FastQCFastQC Report
Thu 26 May 2016
SRR936131_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936131_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235233
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64750.289678973064553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG703.1659616E-530.4342352
ATTCGCG1502.3646862E-1128.40210556
CGCGGAA1703.6379788E-1227.14785659
CGGTTAC1951.8189894E-1225.479944
CGCGATA700.001129780425.35618220
TCGCGCT2251.7644197E-1020.50895937
CGATCGT2601.8189894E-1220.4758681
ACGCGAA1054.966136E-420.28812854
CGGACTA900.004867589619.7188289
CTCGCGC2353.1468517E-1019.63447836
AACGCGA1106.793829E-419.36593853
TAGGCCG2701.0186341E-1018.4013563
CGCTCCG2559.367795E-1018.09654425
ATAACGC1401.6715098E-417.75449451
TCGCGGA2651.5606929E-917.41482558
TTGCGTA1854.160738E-617.2669136
CGCGCTT2501.436274E-817.03668638
CCTCGCG2301.03089405E-716.97495835
AATTCGC2551.8289938E-816.70637355
TTCGCGG3004.5474735E-1016.56641257
Produced by FastQC (version 0.11.5)