198 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.2177592205278316 1101 2 1.0955755805206948 1101 3 1.1035969754313655 1101 4 1.036513580577008 1101 5 1.1657688232443775 1101 6 1.3127328169595458 1101 7 1.2823485600927427 1101 8 1.1531549693196368 1101 9 1.1864117120200461 1101 10-11 1.1461203804125972 1101 12-13 1.3093346153731105 1101 14-15 1.3618805476612224 1101 16-17 1.360960872691379 1101 18-19 1.3857478486022998 1101 20-21 1.3493780626819998 1101 22-23 1.3697198404946604 1101 24-25 1.4343886576389053 1101 26-27 1.4009078554540224 1101 28-29 1.3880478431166878 1101 30-31 1.3943617342754422 1101 32-33 1.4671110892828878 1101 34-35 1.4365453362974279 1101 36-37 1.4203593391686624 1101 38-39 1.368759400345347 1101 40-41 1.2934288155689586 1101 42-43 1.2960675055475548 1101 44-45 1.3233492042225095 1101 46-47 1.3478663988628874 1101 48-49 1.3203321726778938 1101 50-51 1.187139277849468 1101 52-53 1.2488103355192273 1101 54-55 1.3528266024919091 1101 56-57 1.3845694596763742 1101 58-59 1.3081266364105915 1101 60-61 1.286993687694018 1101 62-63 1.3921081092941492 1101 64-65 1.3260867696992413 1101 66-67 1.4220846376116931 1101 68-69 1.377189129368535 1101 70-71 1.242398935423921 1101 72-73 1.1626803196085334 1101 74-75 1.210968363386442 1101 76-77 1.3967528294951812 1102 1 0.6632821633561647 1102 2 0.7840065297300853 1102 3 0.8350045789946563 1102 4 0.6420559262391166 1102 5 0.5789279816131128 1102 6 0.6634251655560846 1102 7 0.6182633654686285 1102 8 0.7368666371630397 1102 9 0.6587343966181791 1102 10-11 0.7945759344070709 1102 12-13 0.8102833268008638 1102 14-15 0.9380549437457901 1102 16-17 0.9967763519200332 1102 18-19 0.9908919559919198 1102 20-21 0.9603023382769322 1102 22-23 0.8539561495812649 1102 24-25 0.8936521769323633 1102 26-27 0.685156569193758 1102 28-29 0.8470932361441115 1102 30-31 0.9671576185982715 1102 32-33 0.9635681602908548 1102 34-35 0.8450359879018521 1102 36-37 0.850449781983663 1102 38-39 0.989059237261948 1102 40-41 0.9177634993347823 1102 42-43 0.849544721000818 1102 44-45 0.7677656431000273 1102 46-47 0.8541260358111451 1102 48-49 0.7535014148291737 1102 50-51 0.6853365571844776 1102 52-53 0.8573117911763717 1102 54-55 0.7723225823172335 1102 56-57 0.9119844989177608 1102 58-59 1.0282708593991252 1102 60-61 0.905014136253385 1102 62-63 0.9205099186839796 1102 64-65 0.9639797837898634 1102 66-67 0.9730200107027507 1102 68-69 1.101510874947767 1102 70-71 1.0321041848597083 1102 72-73 0.8839364708048052 1102 74-75 0.8749482296118174 1102 76-77 1.0690273182367385 1103 1 0.4355506922786816 1103 2 0.5166735133476408 1103 3 0.4330988082675944 1103 4 0.46584666637966876 1103 5 0.3871075730626643 1103 6 0.3290762342489799 1103 7 0.33946929960231387 1103 8 0.47422803231911104 1103 9 0.4482007107993269 1103 10-11 0.4061610916196088 1103 12-13 0.5390243730324045 1103 14-15 0.4544390837040808 1103 16-17 0.42153702953241634 1103 18-19 0.36967856861883064 1103 20-21 0.32320169357172546 1103 22-23 0.24231057595850558 1103 24-25 0.3909102882589153 1103 26-27 0.3966795031501462 1103 28-29 0.2754890618783392 1103 30-31 0.4433439541001931 1103 32-33 0.3550207627700743 1103 34-35 0.5405626891319812 1103 36-37 0.5610930078360141 1103 38-39 0.43142028798801135 1103 40-41 0.5600560028440142 1103 42-43 0.5037691832100748 1103 44-45 0.6134401104474989 1103 46-47 0.5013444529915425 1103 48-49 0.4431857927264744 1103 50-51 0.5065903727348697 1103 52-53 0.5467166725964638 1103 54-55 0.6240234454249389 1103 56-57 0.5697244057070989 