##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936128_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2581389 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.45729721479405 39.0 36.0 41.0 30.0 41.0 2 36.32247948681892 39.0 36.0 41.0 29.0 41.0 3 36.18702605457759 39.0 36.0 41.0 29.0 41.0 4 36.078519355277336 39.0 35.0 41.0 28.0 41.0 5 36.00986561885868 39.0 35.0 41.0 28.0 41.0 6 35.91006702205673 39.0 35.0 40.0 28.0 41.0 7 35.76290051596253 39.0 35.0 40.0 27.0 41.0 8 35.62892884412229 39.0 35.0 40.0 27.0 41.0 9 35.79353789762023 39.0 35.0 40.0 28.0 41.0 10-11 36.01003277692746 39.0 35.0 41.0 29.0 41.0 12-13 35.924867387286454 39.0 35.0 41.0 28.0 41.0 14-15 35.77479837405366 39.0 35.0 41.0 27.0 41.0 16-17 35.625272091885414 39.0 35.0 41.0 27.0 41.0 18-19 35.46407515488754 39.0 35.0 41.0 26.5 41.0 20-21 35.24323261623878 39.0 35.0 41.0 25.5 41.0 22-23 34.95330788966716 39.0 34.5 41.0 24.0 41.0 24-25 34.714458766191385 39.0 34.0 41.0 24.0 41.0 26-27 33.765371666184365 38.0 33.0 40.0 22.0 40.5 28-29 33.779489453158746 38.0 33.0 39.5 22.0 40.5 30-31 34.1920154227046 38.0 34.0 40.0 22.0 41.0 32-33 34.079480659443426 38.0 33.5 40.0 21.5 41.0 34-35 33.83061948431639 38.0 33.0 40.0 20.0 41.0 36-37 33.53158202812517 38.0 33.0 40.0 18.5 41.0 38-39 33.22901120288341 37.0 33.0 40.0 18.0 41.0 40-41 32.878760814429754 36.5 32.0 40.0 15.0 41.0 42-43 32.51583643534546 36.0 32.0 39.0 12.5 41.0 44-45 32.15421135675406 36.0 31.5 39.0 8.0 41.0 46-47 31.742895975771184 35.0 31.0 39.0 7.5 40.0 48-49 31.32720698042798 35.0 31.0 38.0 4.5 40.0 50-51 30.87002307672342 35.0 30.5 37.0 2.0 39.0 52-53 30.386677095160785 35.0 30.0 36.5 2.0 39.0 54-55 29.92869517147551 34.5 30.0 36.0 2.0 38.5 56-57 29.49025408413842 34.0 29.0 36.0 2.0 37.0 58-59 29.06483292521972 34.0 29.0 35.0 2.0 37.0 60-61 28.697724170979267 34.0 29.0 35.0 2.0 36.5 62-63 28.332831278044495 34.0 29.0 35.0 2.0 36.0 64-65 27.96085518300419 34.0 27.5 35.0 2.0 36.0 66-67 27.611027628923807 34.0 27.0 35.0 2.0 35.0 68-69 27.251304820776724 33.5 26.0 35.0 2.0 35.0 70-71 26.809813243955098 33.0 25.5 35.0 2.0 35.0 72-73 26.34288264961228 33.0 25.0 35.0 2.0 35.0 74-75 25.788721885775452 33.0 23.5 35.0 2.0 35.0 76-77 24.03984579619732 31.0 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9617933532578391 1101 2 0.8420548575246869 1101 3 1.0264881324823065 1101 4 0.9870648505253143 1101 5 0.8858723757435314 1101 6 1.0550701481754174 1101 7 1.034850630054983 1101 8 0.9086638623781766 1101 9 0.8815075694085408 1101 10-11 0.9977027401081529 1101 12-13 1.1437097575825064 1101 14-15 1.1597037462251194 1101 16-17 1.2080448561475876 1101 18-19 1.1400241089457026 1101 20-21 1.1933623458900868 1101 22-23 1.1945638747365663 1101 24-25 1.1445478776410383 1101 26-27 1.161245299230245 1101 28-29 1.2027278345578125 1101 30-31 1.0835471994173886 1101 32-33 1.1939512866929718 1101 34-35 1.2039941945244834 1101 36-37 1.2241131542323629 1101 38-39 1.1723107036091278 1101 40-41 1.0970689424214015 1101 42-43 1.1300887313193968 1101 44-45 1.1398950475241563 1101 46-47 1.1634275376284648 1101 48-49 1.138099740727096 1101 50-51 0.9054992294196857 1101 52-53 0.9495090146229366 1101 54-55 1.0172917885853856 1101 56-57 1.1492882098773975 1101 58-59 1.2683978952630852 1101 60-61 1.0739977222004704 1101 62-63 1.0900585652847425 1101 64-65 1.0527214783795635 1101 66-67 1.138669957561035 1101 68-69 0.9768747760833243 1101 70-71 0.8576180849514863 1101 72-73 0.8918112935761862 1101 74-75 1.0061877456548558 1101 76-77 1.20614022063371 1102 1 0.7377555139401224 1102 2 0.8362502293084972 1102 3 0.9221071453360068 1102 4 0.8148915302447932 1102 5 0.728237243224342 1102 6 0.6487835062869607 1102 7 0.6951210402782095 1102 8 