##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936131_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2235233 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.510501142386495 40.0 36.0 41.0 30.0 41.0 2 36.37078505909675 39.0 36.0 41.0 29.0 41.0 3 36.28435872233454 39.0 36.0 41.0 29.0 41.0 4 36.16523467575863 39.0 36.0 41.0 29.0 41.0 5 36.09121778355993 39.0 36.0 41.0 29.0 41.0 6 35.98649357807441 39.0 35.0 41.0 28.0 41.0 7 35.817725937296025 39.0 35.0 40.0 28.0 41.0 8 35.63024212688342 39.0 35.0 40.0 27.0 41.0 9 35.53935316810373 38.0 35.0 40.0 27.0 41.0 10-11 35.75018711695827 39.0 35.0 40.5 27.5 41.0 12-13 35.92769612832309 39.0 35.0 41.0 28.5 41.0 14-15 35.874343972194396 39.0 35.0 41.0 28.0 41.0 16-17 35.708412501068125 39.0 35.0 41.0 27.0 41.0 18-19 35.54945189159251 39.0 35.0 41.0 27.0 41.0 20-21 35.338424674295695 39.0 35.0 41.0 26.0 41.0 22-23 35.06441543230616 39.0 35.0 41.0 24.5 41.0 24-25 34.820690057815 39.0 34.0 41.0 24.0 41.0 26-27 33.88653531868937 38.0 33.0 40.0 22.5 40.5 28-29 33.91836488634518 38.0 33.0 40.0 22.5 40.5 30-31 34.28525840482849 38.0 34.0 40.0 22.5 41.0 32-33 34.132936924249066 38.0 34.0 40.0 22.0 41.0 34-35 33.86302076785731 38.0 33.0 40.0 20.5 41.0 36-37 33.5735030755183 37.5 33.0 40.0 19.5 41.0 38-39 33.2935613423746 37.0 33.0 40.0 18.5 41.0 40-41 32.912397275809724 36.5 32.0 40.0 17.0 41.0 42-43 32.54980487492803 36.0 32.0 39.0 13.0 41.0 44-45 32.18348221415843 35.5 32.0 39.0 10.0 41.0 46-47 31.798323038358866 35.0 31.0 38.5 8.0 40.0 48-49 31.336769365878187 35.0 31.0 37.5 6.5 40.0 50-51 30.90310540332932 35.0 31.0 37.0 2.0 39.0 52-53 30.45204280717044 35.0 30.0 36.5 2.0 39.0 54-55 30.003622888531083 34.5 30.0 36.0 2.0 38.5 56-57 29.568413002134452 34.0 29.5 35.5 2.0 37.0 58-59 29.147065652663503 34.0 29.0 35.0 2.0 37.0 60-61 28.801693380511114 34.0 29.0 35.0 2.0 36.5 62-63 28.46666768072948 34.0 29.0 35.0 2.0 36.0 64-65 28.10753241384679 34.0 28.5 35.0 2.0 36.0 66-67 27.73777073799465 34.0 27.0 35.0 2.0 35.0 68-69 27.396489314536783 34.0 26.5 35.0 2.0 35.0 70-71 26.99137875111901 33.0 26.0 35.0 2.0 35.0 72-73 26.52865003335223 33.0 25.0 35.0 2.0 35.0 74-75 25.967005453122784 33.0 24.5 35.0 2.0 35.0 76-77 24.29131907053985 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9123825551080458 1101 2 0.8923899587056567 1101 3 1.0743518162183605 1101 4 1.0220545809647348 1101 5 1.0263057588277888 1101 6 0.9207168446954981 1101 7 0.9662704264158464 1101 8 1.1705449970327493 1101 9 1.00132306242714 1101 10-11 0.9646731235443369 1101 12-13 1.1586135306798724 1101 14-15 1.077097003273309 1101 16-17 1.280868175435053 1101 18-19 1.3183295096469294 1101 20-21 1.2127044059444003 1101 22-23 1.2226072025139771 1101 24-25 1.2538858615195423 1101 26-27 0.9595602779236643 1101 28-29 1.1283398669348372 1101 30-31 1.260623771761054 1101 32-33 1.2831143070627107 1101 34-35 1.1653585341161872 1101 36-37 1.2817243545718355 1101 38-39 1.310891526483715 1101 40-41 1.1612115980515583 1101 42-43 1.0904140548380568 1101 44-45 1.0518893773762201 1101 46-47 1.0209409215863054 1101 48-49 1.0456248809850983 1101 50-51 1.097491449237129 1101 52-53 1.1356030757737976 1101 54-55 1.0831698166961061 1101 56-57 1.0337113614708109 1101 58-59 0.9836708568415169 1101 60-61 1.000221670332543 1101 62-63 0.7511886556306528 1101 64-65 0.7522601238373703 1101 66-67 0.7394599776609105 1101 68-69 0.8060007248276513 1101 70-71 0.990466525505898 1101 72-73 1.0482657007306848 1101 74-75 0.9925998196468697 1101 76-77 0.8642351930336041 1102 1 0.7417077756347297 1102 2 0.8562193563619545 1102 3 0.9150947255609623 1102 4 0.853062927175543 1102 5 0.7637572671015604 1102 6 0.8602591310904515 1102 7 0.7660217302597658 1102 8 0.7920550012179319 1102 9 0.7880701328025168 1102 10-11 0.823909288311583 1102 12-13 0.9666552094191729 1102 14-15 0.9838258842210905 1102 16-17 0.8458219372662299 1102 18-19 0.8693413971622164 1102 20-21 0.8913360539387369 1102 22-23 0.9535254064287315 1102 24-25 0.9146998182834309 