FastQCFastQC Report
Thu 26 May 2016
SRR936129_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936129_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2568462
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81090.3157142289821691No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26580.103486055078876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTT2200.027.42790638
TCGCGCT2350.024.16770737
CGGTATA1201.920549E-623.66421110
CGCGGAA2153.6379788E-1223.11613559
TCGCGGA2205.456968E-1222.59032658
ATTCGCG2205.456968E-1222.58988856
CTCGCGC2550.022.27003136
CAATTCG2400.022.18952254
CGATCGT2451.8189894E-1221.7311691
TTGCGTA3400.019.83424636
CGTAAGT3500.019.2694339
CGCTCCG3150.019.15823725
CGCTCGT950.00665084318.6819134
ACGTAGG3700.018.2253
CGGTTAC2154.773392E-818.1587874
CCTCGCG3151.8189894E-1218.02952635
GCGTCGT4750.017.9321883
ACGCCCG1200.001220349817.74919557
TACGCTA1000.00894261517.7467778
TACGTAG3203.6379788E-1217.7460862
Produced by FastQC (version 0.11.5)