FastQCFastQC Report
Thu 26 May 2016
SRR936130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2242801
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62420.27831269916501733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46070.20541278517353967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32180.14348129860830275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA11200.048.630558100-101
CGGTATC7950.043.427723100-101
GCGTATC2850.037.595318100-101
CGAGTAC9500.033.522495100-101
CGTACAT16500.033.190212100-101
GGTATCA72450.031.9589981
CCGTATC5000.031.548738100-101
CCGGTAT4250.030.81342100-101
TACGCAG8350.029.941185100-101
ACGTATC4600.028.46892100-101
GTATCAA130300.028.139621
AGTATCA24800.027.962782100-101
TCGTATC5400.027.558298100-101
TCGAGTA3350.027.541845100-101
AGCGTAC2950.027.23703298-99
ACCGTAT3100.026.87907898-99
CCGTACA7500.026.191408100-101
CGACGCA1600.026.042591100-101
TCCGGTA2300.025.87737398-99
CCTGTCG6150.025.64337596-97
Produced by FastQC (version 0.11.5)