FastQCFastQC Report
Thu 26 May 2016
SRR936132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2252458
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44730.19858305904039056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34130.1515233580381965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23490.10428607325863569No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA10000.046.727077100-101
CGTACAT10750.037.652985100-101
GGTATCA57450.035.4253771
CGGTATC8750.034.694534100-101
CCTGTCG8600.033.2206496-97
ATACACG6950.031.25886796-97
TAAATCG1350.030.86238196-97
GTATCAA100050.030.631521
CCGTATC5000.030.35772100-101
ACGTATC4050.030.129906100-101
TCGTATC4150.030.121054100-101
CCGGTAT3450.029.33113100-101
CACGTAT3400.028.01080198-99
CGCTTAA1303.6379788E-1127.47305100-101
CCGTACA6000.026.29018100-101
TCGAGTA3400.026.261002100-101
TACGCAG7500.026.190971100-101
TCGGTAT3200.026.042158100-101
ACCGTAC2700.025.35236798-99
TCGTACA5900.025.22243100-101
Produced by FastQC (version 0.11.5)