FastQCFastQC Report
Thu 26 May 2016
SRR936132_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936132_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2252458
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53010.23534290095531193No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGC4200.020.28117236
CGCGCTT4500.019.72174638
CGGTTAC2755.456968E-1219.360164
CGATCGT4850.019.0270881
TCGCGGA4500.018.935458
ATTCGCG4500.018.93287756
CCTCGCG4700.018.87791335
CGCGGAA4350.018.77216559
AATTCGC4550.018.72357755
ATCGTTC5050.018.273543
TCGCGCT4700.018.12561437
TTGCGTA2951.8189894E-1118.04680636
GACGTAT4050.017.5312239
CAATTCG4900.017.38617754
CGCTCCG5200.017.06538225
CGTAATG1703.89335E-516.7025132
AGCGCGA4050.016.65355123
AACTCGA5950.016.10670913
ACGTATT4250.015.86985340
AAACTCG6350.015.65455712
Produced by FastQC (version 0.11.5)