1103 58-59 0.49204246955856945 1103 60-61 0.48164247143685657 1103 62-63 0.30253981972528976 1103 64-65 0.19794205007151788 1103 66-67 0.31577190478544637 1103 68-69 0.18841846945992202 1103 70-71 0.21852438268674845 1103 72-73 0.2443869852477114 1103 74-75 0.34193037910347357 1103 76-77 0.3269664297880155 1104 1 0.4947027435537237 1104 2 0.40370076899267104 1104 3 0.5036680562912323 1104 4 0.5327165702810532 1104 5 0.5037643429962344 1104 6 0.5803315762754409 1104 7 0.581377552467707 1104 8 0.49294948101566405 1104 9 0.5031174372602152 1104 10-11 0.6057604527858373 1104 12-13 0.6240026892972068 1104 14-15 0.7297642766062111 1104 16-17 0.775479892206306 1104 18-19 0.6030431141862422 1104 20-21 0.47999969711099055 1104 22-23 0.5575236573214326 1104 24-25 0.6590509158562767 1104 26-27 0.4788437103629093 1104 28-29 0.5647468910639546 1104 30-31 0.7694750328581321 1104 32-33 0.6165657052308475 1104 34-35 0.758830262847205 1104 36-37 0.6585097290364104 1104 38-39 0.6984709843715322 1104 40-41 0.7139106576402199 1104 42-43 0.8483808644412818 1104 44-45 0.7534933045111387 1104 46-47 0.6730120069273333 1104 48-49 0.6679305012007006 1104 50-51 0.5814273922681181 1104 52-53 0.5658238624222527 1104 54-55 0.6681402198614883 1104 56-57 0.5798305202889615 1104 58-59 0.4049980015938921 1104 60-61 0.5527128967579031 1104 62-63 0.390566947014392 1104 64-65 0.29210947249508834 1104 66-67 0.38726363214061976 1104 68-69 0.21127735579338136 1104 70-71 0.33137386326607654 1104 72-73 0.2121327815910412 1104 74-75 0.2553139886826088 1104 76-77 0.3277574389812479 1105 1 0.07050908560454872 1105 2 0.2580382089119837 1105 3 0.22950867135282493 1105 4 0.21483676253926376 1105 5 0.11689018566973886 1105 6 0.23420874921520607 1105 7 0.4062175602897753 1105 8 0.2593104694781161 1105 9 0.3032360033515502 1105 10-11 0.39703678794088404 1105 12-13 0.5327131801319993 1105 14-15 0.4899765016759332 1105 16-17 0.44166941269291726 1105 18-19 0.33470808264066676 1105 20-21 0.24103509197106376 1105 22-23 0.2810075654738853 1105 24-25 0.3870166634298968 1105 26-27 0.08284119906722509 1105 28-29 0.23657118270360655 1105 30-31 0.44856170343583557 1105 32-33 0.48380688224631996 1105 34-35 0.461073961450559 1105 36-37 0.3898369076368837 1105 38-39 0.3405358046183409 1105 40-41 0.5691762477432363 1105 42-43 0.5128635272380819 1105 44-45 0.3091996064223963 1105 46-47 0.4961503031573038 1105 48-49 0.35251073401015987 1105 50-51 0.3020249571346767 1105 52-53 0.3622399141302459 1105 54-55 0.18767171912559633 1105 56-57 0.2294271691501315 1105 58-59 0.3787456987151643 1105 60-61 0.44781710523661644 1105 62-63 0.2525805121279632 1105 64-65 0.3769509212245161 1105 66-67 0.38082895155890384 1105 68-69 0.22719341264746973 1105 70-71 0.13255231964260616 1105 72-73 -0.34751131429105087 1105 74-75 -0.5482259090129666 1105 76-77 -0.5107767465458686 1106 1 0.32242445718480184 1106 2 0.44650021436399356 1106 3 0.4099840026825703 1106 4 0.44013189036674305 1106 5 0.5115132979622672 1106 6 0.3658330598458974 1106 7 0.03137981917548416 1106 8 0.3383755301848481 1106 9 0.42131060568885914 1106 10-11 0.16316207052089027 1106 12-13 0.4857578971093872 1106 14-15 0.28848132000242543 1106 16-17 0.31391239230330825 1106 18-19 0.3957214438472434 1106 20-21 0.6061322239592215 1106 22-23 0.6946746711115921 1106 24-25 0.5328450375274372 1106 26-27 0.4446203028272322 1106 28-29 0.6143529535883303 1106 30-31 0.7438375059792648 1106 32-33 0.6722338837167214 1106 34-35 0.660113301134146 1106 36-37 0.787312560174847 