FastQCFastQC Report
Thu 26 May 2016
SRR936133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247802
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45600.20286484307781558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35000.15570766464306018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24140.10739380069952781No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA7750.039.936913100-101
CGGTATC8300.038.366184100-101
GGTATCA56950.034.6928441
GTATCAA98500.030.3897631
CGTACAT10800.030.311808100-101
CCGTACA5850.028.99759100-101
CCTGTCG7550.027.1933696-97
TCGGTAT2750.027.055164100-101
CCGGTAT4100.026.857199100-101
TAAATCG1950.024.41440296-97
ATACACG6300.024.08742596-97
ACCGTAC2600.024.0369498-99
GCGTATC2750.023.808544100-101
TATCAAC128000.023.1487732
CGGATAA2850.022.973158100-101
ATCAACG129700.022.7525983
TCAACGC130100.022.6821384
CCGTATA2650.022.460892100-101
TACGCAG6900.022.428339100-101
ACGTATC2700.022.04495100-101
Produced by FastQC (version 0.11.5)