0.7551327942049042 1102 9 0.7051525800277716 1102 10-11 0.851323148810387 1102 12-13 0.9628328653400828 1102 14-15 0.8413428588830243 1102 16-17 0.9088005778130324 1102 18-19 0.8467778657763034 1102 20-21 0.8094867990271837 1102 22-23 0.8298875539693285 1102 24-25 0.9851738973917037 1102 26-27 0.730426567343784 1102 28-29 0.818301103816232 1102 30-31 0.8871341464705509 1102 32-33 0.8177184254177732 1102 34-35 0.77964742260572 1102 36-37 0.7926263992380029 1102 38-39 0.8555783062340652 1102 40-41 0.9046240158711001 1102 42-43 0.9018528031872748 1102 44-45 0.8681156793962472 1102 46-47 0.9612511650095001 1102 48-49 0.806886278912085 1102 50-51 0.6402046977721554 1102 52-53 0.8255488146273606 1102 54-55 0.7803087330390461 1102 56-57 1.0032295807818024 1102 58-59 0.9793187162902797 1102 60-61 0.8475086788164745 1102 62-63 0.7148046646520143 1102 64-65 0.7170134222801394 1102 66-67 0.7109174776656815 1102 68-69 0.6408591528117178 1102 70-71 0.6680421145636615 1102 72-73 0.7150872927244336 1102 74-75 0.6580884226310282 1102 76-77 0.5918658371853347 1103 1 0.2924709193280819 1103 2 0.29542632777215516 1103 3 0.26841368232442875 1103 4 0.3949806661057025 1103 5 0.3002593684986863 1103 6 0.32934952169343035 1103 7 0.22867575635601867 1103 8 0.23433252147176376 1103 9 0.5499845021777929 1103 10-11 0.5009103171733429 1103 12-13 0.5159709438893785 1103 14-15 0.5141368875334535 1103 16-17 0.3095925528958716 1103 18-19 0.3214622995457219 1103 20-21 0.3420902103431942 1103 22-23 0.2212858358043306 1103 24-25 0.35952316391706773 1103 26-27 0.4250273118460868 1103 28-29 0.22733844862192853 1103 30-31 0.35226602800646134 1103 32-33 0.5216338539369474 1103 34-35 0.5389479858238602 1103 36-37 0.5647372165767308 1103 38-39 0.42166549342475435 1103 40-41 0.4317258631405494 1103 42-43 0.3515810786746414 1103 44-45 0.359758688121552 1103 46-47 0.3351562987939758 1103 48-49 0.21270559455093618 1103 50-51 0.24810978986666044 1103 52-53 0.3979495117913636 1103 54-55 0.40847364110592466 1103 56-57 0.3893075154872321 1103 58-59 0.3064329024481296 1103 60-61 0.1574413831242616 1103 62-63 0.1852669796023072 1103 64-65 0.13352638517337212 1103 66-67 0.12136253162057997 1103 68-69 0.13067435862572196 1103 70-71 -0.17461217869708534 1103 72-73 0.004157861752279501 1103 74-75 0.03962164550833336 1103 76-77 0.08804733786313435 1104 1 0.45622828869228016 1104 2 0.6343238794808101 1104 3 0.6012491697584181 1104 4 0.7367565581135764 1104 5 0.5982850828618353 1104 6 0.6730330701724228 1104 7 0.5638455781031695 1104 8 0.44323610814355163 1104 9 0.5076492027993353 1104 10-11 0.5498561485870184 1104 12-13 0.7180580958957492 1104 14-15 0.8308184377984773 1104 16-17 0.8241245524120231 1104 18-19 0.6793301596324071 1104 20-21 0.772398614806022 1104 22-23 0.9346088927345946 1104 24-25 0.914630210149646 1104 26-27 0.6766594950551408 1104 28-29 0.8197222987168757 1104 30-31 0.9597443917052004 1104 32-33 0.9849906220522442 1104 34-35 0.9444604916820083 1104 36-37 0.932581604424584 1104 38-39 0.8397932535280361 1104 40-41 0.81342488703649 1104 42-43 1.0722675507726862 1104 44-45 0.9753009655831093 1104 46-47 0.8819123904381669 1104 48-49 0.7908806759039599 1104 50-51 0.7892780873394436 1104 52-53 0.7407777215754088 1104 54-55 0.7334106309350581 1104 56-57 0.7981123151667759 1104 58-59 0.5993122078126376 1104 60-61 0.6534044687156388 1104 62-63 0.6643812479605273 1104 64-65 0.6104540535293417 1104 66-67 0.6752852246022414 1104 68-69 0.5081849330312629 1104 70-71 0.5821903748921287 1104 72-73 0.5201397727269885 1104 74-75 0.3198470279154577 1104 76-77 0.3527711936823792 1105 1 0.07869684990783554 1105 2 0.365929058970508 1105 3 0.21206426657360566 1105 4 0.23846118721468912 1105 5 