1102 26-27 0.7984027542613106 1102 28-29 0.8538490300602035 1102 30-31 0.9220504144434258 1102 32-33 0.9500350019632293 1102 34-35 0.8629120158623707 1102 36-37 0.9135252688722773 1102 38-39 1.02838295491717 1102 40-41 0.9254930150562615 1102 42-43 0.9950468255868756 1102 44-45 1.0411346770993575 1102 46-47 0.8832540976535235 1102 48-49 0.8307014013315062 1102 50-51 0.9039239471445448 1102 52-53 0.859974896648179 1102 54-55 0.9491294297281065 1102 56-57 1.0490104004895464 1102 58-59 1.1126814847791913 1102 60-61 1.0433697491748575 1102 62-63 0.8747615702852123 1102 64-65 1.023683003883729 1102 66-67 0.8619362951871459 1102 68-69 0.856466325883602 1102 70-71 0.90637764681167 1102 72-73 0.9158360036731885 1102 74-75 0.9642930259761471 1102 76-77 1.175619480909468 1103 1 0.5922462160919224 1103 2 0.4905586887119071 1103 3 0.549716678697294 1103 4 0.7285553724851468 1103 5 0.8596218851957431 1103 6 0.878666761952438 1103 7 0.7851139986417053 1103 8 0.6685766698191173 1103 9 0.5459437161721183 1103 10-11 0.579507547649655 1103 12-13 0.6368235274778868 1103 14-15 0.7390123031084244 1103 16-17 0.7237118733465806 1103 18-19 0.9169332766257128 1103 20-21 0.812085104816525 1103 22-23 0.6923704496329393 1103 24-25 0.7760663880227483 1103 26-27 0.5124036167333017 1103 28-29 0.5095800103361938 1103 30-31 0.8512487437062148 1103 32-33 0.7803409931208805 1103 34-35 0.8314214607366992 1103 36-37 0.7116463696122253 1103 38-39 0.765426983306206 1103 40-41 0.852078739331219 1103 42-43 0.7653984189434269 1103 44-45 0.6960295079097634 1103 46-47 0.43756980774202603 1103 48-49 0.5640475558752023 1103 50-51 0.4111942535236075 1103 52-53 0.5014067009073386 1103 54-55 0.6408707566452918 1103 56-57 0.6936328977086816 1103 58-59 0.479469738837647 1103 60-61 0.5819637334327297 1103 62-63 0.6629079456862215 1103 64-65 0.5317580994004878 1103 66-67 0.6528277698127347 1103 68-69 0.5892808661940911 1103 70-71 0.773162752242829 1103 72-73 0.7708613639769055 1103 74-75 0.7160135618775492 1103 76-77 0.9135320266527209 1104 1 0.5711813753779538 1104 2 0.3098669450315086 1104 3 0.44927212485481505 1104 4 0.34821569157932686 1104 5 0.40444947140263565 1104 6 0.28901755670431584 1104 7 0.4398963089790726 1104 8 0.49734959678846735 1104 9 0.5473072135279224 1104 10-11 0.4982216980371419 1104 12-13 0.47911283058752474 1104 14-15 0.5096915157409114 1104 16-17 0.5011365938221601 1104 18-19 0.5446418104185113 1104 20-21 0.5660767657024977 1104 22-23 0.588282568200519 1104 24-25 0.4614647336260731 1104 26-27 0.2463866252290572 1104 28-29 0.22773138241134916 1104 30-31 0.34436449926126755 1104 32-33 0.46898435800559923 1104 34-35 0.44491881653642196 1104 36-37 0.49214828283471945 1104 38-39 0.6382293880739738 1104 40-41 0.5217338371554447 1104 42-43 0.6785795149625784 1104 44-45 0.5250989284233825 1104 46-47 0.5247474875588303 1104 48-49 0.566407196950184 1104 50-51 0.5054821841851442 1104 52-53 0.3358786440402497 1104 54-55 0.4575881442201606 1104 56-57 0.4983849992698417 1104 58-59 0.5340752718920108 1104 60-61 0.2148327466872928 1104 62-63 0.3796958204055798 1104 64-65 0.2960158958753851 1104 66-67 0.31592868204318947 1104 68-69 0.23177827420650132 1104 70-71 0.2970086651072883 1104 72-73 0.31874936027726264 1104 74-75 0.372648677155734 1104 76-77 0.39643124868349844 1105 1 0.37684669042123176 1105 2 0.30642893278062644 1105 3 0.22612341752441267 1105 4 0.40645052895315104 1105 5 0.35522018431208124 1105 6 0.20078099385383297 1105 7 0.23595071900984266 1105 8 0.4733036415933398 1105 9 0.3983065520951996 1105 10-11 0.4178001065724999 1105 12-13 0.44230481862421556 1105 14-15 0.14823363175045756 1105 16-17 0.3566746549615516 1105 18-19 0.5313125031973982 1105 20-21 0.47057594610108566 1105 22-23 0.4372458443055436 1105 24-25 0.48958065689987507 1105 26-27 0.3910398044198047 1105 28-29 0.5682836787332093 1105 30-31 0.45345919917212285 1105 32-33 0.5279050723529366 1105 34-35 0.6145367672453048 1105 36-37 0.49604282745266914 1105 38-39 