1106 38-39 0.8985957962449973 1106 40-41 0.8607082079621478 1106 42-43 0.9961187513386847 1106 44-45 0.8032703298962289 1106 46-47 0.8047417908549441 1106 48-49 0.7207431588236801 1106 50-51 0.6838038822200687 1106 52-53 0.7441201558445165 1106 54-55 0.7754153307974327 1106 56-57 0.6843200719913405 1106 58-59 0.5976450874449384 1106 60-61 0.37860960431380164 1106 62-63 0.48280929279147244 1106 64-65 0.5507668871821316 1106 66-67 0.45573536057677444 1106 68-69 0.4730439992484179 1106 70-71 0.35501419596160133 1106 72-73 0.3094661122016049 1106 74-75 0.3381432075669437 1106 76-77 0.37180377783976226 1107 1 0.1532495258269435 1107 2 0.09485922936527658 1107 3 0.12564248769002262 1107 4 0.13082618655520406 1107 5 0.2823890711207184 1107 6 -0.00442725414312406 1107 7 -0.0516340902017447 1107 8 0.032179273652147344 1107 9 -0.03348187972942185 1107 10-11 0.15188825642527348 1107 12-13 0.18350706390968696 1107 14-15 -0.030707531993421355 1107 16-17 0.002700512426955015 1107 18-19 0.1953882804608824 1107 20-21 0.17312373139928638 1107 22-23 0.054154664417261245 1107 24-25 0.22475887479692602 1107 26-27 0.015161359390063467 1107 28-29 0.06289548026373382 1107 30-31 0.3652811130281961 1107 32-33 0.4539312929248567 1107 34-35 0.22861837216823488 1107 36-37 0.26258535225876045 1107 38-39 0.34871692715218927 1107 40-41 0.33836459380728456 1107 42-43 0.34887393100034103 1107 44-45 0.33180841439313724 1107 46-47 0.31482188597348326 1107 48-49 0.3606722858851299 1107 50-51 0.43543461603766787 1107 52-53 0.36288731152617615 1107 54-55 0.2861602771398779 1107 56-57 0.20187226855929552 1107 58-59 0.21764747162115583 1107 60-61 0.2565440534467811 1107 62-63 0.14245113050207436 1107 64-65 0.2535835545153837 1107 66-67 0.29796051922739153 1107 68-69 0.09777244973190946 1107 70-71 0.15929953914124795 1107 72-73 0.2595732642254518 1107 74-75 0.1285431317703285 1107 76-77 0.2396335523176596 1108 1 0.22488039260778692 1108 2 0.33512103213629985 1108 3 0.38473534454889347 1108 4 0.3393557971127237 1108 5 0.2497120240333217 1108 6 0.16404354319276138 1108 7 0.20042510882520048 1108 8 0.14876257004928561 1108 9 0.25193158723195097 1108 10-11 0.37645610293955656 1108 12-13 0.38763028452995485 1108 14-15 0.4023080887039967 1108 16-17 0.41015564783430847 1108 18-19 0.27333704050912644 1108 20-21 0.3709887702227874 1108 22-23 0.2528059288131814 1108 24-25 0.37682418486217983 1108 26-27 0.24516589481125806 1108 28-29 0.40414734486667925 1108 30-31 0.5413238917688474 1108 32-33 0.5383015393837596 1108 34-35 0.475808951155976 1108 36-37 0.5281171819330837 1108 38-39 0.6542229726624953 1108 40-41 0.5316102030630745 1108 42-43 0.5480232818313695 1108 44-45 0.6003062696395745 1108 46-47 0.5701698543845701 1108 48-49 0.5124273687228325 1108 50-51 0.409000399524313 1108 52-53 0.5788991890285722 1108 54-55 0.630166379342942 1108 56-57 0.6173239184660098 1108 58-59 0.6092003232672312 1108 60-61 0.652878387616024 1108 62-63 0.5756882579312119 1108 64-65 0.6180719059928528 1108 66-67 0.7160863005935845 1108 68-69 0.7651863319493799 1108 70-71 0.751329567163669 1108 72-73 0.764945971297621 1108 74-75 0.7200053486194236 1108 76-77 0.6090592177950107 1109 1 -0.10439687924735352 1109 2 -0.22174618901357235 1109 3 -0.12658531310882637 1109 4 -0.07942745687568475 1109 5 0.040611639671396915 1109 6 -0.19797219433070268 1109 7 -0.0162408581594633 1109 8 0.1516648706923931 1109 9 2.444420557168314E-4 1109 10-11 0.044853989821078244 1109 12-13 0.11836640074496785 1109 14-15 0.23917551828026973 1109 