##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936130_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2242801 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.491290578165426 39.0 36.0 41.0 30.0 41.0 2 36.35452097622571 39.0 36.0 41.0 29.0 41.0 3 36.22460887078256 39.0 36.0 41.0 29.0 41.0 4 36.12159393544055 39.0 36.0 41.0 29.0 41.0 5 36.046735309998525 39.0 35.0 41.0 28.0 41.0 6 35.949200129659296 39.0 35.0 41.0 28.0 41.0 7 35.802668627310226 39.0 35.0 40.0 27.0 41.0 8 35.671809491791734 39.0 35.0 40.0 27.0 41.0 9 35.82918725290385 39.0 35.0 40.0 28.0 41.0 10-11 36.04789479762137 39.0 35.0 41.0 29.0 41.0 12-13 35.97223405018992 39.0 35.0 41.0 28.0 41.0 14-15 35.820793061890015 39.0 35.0 41.0 27.5 41.0 16-17 35.66861549464264 39.0 35.0 41.0 27.0 41.0 18-19 35.50791688607237 39.0 35.0 41.0 26.5 41.0 20-21 35.29040316104728 39.0 35.0 41.0 25.5 41.0 22-23 35.0112678298253 39.0 34.5 41.0 24.5 41.0 24-25 34.769201324593666 39.0 34.0 41.0 24.0 41.0 26-27 33.81854029849282 38.0 33.0 40.0 22.5 40.5 28-29 33.82249383694764 38.0 33.0 39.5 22.0 40.5 30-31 34.22616473775426 38.0 34.0 40.0 22.0 41.0 32-33 34.10883466700791 38.0 34.0 40.0 22.0 41.0 34-35 33.85833718640218 38.0 33.0 40.0 20.5 41.0 36-37 33.54698009319596 37.5 33.0 40.0 19.0 41.0 38-39 33.2401120295559 37.0 33.0 40.0 18.0 41.0 40-41 32.87887601262885 36.5 32.0 40.0 15.5 41.0 42-43 32.51008760919939 36.0 32.0 39.0 13.0 41.0 44-45 32.1552204141161 35.5 31.5 39.0 9.5 41.0 46-47 31.738282620705093 35.0 31.0 38.5 7.5 40.0 48-49 31.31955131106148 35.0 31.0 37.5 4.5 40.0 50-51 30.86884948776106 35.0 31.0 37.0 2.0 39.0 52-53 30.39090851127675 35.0 30.0 36.5 2.0 39.0 54-55 29.93702807337789 34.5 30.0 36.0 2.0 38.5 56-57 29.510509180261643 34.0 29.0 35.5 2.0 37.0 58-59 29.094063405536204 34.0 29.0 35.0 2.0 37.0 60-61 28.739985179246844 34.0 29.0 35.0 2.0 36.5 62-63 28.38008499193642 34.0 29.0 35.0 2.0 36.0 64-65 28.01325797518371 34.0 27.5 35.0 2.0 36.0 66-67 27.67968870176177 34.0 27.0 35.0 2.0 35.0 68-69 27.32874896167783 34.0 26.5 35.0 2.0 35.0 70-71 26.895458402238987 33.0 25.5 35.0 2.0 35.0 72-73 26.437596336010195 33.0 25.0 35.0 2.0 35.0 74-75 25.88798961655537 33.0 24.0 35.0 2.0 35.0 76-77 24.15477320546941 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9536308285045152 1101 2 0.7082080284504002 1101 3 0.80119992014275 1101 4 0.7299198133915823 1101 5 0.8169728772063323 1101 6 0.8513903129848543 1101 7 0.9375568718607781 1101 8 0.9930575875673 1101 9 0.9294985443038115 1101 10-11 0.9850773315320183 1101 12-13 1.125411945113818 1101 14-15 1.2127149304197786 1101 16-17 1.1179397348483988 1101 18-19 1.1582528436133188 1101 20-21 1.0322599695895391 1101 22-23 1.0553801535961824 1101 24-25 1.0652933210301327 1101 26-27 1.0867618601070532 1101 28-29 1.2152047811301614 1101 30-31 1.3002257100084833 1101 32-33 1.2825941855976168 1101 34-35 1.2806507541516723 1101 36-37 1.294721372577122 1101 38-39 1.3149428017185514 1101 40-41 1.1792249800329841 1101 42-43 1.066368621057734 1101 44-45 1.120970262323958 1101 46-47 1.1938561962248961 1101 48-49 1.0697543281929605 1101 50-51 0.9891601598912771 1101 52-53 1.0973346290294401 1101 54-55 1.2267453379772029 1101 56-57 1.2292048711202703 1101 58-59 1.2228438553088488 1101 60-61 1.0445761933895987 1101 62-63 1.0967179643349532 1101 64-65 1.0676718372148173 1101 66-67 1.1505134083487825 1101 68-69 1.21020909311164 1101 70-71 1.0445056127314878 1101 72-73 0.9671372384931622 1101 74-75 1.0637319378181935 1101 76-77 1.2920417573684801 1102 1 1.0270138062718672 1102 2 1.0482115732926545 1102 3 0.8561671927610917 1102 4 0.7658994630014035 1102 5 0.8747617943319099 1102 6 1.0776154054752851 1102 7 0.9604183583978667 1102 8 0.9010504579409044 1102 9 1.0134493958148667 1102 10-11 0.9725946188789223 1102 12-13 1.16857476820622 1102 14-15 1.0179061272284216 1102 16-17 0.7837806862640875 1102 18-19 0.9065407097680733 1102 20-21 0.9789883547890739 1102 22-23 1.0233256678729106 1102 24-25 1.0499756083021552 1102 26-27 