##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936129_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2568462 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.472894673933276 39.0 36.0 41.0 30.0 41.0 2 36.327895838054054 39.0 36.0 41.0 29.0 41.0 3 36.234430176502514 39.0 36.0 41.0 29.0 41.0 4 36.1195524792658 39.0 36.0 41.0 29.0 41.0 5 36.04542484957924 39.0 35.0 41.0 28.0 41.0 6 35.94304607192943 39.0 35.0 40.0 28.0 41.0 7 35.77792235197562 39.0 35.0 40.0 27.0 41.0 8 35.59003948666556 39.0 35.0 40.0 27.0 41.0 9 35.498145193504904 38.0 35.0 40.0 27.0 41.0 10-11 35.70902781508934 39.0 35.0 40.0 27.5 41.0 12-13 35.883370865521854 39.0 35.0 41.0 28.0 41.0 14-15 35.82697933627206 39.0 35.0 41.0 28.0 41.0 16-17 35.662169033452706 39.0 35.0 41.0 27.0 41.0 18-19 35.50225290465656 39.0 35.0 41.0 26.5 41.0 20-21 35.28225957791083 39.0 35.0 41.0 25.5 41.0 22-23 35.0076929306332 39.0 34.5 41.0 24.5 41.0 24-25 34.76718499241959 39.0 34.0 41.0 24.0 41.0 26-27 33.838034590350176 38.0 33.0 40.0 22.5 40.5 28-29 33.87356363457976 38.0 33.0 39.5 22.0 40.5 30-31 34.24721545422903 38.0 34.0 40.0 22.5 41.0 32-33 34.10076321938966 38.0 33.5 40.0 22.0 41.0 34-35 33.83512720842278 38.0 33.0 40.0 20.0 41.0 36-37 33.55146445616093 38.0 33.0 40.0 19.0 41.0 38-39 33.27457385003166 37.0 33.0 40.0 18.0 41.0 40-41 32.90535814818362 36.5 32.0 40.0 15.5 41.0 42-43 32.54961957778624 36.0 32.0 39.0 13.0 41.0 44-45 32.1842686401434 36.0 31.5 39.0 9.5 41.0 46-47 31.795994061815982 35.0 31.0 39.0 7.5 40.0 48-49 31.339009103502406 35.0 31.0 38.0 4.5 40.0 50-51 30.897423633287158 35.0 31.0 37.0 2.0 39.0 52-53 30.450534016076546 35.0 30.0 36.5 2.0 39.0 54-55 29.993328692423717 34.5 30.0 36.0 2.0 39.0 56-57 29.544801908690882 34.0 29.0 36.0 2.0 37.5 58-59 29.1136847654355 34.0 29.0 35.0 2.0 37.0 60-61 28.757471202610745 34.0 29.0 35.0 2.0 36.5 62-63 28.420047289000188 34.0 29.0 35.0 2.0 36.0 64-65 28.054302730583515 34.0 28.0 35.0 2.0 36.0 66-67 27.67587003428511 34.0 27.0 35.0 2.0 35.0 68-69 27.320659211621585 34.0 26.5 35.0 2.0 35.0 70-71 26.907265125978114 33.0 25.5 35.0 2.0 35.0 72-73 26.43503446809803 33.0 25.0 35.0 2.0 35.0 74-75 25.865046085945597 33.0 23.5 35.0 2.0 35.0 76-77 24.178058308824504 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.1407611206741493 1101 2 1.1027673262453987 1101 3 1.0683564399764833 1101 4 0.9632032925375213 1101 5 1.0120123077025696 1101 6 0.9865770207659281 1101 7 1.1818665615239823 1101 8 1.2727696644821194 1101 9 1.3606794072076625 1101 10-11 1.2665019396382604 1101 12-13 1.2725789712659434 1101 14-15 1.3618182107247279 1101 16-17 1.3239692965428844 1101 18-19 1.3026857246876418 1101 20-21 1.2762884046498755 1101 22-23 1.4708463006435366 1101 24-25 1.4099258824633125 1101 26-27 1.278793181661591 1101 28-29 1.3727568923524203 1101 30-31 1.4528025627305468 1101 32-33 1.4459746255091233 1101 34-35 1.4527423759746583 1101 36-37 1.4273106403169535 1101 38-39 1.4020985118595704 1101 40-41 1.4592472773805767 1101 42-43 1.4644646893834548 1101 44-45 1.3480188005552165 1101 46-47 1.3753943319126023 1101 48-49 1.512107670877949 1101 50-51 1.47628194830326 1101 52-53 1.447612889465752 1101 54-55 1.3300980660570012 1101 56-57 1.3517663108492357 1101 58-59 1.4544225879034585 1101 60-61 1.372851635872756 1101 62-63 1.432325733844845 1101 64-65 1.288084620744339 1101 66-67 1.4075537707439523 1101 68-69 1.5362443416789198 1101 70-71 1.5552817779773598 1101 72-73 1.4541868740582053 1101 74-75 1.3739352072111046 1101 76-77 1.2511446965057154 1102 1 1.0603399908197702 1102 2 1.0879741382784047 1102 3 0.9155882034550942 1102 4 1.0750257573196862 1102 5 1.0951251319992679 1102 6 1.025203207062134 1102 7 0.8670029802876016 1102 8 0.9223235577387854 1102 9 0.7472105456279721 1102 10-11 0.7631471278766497 1102 12-13 