0.2015930318572643 1105 6 0.5717593764736151 1105 7 0.3993482241524333 1105 8 0.3365117983246293 1105 9 0.2623479096057082 1105 10-11 0.48956183533301356 1105 12-13 0.5345253941361108 1105 14-15 0.44674855187949447 1105 16-17 0.4880816228777718 1105 18-19 0.5229709568306191 1105 20-21 0.3450542332377111 1105 22-23 0.4293836234161077 1105 24-25 0.3768728369830896 1105 26-27 0.24536056768009473 1105 28-29 0.43301427327141795 1105 30-31 0.47791192833535234 1105 32-33 0.49423468361518275 1105 34-35 0.4247849394209169 1105 36-37 0.45502314339472605 1105 38-39 0.3947084611441056 1105 40-41 0.4111267693602585 1105 42-43 0.48302424467037497 1105 44-45 0.4171011184831812 1105 46-47 0.3006561433599231 1105 48-49 0.13189915305030908 1105 50-51 0.10929362916613528 1105 52-53 0.25345364059003117 1105 54-55 0.17685649346447363 1105 56-57 0.10072950196055075 1105 58-59 0.2282841873260928 1105 60-61 0.35221168032984096 1105 62-63 0.21623264304447787 1105 64-65 0.3951264677232196 1105 66-67 0.31336211887133203 1105 68-69 0.2551145021475172 1105 70-71 0.13082928666724314 1105 72-73 -0.23534070668703322 1105 74-75 -0.5984274677338632 1105 76-77 -0.6188108958313094 1106 1 0.30358816608652006 1106 2 0.19663151820207503 1106 3 0.2504823167314285 1106 4 0.37041903261795994 1106 5 0.35599176542663713 1106 6 0.24376953096790288 1106 7 0.024915332810643065 1106 8 0.2820071020310806 1106 9 0.34405637445873793 1106 10-11 0.008339566514216301 1106 12-13 0.3547730780022462 1106 14-15 0.41246264780296826 1106 16-17 0.3758415676875231 1106 18-19 0.46495484473422266 1106 20-21 0.7639420087888098 1106 22-23 0.7006774891027518 1106 24-25 0.3538962928404956 1106 26-27 0.25155698607598254 1106 28-29 0.45538489310533947 1106 30-31 0.7434293849738722 1106 32-33 0.5910639651815117 1106 34-35 0.5236840155284028 1106 36-37 0.5608122775736248 1106 38-39 0.704544907199157 1106 40-41 0.47328535170066033 1106 42-43 0.7921971431830528 1106 44-45 0.6602056982497402 1106 46-47 0.6502587031120157 1106 48-49 0.4919977848186363 1106 50-51 0.5472032343189319 1106 52-53 0.5711057179076064 1106 54-55 0.5521368465028154 1106 56-57 0.5932335195804512 1106 58-59 0.4135054285305486 1106 60-61 0.22032602403322343 1106 62-63 0.22878410379345127 1106 64-65 0.3723473631882719 1106 66-67 0.355313753770524 1106 68-69 0.5454266760160174 1106 70-71 0.22992756309229634 1106 72-73 0.18985673292061733 1106 74-75 0.25429531009015705 1106 76-77 0.19846695059636943 1107 1 0.047706682622703056 1107 2 -0.0276350273113124 1107 3 0.19388713879791908 1107 4 -0.045653173906892164 1107 5 -5.803850855627957E-4 1107 6 -0.18263607527563863 1107 7 -0.057685738897994554 1107 8 -0.22058690586047902 1107 9 -0.08427513327723091 1107 10-11 3.1357664628473003E-4 1107 12-13 -0.07830215631732784 1107 14-15 -0.24294607336607754 1107 16-17 -0.05954589979402414 1107 18-19 0.038693626645866175 1107 20-21 -0.04217402763185163 1107 22-23 -0.02831263400059214 1107 24-25 0.12361655634887825 1107 26-27 -0.030330624548994933 1107 28-29 -0.016914908360952552 1107 30-31 0.12902666649342365 1107 32-33 0.3223742565717487 1107 34-35 0.18137439628992524 1107 36-37 0.1456088894308749 1107 38-39 0.1727226446616612 1107 40-41 0.18648185339970524 1107 42-43 0.16486001551155738 1107 44-45 0.1590004150124642 1107 46-47 0.10808351422492635 1107 48-49 0.2692948478888759 1107 50-51 0.344667961910762 1107 52-53 0.23624266882807987 1107 54-55 0.2253441306363051 1107 56-57 0.20345458282495343 1107 58-59 0.17276556370773477 1107 60-61 0.13091420349774197 1107 62-63 0.21059321891811322 1107 64-65 0.1713962373340756 1107 66-67 0.23053811553753434 1107 68-69 0.17393011145833626 1107 70-71 0.22011438082714463 1107 72-73 0.17927851125990557 1107 74-75 0.13552181589680146 