0.8844535936347739 1102 28-29 1.0244559456857303 1102 30-31 1.0009997088401903 1102 32-33 1.0932396963794275 1102 34-35 1.0219245757412594 1102 36-37 0.984746885455877 1102 38-39 1.0644540129062747 1102 40-41 1.0646253474983212 1102 42-43 1.0431233179737163 1102 44-45 0.9188738126582265 1102 46-47 0.9738049957216255 1102 48-49 0.9039852721694679 1102 50-51 0.9951820230803357 1102 52-53 0.9520975739445916 1102 54-55 0.9227015966208789 1102 56-57 0.9163634304164958 1102 58-59 1.0772565598237804 1102 60-61 1.040209422041908 1102 62-63 1.009111057384569 1102 64-65 0.9483596863942623 1102 66-67 0.9036673194081892 1102 68-69 1.0025990407378451 1102 70-71 1.0529377190168425 1102 72-73 0.9034779877030594 1102 74-75 0.93227308544839 1102 76-77 0.8781704401350581 1103 1 0.6755658871321444 1103 2 0.6748874151152222 1103 3 0.5661863906901772 1103 4 0.5509841303276843 1103 5 0.48983765206236285 1103 6 0.5542106834837099 1103 7 0.5428611110892518 1103 8 0.4534752660520027 1103 9 0.5771777228232082 1103 10-11 0.5743630458177265 1103 12-13 0.48306649309133576 1103 14-15 0.6864018797016769 1103 16-17 0.6324925690952625 1103 18-19 0.34023265350717224 1103 20-21 0.2801004608127471 1103 22-23 0.2653728685063328 1103 24-25 0.3707467311459993 1103 26-27 0.4224744112761414 1103 28-29 0.2494360306965291 1103 30-31 0.4161887295047748 1103 32-33 0.48803371935186135 1103 34-35 0.6279847581758204 1103 36-37 0.5210448305664315 1103 38-39 0.5209547353895445 1103 40-41 0.5593592338316355 1103 42-43 0.5668976573444198 1103 44-45 0.4388083251838779 1103 46-47 0.3534603730097423 1103 48-49 0.32898210866892086 1103 50-51 0.30459587764608287 1103 52-53 0.5472322908625102 1103 54-55 0.4691046663613001 1103 56-57 0.4592489285684067 1103 58-59 0.3935317759360615 1103 60-61 0.28995363042561095 1103 62-63 0.2977777714535286 1103 64-65 0.3043852209655995 1103 66-67 0.31274542111503223 1103 68-69 0.16466860882662004 1103 70-71 0.14706059781440217 1103 72-73 0.13222257811711202 1103 74-75 0.3328726441250005 1103 76-77 0.44187177193866134 1104 1 0.4624344865733292 1104 2 0.3736404221436658 1104 3 0.464976896381458 1104 4 0.5463165213988219 1104 5 0.3738324291669244 1104 6 0.31433688876057886 1104 7 0.3373183167844971 1104 8 0.4677720953497797 1104 9 0.5406167586063546 1104 10-11 0.546294971821915 1104 12-13 0.6551968417848144 1104 14-15 0.7197016668921208 1104 16-17 0.7697384872289064 1104 18-19 0.6906890999473134 1104 20-21 0.7648351867789671 1104 22-23 0.691779654279209 1104 24-25 0.586672431815245 1104 26-27 0.4602931818165743 1104 28-29 0.6031082891347097 1104 30-31 0.6968167949798598 1104 32-33 0.7499602549232662 1104 34-35 0.6697167555109971 1104 36-37 0.6611305298619641 1104 38-39 0.6947685791254727 1104 40-41 0.6891448672492615 1104 42-43 0.6935186449468915 1104 44-45 0.5720849773700891 1104 46-47 0.4979592425967745 1104 48-49 0.5152147159374891 1104 50-51 0.43034994071669885 1104 52-53 0.4576444814550342 1104 54-55 0.4537621209492819 1104 56-57 0.4843334687932668 1104 58-59 0.4320043365911914 1104 60-61 0.5012855128515206 1104 62-63 0.5582841843978095 1104 64-65 0.5033309423472865 1104 66-67 0.527334535782277 1104 68-69 0.3924993227839906 1104 70-71 0.24112260565895483 1104 72-73 0.15577828207909405 1104 74-75 0.07378472948457926 1104 76-77 0.15888495703470795 1105 1 0.005419864607929981 1105 2 0.17983875621320777 1105 3 0.01490004542273482 1105 4 0.17389023972315698 1105 5 -0.028332919840096338 1105 6 0.1583670792427796 1105 7 0.24782413582190088 1105 8 -0.020535290455555355 1105 9 0.14753221804436834 1105 10-11 0.24685839527295173 1105 12-13 0.2838142457098485 1105 14-15 0.15402790259243204 1105 16-17 0.19665874703472497 1105 18-19 0.1430118694665552 1105 20-21 0.16595696286997708 1105 22-23 0.1798586282174952 1105 24-25 0.3777373246746407 1105 26-27 0.13329483152044475 1105 28-29 0.2746496579381841 1105 30-31 0.4613694592966695 1105 32-33 0.48313564188249813 1105 34-35 0.45045353308945124 1105 36-37 0.4587759832600895 1105 38-39 0.3968958407072023 