FastQCFastQC Report
Thu 26 May 2016
SRR936131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936131_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235233
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61290.2741996024575514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45830.20503455344476393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31300.14003014450842483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA9750.047.934734100-101
CGGTATC7900.042.95691100-101
CGTACAT14900.038.75884100-101
GGTATCA67650.034.211771
TACGCAG7950.033.700058100-101
GCGTATC3150.032.130955100-101
TCGGTAT3050.031.232405100-101
GTATCAA129050.030.336231
CGAGTAC10300.029.47937100-101
ACGTATC4250.028.017302100-101
CCGTACA7700.027.835373100-101
TCGTATC4500.027.783825100-101
TCGTACA9100.027.47851100-101
AGTATCA28050.026.531536100-101
CCGTATC4600.025.885551100-101
GCGTACA5550.025.74563100-101
TCGAGTA4950.025.258022100-101
CCGGTAT3700.024.94108100-101
CTACGCG1201.2993041E-824.80004796-97
CCTGTCG4950.024.64974496-97
Produced by FastQC (version 0.11.5)