16-17 0.17714391446281752 1109 18-19 0.07435682948700162 1109 20-21 0.12572758829433184 1109 22-23 0.21328662069421256 1109 24-25 0.08244953944574007 1109 26-27 0.03343342502348179 1109 28-29 0.24151562603477572 1109 30-31 0.22032251526769642 1109 32-33 0.22601796654731032 1109 34-35 0.1630835808739164 1109 36-37 0.3054486085836956 1109 38-39 0.24925025878227558 1109 40-41 0.279448111774677 1109 42-43 0.3281241557736081 1109 44-45 0.24309952779947253 1109 46-47 0.13990496660518303 1109 48-49 0.21764093334935097 1109 50-51 0.1570397131776602 1109 52-53 0.24728088960468497 1109 54-55 0.3807272828669461 1109 56-57 0.28778833091494604 1109 58-59 0.398546497046361 1109 60-61 0.2903894778220817 1109 62-63 0.2552388787905855 1109 64-65 0.40301943279930086 1109 66-67 0.513393697602119 1109 68-69 0.35034654976531243 1109 70-71 0.4668408371190189 1109 72-73 0.32095295313850514 1109 74-75 0.3370395799206882 1109 76-77 0.46033944368783253 1110 1 -0.341365506737489 1110 2 -0.33356767625635797 1110 3 -0.6147872605434515 1110 4 -0.4680746272605205 1110 5 -0.48932436469311824 1110 6 -0.5204184844136464 1110 7 -0.4812446301942188 1110 8 -0.7199956428747569 1110 9 -0.6967229080936335 1110 10-11 -0.6736306615787484 1110 12-13 -0.5896747684227321 1110 14-15 -0.6262425130444669 1110 16-17 -0.642677756406691 1110 18-19 -0.6091876661907634 1110 20-21 -0.7224210542293505 1110 22-23 -0.6591054542467489 1110 24-25 -0.7075509655995731 1110 26-27 -0.7693908487332095 1110 28-29 -0.6550672215486486 1110 30-31 -0.49790720678567624 1110 32-33 -0.43725650302399544 1110 34-35 -0.5431979759271925 1110 36-37 -0.5746580156064809 1110 38-39 -0.4629760693378415 1110 40-41 -0.5147144192796773 1110 42-43 -0.480030285791031 1110 44-45 -0.4005042528706255 1110 46-47 -0.4964598192214815 1110 48-49 -0.4208276212576507 1110 50-51 -0.47428808975379866 1110 52-53 -0.4911341934192315 1110 54-55 -0.4264532761499673 1110 56-57 -0.3178359701127498 1110 58-59 -0.2711938098013533 1110 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-1.005391381307156 2314 40-41 -0.812595313887222 2314 42-43 -0.7707209204213825 2314 44-45 -0.8193527684189483 2314 46-47 -0.5901645209617818 2314 48-49 -0.4591608863380685 2314 50-51 -0.44521772887051725 2314 52-53 -0.3936046229981507 2314 54-55 -0.5069883937497401 2314 56-57 -0.5066694306670563 2314 58-59 -0.4659840280151428 2314 60-61 -0.436089483058975 2314 62-63 -0.5304022296893827 2314 64-65 -0.35697665211720775 2314 66-67 -0.2931206359033389 2314 68-69 -0.2986822590253375 2314 70-71 -0.24702327797720613 2314 72-73 -0.288928733090021 2314 74-75 -0.212660190507183 2314 76-77 -0.21886769250735583 2315 1 -1.1496469573568149 2315 2 -1.1051758088690562 2315 3 -0.905279796138565 2315 4 -0.9015929723252754 2315 5 -0.9794168111996626 2315 6 -1.0579411885167715 2315 7 -1.2955768554413396 2315 8 -1.3160319365639097 2315 9 -1.042314054727818 2315 10-11 -1.1598806501375876 2315 12-13 -1.1602422746459453 2315 14-15 -1.1758213753197637 2315 16-17 -0.959639084262129 2315 18-19 -1.0097188352639606 2315 20-21 -0.8951445559702407 2315 22-23 -1.0509580847783369 2315 24-25 -1.1482991326933103 2315 26-27 -1.0647279640756935 2315 28-29 -1.0416943981802973 2315 30-31 -1.1154434945907994 2315 32-33 -1.0970174258838696 2315 34-35 -1.2083312043608387 2315 36-37 -1.2570622399534983 2315 38-39 -1.2478868715003983 2315 40-41 -1.2843248772358606 2315 42-43 -1.4113147544504194 2315 44-45 -1.213282471725762 2315 46-47 -1.1087611577354153 2315 48-49 -1.0649861219397287 2315 50-51 -1.1398336983594994 2315 52-53 -1.1467541294570331 2315 54-55 -1.2842801528144285 2315 56-57 -1.3523993187866914 2315 58-59 -1.1903460326288595 2315 60-61 -1.2603605920825416 2315 62-63 -1.1170698095646365 2315 64-65 -1.183751670535262 2315 66-67 -1.1794962520253627 2315 68-69 -1.259706633972673 2315 70-71 -1.2308591781988731 2315 72-73 -1.1342268895389935 2315 74-75 -1.1828796582565388 2315 76-77 -1.150634447842549 2316 1 -2.2532329287852306 2316 2 -1.934860832665457 2316 3 -2.231780495097304 2316 4 -3.041623256396221 2316 5 -2.5537994514796623 2316 6 -2.3692984232739747 2316 7 -2.1415054021289492 2316 8 -1.9101546327705492 2316 9 -2.1157247249181808 2316 10-11 -2.0481102085139398 2316 12-13 -2.638781626048022 2316 14-15 -3.790372774504469 2316 16-17 -3.009467164761901 2316 18-19 -2.428091331075926 2316 20-21 -2.2017686001509915 2316 22-23 -2.5215182990925555 2316 24-25 -2.4118678898089954 2316 26-27 -2.4199072471174503 2316 28-29 -2.993248332798707 2316 30-31 -3.089878761767963 2316 32-33 -3.243581408810698 2316 34-35 -3.0807900868020894 2316 36-37 -3.1201973345402862 2316 38-39 -3.1490837234279887 2316 40-41 -2.916162336156816 2316 42-43 -2.976854187600594 2316 44-45 -2.8462772005879096 2316 46-47 -2.7940091402467893 2316 48-49 -2.6815276775590036 2316 50-51 -2.749315161914005 2316 52-53 -2.9122978239447264 2316 54-55 -2.9474620165122545 2316 56-57 -2.743395174729134 2316 58-59 -2.9436566446265005 2316 60-61 -2.6981032895414927 2316 62-63 -2.6687786245450624 2316 64-65 -2.6511845227888493 2316 66-67 -2.5313512880318036 2316 68-69 -2.663628660938116 2316 70-71 -2.4980249141629223 2316 72-73 -2.408402618898588 2316 74-75 -2.556915701469233 2316 76-77 -3.032370187518808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93539.0 3 7878.0 4 9763.0 5 11749.0 6 13690.0 7 14323.0 8 16257.0 9 16280.0 10 18588.0 11 20635.0 12 17727.0 13 16253.0 14 16772.0 15 17496.0 16 17847.0 17 19007.0 18 20099.0 19 21245.0 20 22830.0 21 24532.0 22 27336.0 23 31182.0 24 35832.0 25 42626.0 26 50008.0 27 59419.0 28 69702.0 29 81758.0 30 97385.0 31 117009.0 32 143535.0 33 179953.0 34 236832.0 35 316613.0 36 449936.0 37 607439.0 38 526876.0 39 128994.0 40 825.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.95441071082249 26.196422542853405 27.92288017734233 22.92628656898178 2 23.027749182620553 26.493233584583926 27.712409524646265 22.766607708149262 3 23.07224949376423 26.712753991657646 27.22375579556723 22.991240719010896 4 22.834365873856438 26.549936765470218 27.57688652904188 23.03881083163147 5 23.098221541051107 26.690340033879885 27.546537866749997 22.664900558319015 6 23.243265496486586 26.511906261811767 27.4320084260039 22.812819815697743 7 23.08260656806256 26.271559459636446 27.57853499705315 23.067298975247844 8 23.13784209906788 26.392642939287448 27.521197528414888 22.94831743322979 9 23.027703349982993 26.226184295346926 27.55231766633242 23.193794688337658 10-11 23.30644944020481 26.431824634580536 27.347834286531413 22.91389163868324 12-13 23.347545809226215 26.378656445068373 27.288658328183434 22.985139417521978 14-15 23.081699581155306 26.491001469824393 27.44961983489341 22.977679114126893 16-17 23.272155682667105 26.325089071402996 27.400120677384702 23.002634568545197 18-19 23.14356271509375 26.25244795200909 27.383244293453636 23.220745039443525 20-21 23.181816109652384 26.409711660892 27.325691830541597 23.082780398914018 22-23 23.319496732140816 26.590008387060855 26.96529171457579 23.125203166222537 24-25 23.312556938898503 