1105 40-41 0.5822174632340733 1105 42-43 0.7287675428906155 1105 44-45 0.37401684369900323 1105 46-47 0.4339903976599153 1105 48-49 0.3342939242599279 1105 50-51 0.28092632290710995 1105 52-53 0.2543987116383022 1105 54-55 0.018253822848695478 1105 56-57 0.12595423522945381 1105 58-59 0.09638842461145458 1105 60-61 0.2705637223368669 1105 62-63 0.13800578560217858 1105 64-65 0.18320038586521292 1105 66-67 0.21385857368039396 1105 68-69 -0.045121475203909966 1105 70-71 -0.1694047361542772 1105 72-73 -0.5729324247694301 1105 74-75 -0.5593100879211477 1105 76-77 -0.49316951023715205 1106 1 0.43129549324152094 1106 2 0.13556422612796126 1106 3 0.3486367148238827 1106 4 0.4018702304496955 1106 5 0.5984424127561141 1106 6 0.42616409746869977 1106 7 0.003727049828391671 1106 8 0.2255983900380798 1106 9 0.25344315716053956 1106 10-11 0.22056635261503033 1106 12-13 0.45925384338435293 1106 14-15 0.3655787104534767 1106 16-17 0.5171678064918765 1106 18-19 0.6375097651044612 1106 20-21 0.6572457063161394 1106 22-23 0.5193934393599164 1106 24-25 0.3826330874167283 1106 26-27 0.27486400796585286 1106 28-29 0.5242533066880455 1106 30-31 0.6889593405250451 1106 32-33 0.5510285155851022 1106 34-35 0.4365326428372853 1106 36-37 0.4568263274481197 1106 38-39 0.5497224626710349 1106 40-41 0.5532441422653562 1106 42-43 0.5455409137933529 1106 44-45 0.43543122469554163 1106 46-47 0.5325699532471795 1106 48-49 0.44416525500144743 1106 50-51 0.35512247442117584 1106 52-53 0.4403378278062249 1106 54-55 0.4401115465709218 1106 56-57 0.38914038210679536 1106 58-59 0.4118907073093183 1106 60-61 0.02971100984979458 1106 62-63 0.02335148949346788 1106 64-65 0.056837454738484894 1106 66-67 -0.08839024038673315 1106 68-69 -0.009217580350682653 1106 70-71 0.008156611350806031 1106 72-73 -0.03383409042553254 1106 74-75 -0.14761839480134142 1106 76-77 -0.22875552157930912 1107 1 0.22913851005738195 1107 2 0.38639370416083096 1107 3 0.2506051725392169 1107 4 0.22886578444744288 1107 5 0.1950948946394604 1107 6 0.018496912980872082 1107 7 0.10628073619891865 1107 8 -0.01958170453092123 1107 9 0.12289565061404062 1107 10-11 0.22375580199475564 1107 12-13 0.25957040623156047 1107 14-15 0.09907358640609942 1107 16-17 0.17763182075719186 1107 18-19 0.3518924037463975 1107 20-21 0.12693445212834575 1107 22-23 0.10453489301377772 1107 24-25 0.19910745697088572 1107 26-27 -0.15250157830922006 1107 28-29 -0.020068469534948008 1107 30-31 0.1987480320596049 1107 32-33 0.3506587385317772 1107 34-35 0.21424444048207647 1107 36-37 0.2724346368612913 1107 38-39 0.3415543159886951 1107 40-41 0.2881546607274075 1107 42-43 0.47338610852076357 1107 44-45 0.4707552911286541 1107 46-47 0.4371831697673265 1107 48-49 0.3913653625358293 1107 50-51 0.3261666724137555 1107 52-53 0.2824577439566305 1107 54-55 0.2887988018749006 1107 56-57 0.22136462532506584 1107 58-59 0.1557393832922287 1107 60-61 0.09546500963540794 1107 62-63 0.08288270253915542 1107 64-65 0.02952848565632138 1107 66-67 0.053638730941401036 1107 68-69 0.08581805742859672 1107 70-71 -0.05199032961928296 1107 72-73 -0.07458315588027986 1107 74-75 0.11156733000932562 1107 76-77 0.34166397311118146 1108 1 0.31453374600954476 1108 2 0.34079731416468917 1108 3 0.3664162179294763 1108 4 0.3238144310978228 1108 5 0.45215899390652936 1108 6 0.3182980091619214 1108 7 0.33500405495937 1108 8 0.32072987126951347 1108 9 0.2594847113058236 1108 10-11 0.3238757470249709 1108 12-13 0.31756171875566963 1108 14-15 0.34856125838757634 1108 16-17 0.3212502227759657 1108 18-19 0.2567500770137272 1108 20-21 0.2469795369939689 1108 22-23 0.267506033877865 1108 24-25 0.2526109769657481 1108 26-27 0.30570049189780235 1108 28-29 0.31623911211607947 1108 30-31 0.4390901466282102 1108 32-33 0.4309750960603935 1108 34-35 0.42152411934281986 1108 36-37 0.42206355064552525 1108 38-39 0.5252735361249918 1108 40-41 0.36602389854405004 1108 42-43 0.35565433534345203 1108 44-45 0.33513965367205145 1108 46-47 0.3412090634281135 