0.45703277000823306 1105 40-41 0.6929054358095712 1105 42-43 0.4329241214101103 1105 44-45 0.33311017277963373 1105 46-47 0.2585682680494479 1105 48-49 0.2955138314079555 1105 50-51 0.42708515200500585 1105 52-53 0.36640592095049485 1105 54-55 0.3232806355208844 1105 56-57 0.6386863825269664 1105 58-59 0.5300362186035414 1105 60-61 0.17961433009971373 1105 62-63 0.21679213076131632 1105 64-65 0.4280256448185291 1105 66-67 0.5826320150889437 1105 68-69 0.34249132743745747 1105 70-71 -0.029862758123375244 1105 72-73 -0.003725692205986064 1105 74-75 0.0045354858866453185 1105 76-77 0.19639745809885056 1106 1 -0.1305374358139062 1106 2 -0.008407571598169739 1106 3 -0.42258288580616465 1106 4 -0.32184508326366057 1106 5 -0.33150776863623577 1106 6 -0.2724027470248487 1106 7 -0.05759147078101989 1106 8 -0.09092477479385508 1106 9 -0.06094453066656058 1106 10-11 -0.3847468411909176 1106 12-13 -0.25254665623492656 1106 14-15 -0.18328456594976217 1106 16-17 -0.1111844539999538 1106 18-19 -0.08522271534914694 1106 20-21 -0.16970279132286947 1106 22-23 -0.101704087442549 1106 24-25 -0.11681297404548019 1106 26-27 -0.09549870032974184 1106 28-29 -0.05873860003792686 1106 30-31 -0.06572717066895706 1106 32-33 -0.08841668343443132 1106 34-35 -0.13359894451256338 1106 36-37 -0.011002145780224737 1106 38-39 -0.1001668232890367 1106 40-41 -0.12846415248817067 1106 42-43 0.012140572647915349 1106 44-45 -0.2547866292111891 1106 46-47 -0.1426349878193811 1106 48-49 -0.2580913018096602 1106 50-51 -0.3480613227698761 1106 52-53 -0.22786386788989788 1106 54-55 -0.2651161187216857 1106 56-57 -0.24474048119953196 1106 58-59 -0.3567976231082035 1106 60-61 -0.36891614732660116 1106 62-63 -0.46980521668303155 1106 64-65 -0.3604503153425789 1106 66-67 -0.3597534031576295 1106 68-69 -0.4129905928285815 1106 70-71 -0.4962451822738778 1106 72-73 -0.5316422675892376 1106 74-75 -0.7525003590045749 1106 76-77 -0.5235555520069255 1107 1 -0.042274116678498785 1107 2 -0.14485803601122882 1107 3 -0.05271653777996477 1107 4 -0.168434329689795 1107 5 -0.09403256081545663 1107 6 -0.17267722218931425 1107 7 -0.3374604018903682 1107 8 -0.10239239954955082 1107 9 -0.1688004452044396 1107 10-11 -0.12067152173759155 1107 12-13 -0.025468583901371744 1107 14-15 -0.023335496040331805 1107 16-17 -0.024859124611694483 1107 18-19 -0.010637092464833131 1107 20-21 -0.1970532163574532 1107 22-23 -0.17639312397059115 1107 24-25 -0.4133567907224389 1107 26-27 -0.4722007353657389 1107 28-29 -0.31233640134644247 1107 30-31 -0.25670959125269377 1107 32-33 -0.3035784783701416 1107 34-35 -0.2439821710905008 1107 36-37 -0.16128136261831116 1107 38-39 -0.2599366127767908 1107 40-41 -0.1975124037589424 1107 42-43 -0.13182408969073123 1107 44-45 -0.19072390906205072 1107 46-47 -0.28479194353846893 1107 48-49 -0.09019270317743278 1107 50-51 -0.1463837186427206 1107 52-53 -0.15010200106138072 1107 54-55 -0.189447587899906 1107 56-57 -0.11815405268229284 1107 58-59 -0.09801243085817646 1107 60-61 -0.02351570621547694 1107 62-63 -0.020162693715267466 1107 64-65 -0.1468672719287163 1107 66-67 -0.13546953135081097 1107 68-69 -0.19405307092656798 1107 70-71 -0.09324198683744811 1107 72-73 -0.1721897638049299 1107 74-75 -0.06363204747390583 1107 76-77 -0.11727947280201079 1108 1 0.2344997967723259 1108 2 0.17367302713225996 1108 3 0.21360930484146934 1108 4 0.27657569175845254 1108 5 0.37786681313681214 1108 6 0.2358988119940264 1108 7 0.09718770976094504 1108 8 0.25374628123302756 1108 9 0.28900198001949917 1108 10-11 0.0029505291292082347 1108 12-13 0.0699834101827932 1108 14-15 0.2251815722092374 1108 16-17 0.19852610889306987 1108 18-19 0.055092482351383865 1108 20-21 0.0834440248164725 1108 22-23 0.14187150918417046 1108 24-25 0.17812154856130036 1108 26-27 0.005432529819515253 1108 28-29 0.10054015582599618 1108 30-31 0.17522863045051196 1108 32-33 0.2280242172154061 1108 34-35 0.23524486406654432 1108 36-37 0.12305025362881139 1108 38-39 0.09928818052037514 1108 40-41 