1.0068380184972838 1102 14-15 0.9558149582164361 1102 16-17 1.0914843929307807 1102 18-19 1.1638334106149273 1102 20-21 0.8828430357636989 1102 22-23 0.8642217003161718 1102 24-25 0.9589186438301311 1102 26-27 0.7093450267285561 1102 28-29 0.8011375715202576 1102 30-31 0.9559893423656902 1102 32-33 0.8670885777943624 1102 34-35 0.885225795391321 1102 36-37 0.9364193597531312 1102 38-39 1.0047226754094254 1102 40-41 0.9762879612978281 1102 42-43 1.0111330946790673 1102 44-45 0.9509207206800667 1102 46-47 0.7810413775155638 1102 48-49 0.8195338427562611 1102 50-51 0.9154723408941585 1102 52-53 1.0060149198451143 1102 54-55 1.1023032200316756 1102 56-57 1.0885691630488132 1102 58-59 1.1845057452536167 1102 60-61 1.0898245768696313 1102 62-63 1.2226884288045206 1102 64-65 1.1440662464048081 1102 66-67 1.2041962026193822 1102 68-69 1.273164869374007 1102 70-71 1.182001324407235 1102 72-73 1.1491348848122627 1102 74-75 1.2094635595845489 1102 76-77 1.2851550349870848 1103 1 0.682789980916823 1103 2 0.5053959049648782 1103 3 0.6601663377391134 1103 4 0.5683536440299264 1103 5 0.6222097032014915 1103 6 0.5353784892962423 1103 7 0.7068970187110537 1103 8 0.8239377387647266 1103 9 0.7633028411320311 1103 10-11 0.6922296194591411 1103 12-13 0.6845093395509636 1103 14-15 0.7951519556416642 1103 16-17 0.791585898179342 1103 18-19 0.7132429471568216 1103 20-21 0.7851070979542314 1103 22-23 0.8032566577334777 1103 24-25 0.8543806164979841 1103 26-27 0.7463573063878925 1103 28-29 0.7540242817599037 1103 30-31 1.0531744285213662 1103 32-33 1.1031501741500733 1103 34-35 1.103523973248329 1103 36-37 1.0610665671209247 1103 38-39 1.040503275528259 1103 40-41 0.9880972304600419 1103 42-43 0.9950098524528883 1103 44-45 0.9814156206602505 1103 46-47 0.8047279986286568 1103 48-49 0.8201589665431506 1103 50-51 0.832826024791963 1103 52-53 0.7693467677357866 1103 54-55 0.9563832358763982 1103 56-57 0.8037355326269484 1103 58-59 0.7648991399852463 1103 60-61 1.0065098115695719 1103 62-63 0.7258635624943572 1103 64-65 0.7511419049510515 1103 66-67 0.6213827742177216 1103 68-69 0.762896746973535 1103 70-71 0.8065309342309597 1103 72-73 0.8166618703539541 1103 74-75 0.6103040725574118 1103 76-77 0.815526148078785 1104 1 0.36138746417015 1104 2 0.5395696228614426 1104 3 0.41713688529423365 1104 4 0.30502037849687724 1104 5 0.21275020709322234 1104 6 0.09323518660863783 1104 7 0.40491250429671055 1104 8 0.5740475752304377 1104 9 0.381347209640154 1104 10-11 0.38657032946375125 1104 12-13 0.5333130034784901 1104 14-15 0.4942929259993818 1104 16-17 0.5461515695377841 1104 18-19 0.45549117078929413 1104 20-21 0.46232806587114084 1104 22-23 0.4840220551787624 1104 24-25 0.46135116405309873 1104 26-27 0.37832615893582755 1104 28-29 0.3453495739791421 1104 30-31 0.5690535948490307 1104 32-33 0.6330514569315184 1104 34-35 0.6700828374579118 1104 36-37 0.44466991509911935 1104 38-39 0.5111316824927101 1104 40-41 0.6434324350813085 1104 42-43 0.6081192075365536 1104 44-45 0.5211267575354768 1104 46-47 0.2597728485188213 1104 48-49 0.4040545337603554 1104 50-51 0.31228067317967145 1104 52-53 0.3250423308849726 1104 54-55 0.425930397756364 1104 56-57 0.293950256137375 1104 58-59 0.36597360504097765 1104 60-61 0.20426409654773892 1104 62-63 0.19925178016040235 1104 64-65 0.09750747054104991 1104 66-67 0.1918533760130714 1104 68-69 0.08985092075922907 1104 70-71 0.16611179285380118 1104 72-73 0.10788664864701047 1104 74-75 0.11148498406447871 1104 76-77 0.04985563713582053 1105 1 0.4557825112622922 1105 2 0.5168323171365827 1105 3 0.5289655668324613 1105 4 0.5311205884145167 1105 5 0.5797978666720027 1105 6 0.27139682825576017 1105 7 0.32531318859781067 1105 8 0.6725455579607811 1105 9 0.4654922639096881 1105 10-11 0.4969202991498207 1105 12-13 0.4503389619982343 1105 14-15 