1108 48-49 0.4179403191386335 1108 50-51 0.41910860053513943 1108 52-53 0.48323125880811446 1108 54-55 0.4485983309349493 1108 56-57 0.39892003711624824 1108 58-59 0.4644308774416466 1108 60-61 0.40888077085438823 1108 62-63 0.2948159271116175 1108 64-65 0.3727177376172328 1108 66-67 0.5030347866183966 1108 68-69 0.47600386172331 1108 70-71 0.6365799336570426 1108 72-73 0.7590040644488951 1108 74-75 0.7528824659872093 1108 76-77 0.7900894183342757 1109 1 0.10353597618922095 1109 2 -0.021186367830537733 1109 3 -0.17625717068718671 1109 4 -0.13091095839834566 1109 5 0.14542290897124133 1109 6 -0.0814534514487093 1109 7 0.1638809553237337 1109 8 0.16392978645986744 1109 9 0.20024085215266751 1109 10-11 0.020784317511214567 1109 12-13 -0.06682879041633782 1109 14-15 0.01948607620388998 1109 16-17 0.033140260783255826 1109 18-19 -0.17295803644273633 1109 20-21 -0.09661065963877036 1109 22-23 -0.1560221653305689 1109 24-25 -0.21706839544284406 1109 26-27 -0.37982061628162 1109 28-29 -0.14090040761001887 1109 30-31 -0.06769266214174507 1109 32-33 -0.19450765145813875 1109 34-35 0.06812250638892436 1109 36-37 -0.06581475536025039 1109 38-39 -0.19212068739555122 1109 40-41 -0.17170049365346784 1109 42-43 -0.16888392323175339 1109 44-45 -0.09341233935470683 1109 46-47 -0.20085220369086798 1109 48-49 -0.06501470818891875 1109 50-51 -0.23269007078035386 1109 52-53 -0.1578554826166858 1109 54-55 -0.11007129621670231 1109 56-57 -0.006069793578561189 1109 58-59 0.04737392933258633 1109 60-61 -0.03866799469404825 1109 62-63 -0.07420754474414437 1109 64-65 -0.08745372707682364 1109 66-67 6.887789541174527E-4 1109 68-69 -0.05554218677524503 1109 70-71 0.016187728292049286 1109 72-73 -0.08642622673090017 1109 74-75 -0.06024477204520551 1109 76-77 -6.453237815691182E-4 1110 1 -0.7702190302593905 1110 2 -0.4859648244676933 1110 3 -0.8082027995127348 1110 4 -0.5497259530533043 1110 5 -0.8601222240305759 1110 6 -0.593074320188073 1110 7 -0.6885055865398186 1110 8 -0.9609937164545457 1110 9 -0.919912242342555 1110 10-11 -0.7522344776415721 1110 12-13 -0.763276650337211 1110 14-15 -0.6772936891047223 1110 16-17 -0.8803586741097789 1110 18-19 -0.8641505883855132 1110 20-21 -0.9460565922232433 1110 22-23 -0.8152879828099202 1110 24-25 -0.9655537810160268 1110 26-27 -1.091769934802663 1110 28-29 -0.7871739823675981 1110 30-31 -0.5782172119933122 1110 32-33 -0.6756461733282251 1110 34-35 -0.7173980583685662 1110 36-37 -0.6038041276995685 1110 38-39 -0.7295975710631311 1110 40-41 -0.6621357105150381 1110 42-43 -0.6589844479265139 1110 44-45 -0.7850293177504817 1110 46-47 -0.9496494298424487 1110 48-49 -0.9164423193730435 1110 50-51 -0.813578005476085 1110 52-53 -0.8184731666249228 1110 54-55 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-0.5550206414721188 2314 20-21 -0.552919889424885 2314 22-23 -0.42270953484685947 2314 24-25 -0.4041920956966152 2314 26-27 -0.41965323834843105 2314 28-29 -0.5612060728211787 2314 30-31 -0.5152194231203069 2314 32-33 -0.7213345688474675 2314 34-35 -0.6864061813607023 2314 36-37 -1.3441541443983098 2314 38-39 -0.9817848260842794 2314 40-41 -0.789306255154699 2314 42-43 -0.6807109715290238 2314 44-45 -0.7088709680794025 2314 46-47 -0.5154489732705763 2314 48-49 -0.6013574213191824 2314 50-51 -0.45523988610317545 2314 52-53 -0.3986010179283781 2314 54-55 -0.4382618865533665 2314 56-57 -0.447000006066407 2314 58-59 -0.5982827388758842 2314 60-61 -0.5052311955970232 2314 62-63 -0.5745352994595017 2314 64-65 -0.5397634766159811 2314 66-67 -0.4065794242809524 2314 68-69 -0.45775079780312566 2314 70-71 -0.41518814333671017 2314 72-73 -0.3887149825138785 2314 74-75 -0.42364426722537374 2314 76-77 -0.3885204841438039 2315 1 -1.138452982599155 2315 2 -0.9248386676786637 2315 3 -0.8222378739719147 2315 4 -0.8357432668415825 2315 5 -1.0190585930164247 2315 6 -1.1024165760571876 2315 7 -1.2877879289605545 2315 8 -1.196739412847414 2315 9 -0.9855789501073033 2315 10-11 -1.1084415716747813 2315 12-13 -1.077963263274043 