0.1041034920313777 1108 42-43 0.11189180141510491 1108 44-45 -0.14710844156734737 1108 46-47 -0.0939734890164381 1108 48-49 -0.12116215700013555 1108 50-51 -0.007790560999819007 1108 52-53 -0.08868821563079266 1108 54-55 -0.028169610659801947 1108 56-57 0.05596568271132085 1108 58-59 -0.062293245556748644 1108 60-61 -0.036210865136574455 1108 62-63 -0.027293983052377513 1108 64-65 -0.21834412221597788 1108 66-67 -0.370046706540446 1108 68-69 -0.2632157028031372 1108 70-71 -0.194154331789413 1108 72-73 -0.2496993502654341 1108 74-75 -0.3632501366062364 1108 76-77 -0.505576713306727 1109 1 0.24883792023985052 1109 2 0.2738470445340013 1109 3 -0.04872410091289936 1109 4 0.1598197403012378 1109 5 0.16261039094286645 1109 6 0.17455881592545808 1109 7 0.24496407360422268 1109 8 0.17790200715045046 1109 9 0.3278527616493818 1109 10-11 0.19333063610542922 1109 12-13 0.19024681583370295 1109 14-15 0.20924718566713807 1109 16-17 0.2809032776444269 1109 18-19 0.148467509509274 1109 20-21 0.2365828559409664 1109 22-23 0.04891217876836862 1109 24-25 -0.0025942816424091575 1109 26-27 -0.1375214207479516 1109 28-29 0.05334812627822316 1109 30-31 0.31882495560027024 1109 32-33 0.17337447637925152 1109 34-35 0.14115659317048568 1109 36-37 0.23316560999203517 1109 38-39 0.249534929333187 1109 40-41 0.32892031854161274 1109 42-43 0.3533144609224337 1109 44-45 0.09479012759991434 1109 46-47 -0.007767523592310255 1109 48-49 0.0921834879091854 1109 50-51 0.24484742693690364 1109 52-53 0.3264241921617135 1109 54-55 0.35503367518602147 1109 56-57 0.26075933449029165 1109 58-59 0.357080933240443 1109 60-61 0.21320811124382288 1109 62-63 0.23304287821995473 1109 64-65 0.20481157266385708 1109 66-67 0.348200083655815 1109 68-69 0.4213648242150789 1109 70-71 0.5702442115131916 1109 72-73 0.5309813040068896 1109 74-75 0.5140245563802992 1109 76-77 0.374741421955072 1110 1 -0.5790085680746628 1110 2 -0.5911150103404879 1110 3 -0.5948579254192055 1110 4 -0.6194024349508922 1110 5 -0.6687262437170389 1110 6 -0.7534191215506425 1110 7 -0.7061867607859327 1110 8 -0.6158218489600245 1110 9 -0.675422395868182 1110 10-11 -0.5001446140103241 1110 12-13 -0.5093661841461667 1110 14-15 -0.5920367082878073 1110 16-17 -0.8168357162561719 1110 18-19 -0.5612266145320319 1110 20-21 -0.8587372116959742 1110 22-23 -0.920117855251398 1110 24-25 -0.7880442886879209 1110 26-27 -0.6304960367674468 1110 28-29 -0.6346822499522276 1110 30-31 -0.554956975675772 1110 32-33 -0.5311979615699016 1110 34-35 -0.4078662946850571 1110 36-37 -0.45619254368069306 1110 38-39 -0.45685887464048136 1110 40-41 -0.5008639880791534 1110 42-43 -0.5398748419742745 1110 44-45 -0.5802373499047633 1110 46-47 -0.4850182633712805 1110 48-49 -0.40918485156381124 1110 50-51 -0.5839748650694574 1110 52-53 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-0.5135092171053515 2314 22-23 -0.3115866139950114 2314 24-25 -0.3562920814111621 2314 26-27 -0.5381178126407704 2314 28-29 -0.43373716243836924 2314 30-31 -0.6036388273011752 2314 32-33 -0.8076671477790853 2314 34-35 -0.8136036598390959 2314 36-37 -0.7756926162202973 2314 38-39 -0.7592285883162688 2314 40-41 -0.6580197617141152 2314 42-43 -0.5808875726761471 2314 44-45 -0.6397502723184694 2314 46-47 -0.5961178602582926 2314 48-49 -0.4727603160763998 2314 50-51 -0.5484673727638381 2314 52-53 -0.4889824425386884 2314 54-55 -0.4335592990369257 2314 56-57 -0.25007756954289917 2314 58-59 -0.3554556239247617 2314 60-61 -0.24208698159437247 2314 62-63 -0.2661571999953729 2314 64-65 -0.2303969732111355 2314 66-67 -0.3294214901726562 2314 68-69 -0.13236145613586814 2314 70-71 -0.16591966660692137 2314 72-73 -0.15054414998209253 2314 74-75 -0.11256891093929511 2314 76-77 0.01899667598486232 2315 1 -1.0783049736376 2315 2 -1.0369359532288627 2315 3 -0.9474044475404781 2315 4 -0.9220270544222089 2315 5 -0.9387272623781229 2315 6 -0.8969040656091707 2315 7 -1.0991111147523824 2315 8 -1.0781020042666611 2315 9 -1.0906306141123991 2315 10-11 -1.14853055857688 2315 12-13 -1.103129203091953 2315 14-15 -1.453778605687745 2315 16-17 -1.2247001063459066 2315 18-19 -1.0294285536448342 2315 20-21 -1.1394715553321504 2315 22-23 -1.0226031597592353 2315 24-25 -1.2185640931572337 2315 26-27 -0.9922039787977894 2315 28-29 -0.9960841039154076 2315 30-31 -1.0809006737550746 2315 32-33 -1.0341248164799168 2315 34-35 -1.0721407402657022 2315 36-37 -1.1820762321741256 2315 38-39 -1.0922596655393946 2315 40-41 -1.1003781977483378 2315 42-43 -1.3217705245490343 2315 44-45 -1.0862219837940543 2315 46-47 -1.2142994477868108 2315 48-49 -1.1764901730232147 2315 50-51 -1.0097286742742355 2315 52-53 -0.9624245825560749 2315 54-55 -1.0523131323945272 2315 56-57 -1.3774283278306747 2315 58-59 -1.194049206387728 2315 60-61 -1.2197209946730183 2315 62-63 -1.1929454475276877 2315 64-65 -1.3736725745242957 2315 66-67 -1.3333077977502406 2315 68-69 -1.2382368626372404 2315 70-71 -1.2929353508309127 2315 72-73 -2.7532057411729802 2315 74-75 -3.4333893652942074 2315 76-77 -3.0879683417313153 2316 1 -3.1994179103510803 2316 2 -3.1763327618283768 2316 3 -2.6119920934726366 2316 4 -2.4870024087901967 2316 5 -3.191539463590445 2316 6 -2.7426663474264785 2316 7 -2.8553659688434294 2316 8 -2.754616712742717 2316 9 -2.5491730408020885 2316 10-11 -2.550703798959205 2316 12-13 -2.6405110417520277 2316 14-15 -2.794474376788145 2316 16-17 -3.848229645520547 2316 18-19 -3.800037283737261 2316 20-21 -3.142202377998082 2316 22-23 -3.433545015870827 2316 24-25 -3.416182300910787 2316 26-27 -3.116683914190034 2316 28-29 -3.361878658669557 2316 30-31 -3.480492543326619 2316 32-33 -3.49066758972463 2316 34-35 -3.423648099918651 2316 36-37 -3.331308086769546 2316 38-39 -3.625254024737494 2316 40-41 -3.413907458441262 2316 42-43 -3.705484604275256 2316 44-45 -3.3906841891836024 2316 46-47 -3.0028912895101776 2316 48-49 -3.2363518294943567 2316 50-51 -3.4872306004394495 2316 52-53 -4.806313920109954 2316 54-55 -3.7681565761613314 2316 56-57 -3.725862022880815 2316 58-59 -3.7137684968163107 2316 60-61 -3.517930821364562 2316 62-63 -3.5791324449068647 2316 64-65 -3.6011053443962844 2316 66-67 -3.6130952852572378 2316 68-69 -3.5776765264788644 2316 70-71 -3.3774602764917674 2316 72-73 -3.5571357370576386 2316 74-75 -3.6254035926157435 2316 76-77 -3.567721019023608 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 60365.0 3 5143.0 4 6608.0 5 7684.0 6 8732.0 7 9365.0 8 10825.0 9 11511.0 10 13962.0 11 15993.0 12 12515.0 13 10489.0 14 10458.0 15 10816.0 16 11101.0 17 11591.0 18 12052.0 19 12797.0 20 13653.0 21 14643.0 22 16044.0 23 18291.0 24 21127.0 25 25038.0 26 29332.0 27 34490.0 28 40809.0 29 48387.0 30 57433.0 31 69291.0 32 85070.0 33 106890.0 34 142923.0 35 191646.0 36 271528.0 37 371952.0 38 339716.0 39 94081.0 40 882.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.696248253386184 26.41578066423002 28.66911942028752 22.218851662096277 2 22.480442567996864 26.72862463511586 28.52910750815676 22.261825288730517 3 22.57013316318609 26.793850537400104 28.125303421543524 22.510712877870283 4 22.339692004254328 26.700019374459988 28.532091578105696 22.42819704317999 5 22.640487092914725 26.735970977738443 28.424223279554926 22.1993186497919 6 22.74800259683664 26.589745895687166 28.4166589486391 22.245592558837096 7 22.565974518519578 26.35611429348716 28.553549679221863 22.524361508771396 8 22.56521363654872 26.750242857788475 28.227793669991723 22.45674983567108 9 22.446174312135966 26.547965347161533 28.479367546769296 22.526492793933205 10-11 22.752353596856235 26.696739844932658 28.21553080830206 22.33537574990905 12-13 22.865161877786804 26.50045975931432 28.133063965832388 22.50131439706649 14-15 22.607451837411936 26.658484930714373 28.27490722282785 22.459156009045845 16-17 22.773461530717935 26.53323460524049 28.25629335665822 22.437010507383356 18-19 22.64923447214453 26.453795936649332 28.15784015834758 22.739129432858554 20-21 22.698475879408342 26.671584556037427 28.149658667601017 