FastQCFastQC Report
Thu 26 May 2016
SRR936128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936128_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581389
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87650.33954588014437187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62460.24196275725975433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44810.173588715222696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA11850.050.983208100-101
CGGTATC9100.037.28318100-101
GGTATCA83800.036.9336241
GCGTATC3200.036.27138100-101
CGTACAT17750.036.048695100-101
CGAGTAC11100.035.927856100-101
ACGTATC5200.031.478123100-101
AGGTATC20500.031.358072100-101
TACGCAG10150.031.080593100-101
GTATCAA163650.029.7872561
CCGTATC6250.029.523043100-101
GCGTACA5950.029.51104100-101
TCGTATC5150.029.47219100-101
TCGCATA1650.028.85928100-101
GACGCAG9150.028.297472100-101
CGACGCA2000.028.273077100-101
CCGGTAT4550.028.125908100-101
CCGAGTA5400.027.556604100-101
TCGAGTA5300.026.95348100-101
TACGTAC3100.026.8797298-99
Produced by FastQC (version 0.11.5)