2315 14-15 -0.9434068543327214 2315 16-17 -0.9467913651320217 2315 18-19 -0.875698441435766 2315 20-21 -1.0695732623777374 2315 22-23 -1.0771332458463192 2315 24-25 -1.2191562012995192 2315 26-27 -1.0661081178673797 2315 28-29 -1.2132671874686096 2315 30-31 -1.302900595345065 2315 32-33 -1.2856235441877288 2315 34-35 -1.2918994370439592 2315 36-37 -1.226917217817281 2315 38-39 -1.2154855754771603 2315 40-41 -1.1867306306343792 2315 42-43 -1.278840632975598 2315 44-45 -1.27627605568847 2315 46-47 -1.0732881345379148 2315 48-49 -0.9107764524656474 2315 50-51 -0.8683905675070314 2315 52-53 -0.9672986983294152 2315 54-55 -1.070171552857179 2315 56-57 -1.1833788121196847 2315 58-59 -1.1146527043697603 2315 60-61 -1.2395217037209818 2315 62-63 -1.0947951658813047 2315 64-65 -1.002629771168241 2315 66-67 -0.9796812894691378 2315 68-69 -1.1581569923313175 2315 70-71 -1.0757366196415035 2315 72-73 -1.0147101447252673 2315 74-75 -1.237199348745765 2315 76-77 -1.274105030177516 2316 1 -1.993053918947524 2316 2 -1.4691244212701093 2316 3 -1.9485991972725074 2316 4 -2.6115708409012584 2316 5 -2.2510155292306777 2316 6 -1.9618828313267542 2316 7 -1.660618099711293 2316 8 -1.4634531590422455 2316 9 -1.8031141548844616 2316 10-11 -1.7929164682980243 2316 12-13 -2.421675642939711 2316 14-15 -3.405956152146615 2316 16-17 -2.5525631128144184 2316 18-19 -2.203484644648235 2316 20-21 -1.9913691995357325 2316 22-23 -2.5086329082358816 2316 24-25 -2.2957738484246804 2316 26-27 -2.228915280591103 2316 28-29 -2.84308069209645 2316 30-31 -3.039012522939018 2316 32-33 -3.0635983386483048 2316 34-35 -2.8670211790205755 2316 36-37 -2.8639807392467063 2316 38-39 -3.001483184614937 2316 40-41 -2.666379810468804 2316 42-43 -2.908585277403624 2316 44-45 -2.729509380356646 2316 46-47 -2.717972886378835 2316 48-49 -2.500780417189379 2316 50-51 -2.6923150365881234 2316 52-53 -2.7159556613209688 2316 54-55 -2.8194013485152887 2316 56-57 -2.527310927990648 2316 58-59 -2.720263051561986 2316 60-61 -2.494833824604612 2316 62-63 -2.5907711291610767 2316 64-65 -2.4469587052168578 2316 66-67 -2.2543747751288485 2316 68-69 -2.362051517749979 2316 70-71 -2.259351515516009 2316 72-73 -2.3443942132264333 2316 74-75 -2.6166542315578845 2316 76-77 -3.062249784541308 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 60462.0 3 5051.0 4 6567.0 5 8100.0 6 9193.0 7 9553.0 8 11122.0 9 11668.0 10 14218.0 11 16309.0 12 12763.0 13 10781.0 14 10633.0 15 11122.0 16 11116.0 17 11901.0 18 12259.0 19 12912.0 20 13919.0 21 14955.0 22 16312.0 23 18692.0 24 21601.0 25 24930.0 26 29477.0 27 34786.0 28 41029.0 29 48635.0 30 57591.0 31 69613.0 32 84666.0 33 106769.0 34 141924.0 35 189976.0 36 272144.0 37 374413.0 38 342759.0 39 92133.0 40 747.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.632708678522445 26.439806450347163 28.683230987920087 22.2442538832103 2 22.419659853359082 26.735948695744842 28.596227360753335 22.248164090142737 3 22.685206242525265 26.741697189900442 28.067551550598115 22.505545016976182 4 22.3019288803147 26.835129854811097 28.408630259959356 22.45431100491485 5 22.654655104601726 26.773395478094596 28.351317677633865 22.220631739669816 6 22.764148970373864 26.585219349898974 28.34525373988913 22.30537793983803 7 22.61838030194586 26.423763241625807 28.47753912564578 22.480317330782558 8 22.51271286779019 26.746054361975247 28.229991531444032 22.511241238790536 9 22.424111343497902 26.539111588767543 28.478436339149937 22.558340728584618 10-11 22.80077686009383 26.693795822258693 28.251574234288874 22.253853083358603 12-13 22.829157760527156 26.513724113849342 28.148782068234148 22.508336057389357 14-15 22.598888446947065 26.67047321267727 28.298273275817476 22.432365064558187 16-17 22.746705810641913 26.552876248349616 28.246377852024896 22.45404008898358 18-19 22.69552091717632 26.494748131021367 28.132269953423243 22.67746099837907 20-21 22.752667698221238 26.697204546569335 28.117673026928923 22.432454728280497 22-23 22.8831884057971 