1107 76-77 0.043508275482164294 1108 1 0.38083790001874007 1108 2 0.47904323450543984 1108 3 0.4286309128180008 1108 4 0.4234510593095422 1108 5 0.41836110952849026 1108 6 0.5009678773365849 1108 7 0.37545690394375697 1108 8 0.34408230084655145 1108 9 0.4287963462055089 1108 10-11 0.4327057216227601 1108 12-13 0.5134664464394589 1108 14-15 0.332763562251003 1108 16-17 0.3489971812402075 1108 18-19 0.44679752361084013 1108 20-21 0.2772406609921916 1108 22-23 0.2853583261867598 1108 24-25 0.368860418575764 1108 26-27 0.23274788483881537 1108 28-29 0.45908921367938405 1108 30-31 0.5093661383892041 1108 32-33 0.44089890001496457 1108 34-35 0.5481538473002203 1108 36-37 0.5177874555315256 1108 38-39 0.623687576831216 1108 40-41 0.4680084616643896 1108 42-43 0.5205351764741906 1108 44-45 0.4488092874334484 1108 46-47 0.3273326374847656 1108 48-49 0.39541911470677604 1108 50-51 0.3582006771649766 1108 52-53 0.47137671357794275 1108 54-55 0.5813503631281378 1108 56-57 0.49716253659274656 1108 58-59 0.560405481018293 1108 60-61 0.6061198763203386 1108 62-63 0.5853565701138166 1108 64-65 0.39475496909776453 1108 66-67 0.5254750447092462 1108 68-69 0.5388169640262781 1108 70-71 0.4626260519495382 1108 72-73 0.43599636254203844 1108 74-75 0.262984132645677 1108 76-77 0.2110328372304835 1109 1 -0.2482277987947512 1109 2 -0.3764791169666992 1109 3 -0.41192703806063236 1109 4 -0.20267430946903886 1109 5 -0.15748620234548127 1109 6 -0.41205605682471 1109 7 -0.2783422705977756 1109 8 -0.22978746840035313 1109 9 -0.22868773919812924 1109 10-11 -0.17652363067053045 1109 12-13 -0.3021411322433707 1109 14-15 -0.02934567292401624 1109 16-17 -0.09273896871939513 1109 18-19 -0.26499845629318486 1109 20-21 -0.21980830971761378 1109 22-23 -0.23692210871831776 1109 24-25 -0.4742134585446749 1109 26-27 -0.40216848701605556 1109 28-29 -0.10767937977031039 1109 30-31 -0.03301659595323514 1109 32-33 -0.041433790926348024 1109 34-35 -0.05485603517246318 1109 36-37 -0.14355338387527183 1109 38-39 0.022924392400952343 1109 40-41 0.09805769133984654 1109 42-43 0.06533157546878243 1109 44-45 0.001727076248194237 1109 46-47 -0.023777035413523606 1109 48-49 -0.1118240627711593 1109 50-51 -0.17615091957878448 1109 52-53 -0.021728671757561102 1109 54-55 0.07379275855602074 1109 56-57 0.01898858067630016 1109 58-59 0.11451707381424114 1109 60-61 -0.14266372025106833 1109 62-63 -0.12146316432047755 1109 64-65 -3.371117298200943E-6 1109 66-67 -0.11362328028231161 1109 68-69 -0.190552651864639 1109 70-71 -0.20414899016695287 1109 72-73 -0.22884014919795348 1109 74-75 -0.22848062742825448 1109 76-77 -0.07108046802858681 1110 1 -0.4698638251051648 1110 2 -0.5693686507404649 1110 3 -0.7483908656799088 1110 4 -0.7041759422154286 1110 5 -0.5892160921489733 1110 6 -0.47786123055514196 1110 7 -0.5477167045198286 1110 8 -0.8859798893936315 1110 9 -0.7499231543663498 1110 10-11 -0.5857130736498277 1110 12-13 -0.5953783877198973 1110 14-15 -0.5751245906273752 1110 16-17 -0.7469330513179315 1110 18-19 -0.7909401574795112 1110 20-21 -0.8497132086227523 1110 22-23 -0.8625370941334936 1110 24-25 -0.8999574903071945 1110 26-27 -0.8911500850429945 1110 28-29 -0.7064416669916014 1110 30-31 -0.5079804113629862 1110 32-33 -0.5651801363937281 1110 34-35 -0.5840689176635578 1110 36-37 -0.5472412568207474 1110 38-39 -0.8007816616361865 1110 40-41 -0.7140647846184933 1110 42-43 -0.7692093908151776 1110 44-45 -0.7476231134434101 1110 46-47 -0.7534491486379942 1110 48-49 -0.7672703153351605 1110 50-51 -0.7669154827664002 1110 52-53 -0.7156556682367743 1110 54-55 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2314 26-27 -0.3271223334773339 2314 28-29 -0.4980728670655523 2314 30-31 -0.505858193163526 