0.15864222354370128 1105 16-17 0.3626553516017381 1105 18-19 0.5234483327298491 1105 20-21 0.47534312826379477 1105 22-23 0.3444729305673988 1105 24-25 0.3738717462832284 1105 26-27 0.2084269633104867 1105 28-29 0.3117450196277005 1105 30-31 0.23336936974571643 1105 32-33 0.3814307171365101 1105 34-35 0.512975360640894 1105 36-37 0.48845066178443375 1105 38-39 0.5021442353309808 1105 40-41 0.5308352658451341 1105 42-43 0.4647489232948985 1105 44-45 0.32006315232249705 1105 46-47 0.38700717098135584 1105 48-49 0.4412662769886957 1105 50-51 0.5355189609407773 1105 52-53 0.38776742659761965 1105 54-55 0.25938456461302195 1105 56-57 0.3505271125067644 1105 58-59 0.27800021374808637 1105 60-61 0.17063497018002494 1105 62-63 0.19564602676211607 1105 64-65 0.26716305700161413 1105 66-67 0.293886604809785 1105 68-69 0.06563397434311113 1105 70-71 -0.3340428166369058 1105 72-73 -0.3232677125903365 1105 74-75 -0.18017226755128135 1105 76-77 -0.08670921187716019 1106 1 0.13482638941222547 1106 2 0.19600453023809195 1106 3 -0.0635540931394587 1106 4 0.10904347068522213 1106 5 0.012309641321124332 1106 6 0.15356268563494524 1106 7 0.1381955370348109 1106 8 0.1580558638693006 1106 9 -0.04690834885662554 1106 10-11 -0.14730602549097682 1106 12-13 -0.18921570579698965 1106 14-15 -0.14289298331454603 1106 16-17 0.05801008941888597 1106 18-19 -0.023798444301569077 1106 20-21 -0.17388296732556796 1106 22-23 -0.2402898242481868 1106 24-25 -0.11649544217416974 1106 26-27 -0.20598966585623657 1106 28-29 -0.21973714818116008 1106 30-31 -0.2632458785964715 1106 32-33 -0.20029202082353947 1106 34-35 -0.2507905888219284 1106 36-37 -0.2118091668784885 1106 38-39 -0.26818904028469603 1106 40-41 -0.39986179305184066 1106 42-43 -0.2238968403203927 1106 44-45 -0.37974105418630444 1106 46-47 -0.0660300922956445 1106 48-49 -0.3615969320102259 1106 50-51 -0.4164983206173396 1106 52-53 -0.3467318694620154 1106 54-55 -0.22993824856188283 1106 56-57 -0.42121503611470246 1106 58-59 -0.45960483775375494 1106 60-61 -0.6350847664621035 1106 62-63 -0.609170899956478 1106 64-65 -0.6359080016972349 1106 66-67 -0.5275654919369082 1106 68-69 -0.6959397793070323 1106 70-71 -0.8216493979046007 1106 72-73 -0.7648312358279732 1106 74-75 -0.8749821606545503 1106 76-77 -0.7996393055467941 1107 1 -6.950098858879983E-4 1107 2 0.23352445179278902 1107 3 0.2086898788921019 1107 4 0.13280464399078795 1107 5 0.19653947503037728 1107 6 -0.07186780243402069 1107 7 0.059062295999616765 1107 8 -0.03959402124466038 1107 9 -0.09307655693458372 1107 10-11 0.1325323473367206 1107 12-13 0.1510062476954559 1107 14-15 0.04229981678213335 1107 16-17 0.07950809300537287 1107 18-19 0.021934632413753263 1107 20-21 -0.0627586900775512 1107 22-23 -0.16479400564564628 1107 24-25 -0.1814271796743796 1107 26-27 -0.171034870043556 1107 28-29 -5.229962331227966E-4 1107 30-31 0.09343263759341625 1107 32-33 0.21878165628385204 1107 34-35 0.22813064226323831 1107 36-37 0.27205415077714434 1107 38-39 0.26362834891090614 1107 40-41 0.191974769499609 1107 42-43 0.2170097252562968 1107 44-45 0.2363833812175713 1107 46-47 0.035671259166406344 1107 48-49 0.1292278386029544 1107 50-51 -0.006586571583916623 1107 52-53 0.18727512670412239 1107 54-55 0.14294491643684637 1107 56-57 0.13492266815980614 1107 58-59 0.1526699219507286 1107 60-61 0.13989035440546616 1107 62-63 0.17414763161102798 1107 64-65 0.1793408991195804 1107 66-67 0.2686876742216313 1107 68-69 0.1076315420891838 1107 70-71 0.13961085282514674 1107 72-73 0.13870675118053555 1107 74-75 0.06428850565200861 1107 76-77 0.07526622674498284 1108 1 0.11115491663469612 1108 2 0.18876091763661407 1108 3 0.2893295414821466 1108 4 0.23318175476139658 1108 5 0.13086188202173332 1108 6 0.2366564363725061 1108 7 -0.014677508535740458 1108 8 0.3101553027133832 1108 