26.75736900780379 27.6813154960981 22.678127090301004 24-25 22.802732349857447 26.92237366761821 27.55054031116893 22.724353671355413 26-27 22.893172153138874 26.97533400772932 27.493148830959015 22.6383450081728 28-29 22.991861566138514 26.96132956365304 27.414926355456238 22.631882514752206 30-31 22.75437460216955 26.893996229877164 27.512042209323194 22.839586958630093 32-33 22.797372960542905 27.217353246822164 27.310080417800677 22.67519337483426 34-35 22.886120439442053 26.89446621756903 27.479174572399266 22.74023877058965 36-37 22.81784849934061 26.996596580527715 27.723722157007668 22.46183276312401 38-39 22.746736043259887 26.948708263785054 27.77485933892752 22.529696354027543 40-41 22.6731072801377 26.88016713191292 27.681022412086293 22.765703175863084 42-43 22.62995796367882 27.13792029353406 27.447690945524023 22.784430797263102 44-45 22.72434365200267 26.89111437347708 27.617342606697797 22.76719936782245 46-47 22.645183026637078 26.872491966824224 27.66558621167895 22.81673879485975 48-49 22.671122997633226 26.967550760812976 27.483871255438956 22.87745498611484 50-51 22.591487699211584 27.11902164556996 27.440712617082934 22.848778038135524 52-53 22.624623991112042 26.966535682850427 27.50671291129056 22.902127414746975 54-55 22.65493174612059 26.949004650901426 27.47084573622028 22.925217866757706 56-57 22.576294420397414 27.141060981355245 27.41288051334226 22.869764084905086 58-59 22.631869867868698 27.15123358448218 27.3778628839225 22.839033663726624 60-61 22.607263927269305 27.012700266617074 27.346434294844997 23.033601511268625 62-63 22.56839715730064 26.979531583442412 27.442707833924633 23.009363425332314 64-65 22.594709508155194 26.940542338852186 27.426373345564585 23.038374807428035 66-67 22.592092623760124 26.984046286906395 27.493776651584355 22.930084437749123 68-69 22.58632739449444 26.82327497967337 27.614664250515027 22.975733375317166 70-71 22.47648754582709 26.76917778457854 27.64414176084883 23.110192908745535 72-73 22.572340725032408 26.76814850888126 27.52700918944881 23.132501576637523 74-75 22.44787406690486 26.671316245906517 27.45723792682312 23.423571760365505 76-77 22.37337014249868 26.577495020879176 27.82388715227308 23.225247684349064 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6825.0 1 4098.5 2 1312.5 3 1269.0 4 1285.0 5 1340.5 6 1467.0 7 1613.5 8 1696.5 9 1704.0 10 1791.0 11 1869.5 12 2014.0 13 2167.0 14 2265.0 15 2482.0 16 2810.0 17 3019.0 18 3247.5 19 3892.5 20 4869.5 21 6234.0 22 7038.0 23 8234.0 24 10807.5 25 14293.5 26 16402.0 27 18501.5 28 24195.5 29 30079.5 30 32369.0 31 36960.0 32 45470.5 33 53546.5 34 61551.5 35 65400.0 36 70441.5 37 79296.5 38 84484.0 39 85858.0 40 87637.5 41 92570.0 42 98943.0 43 102163.0 44 102905.5 45 104927.0 46 106981.0 47 108869.5 48 109983.0 49 109941.0 50 109014.0 51 103788.5 52 99448.0 53 97854.5 54 91907.5 55 82706.5 56 73234.5 57 68610.0 58 64511.5 59 54298.5 60 43645.0 61 39106.0 62 34532.5 63 25285.0 64 17764.0 65 14917.0 66 12907.0 67 9522.5 68 6966.0 69 4863.0 70 3942.0 71 3315.0 72 2338.0 73 1766.0 74 1544.0 75 1345.5 76 895.5 77 515.0 78 386.0 79 355.0 80 261.0 81 176.0 82 128.0 83 102.0 84 88.5 85 70.0 86 60.0 87 55.0 88 56.0 89 42.5 90 28.5 91 29.0 92 30.0 93 31.5 94 23.5 95 17.5 96 20.0 97 22.5 98 24.0 99 50.5 100 78.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0030765101317504315 2 0.008025678604566345 3 0.011013014529599373 4 0.01836988658378519 5 0.013153195490817064 6 0.012841085767306151 7 0.015427137762110861 8 0.01729979610317634 9 0.01654181534607841 10-11 0.01966291258118754 12-13 0.019350802857676628 14-15 0.0077135688810554305 16-17 0.006242194470218267 18-19 0.011860169493414707 20-21 0.01029962087586014 22-23 0.013420718110969274 24-25 0.02149098381889432 26-27 0.013398424559289922 28-29 0.007602101122658676 30-31 0.007512926915941271 32-33 0.008939714223419734 34-35 0.01025503377250144 36-37 0.009073475533495839 38-39 0.015783834588980476 40-41 0.011637233976621197 42-43 0.01495897317684449 44-45 0.01752273161996985 46-47 0.017009979931344777 48-49 0.01330925035257252 50-51 0.02617262967155802 52-53 0.029717304388574825 54-55 0.024411439088889295 56-57 0.014290166626463962 58-59 0.01660869600111646 60-61 0.019908141649660403 62-63 0.02523630050102528 64-65 0.020398599786606124 66-67 0.01957373837447014 68-69 0.02978418504361288 70-71 0.05453002740769243 72-73 0.06601120652255818 74-75 0.07749238563742392 76-77 0.07546367243460299 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2242801.