22.480280896953214 22-23 22.84200097817804 26.817292362022528 27.67331001397461 22.667396645824823 24-25 22.831723059079582 26.88004292075947 27.54049997852172 22.747734041639223 26-27 22.840500043854075 27.005496079087692 27.499570409060077 22.65443346799816 28-29 22.964800681861654 26.993108678412074 27.41578236801514 22.626308271711128 30-31 22.79268015229213 26.916777794108533 27.481889079265244 22.80865297433409 32-33 22.81898503044515 27.198822551916034 27.332849217652054 22.649343199986756 34-35 22.894071345732574 26.91104960411208 27.48333762868147 22.71154142147388 36-37 22.841951299831496 26.971380530134027 27.727963804630196 22.458704365404287 38-39 22.750446098583758 26.949097332715265 27.764625500910096 22.535831067790877 40-41 22.615831977245467 26.928750688483984 27.6601656477924 22.795251686478153 42-43 22.653309785704344 27.08372578138738 27.457903094675697 22.805061338232576 44-45 22.695404836914864 26.920982560134803 27.638027380637574 22.74558522231275 46-47 22.651783137220683 26.861571093912566 27.650474227911314 22.836171540955437 48-49 22.679310497589622 26.974604792651224 27.483573953344344 22.862510756414807 50-51 22.59073242951487 27.156534364018164 27.405239593048965 22.847493613418 52-53 22.641995421415512 26.96972002604825 27.52749724188398 22.86078731065226 54-55 22.63120150810832 26.939737199818758 27.492769918385385 22.93629137368754 56-57 22.541842700933003 27.14961303598113 27.43116345160584 22.877380811480023 58-59 22.6459024880368 27.179221920515 27.336926525404447 22.837949066043752 60-61 22.638896812798475 26.9874339845109 27.323549449111162 23.050119753579462 62-63 22.58554598804464 26.98217077783237 27.448844246471253 22.983438987651734 64-65 22.583934266230894 26.94862127209602 27.418784279689373 23.048660181983717 66-67 22.575669158988916 27.020991713938447 27.510699920701548 22.892639206371086 68-69 22.595011464527996 26.8312921863524 27.563876196524788 23.009820152594816 70-71 22.506126888704365 26.747522946457146 27.62677344052498 23.11957672431351 72-73 22.58989822732358 26.708045609526692 27.559087907976743 23.14296825517298 74-75 22.47920776828404 26.656908250891288 27.487701978161326 23.37618200266334 76-77 22.421294647165066 26.591889687462288 27.759352701045596 23.227462964327046 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6566.0 1 3963.5 2 1292.0 3 1261.5 4 1300.0 5 1367.0 6 1476.0 7 1617.5 8 1698.0 9 1679.0 10 1734.0 11 1872.0 12 2030.5 13 2106.0 14 2245.0 15 2490.0 16 2798.5 17 3001.0 18 3298.0 19 4000.5 20 4860.5 21 6162.0 22 7009.0 23 8210.0 24 10775.5 25 14169.5 26 16199.0 27 18435.0 28 24205.0 29 30191.0 30 32643.0 31 37001.0 32 45674.0 33 53758.0 34 61066.5 35 64606.0 36 69879.5 37 78910.0 38 83699.0 39 84731.0 40 87336.0 41 92622.5 42 98330.5 43 101357.0 44 102261.0 45 104491.5 46 106926.5 47 109097.0 48 110159.0 49 109870.5 50 108149.5 51 103316.5 52 99916.0 53 97888.0 54 91775.5 55 82617.5 56 72846.0 57 68148.0 58 63962.0 59 53978.0 60 43572.0 61 38964.0 62 34241.5 63 24933.0 64 17524.5 65 14702.0 66 12689.0 67 9530.5 68 7078.0 69 4865.0 70 3959.0 71 3354.5 72 2324.5 73 1732.0 74 1565.0 75 1398.5 76 962.5 77 561.0 78 429.0 79 361.5 80 248.5 81 171.5 82 124.5 83 109.0 84 92.0 85 74.5 86 64.0 87 54.0 88 51.0 89 43.5 90 42.0 91 45.0 92 37.0 93 30.0 94 29.0 95 23.0 96 19.0 97 21.0 98 33.5 99 75.0 100 106.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008679184675602051 2 0.012392444098668909 3 0.0136898479934754 4 0.014853037692267427 5 0.01158715892258212 6 0.00921604145965991 7 0.01588201319504499 8 0.01726889322052779 9 0.017000464828498863 10-11 0.020668986186227565 12-13 0.016463608044441004 14-15 0.007762054336169875 16-17 0.0061738530166653765 18-19 0.018007071298607347 20-21 0.023912495923243796 22-23 0.020847938447580185 24-25 0.018655773246010594 26-27 0.024561197870647044 28-29 0.007851530466846186 30-31 0.008343649185565889 32-33 0.010289755027775628 34-35 0.01149768279190581 36-37 0.011229254399876881 38-39 0.013466157666784627 40-41 0.011184516334538727 42-43 0.014763561561591119 44-45 0.013242467340093851 46-47 0.024516459805308886 48-49 0.023263793975840552 50-51 0.038519474256151376 52-53 0.0410695439804262 54-55 0.02887842117577899 56-57 0.020780831349572954 58-59 0.01603859642372853 60-61 0.01948342745476646 62-63 0.02626124435349693 64-65 0.02605992305947523 66-67 0.028498147620404674 68-69 0.042501162071247156 70-71 0.054938344235254226 72-73 0.0882010958141724 74-75 0.0871050132133876 76-77 0.09012483262371306 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2235233.