9 0.2435898277215358 1108 10-11 0.1676787734797216 1108 12-13 0.19372520538447446 1108 14-15 0.3488785744943357 1108 16-17 0.32938306832945585 1108 18-19 0.2970979635223401 1108 20-21 0.2825121497185279 1108 22-23 0.19202947455251262 1108 24-25 0.14543549998984417 1108 26-27 0.10299932216855723 1108 28-29 0.2096097210638348 1108 30-31 0.3603825681874824 1108 32-33 0.45120221547943373 1108 34-35 0.4146484230282397 1108 36-37 0.2713304803785306 1108 38-39 0.2262997094864616 1108 40-41 0.3925859490243866 1108 42-43 0.361392623510028 1108 44-45 0.3391122249429941 1108 46-47 0.24825637151627689 1108 48-49 0.42316202674158987 1108 50-51 0.31296968482007514 1108 52-53 0.3316595133468496 1108 54-55 0.4144735232734078 1108 56-57 0.35818744159566407 1108 58-59 0.342473438935599 1108 60-61 0.32185742497390635 1108 62-63 0.3589549400710297 1108 64-65 0.34107723805865575 1108 66-67 0.3393616324276678 1108 68-69 0.33057670357155544 1108 70-71 0.1777819987593361 1108 72-73 0.1633303454362931 1108 74-75 0.009548065026194763 1108 76-77 0.050319103722582526 1109 1 -0.08146431979897528 1109 2 -0.1472454912711214 1109 3 -0.3032516622919843 1109 4 -0.11098549183589768 1109 5 -0.23505680900741055 1109 6 -0.20365656432340984 1109 7 -0.3420416024683206 1109 8 -0.1517351632271513 1109 9 -0.13137173501616672 1109 10-11 -0.2930444134953092 1109 12-13 -0.26455620392786017 1109 14-15 -0.1985226619717011 1109 16-17 -0.060132055721751954 1109 18-19 -0.2507220368013421 1109 20-21 -0.2598821275800205 1109 22-23 -0.15870649786976543 1109 24-25 -0.26902945331899986 1109 26-27 -0.2833113480154523 1109 28-29 -0.18285698012893192 1109 30-31 -0.11705273583103093 1109 32-33 -0.22580340040688895 1109 34-35 -0.1632558155902899 1109 36-37 -0.20525111181424904 1109 38-39 -0.2111648877719503 1109 40-41 -0.21901158451718317 1109 42-43 -0.013117807149150451 1109 44-45 -0.08280955725124528 1109 46-47 -0.18873686352556263 1109 48-49 0.1328292005103826 1109 50-51 0.15165254099188274 1109 52-53 0.1569803633363307 1109 54-55 0.19301622504880456 1109 56-57 0.08186803199201265 1109 58-59 -0.01715165614605141 1109 60-61 0.0332482065045987 1109 62-63 0.15257111770782572 1109 64-65 0.033939236148931684 1109 66-67 0.17928661907577848 1109 68-69 0.1561726834385233 1109 70-71 0.057463252704213374 1109 72-73 0.18142013488101583 1109 74-75 0.12134789994826889 1109 76-77 0.22258277524987946 1110 1 -0.33746204788366185 1110 2 -0.3344507757724031 1110 3 -0.48403562230723196 1110 4 -0.3607082924998011 1110 5 -0.3183677823404665 1110 6 -0.4608166642136595 1110 7 -0.41869397166829003 1110 8 -0.44636933024277425 1110 9 -0.4113972358627578 1110 10-11 -0.4577463350426001 1110 12-13 -0.427794975601266 1110 14-15 -0.4098211576784294 1110 16-17 -0.5816801815557753 1110 18-19 -0.5263130198165911 1110 20-21 -0.6743637073670143 1110 22-23 -0.6591239757393268 1110 24-25 -0.48374305976966525 1110 26-27 -0.47729611496058055 1110 28-29 -0.45787513426042636 1110 30-31 -0.4875588607798065 1110 32-33 -0.47385076799066894 1110 34-35 -0.2878903079327202 1110 36-37 -0.4008876584910368 1110 38-39 -0.31494630456723627 1110 40-41 -0.3074308603397 1110 42-43 -0.2918793088696532 1110 44-45 -0.23673308813953753 1110 46-47 -0.42339374969430565 1110 48-49 -0.3827148548584489 1110 50-51 -0.358807909863323 1110 52-53 -0.5759654978052318 1110 54-55 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2314 14-15 -0.8646967060452297 2314 16-17 -1.4058250106286252 2314 18-19 -1.1716193310878111 2314 20-21 -0.9494381090174429 2314 22-23 -0.6908979485534843 2314 24-25 -0.6476229627114805 2314 26-27 -0.7356104807269546 2314 28-29 -0.8542857089030278 2314 30-31 -0.8425616436852934 2314 32-33 -0.9545354000796067 2314 34-35 -0.9459430357775034 2314 36-37 -1.0147290288952604 2314 38-39 -0.9356482962115464 2314 40-41 -0.7400870988272317 