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.41182576498401 #Duplication Level Percentage of deduplicated Percentage of total 1 78.85676570214399 47.63881189988089 2 12.038183883524912 14.544973345966907 3 3.9342477261253928 7.130252644409152 4 1.705752177797057 4.121904134532714 5 0.925541964663926 2.795683995372904 6 0.5799242967560203 2.1020571343503343 7 0.38037529665089764 1.6085416302604694 8 0.2555500074442808 1.2350594019171404 9 0.2047729955737097 1.1133639476975512 >10 1.019901505570347 11.138404345454274 >50 0.06977060046344602 2.8797461408832965 >100 0.028103217068136517 2.906503485445792 >500 8.88500974445627E-4 0.35019094857122207 >1k 1.4808349574084198E-4 0.13374261543331417 >5k 7.404174787042099E-5 0.300764329823948 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6724 0.29980368298391163 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 4.4587103358701906E-5 0.0 4.4587103358701906E-5 0.0 2 0.0 4.4587103358701906E-5 0.0 2.2293551679350955E-4 0.0 3 0.0 4.4587103358701906E-5 0.0 3.121097235109134E-4 0.0 4 8.917420671740381E-5 4.4587103358701906E-5 0.0 8.025678604566343E-4 0.0 5 1.3376131007610572E-4 4.4587103358701906E-5 0.0 0.0018726583410654802 0.0 6 1.3376131007610572E-4 4.4587103358701906E-5 0.0 0.0037007295787722582 0.0 7 1.3376131007610572E-4 4.4587103358701906E-5 0.0 0.007044762330674902 4.4587103358701906E-5 8 1.3376131007610572E-4 4.4587103358701906E-5 0.0 0.01074549190944716 8.917420671740381E-5 9 1.7834841343480762E-4 4.4587103358701906E-5 0.0 0.013955763351273697 8.917420671740381E-5 10 1.7834841343480762E-4 4.4587103358701906E-5 0.0 0.017924015550198166 8.917420671740381E-5 11 3.121097235109134E-4 4.4587103358701906E-5 0.0 0.022873184023014078 8.917420671740381E-5 12 4.0128393022831717E-4 4.4587103358701906E-5 0.0 0.029070791389873646 8.917420671740381E-5 13 4.0128393022831717E-4 8.917420671740381E-5 0.0 0.03923665095565768 8.917420671740381E-5 14 4.90458136945721E-4 8.917420671740381E-5 0.0 0.05051718810540926 8.917420671740381E-5 15 4.90458136945721E-4 1.7834841343480762E-4 0.0 0.06505258380034608 8.917420671740381E-5 16 4.90458136945721E-4 1.7834841343480762E-4 0.0 0.07865165032475016 8.917420671740381E-5 17 4.90458136945721E-4 1.7834841343480762E-4 0.0 0.0934991557431979 8.917420671740381E-5 18 4.90458136945721E-4 1.7834841343480762E-4 0.0 0.10625106730378665 8.917420671740381E-5 19 6.242194470218267E-4 1.7834841343480762E-4 0.0 0.121455269549104 8.917420671740381E-5 20 7.133936537392305E-4 1.7834841343480762E-4 0.0 0.13799708489518242 8.917420671740381E-5 21 7.133936537392305E-4 1.7834841343480762E-4 0.0 0.15583192623866318 1.7834841343480762E-4 22 8.025678604566343E-4 1.7834841343480762E-4 0.0 0.17709997454076398 1.7834841343480762E-4 23 8.471549638153363E-4 1.7834841343480762E-4 0.0 0.19725334525889723 1.7834841343480762E-4 24 8.471549638153363E-4 1.7834841343480762E-4 0.0 0.2193239614214547 1.7834841343480762E-4 25 8.471549638153363E-4 1.7834841343480762E-4 0.0 0.2427321906847732 1.7834841343480762E-4 26 8.471549638153363E-4 1.7834841343480762E-4 0.0 0.2692169300798421 1.7834841343480762E-4 27 8.471549638153363E-4 1.7834841343480762E-4 0.0 0.2944086434775087 1.7834841343480762E-4 28 8.471549638153363E-4 1.7834841343480762E-4 0.0 0.3202245763221971 1.7834841343480762E-4 29 8.471549638153363E-4 1.7834841343480762E-4 0.0 0.3456392252366572 2.6752262015221145E-4 30 8.471549638153363E-4 2.2293551679350955E-4 0.0 0.37167809359813914 2.6752262015221145E-4 