26.539847488719985 27.02278329632046 23.124812276061057 26-27 23.244466793855693 26.519464479862737 27.04559049646546 23.19047822981611 28-29 23.346758830893158 26.565300384226358 26.906205613456734 23.18173517142375 30-31 23.229944272435556 26.47718935588462 26.932157585467646 23.360708786212175 32-33 23.133579808232525 26.81695805879121 26.90850548379713 23.140956649179135 34-35 23.381408959080414 26.52063192265388 26.896567825257844 23.201391293007866 36-37 23.299944729148653 26.53021586023396 27.155946548210313 23.013892862407076 38-39 23.146041430394863 26.466278242611637 27.19834149108583 23.18933883590767 40-41 23.149360135771264 26.485979074813038 27.04321043510215 23.32145035431355 42-43 22.992514982468645 26.699809075411068 26.917369164159226 23.390306777961058 44-45 22.98866343412138 26.445530617238205 27.15995655861444 23.40584939002597 46-47 23.02086821684864 26.368842955933765 27.158274176417475 23.452014650800123 48-49 23.09441377464868 26.4221967506579 27.00924615942949 23.474143315263934 50-51 22.995908904384972 26.616341999566156 26.912044898427258 23.47570419762161 52-53 23.09525112292762 26.534833554685623 26.97028407706044 23.399631245326315 54-55 23.017306411634504 26.542530997073687 27.033178681861013 23.406983909430792 56-57 22.90726082309812 26.528595790676622 27.072955821224774 23.49118756500048 58-59 23.071731718234517 26.62608657209644 26.98221696627413 23.319964743394912 60-61 23.05534498029803 26.374305775168626 26.940710619306785 23.629638625226562 62-63 22.936099753842957 26.298403792609097 27.173935870950483 23.59156058259746 64-65 23.06284581862121 26.31912417645621 27.03955013478024 23.578479870142342 66-67 22.966331141832132 26.430231194235294 27.152601789996922 23.450835873935652 68-69 22.951069510330335 26.137237717429194 27.212917329824187 23.69877544241628 70-71 22.942803174740504 26.224378200284736 27.254799226424087 23.57801939855067 72-73 22.970813137149936 26.198553380332097 27.17971918234156 23.650914300176414 74-75 22.799290383754307 26.139831117084327 27.100962167854682 23.95991633130668 76-77 22.70433948388452 25.92484973818226 27.45276498332882 23.9180457946044 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6278.0 1 3838.5 2 1300.5 3 1241.5 4 1281.0 5 1324.0 6 1407.5 7 1557.5 8 1716.0 9 1765.0 10 1805.5 11 1923.5 12 2057.5 13 2114.0 14 2290.0 15 2615.5 16 2999.0 17 3233.0 18 3495.5 19 4173.0 20 5181.0 21 6756.0 22 7738.0 23 8996.0 24 11989.0 25 16438.0 26 19152.0 27 23022.0 28 31900.0 29 41509.0 30 46110.0 31 52757.0 32 66266.5 33 79715.5 34 92362.5 35 98423.0 36 106187.0 37 120396.0 38 130456.0 39 134071.0 40 139013.5 41 149004.5 42 158642.5 43 163232.0 44 167223.0 45 173279.0 46 177165.0 47 179548.5 48 180111.0 49 179107.0 50 177598.0 51 172786.5 52 168480.0 53 166013.5 54 157834.5 55 145034.0 56 129789.5 57 121633.0 58 113974.0 59 96997.0 60 79208.5 61 70738.0 62 62855.5 63 46636.0 64 32929.0 65 27559.0 66 23604.5 67 17697.5 68 13232.5 69 9206.0 70 7692.0 71 6531.0 72 4518.0 73 3079.5 74 2493.0 75 2089.5 76 1371.5 77 850.5 78 644.0 79 507.0 80 331.5 81 250.0 82 177.5 83 148.0 84 140.0 85 113.5 86 87.5 87 80.0 88 76.5 89 65.5 90 50.5 91 43.0 92 42.5 93 34.5 94 32.5 95 32.0 96 26.0 97 31.0 98 37.0 99 72.5 100 107.