2314 42-43 -0.7453904743445037 2314 44-45 -0.7959828387234964 2314 46-47 -0.7183423406181504 2314 48-49 -0.5616590384366198 2314 50-51 -0.5586889145170986 2314 52-53 -0.5473160084858151 2314 54-55 -0.505636294157835 2314 56-57 -0.46498723425758115 2314 58-59 -0.4791095746117051 2314 60-61 -0.5201591893641364 2314 62-63 -0.5188940627779708 2314 64-65 -0.3936021037635449 2314 66-67 -0.588477558804378 2314 68-69 -0.5203957366865986 2314 70-71 -0.3275435288876203 2314 72-73 -0.28375051682314023 2314 74-75 -0.34863227976128996 2314 76-77 -0.4958845273024757 2315 1 -1.1878954676335098 2315 2 -1.0292643702969855 2315 3 -1.2730784766482302 2315 4 -1.0457402947317007 2315 5 -0.9958329467373233 2315 6 -0.9408083383890471 2315 7 -1.1194072365017291 2315 8 -1.0355958984347424 2315 9 -1.0125464299587321 2315 10-11 -1.0301450248892365 2315 12-13 -1.0909164914620746 2315 14-15 -1.3505845176417992 2315 16-17 -1.2555350896681858 2315 18-19 -1.1307647576322282 2315 20-21 -1.1557660906405758 2315 22-23 -1.1118478859130434 2315 24-25 -1.1723959571178284 2315 26-27 -1.1205245172871514 2315 28-29 -1.1235466529436522 2315 30-31 -1.2434820929720516 2315 32-33 -1.3430298719732363 2315 34-35 -1.2031371181919042 2315 36-37 -1.4559034358786107 2315 38-39 -1.2853187850884709 2315 40-41 -1.2967340781857999 2315 42-43 -1.3519681819157228 2315 44-45 -1.3260944565886916 2315 46-47 -1.3478271912224713 2315 48-49 -1.2576443100163353 2315 50-51 -1.1569479041233564 2315 52-53 -1.1283218801468244 2315 54-55 -1.2471746117166909 2315 56-57 -1.3838082106213783 2315 58-59 -1.206063426118682 2315 60-61 -1.3664409802647342 2315 62-63 -1.575716723483243 2315 64-65 -1.526637367161296 2315 66-67 -1.3735132581338085 2315 68-69 -1.4742025947585056 2315 70-71 -1.440867565131093 2315 72-73 -2.876668683517078 2315 74-75 -3.3550916897271037 2315 76-77 -2.973351071415596 2316 1 -3.2041130232567383 2316 2 -3.1311232107135183 2316 3 -2.7559540260690625 2316 4 -2.7598833234478306 2316 5 -3.1878147081694834 2316 6 -2.860848575826992 2316 7 -3.450553863248153 2316 8 -3.0758230691729054 2316 9 -2.621816065868522 2316 10-11 -2.6440111770602712 2316 12-13 -2.7877411821436553 2316 14-15 -2.813265783944125 2316 16-17 -4.068647929287387 2316 18-19 -3.877962561150923 2316 20-21 -3.2608992312842915 2316 22-23 -3.372265282970517 2316 24-25 -3.442952096707778 2316 26-27 -3.1612535291109687 2316 28-29 -3.2252039054989297 2316 30-31 -3.490449823388232 2316 32-33 -3.6121509814527215 2316 34-35 -3.612586793347468 2316 36-37 -3.5169661519908146 2316 38-39 -3.580945755891534 2316 40-41 -3.5182184735324 2316 42-43 -3.817554128962975 2316 44-45 -3.609663155227608 2316 46-47 -3.2474070120020997 2316 48-49 -3.2782296861066627 2316 50-51 -3.677865023715796 2316 52-53 -4.572600678913556 2316 54-55 -3.8322443135369433 2316 56-57 -3.8299819352839144 2316 58-59 -4.0207065179405745 2316 60-61 -3.637664926598788 2316 62-63 -3.5509877151652987 2316 64-65 -3.3853329159009817 2316 66-67 -3.6544029667855114 2316 68-69 -3.6198769950533993 2316 70-71 -3.4136229656824177 2316 72-73 -3.3977103243070417 2316 74-75 -3.3920041800609013 2316 76-77 -3.3916481372980165 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 69916.0 3 5754.0 4 7630.0 5 8922.0 6 10062.0 7 10615.0 8 12665.0 9 13629.0 10 16352.0 11 18710.0 12 14747.0 13 12379.0 14 11911.0 15 12487.0 16 12439.0 17 13353.0 18 14165.0 19 14829.0 20 15704.0 21 16946.0 22 18687.0 23 21379.0 24 24573.0 25 29030.0 26 33897.0 27 40790.0 28 47455.0 29 55743.0 30 66799.0 31 80625.0 32 97297.0 33 123818.0 34 163603.0 35 218105.0 36 307386.0 37 423257.0 38 393393.0 39 108537.0 40 873.