FastQCFastQC Report
Thu 26 May 2016
SRR936127_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936127_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666177
Sequences flagged as poor quality0
Sequence length77
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162400.9746863628534063No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80360.48230170023953034No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA2050.027.70517259
CGGTTAC2100.027.0414644
ATTCGCG2100.025.35746456
TCGCGCT2150.024.76255437
AATTCGC2200.024.203455
AACTCGA2500.022.71892413
ACTCGAG2350.022.6557814
CTCGCGC2350.022.655136
TGCGTAA2850.022.41662637
TTACGGC2106.91216E-1121.9744958
CAATTCG2451.8189894E-1221.7375854
TTACGCG1355.330281E-621.03730456
TTGCGTA3150.020.2817136
CGTAAGT3000.020.11269439
CGATCGT2353.1468517E-1019.6338311
CGCGCTT2950.019.25043538
CCCGTCA4400.018.56820367
CGCTCCG2951.8189894E-1118.04836725
CTCGAGG3002.3646862E-1117.74702815
CCTCGCG3203.6379788E-1217.74596235
Produced by FastQC (version 0.11.5)