2314 32-33 -0.6126941970521997 2314 34-35 -0.594441271149627 2314 36-37 -1.1838971479020373 2314 38-39 -0.7655210279171811 2314 40-41 -0.5721685239207375 2314 42-43 -0.3575221695729667 2314 44-45 -0.6219350655567695 2314 46-47 -0.4094785255685949 2314 48-49 -0.2163668078977672 2314 50-51 -0.27223444859907175 2314 52-53 -0.18170132048186005 2314 54-55 -0.2987280499014524 2314 56-57 -0.31869653463198233 2314 58-59 -0.27550306211752584 2314 60-61 -0.30631480316605675 2314 62-63 -0.32556507153633873 2314 64-65 -0.1506782176189212 2314 66-67 0.04768531371717799 2314 68-69 -0.0840106683646944 2314 70-71 -0.16536344953912518 2314 72-73 -0.05225285890297826 2314 74-75 -0.08250296350241015 2314 76-77 0.012331247590491756 2315 1 -1.2248169947850371 2315 2 -1.117780444677372 2315 3 -0.9631350546324242 2315 4 -1.0289943893609532 2315 5 -1.179900011462692 2315 6 -1.0105899043305513 2315 7 -1.468310639293513 2315 8 -1.5094047119333993 2315 9 -1.2380305584874591 2315 10-11 -1.3156204494857917 2315 12-13 -1.1740692117088045 2315 14-15 -1.1740987455602294 2315 16-17 -1.043968729347732 2315 18-19 -1.0682634746102337 2315 20-21 -0.9682163432367972 2315 22-23 -1.075502261267701 2315 24-25 -1.2145315324869372 2315 26-27 -0.9856976631758698 2315 28-29 -1.0900855880101759 2315 30-31 -1.2831910000814801 2315 32-33 -1.201246881102854 2315 34-35 -1.1978533611242739 2315 36-37 -1.3807024746899828 2315 38-39 -1.2307993568036864 2315 40-41 -1.2687789835676782 2315 42-43 -1.23718130715314 2315 44-45 -1.2390996101344385 2315 46-47 -1.1000035675138733 2315 48-49 -1.0625436205512493 2315 50-51 -1.165669495479218 2315 52-53 -1.1018109251085164 2315 54-55 -1.3103613588382217 2315 56-57 -1.4672985408750137 2315 58-59 -1.2871039207912212 2315 60-61 -1.2401578227005636 2315 62-63 -1.1093440018012757 2315 64-65 -1.168169792415842 2315 66-67 -1.2242052308832214 2315 68-69 -1.1478049054407968 2315 70-71 -1.0258536427716116 2315 72-73 -0.95710763900885 2315 74-75 -1.0195661749592517 2315 76-77 -1.1220982718178014 2316 1 -2.24510289482987 2316 2 -1.9277853419791526 2316 3 -2.1693767324952873 2316 4 -3.0011548391240552 2316 5 -2.508676490600301 2316 6 -2.5004517303713882 2316 7 -2.1479348880279474 2316 8 -1.848809768755693 2316 9 -2.0800992502784226 2316 10-11 -2.0622387823923347 2316 12-13 -2.7482505362972844 2316 14-15 -3.8848328619964896 2316 16-17 -2.934640845135185 2316 18-19 -2.6326787907837854 2316 20-21 -2.5759766377655993 2316 22-23 -2.8501119987299504 2316 24-25 -2.5638823736435157 2316 26-27 -2.4580913440278067 2316 28-29 -3.007195173311956 2316 30-31 -3.1731001091485176 2316 32-33 -3.325044400023721 2316 34-35 -3.2738121160217233 2316 36-37 -3.35082127987571 2316 38-39 -3.4173744920550675 2316 40-41 -3.034076744781906 2316 42-43 -3.2998923647560154 2316 44-45 -3.1653018401640978 2316 46-47 -3.179603152167818 2316 48-49 -2.8617429196700925 2316 50-51 -2.8769381023787197 2316 52-53 -2.9987248657146175 2316 54-55 -3.0103668451878036 2316 56-57 -2.746122664305144 2316 58-59 -2.814860012384358 2316 60-61 -2.4860065971076217 2316 62-63 -2.549174238469906 2316 64-65 -2.600424641681446 2316 66-67 -2.5593049728410975 2316 68-69 -2.641404980682161 2316 70-71 -2.527927433928575 2316 72-73 -2.466128887714259 2316 74-75 -2.616349912733291 2316 76-77 -3.0833658071646504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70030.0 3 5778.0 4 7549.0 5 9493.0 6 10609.0 7 10890.0 8 12996.0 9 13610.0 10 16517.0 11 19043.0 12 14859.0 13 12562.0 14 12304.0 15 12591.0 16 13009.0 17 13706.0 18 14445.0 19 15087.0 20 15950.0 21 17370.0 22 19427.0 23 21886.0 24 24897.0 25 29305.0 26 34225.0 27 40576.0 28 47787.0 29 56343.0 30 67040.0 31 80543.0 32 98291.0 33 122783.0 34 163278.0 35 217007.0 36 308090.0 37 426688.0 38 397386.0 39 106684.0 40 755.