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.358342934639346 26.84583566795303 28.945611116151337 21.85021028125629 2 22.03318618271675 27.010490565655694 29.007401142549188 21.948922109078364 3 22.253161353127282 27.28438062789 28.461893404721344 22.00056461426138 4 21.98814533067612 26.961779555484945 28.88779339761427 22.162281716224665 5 22.17269275218755 27.160100118060814 28.800530183383643 21.866676946367996 6 22.22199725023664 27.070294962933485 28.799663268854196 21.90804451797567 7 22.159585619740227 26.760228279848413 28.998285097481542 22.08190100292982 8 22.161202970979446 27.10921394587783 28.663666715731285 22.065916367411436 9 22.095303959374736 26.93386478571153 28.760265056422035 22.210566198491698 10-11 22.444316587664805 27.058667764807133 28.60322958437136 21.893786063156707 12-13 22.42108523847564 26.846156736679642 28.55475402456668 22.17800400027803 14-15 22.153918037574226 27.1600311496155 28.639968850384502 22.046081962425777 16-17 22.385031972498552 26.942169161636425 28.706740370384935 21.966058495480087 18-19 22.25262631169557 26.899856990014616 28.499037676170484 22.34847902211933 20-21 22.247342626433348 27.12298786341129 28.432874909214114 22.196794600941253 22-23 22.458894620888124 27.142858533631497 28.009875610532557 22.388371234947822 24-25 22.39530133782976 27.369590317625047 27.91334772864895 22.321760615896245 26-27 22.47025261207856 27.264591646528068 27.817723950237372 22.447431791155996 28-29 22.59489990345549 27.3526421052816 27.737165313830005 22.3152926774329 30-31 22.387028734375672 27.25382320628931 27.89626943531743 22.462878624017588 32-33 22.331699960692365 27.622674760781223 27.70222453166192 22.343400746864493 34-35 22.39051457274692 27.30383739267566 27.918248544672235 22.387399489905185 36-37 22.48525848059602 27.447641249815284 27.969569010480804 22.097531259107893 38-39 22.39288725795313 27.323569135207087 28.074934869759964 22.208608737079818 40-41 22.23429950280023 27.18540695892777 28.080339290463336 22.499954247808667 42-43 22.229149135756128 27.482795532698905 27.73973816266842 22.54831716887655 44-45 22.28639805491804 27.278575205656434 27.995267351422328 22.4397593880032 46-47 22.259878072929983 27.264850138043688 28.027298360708087 22.447973428318242 48-49 22.361897351631036 27.300010300391747 27.839447073413364 22.498645274563856 50-51 22.170455983875524 27.45558465837723 27.795289237689992 22.578670120057254 52-53 22.197275402182274 27.416302055551757 27.883222396908387 22.50320014535758 54-55 22.201719957409985 27.331390507910076 27.85860551193307 22.60828402274687 56-57 22.169449639924967 27.597912254077883 27.625599849217487 22.607038256779663 58-59 22.34213662544774 27.54399228515338 27.649500951213486 22.464370138185394 60-61 22.29294464342562 27.403670448228496 27.5899869253303 22.713397983015586 62-63 22.162858835801302 27.369318605553662 27.76839555876712 22.69942699987791 64-65 22.251925683924963 27.233857610574702 27.817219405488537 22.6969973000118 66-67 22.18003884323945 27.353269799283968 27.92006798151071 22.546623375965872 68-69 22.287617734805995 27.20724461360159 27.90731914514058 22.597818506451837 70-71 22.08175459830417 27.096361772541005 27.958495719130887 22.863387910023935 72-73 22.255125697488623 26.97500896732814 27.922593984515604 22.847271350667636 74-75 22.063775290513824 26.966981512561816 27.838669113317632 23.130574083606724 76-77 21.963415967183135 26.824432767063268 28.20199916644568 23.010152099307923 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8204.0 1 4963.5 2 1608.5 3 1531.0 4 1568.0 5 1639.5 6 1806.5 7 1963.0 8 2118.0 9 2212.0 10 2266.5 11 2378.0 12 2556.5 13 2678.0 14 2801.0 15 3114.0 16 3582.5 17 3861.0 18 4047.0 19 4783.5 20 6001.0 21 7955.5 22 9243.0 23 10495.5 24 13496.5 25 17667.5 26 20090.0 27 22778.5 28 29600.5 29 37324.5 30 40915.0 31 47253.0 32 59606.0 33 70844.5 34 80118.0 35 84168.0 36 91206.5 37 101926.0 38 107037.0 39 108467.0 40 109311.0 41 112044.5 42 115881.5 43 