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0028731107225044685 2 0.007624793840492628 3 0.011299060437541612 4 0.01942112344154463 5 0.01417217116004608 6 0.01375778019045409 7 0.017984568080292394 8 0.019780262281857686 9 0.01870284576091851 10-11 0.022446177519566162 12-13 0.02117537854615072 14-15 0.008011558745445152 16-17 0.005580465057172141 18-19 0.011547695019296807 20-21 0.010180204819643237 22-23 0.013937349610610618 24-25 0.020968183061354725 26-27 0.01339864135014103 28-29 0.007983932680805685 30-31 0.007914867519207022 32-33 0.00924091862190139 34-35 0.011133304049704815 36-37 0.008881779781588333 38-39 0.01700384278559135 40-41 0.012362663926161055 42-43 0.014918074905311662 44-45 0.017694494401578 46-47 0.016078369620169237 48-49 0.013357202253181833 50-51 0.025830370437900752 52-53 0.030499175361970512 54-55 0.024310936882730116 56-57 0.015636352585937784 58-59 0.016299378137284964 60-61 0.019766449249537955 62-63 0.026023752890377012 64-65 0.020180840219129945 66-67 0.01971119712025902 68-69 0.02857916386952762 70-71 0.05595659392723847 72-73 0.06742141075261687 74-75 0.07971500951717927 76-77 0.07597167775853163 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3619770.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.65417179439351 #Duplication Level Percentage of deduplicated Percentage of total 1 73.52766428876888 36.50955274235023 2 13.304200650591483 13.21218129383103 3 5.05284603220931 7.526846548018221 4 2.5288649694505514 5.022747825516856 5 1.391157036348485 3.4538375237913512 6 0.9228330655429992 2.7493506944411337 7 0.6259315768249916 2.1756079833042627 8 0.4258123963102629 1.6914689502857734 9 0.3203843571468992 1.4317577919007614 >10 1.7083569320693044 15.37675373908419 >50 0.1257301689242301 4.295212463457264 >100 0.0638722466096966 5.529293570267688 >500 0.002066960252827721 0.6812347946858895 >1k 2.234551601020197E-4 0.16049613133493443 >5k 5.586379002550487E-5 0.1836579477304874 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6621 0.18291217397790469 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.7626064639466044E-5 2.7626064639466044E-5 2 0.0 0.0 0.0 2.2100851711572835E-4 5.525212927893209E-5 3 0.0 0.0 0.0 4.6964309887092274E-4 5.525212927893209E-5 4 0.0 0.0 0.0 0.0011326686502181077 5.525212927893209E-5 5 0.0 0.0 0.0 0.0021548330418783516 5.525212927893209E-5 6 0.0 0.0 0.0 0.004337292148396169 5.525212927893209E-5 7 0.0 0.0 0.0 0.008840340684629133 5.525212927893209E-5 8 0.0 0.0 0.0 0.013785406255093556 5.525212927893209E-5 9 0.0 0.0 0.0 0.01942112344154463 5.525212927893209E-5 10 0.0 0.0 0.0 0.025940874696458616 1.1050425855786418E-4 11 0.0 0.0 0.0 0.033924807377264304 1.1050425855786418E-4 12 0.0 0.0 0.0 0.04447796406954033 1.1050425855786418E-4 13 0.0 0.0 0.0 0.05806998787215762 1.1050425855786418E-4 14 0.0 0.0 0.0 0.07511526975470817 1.1050425855786418E-4 15 0.0 0.0 0.0 0.09395624583882402 1.6575638783679626E-4 16 0.0 0.0 0.0 0.11257621340582413 1.6575638783679626E-4 17 0.0 0.0 0.0 0.13185920652417144 1.6575638783679626E-4 18 0.0 0.0 0.0 0.1526340071330499 1.6575638783679626E-4 19 0.0 0.0 0.0 0.17202750450995505 1.6575638783679626E-4 20 0.0 0.0 0.0 0.1955649115827801 1.6575638783679626E-4 21 0.0 0.0 0.0 0.21929570110808144 1.6575638783679626E-4 22 0.0 0.0 0.0 0.24589960135588726 1.6575638783679626E-4 23 0.0 0.0 0.0 0.27402293515886367 1.6575638783679626E-4 24 0.0 0.0 0.0 0.3028092945131873 1.6575638783679626E-4 25 0.0 0.0 0.0 0.333557104456913 1.6575638783679626E-4 26 0.0 2.7626064639466044E-5 0.0 0.3655480873094147 1.6575638783679626E-4 27 0.0 2.7626064639466044E-5 0.0 0.39698654886912704 1.6575638783679626E-4 28 0.0 2.7626064639466044E-5 0.0 0.4276238545542949 1.6575638783679626E-4 29 0.0 2.7626064639466044E-5 0.0 0.46085801031557255 2.2100851711572835E-4 