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.32650592934681 26.868031889197475 28.938373610639236 21.867088570816477 2 22.041529810761116 26.987132251813318 29.046847529542667 21.9244904078829 3 22.320660621661286 27.26493984697902 28.379316597212643 22.03508293414705 4 21.937809628681933 26.950684912399254 28.917313788836047 22.19419167008277 5 22.140601408501453 27.142265465755873 28.877069368522235 21.84006375722044 6 22.27022159956483 26.99279208632759 28.816699547316365 21.920286766791218 7 22.170682179306915 26.72633205847926 29.042534354611423 22.060451407602397 8 22.118831131343324 27.115531924044227 28.703258485433313 22.06237845917914 9 22.03050838263727 26.88170543176297 28.839100634267496 22.248685551332272 10-11 22.42829311537964 27.05972519173886 28.61250578296951 21.89947590991199 12-13 22.439150646949376 26.815348396400918 28.598091660137893 22.147409296511814 14-15 22.157393966090687 27.162238651406973 28.66645436058483 22.013913021917503 16-17 22.36429844762805 26.975118775117497 28.706280519447315 21.954302257807136 18-19 22.265560907081365 26.883820275259517 28.53306440317982 22.3175544144793 20-21 22.257534669037504 27.106289194991795 28.445977477305572 22.19019865866512 22-23 22.427976005401014 27.11232769500939 28.059151548965076 22.400544750624515 24-25 22.366113281079723 27.343270650828487 27.93211667419983 22.358499393891957 26-27 22.476521535639474 27.302414089228915 27.78305451436061 22.438009860770997 28-29 22.560768080880635 27.3322559218406 27.766591061901185 22.340384935377585 30-31 22.401854643407688 27.21934823262231 27.88171534369519 22.49708178027481 32-33 22.345309141513773 27.58346286226129 27.700232609676007 22.37099538654893 34-35 22.408027079790774 27.250666669766105 27.947807786795853 22.393498463647273 36-37 22.488455267418022 27.38945793850855 28.011892348213813 22.110194445859616 38-39 22.39820485957207 27.354697927289294 28.034268538005897 22.21282867513274 40-41 22.253397802470285 27.200939141754617 28.069044740961147 22.476618314813955 42-43 22.224648084732042 27.485588342583362 27.761820251853603 22.527943320830992 44-45 22.24152624379365 27.280747654571062 27.998494337978503 22.47923176365678 46-47 22.263262283185203 27.267142641828755 28.031416375462186 22.438178699523856 48-49 22.376602432161484 27.253478494597616 27.846728302087826 22.52319077115307 50-51 22.16974771420733 27.48093170753633 27.815217745097886 22.53410283315845 52-53 22.16109923720917 27.39984410538755 27.909979483369984 22.529077174033297 54-55 22.258021455386718 27.29853195804864 27.88456303699253 22.558883549572112 56-57 22.18419428922345 27.597430838270864 27.63685358083454 22.581521291671148 58-59 22.385143974556748 27.523465594868885 27.62116181985808 22.470228610716287 60-61 22.266313659636232 27.37189029762786 27.634223910194766 22.727572132541145 62-63 22.161868191886807 27.337288722413717 27.797144842762535 22.70369824293694 64-65 22.266772419570383 27.229519318955433 27.833213670332242 22.67049459114194 66-67 22.1836584411833 27.363769810972034 27.88464239557218 22.567929352272486 68-69 22.263809940467862 27.18829910451856 27.915158471423375 22.632732483590203 70-71 22.081895798230732 27.11422798308641 27.96537279786289 22.83850342081997 72-73 22.26305491189152 26.954614659258635 27.929947385916403 22.85238304293344 74-75 22.081363758399668 26.960804933815496 27.857932787134704 23.09989852065013 76-77 21.997543235202954 26.849062488551155 28.169093805808583 22.98430047043731 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8191.0 1 4973.5 2 1656.0 3 1558.5 4 1561.0 5 1650.5 6 1785.0 7 1934.5 8 2111.0 9 2183.0 10 2250.5 11 2394.0 12 2616.0 