FastQCFastQC Report
Thu 26 May 2016
SRR936130_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936130_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2242801
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67240.29980368298391163No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG500.00673581328.39435436
CGCGGAA1701.1641532E-1025.06110659
ATTCGCG1751.6916601E-1024.34127456
TCGCGCT1956.548362E-1021.84229737
CTCGCGC1956.566552E-1021.84180836
GCGTAAG1901.153785E-820.55037738
TAACGCG1459.883428E-619.58799252
TCGCGGA2253.8835424E-918.93463758
AATTCGC2253.8889993E-918.93294755
CGCTCCG2455.493348E-1018.83467125
TAGGCCG3300.018.2842433
CGCGCTT2356.675691E-918.12567338
CGGTTTA1000.008928237517.75161660
ACGACCG1200.00122081117.7480558
TGCGTAA2401.6736885E-716.26796237
CAATTCG2652.9398507E-816.07514454
CGATCGT2652.9454895E-816.0726341
ACGAATC2754.6244168E-815.49370448
CCTCGCG2805.7980287E-815.21092135
TATACGA1400.00342268415.21058232
Produced by FastQC (version 0.11.5)