30 0.0 2.7626064639466044E-5 0.0 0.49597073847233386 2.2100851711572835E-4 31 0.0 2.7626064639466044E-5 0.0 0.5351168720664573 2.2100851711572835E-4 32 0.0 2.7626064639466044E-5 0.0 0.5779372722576296 2.2100851711572835E-4 33 0.0 2.7626064639466044E-5 0.0 0.622774375167483 2.2100851711572835E-4 34 0.0 2.7626064639466044E-5 0.0 0.6702912063473646 2.2100851711572835E-4 35 0.0 2.7626064639466044E-5 0.0 0.720045748763043 2.2100851711572835E-4 36 0.0 2.7626064639466044E-5 0.0 0.7717617417681234 2.2100851711572835E-4 37 0.0 2.7626064639466044E-5 0.0 0.8275940184044842 2.2100851711572835E-4 38 0.0 2.7626064639466044E-5 0.0 0.8827356434248584 2.2100851711572835E-4 39 0.0 2.7626064639466044E-5 0.0 0.9373799992817223 2.2100851711572835E-4 40 0.0 2.7626064639466044E-5 0.0 0.9974390638079215 2.2100851711572835E-4 41 0.0 2.7626064639466044E-5 0.0 1.0639902535243952 2.2100851711572835E-4 42 0.0 2.7626064639466044E-5 0.0 1.1351549960356597 2.2100851711572835E-4 43 0.0 2.7626064639466044E-5 0.0 1.2108504131477966 2.2100851711572835E-4 44 0.0 2.7626064639466044E-5 0.0 1.2922367995756636 2.2100851711572835E-4 45 0.0 2.7626064639466044E-5 0.0 1.3776289653762532 2.2100851711572835E-4 46 0.0 2.7626064639466044E-5 0.0 1.470977437793009 2.2100851711572835E-4 47 0.0 2.7626064639466044E-5 0.0 1.5621434511032468 2.2100851711572835E-4 48 0.0 2.7626064639466044E-5 0.0 1.6563759575884656 2.486345817551944E-4 49 0.0 2.7626064639466044E-5 0.0 1.7535920790547466 2.486345817551944E-4 50 0.0 2.7626064639466044E-5 0.0 1.8570240650649075 2.486345817551944E-4 51 0.0 2.7626064639466044E-5 0.0 1.9665337852957507 2.486345817551944E-4 52 0.0 2.7626064639466044E-5 0.0 2.0809885710970586 2.486345817551944E-4 53 0.0 2.7626064639466044E-5 0.0 2.2097536583816098 2.762606463946604E-4 54 2.7626064639466044E-5 2.7626064639466044E-5 0.0 2.3426902814267203 2.762606463946604E-4 55 2.7626064639466044E-5 2.7626064639466044E-5 0.0 2.4831964461830447 2.762606463946604E-4 56 2.7626064639466044E-5 2.7626064639466044E-5 0.0 2.6351674277647477 2.762606463946604E-4 57 2.7626064639466044E-5 2.7626064639466044E-5 0.0 2.7925531180157854 3.038867110341265E-4 58 2.7626064639466044E-5 2.7626064639466044E-5 0.0 2.949413913038674 3.038867110341265E-4 59 2.7626064639466044E-5 2.7626064639466044E-5 0.0 3.105142039411344 3.038867110341265E-4 60 2.7626064639466044E-5 2.7626064639466044E-5 0.0 3.2720587219629977 3.038867110341265E-4 61 2.7626064639466044E-5 2.7626064639466044E-5 0.0 3.4497495697240432 3.038867110341265E-4 62 2.7626064639466044E-5 2.7626064639466044E-5 0.0 3.635507228359813 3.3151277567359253E-4 63 2.7626064639466044E-5 2.7626064639466044E-5 0.0 3.83121027026579 3.3151277567359253E-4 64 2.7626064639466044E-5 2.7626064639466044E-5 0.0 4.038074242286112 3.3151277567359253E-4 65 2.7626064639466044E-5 2.7626064639466044E-5 0.0 4.2534194161507495 3.3151277567359253E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAA 430 0.0 23.11898 59 CGGTTAC 320 0.0 22.183346 4 CAATTCG 495 0.0 20.081211 54 CGATCGT 510 0.0 19.48601 1 TAGCGTC 480 0.0 19.225035 1 TCGCGCT 470 0.0 18.880228 37 CGTCGTG 455 0.0 18.72177 4 ACGATCG 420 0.0 18.59741 59 ATTCGCG 600 0.0 18.34102 56 CTCGCGC 505 0.0 18.27406 36 CCTCGCG 530 0.0 18.08002 35 TCGCGGA 545 0.0 17.588724 58 AATTCGC 555 0.0 17.27014 55 ATACGTA 300 4.5474735E-10 16.563107 1 CGCGCTT 580 0.0 16.524137 38 CGCTCCG 590 0.0 16.243843 25 CGCTATC 285 4.107278E-9 16.192188 39 GATCGCG 505 0.0 16.17043 61 AACTCGA 625 0.0 15.90322 13 GACGTAT 450 0.0 15.777004 39 >>END_MODULE