FastQCFastQC Report
Thu 26 May 2016
SRR936128_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936128_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581389
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81990.3176197000916948No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26210.10153448395418127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT2500.026.972221
CGCGGAA2700.024.98444259
CGGTATA1001.2552544E-524.84668410
CAATTCG2800.024.08887354
TCGCGCT2850.023.66350737
CGGTTAC2350.022.6543564
ATTCGCG3000.022.48338356
AACTCGA3350.022.24946813
CGCGCTT3400.020.88118638
TAACGCG1557.629824E-720.61654552
CTCGCGC3350.020.13008136
TCGCGGA3450.019.5534258
AATTCGC3350.019.07429955
CGCTCCG3400.018.79306625
TAGCGCG950.006654223418.6802715
ACGATCG1752.4415476E-618.25930659
CGCGCTG1200.00122107417.74763126
ACTCGAG4200.017.74694414
AACGCGA1854.1503845E-617.27164653
TACCGCG1300.002088698316.3798935
Produced by FastQC (version 0.11.5)