13 2762.0 14 2850.5 15 3147.0 16 3508.5 17 3662.0 18 4049.5 19 4869.0 20 6037.5 21 7999.0 22 9224.0 23 10463.0 24 13290.0 25 17491.0 26 20104.0 27 22869.5 28 29753.5 29 37797.0 30 41722.0 31 47671.5 32 59554.5 33 70772.0 34 80353.0 35 84650.0 36 91671.5 37 102564.0 38 107495.0 39 108555.0 40 109795.5 41 113030.5 42 116527.0 43 118029.0 44 117990.0 45 117751.0 46 118451.0 47 120007.5 48 120664.0 49 120102.5 50 117445.5 51 111662.0 52 107974.0 53 104924.5 54 97205.0 55 86664.5 56 75623.0 57 70452.0 58 66381.5 59 56362.0 60 45709.0 61 41005.0 62 36281.5 63 26778.0 64 19200.5 65 16403.0 66 14202.5 67 10752.0 68 8078.0 69 5624.5 70 4595.0 71 3881.5 72 2703.0 73 1980.5 74 1723.0 75 1562.5 76 1119.0 77 693.5 78 551.0 79 448.0 80 303.0 81 213.5 82 126.5 83 87.0 84 84.5 85 75.5 86 62.0 87 55.0 88 47.0 89 35.5 90 26.5 91 21.0 92 24.5 93 26.0 94 19.5 95 22.5 96 30.0 97 31.0 98 28.5 99 53.5 100 82.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0029441513851651184 2 0.008018938641173416 3 0.011815344374675804 4 0.01840094615728199 5 0.013481114237335015 6 0.01305498706316638 7 0.017045086966745424 8 0.018052296651144016 9 0.017781124813036703 10-11 0.02072527619820182 12-13 0.019640588845772567 14-15 0.008135155143219405 16-17 0.005636500349230588 18-19 0.011524803119560825 20-21 0.011757236123652808 22-23 0.015166253517001892 24-25 0.0224104154778687 26-27 0.013694177824419333 28-29 0.007844613888104427 30-31 0.007437856130943457 32-33 0.009336058997694652 34-35 0.01069191818823122 36-37 0.009045517742579674 38-39 0.016309049120454142 40-41 0.011970299710737127 42-43 0.01570859719321652 44-45 0.01799418840012102 46-47 0.017122564634776086 48-49 0.013616700156388674 50-51 0.025761324620194783 52-53 0.03097169779525674 54-55 0.02473474551878853 56-57 0.015166253517001892 58-59 0.016638329209584454 60-61 0.01995049951789521 62-63 0.026749164887585716 64-65 0.02053158202812517 66-67 0.02043473494308684 68-69 0.02967394685574317 70-71 0.05624878699025989 72-73 0.06804476194792804 74-75 0.07927902381237388 76-77 0.07672226076736206 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2581389.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.92582005375198 #Duplication Level Percentage of deduplicated Percentage of total 1 77.81727791343536 45.07629637486465 2 12.455101656618623 14.42943954624957 3 4.30955992123887 7.489043775256329 4 1.886198398972206 4.370383561781564 5 0.9848787755371491 2.8524955363262237 6 0.6056746519007792 2.105052053827404 7 0.3767623923321478 1.5276989378877168 8 0.25369315164207346 1.1756307080710366 9 0.17963690269305188 0.936505341036997 >10 0.9941509684140984 10.592760021704049 >50 0.0881900016191908 3.5505720110024757 >100 0.046930044057956775 4.824336409791206 >500 0.0018110683289764295 0.6493781562197247 >1k 6.707660477627629E-5 0.10183809893126605 >5k 6.707660477627629E-5 0.3185694670497713 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8199 0.3176197000916948 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2621 0.10153448395418127 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 3.8738834015330506E-5 0.0 0.0 0.0 2 0.0 3.8738834015330506E-5 0.0 3.8738834015330506E-5 0.0 3 0.0 3.8738834015330506E-5 0.0 1.5495533606132202E-4 0.0 4 0.0 3.8738834015330506E-5 0.0 6.198213442452881E-4 0.0 5 0.0 3.8738834015330506E-5 0.0 0.0012009038544752457 0.0 6 0.0 3.8738834015330506E-5 0.0 0.0027891960491037964 0.0 7 0.0 3.8738834015330506E-5 0.0 0.005927041604345568 0.0 8 0.0 3.8738834015330506E-5 0.0 0.009723447337847958 0.0 9 0.0 3.8738834015330506E-5 0.0 0.012822554059074398 0.0 10 0.0 3.8738834015330506E-5 0.0 0.0168901316306841 0.0 11 0.0 3.8738834015330506E-5 0.0 0.021926180052677068 0.0 12 0.0 