FastQCFastQC Report
Thu 26 May 2016
SRR936129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936129_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2568462
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87910.3422670843485323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62670.2439981592096749No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44730.17415091210226197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12100.048.705452100-101
CGGTATC9900.038.483322100-101
GCGTATC3900.037.396355100-101
CGAGTAC12650.037.17614100-101
CGTACAT19000.035.560677100-101
GGTATCA82950.035.008261
TCGAGTA6000.032.74089100-101
TCGGTAT4100.032.66829100-101
TACGCAG10700.032.267998100-101
TCGTATC5100.031.515295100-101
AGGTATC21200.030.88763100-101
GTATCAA160800.030.6415961
ACGTATC4050.029.396982100-101
CCGGTAT4600.028.470337100-101
GGGTATC14050.028.175594100-101
CCGTACA7750.028.036186100-101
TCGTACA10150.027.858347100-101
CCGTATC5600.027.63841100-101
AGCGTAC3800.027.41355198-99
GCGTACA6750.027.339193100-101
Produced by FastQC (version 0.11.5)