FastQCFastQC Report
Thu 26 May 2016
SRR936134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936134_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3619770
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70730.19539915519494333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51400.14199797224685545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13300.043.85836100-101
CGTACAT20850.036.969337100-101
GGTATCA83950.035.868381
GTATCAA151250.031.1216831
ACGTATC5400.030.312162100-101
GCGTACA8500.029.060774100-101
TCGAGTA5850.028.997927100-101
AGTATCA32150.027.215376100-101
CCGTACA10100.027.109058100-101
CGGTATC12300.027.099476100-101
CGAGTAC16700.027.087885100-101
TACCGTA3250.026.55306298-99
CCTGTCG10550.026.5130996-97
GCGTATC4300.026.300447100-101
TACGCAG11050.024.509085100-101
ACCGTAC3700.024.127998-99
TATCAAC195100.023.8474242
CGATACG1001.8244937E-623.8053796-97
CACGTAT5650.023.70085798-99
ATCAACG197350.023.4844443
Produced by FastQC (version 0.11.5)