3.8738834015330506E-5 0.0 0.028899170175436556 0.0 13 0.0 3.8738834015330506E-5 0.0 0.039281177691545134 0.0 14 0.0 3.8738834015330506E-5 0.0 0.05283976959691081 0.0 15 0.0 3.8738834015330506E-5 0.0 0.06721187701659843 0.0 16 0.0 3.8738834015330506E-5 0.0 0.08162272327030137 0.0 17 0.0 3.8738834015330506E-5 0.0 0.09529753167771304 0.0 18 0.0 3.8738834015330506E-5 0.0 0.11083180411786057 0.0 19 0.0 3.8738834015330506E-5 0.0 0.12741202507642205 0.0 20 0.0 3.8738834015330506E-5 0.0 0.14546432172756604 0.0 21 0.0 3.8738834015330506E-5 0.0 0.16336166304264874 0.0 22 0.0 3.8738834015330506E-5 0.0 0.18625631394570907 0.0 23 0.0 3.8738834015330506E-5 0.0 0.2078725833262635 0.0 24 0.0 3.8738834015330506E-5 0.0 0.23181318274773774 0.0 25 0.0 3.8738834015330506E-5 0.0 0.2567997306876259 0.0 26 0.0 3.8738834015330506E-5 0.0 0.2856214231950318 0.0 27 0.0 3.8738834015330506E-5 0.0 0.3106854488029507 0.0 28 0.0 3.8738834015330506E-5 0.0 0.33745398310754404 0.0 29 0.0 3.8738834015330506E-5 0.0 0.36236305337940156 0.0 30 0.0 3.8738834015330506E-5 0.0 0.3904874468745315 0.0 31 0.0 3.8738834015330506E-5 0.0 0.42229202960111784 0.0 32 0.0 3.8738834015330506E-5 0.0 0.45611103169650136 0.0 33 0.0 3.8738834015330506E-5 0.0 0.4908985046422682 0.0 34 0.0 3.8738834015330506E-5 0.0 0.5277391357908475 0.0 35 0.0 3.8738834015330506E-5 0.0 0.571049152219987 0.0 36 0.0 3.8738834015330506E-5 0.0 0.6179618802125523 0.0 37 0.0 3.8738834015330506E-5 0.0 0.6637511820186729 0.0 38 0.0 3.8738834015330506E-5 0.0 0.7096954391608549 0.0 39 0.0 3.8738834015330506E-5 0.0 0.7561433011452362 0.0 40 0.0 3.8738834015330506E-5 0.0 0.8073173008794877 0.0 41 0.0 3.8738834015330506E-5 0.0 0.8613192354968585 0.0 42 0.0 3.8738834015330506E-5 0.0 0.9197761360259922 0.0 43 0.0 3.8738834015330506E-5 0.0 0.9839276451553796 0.0 44 0.0 3.8738834015330506E-5 0.0 1.0537737628850203 0.0 45 0.0 3.8738834015330506E-5 0.0 1.126990159173995 0.0 46 0.0 3.8738834015330506E-5 0.0 1.2069858514156526 0.0 47 0.0 3.8738834015330506E-5 0.0 1.287330193163448 0.0 48 0.0 3.8738834015330506E-5 0.0 1.3697664319480714 0.0 49 0.0 3.8738834015330506E-5 0.0 1.452473842570802 0.0 50 0.0 3.8738834015330506E-5 0.0 1.533825394002996 0.0 51 0.0 3.8738834015330506E-5 0.0 1.6214526365456736 0.0 52 0.0 3.8738834015330506E-5 0.0 1.713031240157915 0.0 53 0.0 3.8738834015330506E-5 0.0 1.8148368959502035 0.0 54 0.0 3.8738834015330506E-5 0.0 1.9281479854450452 0.0 55 0.0 3.8738834015330506E-5 0.0 2.0516861271199343 0.0 56 0.0 3.8738834015330506E-5 0.0 2.181538698739322 0.0 57 0.0 3.8738834015330506E-5 0.0 2.3151101984241818 0.0 58 0.0 3.8738834015330506E-5 0.0 2.4509285504819305 0.0 59 0.0 3.8738834015330506E-5 0.0 2.5850423938430045 0.0 60 0.0 3.8738834015330506E-5 0.0 2.72105444007083 0.0 61 0.0 3.8738834015330506E-5 0.0 2.862993527903001 0.0 62 0.0 3.8738834015330506E-5 0.0 3.0103947913313336 0.0 63 0.0 3.8738834015330506E-5 0.0 3.1684104952798666 0.0 64 0.0 3.8738834015330506E-5 0.0 3.335336131051926 0.0 65 0.0 3.8738834015330506E-5 0.0 3.514813149044952 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCGT 250 0.0 26.97222 1 CGCGGAA 270 0.0 24.984442 59 CGGTATA 100 1.2552544E-5 24.846684 10 CAATTCG 280 0.0 24.088873 54 TCGCGCT 285 0.0 23.663507 37 CGGTTAC 235 0.0 22.654356 4 ATTCGCG 300 0.0 22.483383 56 AACTCGA 335 0.0 22.249468 13 CGCGCTT 340 0.0 20.881186 38 TAACGCG 155 7.629824E-7 20.616545 52 CTCGCGC 335 0.0 20.130081 36 TCGCGGA 345 0.0 19.55342 58 AATTCGC 335 0.0 19.074299 55 CGCTCCG 340 0.0 18.793066 25 TAGCGCG 95 0.0066542234 18.680271 5 ACGATCG 175 2.4415476E-6 18.259306 59 CGCGCTG 120 0.001221074 17.747631 26 ACTCGAG 420 0.0 17.746944 14 AACGCGA 185 4.1503845E-6 17.271646 53 TACCGCG 130 0.0020886983 16.37989 35 >>END_MODULE