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.408170250225865 #Duplication Level Percentage of deduplicated Percentage of total 1 78.96927467080984 47.7038938885113 2 11.936100052072735 14.420759281386793 3 3.8746826958494145 7.021874758694266 4 1.751355875890158 4.231848156780244 5 0.934624059287564 2.822946464670018 6 0.5730939738362152 2.0771735004525946 7 0.3705116386913638 1.5667351104830654 8 0.26498539852349895 1.2805826454265181 9 0.1959405517521585 1.0652769188250826 >10 1.0304911072418992 11.264740835325071 >50 0.07014375776939781 2.8948873577493344 >100 0.027755904651784756 2.900501464114617 >500 8.916973920572341E-4 0.3720331796961918 >1k 7.430811600473973E-5 0.086095516247235 >5k 7.430811600473973E-5 0.29065092163756334 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6475 0.289678973064553 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 4.47380653381549E-5 0.0 0.0 0.0 2 0.0 4.47380653381549E-5 0.0 1.789522613526196E-4 0.0 3 0.0 4.47380653381549E-5 0.0 2.2369032669077452E-4 0.0 4 4.47380653381549E-5 4.47380653381549E-5 0.0 6.263329147341687E-4 0.0 5 2.2369032669077452E-4 4.47380653381549E-5 0.0 0.0017447845481880413 0.0 6 2.2369032669077452E-4 4.47380653381549E-5 0.0 0.003310616835023463 0.0 7 2.2369032669077452E-4 4.47380653381549E-5 0.0 0.00702387625809032 4.47380653381549E-5 8 2.2369032669077452E-4 4.47380653381549E-5 0.0 0.010468707289128248 8.94761306763098E-5 9 2.2369032669077452E-4 4.47380653381549E-5 0.0 0.01422670477753326 8.94761306763098E-5 10 2.2369032669077452E-4 4.47380653381549E-5 0.0 0.018566297115334286 1.789522613526196E-4 11 3.1316645736708434E-4 4.47380653381549E-5 0.0 0.023755912694560254 1.789522613526196E-4 12 3.579045227052392E-4 4.47380653381549E-5 0.0 0.03001924184190194 1.789522613526196E-4 13 3.579045227052392E-4 1.342141960144647E-4 0.0 0.03914580717088554 1.789522613526196E-4 14 4.0264258804339413E-4 1.342141960144647E-4 0.0 0.05153825126955445 2.2369032669077452E-4 15 4.0264258804339413E-4 2.2369032669077452E-4 0.0 0.06531757539370615 2.2369032669077452E-4 16 4.0264258804339413E-4 2.2369032669077452E-4 0.0 0.08012587502063544 2.2369032669077452E-4 17 4.0264258804339413E-4 3.1316645736708434E-4 0.0 0.09296569977268589 2.2369032669077452E-4 18 4.0264258804339413E-4 3.1316645736708434E-4 0.0 0.10732661874623362 2.2369032669077452E-4 19 5.368567840578588E-4 3.1316645736708434E-4 0.0 0.12186648998113396 2.2369032669077452E-4 20 6.710709800723236E-4 3.1316645736708434E-4 0.0 0.1377037651108408 2.2369032669077452E-4 21 6.710709800723236E-4 3.1316645736708434E-4 0.0 0.1554647770500883 2.2369032669077452E-4 22 8.052851760867883E-4 3.1316645736708434E-4 0.0 0.17532847806022908 2.2369032669077452E-4 23 9.39499372101253E-4 3.1316645736708434E-4 0.0 0.19595272618111848 2.2369032669077452E-4 24 9.39499372101253E-4 3.579045227052392E-4 0.0 0.21841123498087223 2.2369032669077452E-4 25 9.39499372101253E-4 3.579045227052392E-4 0.0 0.2405565773232589 2.2369032669077452E-4 26 9.39499372101253E-4 3.579045227052392E-4 0.0 0.2651625132592441 2.2369032669077452E-4 27 9.39499372101253E-4 3.579045227052392E-4 0.0 0.29155797180875553 2.2369032669077452E-4 28 9.39499372101253E-4 3.579045227052392E-4 0.0 0.3169691929208275 2.2369032669077452E-4 29 9.39499372101253E-4 3.579045227052392E-4 0.0 0.3413067004647838 2.684283920289294E-4 30 9.39499372101253E-4 3.579045227052392E-4 0.0 0.3675679448182807 