117829.0 44 117479.0 45 116611.5 46 117542.5 47 119236.5 48 119482.0 49 119033.0 50 116769.5 51 111122.0 52 107289.0 53 104375.0 54 96410.0 55 86333.0 56 75791.5 57 70276.0 58 66187.0 59 56173.5 60 45393.0 61 40537.0 62 36168.5 63 26961.0 64 19222.5 65 16323.0 66 14069.5 67 10578.5 68 7909.0 69 5525.5 70 4574.0 71 3875.0 72 2700.5 73 1952.5 74 1680.0 75 1564.5 76 1137.5 77 674.0 78 522.0 79 439.5 80 300.0 81 206.0 82 146.0 83 123.0 84 108.5 85 84.0 86 69.5 87 65.0 88 59.0 89 48.5 90 40.5 91 37.0 92 37.5 93 37.0 94 35.0 95 32.0 96 30.0 97 28.5 98 34.5 99 86.5 100 131.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009149444297793777 2 0.013432162905271716 3 0.013120692461091502 4 0.014016169988109617 5 0.011446538823622853 6 0.009383047130928936 7 0.01502844893169531 8 0.016352198319461216 9 0.015768191236623316 10-11 0.01886342877566419 12-13 0.01520365105654668 14-15 0.008253966770775664 16-17 0.0062683426891268 18-19 0.017890083637601025 20-21 0.023632819952173714 22-23 0.020226111968952628 24-25 0.019350101344695773 26-27 0.024586698187475615 28-29 0.008429168895627033 30-31 0.009013175978464934 32-33 0.010064388727573155 34-35 0.012147347323028334 36-37 0.011310270504294008 38-39 0.013179093169375293 40-41 0.010862531740784952 42-43 0.014755912293037623 44-45 0.013393229099749188 46-47 0.02396375729911519 48-49 0.023301882605232235 50-51 0.03648097577460752 52-53 0.038953272425287976 54-55 0.02818807519830934 56-57 0.020440247899326525 58-59 0.01660526805535764 60-61 0.020226111968952628 62-63 0.025754712353151417 64-65 0.026202451116660478 66-67 0.02744833289338133 68-69 0.04253518253336043 70-71 0.05456572843982119 72-73 0.08571277285784255 74-75 0.08693918773180215 76-77 0.09120243943651882 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2568462.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.72436899327985 #Duplication Level Percentage of deduplicated Percentage of total 1 77.73272348720278 44.870724134278845 2 12.483854714410947 14.412452719863076 3 4.247834193017587 7.35610645140055 4 1.9194705112051311 4.43200896242098 5 1.0276779386459334 2.9661030268325534 6 0.6079512847896669 2.1056162575882373 7 0.3876219457882265 1.5662662560000893 8 0.26228081297421907 1.2111995542384992 9 0.19006790814993307 0.9874395057444801 >10 1.0012969005017625 10.605609634175886 >50 0.08957711935340913 3.5619647213601393 >100 0.04794923736709278 4.934368262243211 >500 0.001558430865861544 0.5690130039725558 >1k 6.775786373281481E-5 0.10396182049446322 >5k 6.775786373281481E-5 0.3171656893866073 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8109 0.3157142289821691 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2658 0.103486055078876 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.504042497027404E-4 0.0 3 0.0 0.0 0.0 5.061394717928473E-4 0.0 4 0.0 0.0 0.0 7.397423049280075E-4 0.0 5 0.0 0.0 0.0 0.001479484609856015 0.0 6 0.0 0.0 0.0 0.002920035414189503 0.0 7 0.0 0.0 0.0 0.006346210300171854 0.0 8 0.0 0.0 0.0 0.010239590852424525 0.0 9 0.0 0.0 0.0 0.013548964321839295 0.0 10 0.0 0.0 0.0 0.0176370139017046 0.0 11 0.0 0.0 0.0 0.022659474814110545 0.0 12 0.0 0.0 0.0 0.02994009644682304 0.0 13 0.0 0.0 0.0 0.03928420977222945 0.0 14 0.0 3.893380552252671E-5 0.0 0.05135368948421273 0.0 15 0.0 3.893380552252671E-5 0.0 0.06610960177725035 0.0 16 0.0 3.893380552252671E-5 0.0 0.08164419018073851 0.0 17 0.0 3.893380552252671E-5 0.0 0.09585502919646076 0.0 18 0.0 3.893380552252671E-5 0.0 0.110065868212183 0.0 19 0.0 3.893380552252671E-5 0.0 0.1231865606732745 3.893380552252671E-5 20 0.0 3.893380552252671E-5 0.0 0.1396166266037808 3.893380552252671E-5 21 0.0 3.893380552252671E-5 0.0 0.1581491180325035 3.893380552252671E-5 22 0.0 3.893380552252671E-5 0.0 0.17835576309869486 3.893380552252671E-5 23 0.0 3.893380552252671E-5 