FastQCFastQC Report
Thu 26 May 2016
SRR936133_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936133_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247802
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53920.23987877935868016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG2107.411254E-716.90385615
CGATCGT3900.016.3808211
GTAACGG2401.6751619E-716.26670329
AATTCGC3501.4551915E-1116.22842455
CGTAACG2008.737534E-615.97094628
CGCGCTT3803.6379788E-1215.87966938
CGCGGAA3602.1827873E-1115.77798559
TAACGGA2301.9189301E-615.43086530
TCGCGCT3805.2750693E-1114.94623537
ACGATCG3151.5394107E-814.65098659
TTGCGTA3705.693437E-1014.3898436
TGCGTAA3959.822543E-1114.37865637
CAATTCG4001.200533E-1014.20145154
ATTCGCG4051.4733814E-1014.02456456
GATTCGT3808.5128704E-1014.01084933
TGCGACT3051.6520244E-713.96366828
CGCTCCG4101.7826096E-1013.85291625
CTCGCGC3851.035005E-913.82919636
TATAGCG1800.001132637613.80450843
CCTCGCG4152.1827873E-1013.68479635
Produced by FastQC (version 0.11.5)