2.684283920289294E-4 31 9.39499372101253E-4 3.579045227052392E-4 0.0 0.39740823439883 2.684283920289294E-4 32 0.0011184516334538726 3.579045227052392E-4 0.0 0.42993280789966865 2.684283920289294E-4 33 0.0011184516334538726 3.579045227052392E-4 0.0 0.46549956984350177 2.684283920289294E-4 34 0.0011631896987920275 3.579045227052392E-4 0.0 0.5030348066622138 2.684283920289294E-4 35 0.0011631896987920275 3.579045227052392E-4 0.0 0.5415542809183651 2.684283920289294E-4 36 0.0011631896987920275 3.579045227052392E-4 0.0 0.5863818223871963 2.684283920289294E-4 37 0.0011631896987920275 3.579045227052392E-4 0.0 0.6316567445094091 2.684283920289294E-4 38 0.0011631896987920275 3.579045227052392E-4 0.0 0.6776027376116942 2.684283920289294E-4 39 0.0011631896987920275 3.579045227052392E-4 0.0 0.7239066352366845 2.684283920289294E-4 40 0.0012079277641301824 3.579045227052392E-4 0.0 0.7703894851230274 2.684283920289294E-4 41 0.0012079277641301824 3.579045227052392E-4 0.0 0.8217935221965674 2.684283920289294E-4 42 0.0013421419601446472 3.579045227052392E-4 0.0 0.8766871283664835 2.684283920289294E-4 43 0.0013421419601446472 3.579045227052392E-4 0.0 0.9371729927036689 3.1316645736708434E-4 44 0.0013421419601446472 3.579045227052392E-4 0.0 1.0030721629467712 3.1316645736708434E-4 45 0.0013421419601446472 3.579045227052392E-4 0.0 1.072639854547602 3.1316645736708434E-4 46 0.0013421419601446472 3.579045227052392E-4 0.0 1.15124463534674 3.1316645736708434E-4 47 0.0014316180908209568 3.579045227052392E-4 0.0 1.2281941077283667 3.1316645736708434E-4 48 0.0014316180908209568 3.579045227052392E-4 0.0 1.307201531115548 4.0264258804339413E-4 49 0.0014763561561591117 3.579045227052392E-4 0.0 1.3896985235991057 4.0264258804339413E-4 50 0.0014763561561591117 3.579045227052392E-4 0.0 1.471166540579886 4.0264258804339413E-4 51 0.0014763561561591117 3.579045227052392E-4 0.0 1.5588084105773312 4.0264258804339413E-4 52 0.0014763561561591117 3.579045227052392E-4 0.0 1.652310967134075 4.0264258804339413E-4 53 0.0014763561561591117 3.579045227052392E-4 0.0 1.7539111135170249 4.4738065338154905E-4 54 0.0014763561561591117 3.579045227052392E-4 0.0 1.8615061606552874 4.4738065338154905E-4 55 0.0015210942214972667 3.579045227052392E-4 0.0 1.9796146531480163 4.4738065338154905E-4 56 0.0017447845481880413 3.579045227052392E-4 0.0 2.1052838786828936 4.4738065338154905E-4 57 0.0017895226135261962 3.579045227052392E-4 0.0 2.230147819041684 5.368567840578588E-4 58 0.001923736809540661 3.579045227052392E-4 0.0 2.366241013800351 5.815948493960138E-4 59 0.001923736809540661 3.579045227052392E-4 0.0 2.4947734755168702 5.815948493960138E-4 60 0.001923736809540661 3.579045227052392E-4 0.0 2.6284060766819386 5.815948493960138E-4 61 0.0021474271362314354 3.579045227052392E-4 0.0 2.767988840536982 5.815948493960138E-4 62 0.0021474271362314354 3.579045227052392E-4 0.0 2.913253338689971 5.815948493960138E-4 63 0.0021921652015695903 3.579045227052392E-4 0.0 3.063796928552862 6.263329147341687E-4 64 0.0021921652015695903 3.579045227052392E-4 0.0 3.226375057991717 6.263329147341687E-4 65 0.0021921652015695903 3.579045227052392E-4 0.0 3.4011219412025504 6.263329147341687E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCG 70 3.1659616E-5 30.43423 52 ATTCGCG 150 2.3646862E-11 28.402105 56 CGCGGAA 170 3.6379788E-12 27.147856 59 CGGTTAC 195 1.8189894E-12 25.47994 4 CGCGATA 70 0.0011297804 25.356182 20 TCGCGCT 225 1.7644197E-10 20.508959 37 CGATCGT 260 1.8189894E-12 20.475868 1 ACGCGAA 105 4.966136E-4 20.288128 54 CGGACTA 90 0.0048675896 19.718828 9 CTCGCGC 235 3.1468517E-10 19.634478 36 AACGCGA 110 6.793829E-4 19.365938 53 TAGGCCG 270 1.0186341E-10 18.401356 3 CGCTCCG 255 9.367795E-10 18.096544 25 ATAACGC 140 1.6715098E-4 17.754494 51 TCGCGGA 265 1.5606929E-9 17.414825 58 TTGCGTA 185 4.160738E-6 17.26691 36 CGCGCTT 250 1.436274E-8 17.036686 38 CCTCGCG 230 1.03089405E-7 16.974958 35 AATTCGC 255 1.8289938E-8 16.706373 55 TTCGCGG 300 4.5474735E-10 16.566412 57 >>END_MODULE