0.0 0.19891281241458897 3.893380552252671E-5 24 0.0 3.893380552252671E-5 0.0 0.2235968451158709 3.893380552252671E-5 25 0.0 3.893380552252671E-5 0.0 0.248514480650288 3.893380552252671E-5 26 0.0 3.893380552252671E-5 0.0 0.2750284022111287 3.893380552252671E-5 27 0.0 3.893380552252671E-5 0.0 0.3032554112149605 3.893380552252671E-5 28 0.0 3.893380552252671E-5 0.0 0.3297693327758012 3.893380552252671E-5 29 0.0 3.893380552252671E-5 0.0 0.3573734008912727 3.893380552252671E-5 30 0.0 3.893380552252671E-5 0.0 0.3862622845889875 3.893380552252671E-5 31 0.0 3.893380552252671E-5 0.0 0.4180712037008918 3.893380552252671E-5 32 0.0 3.893380552252671E-5 0.0 0.45163214406130986 3.893380552252671E-5 33 0.0 3.893380552252671E-5 0.0 0.4882688550580075 3.893380552252671E-5 34 0.0 3.893380552252671E-5 0.0 0.5304731002444264 3.893380552252671E-5 35 0.0 3.893380552252671E-5 0.0 0.5748965723456294 3.893380552252671E-5 36 0.0 3.893380552252671E-5 0.0 0.6210331318898236 3.893380552252671E-5 37 0.0 3.893380552252671E-5 0.0 0.6691553155156665 3.893380552252671E-5 38 0.0 3.893380552252671E-5 0.0 0.7147857355880679 3.893380552252671E-5 39 0.0 3.893380552252671E-5 0.0 0.7604550894659917 3.893380552252671E-5 40 0.0 3.893380552252671E-5 0.0 0.8104850295624385 3.893380552252671E-5 41 0.0 3.893380552252671E-5 0.0 0.8628120641847145 3.893380552252671E-5 42 0.0 3.893380552252671E-5 0.0 0.9202783611359638 3.893380552252671E-5 43 0.0 3.893380552252671E-5 0.0 0.986816234773962 3.893380552252671E-5 44 0.0 3.893380552252671E-5 0.0 1.0544442549665909 7.786761104505341E-5 45 0.0 3.893380552252671E-5 0.0 1.1235517597690758 7.786761104505341E-5 46 0.0 3.893380552252671E-5 0.0 1.2076487796977335 7.786761104505341E-5 47 0.0 3.893380552252671E-5 0.0 1.2910839249325083 7.786761104505341E-5 48 0.0 3.893380552252671E-5 0.0 1.3750641434445983 7.786761104505341E-5 49 0.0 3.893380552252671E-5 0.0 1.4574480759302648 7.786761104505341E-5 50 0.0 3.893380552252671E-5 0.0 1.5401434788601116 7.786761104505341E-5 51 0.0 3.893380552252671E-5 0.0 1.6315600542270043 7.786761104505341E-5 52 0.0 3.893380552252671E-5 0.0 1.7240278423430053 7.786761104505341E-5 53 0.0 3.893380552252671E-5 0.0 1.828681911587557 7.786761104505341E-5 54 0.0 3.893380552252671E-5 0.0 1.9426022265464702 7.786761104505341E-5 55 0.0 3.893380552252671E-5 0.0 2.061077796751519 7.786761104505341E-5 56 0.0 3.893380552252671E-5 0.0 2.1911556410022808 7.786761104505341E-5 57 0.0 3.893380552252671E-5 0.0 2.3274239603311244 7.786761104505341E-5 58 0.0 3.893380552252671E-5 0.0 2.464003750104148 7.786761104505341E-5 59 0.0 3.893380552252671E-5 0.0 2.598831518628658 7.786761104505341E-5 60 0.0 3.893380552252671E-5 0.0 2.739810828425727 7.786761104505341E-5 61 0.0 3.893380552252671E-5 0.0 2.8877982232168513 7.786761104505341E-5 62 0.0 3.893380552252671E-5 0.0 3.0363306912852908 7.786761104505341E-5 63 0.0 3.893380552252671E-5 0.0 3.196465433399443 7.786761104505341E-5 64 0.0 3.893380552252671E-5 0.0 3.370927815945885 7.786761104505341E-5 65 0.0 3.893380552252671E-5 0.0 3.555357252706094 7.786761104505341E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGCTT 220 0.0 27.427906 38 TCGCGCT 235 0.0 24.167707 37 CGGTATA 120 1.920549E-6 23.664211 10 CGCGGAA 215 3.6379788E-12 23.116135 59 TCGCGGA 220 5.456968E-12 22.590326 58 ATTCGCG 220 5.456968E-12 22.589888 56 CTCGCGC 255 0.0 22.270031 36 CAATTCG 240 0.0 22.189522 54 CGATCGT 245 1.8189894E-12 21.731169 1 TTGCGTA 340 0.0 19.834246 36 CGTAAGT 350 0.0 19.26943 39 CGCTCCG 315 0.0 19.158237 25 CGCTCGT 95 0.006650843 18.68191 34 ACGTAGG 370 0.0 18.225 3 CGGTTAC 215 4.773392E-8 18.158787 4 CCTCGCG 315 1.8189894E-12 18.029526 35 GCGTCGT 475 0.0 17.932188 3 ACGCCCG 120 0.0012203498 17.749195 57 TACGCTA 100 0.008942615 17.746777 8 TACGTAG 320 3.6379788E-12 17.746086 2 >>END_MODULE