31 8.471549638153363E-4 2.2293551679350955E-4 0.0 0.40016925264434966 2.6752262015221145E-4 32 8.471549638153363E-4 2.2293551679350955E-4 0.0 0.4317369218223106 2.6752262015221145E-4 33 8.471549638153363E-4 2.2293551679350955E-4 0.0 0.4666486237521742 2.6752262015221145E-4 34 8.471549638153363E-4 2.2293551679350955E-4 0.0 0.5055285778809623 2.6752262015221145E-4 35 8.471549638153363E-4 2.2293551679350955E-4 0.0 0.5446760546299025 2.6752262015221145E-4 36 8.471549638153363E-4 2.2293551679350955E-4 0.0 0.5898427903322676 2.6752262015221145E-4 37 8.471549638153363E-4 2.2293551679350955E-4 0.0 0.6358120938950892 2.6752262015221145E-4 38 8.471549638153363E-4 2.2293551679350955E-4 0.0 0.6776347968455516 2.6752262015221145E-4 39 8.471549638153363E-4 2.2293551679350955E-4 0.0 0.725744281369591 2.6752262015221145E-4 40 8.471549638153363E-4 2.2293551679350955E-4 0.0 0.7721594559659997 2.6752262015221145E-4 41 8.471549638153363E-4 2.2293551679350955E-4 0.0 0.8223199472445393 2.6752262015221145E-4 42 0.001025503377250144 2.2293551679350955E-4 0.0 0.8773850198925361 2.6752262015221145E-4 43 0.001025503377250144 2.2293551679350955E-4 0.0 0.9386476999073926 2.6752262015221145E-4 44 0.0010700904806088458 2.2293551679350955E-4 0.0 1.0051270710152171 2.6752262015221145E-4 45 0.0010700904806088458 2.2293551679350955E-4 0.0 1.0748167135648683 3.121097235109134E-4 46 0.0011146775839675478 2.2293551679350955E-4 0.0 1.1477166275563457 3.121097235109134E-4 47 0.0011592646873262496 2.2293551679350955E-4 0.0 1.2222662643720954 3.121097235109134E-4 48 0.0011592646873262496 2.2293551679350955E-4 0.0 1.3012300244203565 3.121097235109134E-4 49 0.0012484388940436535 2.2293551679350955E-4 0.0 1.384028275357466 3.121097235109134E-4 50 0.0012484388940436535 2.2293551679350955E-4 0.0 1.467316984431521 3.121097235109134E-4 51 0.0012484388940436535 2.2293551679350955E-4 0.0 1.5531471583970222 3.121097235109134E-4 52 0.0012484388940436535 2.2293551679350955E-4 0.0 1.646066681796557 3.121097235109134E-4 53 0.0012484388940436535 2.2293551679350955E-4 0.0 1.7428206960849402 3.121097235109134E-4 54 0.0012484388940436535 2.2293551679350955E-4 0.0 1.8478233244946831 3.121097235109134E-4 55 0.0012484388940436535 2.2293551679350955E-4 0.0 1.9588897989612097 3.121097235109134E-4 56 0.0016051357209132687 2.2293551679350955E-4 0.0 2.0792749780297046 3.121097235109134E-4 57 0.0016943099276306725 2.2293551679350955E-4 0.0 2.2033162995736135 3.121097235109134E-4 58 0.0017834841343480764 2.2293551679350955E-4 0.0 2.3356954094455995 3.121097235109134E-4 59 0.0017834841343480764 2.2293551679350955E-4 0.0 2.4683866290410963 3.121097235109134E-4 60 0.0017834841343480764 2.2293551679350955E-4 0.0 2.5968420738175166 3.121097235109134E-4 61 0.0017834841343480764 2.2293551679350955E-4 0.0 2.7354633781597206 3.121097235109134E-4 62 0.0017834841343480764 2.2293551679350955E-4 0.0 2.876447798979936 3.121097235109134E-4 63 0.0018280712377067782 2.2293551679350955E-4 0.0 3.0270184470222725 3.121097235109134E-4 64 0.0018280712377067782 2.2293551679350955E-4 0.0 3.1869077996665776 3.121097235109134E-4 65 0.0018726583410654802 2.2293551679350955E-4 0.0 3.36097585117895 3.121097235109134E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAATCG 50 0.006735813 28.394354 36 CGCGGAA 170 1.1641532E-10 25.061106 59 ATTCGCG 175 1.6916601E-10 24.341274 56 TCGCGCT 195 6.548362E-10 21.842297 37 CTCGCGC 195 6.566552E-10 21.841808 36 GCGTAAG 190 1.153785E-8 20.550377 38 TAACGCG 145 9.883428E-6 19.587992 52 TCGCGGA 225 3.8835424E-9 18.934637 58 AATTCGC 225 3.8889993E-9 18.932947 55 CGCTCCG 245 5.493348E-10 18.834671 25 TAGGCCG 330 0.0 18.284243 3 CGCGCTT 235 6.675691E-9 18.125673 38 CGGTTTA 100 0.0089282375 17.751616 60 ACGACCG 120 0.001220811 17.748055 8 TGCGTAA 240 1.6736885E-7 16.267962 37 CAATTCG 265 2.9398507E-8 16.075144 54 CGATCGT 265 2.9454895E-8 16.072634 1 ACGAATC 275 4.6244168E-8 15.493704 48 CCTCGCG 280 5.7980287E-8 15.210921 35 TATACGA 140 0.003422684 15.210582 32 >>END_MODULE