##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936127_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1666177 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.73783397562204 40.0 36.0 41.0 30.0 41.0 2 36.593288108046146 39.0 36.0 41.0 30.0 41.0 3 36.50164838429531 39.0 36.0 41.0 30.0 41.0 4 36.375329271740036 39.0 36.0 41.0 30.0 41.0 5 36.285811771498466 39.0 36.0 41.0 30.0 41.0 6 36.155056155498485 39.0 35.0 40.0 30.0 41.0 7 35.9541861398879 39.0 35.0 40.0 29.0 41.0 8 35.737589703854994 38.0 35.0 40.0 28.0 41.0 9 35.60573396463881 38.0 35.0 40.0 28.0 41.0 10-11 35.7672183087391 39.0 35.0 40.0 28.5 41.0 12-13 35.89401996306515 39.0 35.0 41.0 29.0 41.0 14-15 35.793504231543224 39.0 35.0 41.0 28.0 41.0 16-17 35.5702746466912 39.0 35.0 41.0 27.0 41.0 18-19 35.34223374827525 39.0 35.0 41.0 26.5 41.0 20-21 35.03067861337661 39.0 35.0 41.0 24.5 41.0 22-23 34.631572155899406 39.0 34.0 41.0 23.5 41.0 24-25 34.29563095637498 38.0 34.0 40.5 21.5 41.0 26-27 33.30041346147498 37.5 32.5 39.5 19.0 40.5 28-29 33.309524438279965 37.5 33.0 39.5 18.0 40.5 30-31 33.64640431358733 38.0 33.0 40.0 18.0 41.0 32-33 33.4660570275547 38.0 33.0 40.0 16.0 41.0 34-35 33.17421228356891 37.0 33.0 40.0 12.5 41.0 36-37 32.87481582088817 37.0 32.5 40.0 9.0 41.0 38-39 32.579415632312774 36.5 32.0 40.0 7.5 41.0 40-41 32.185719464378636 36.0 32.0 39.0 4.5 41.0 42-43 31.81711846940631 35.0 31.0 39.0 2.0 41.0 44-45 31.448579892772496 35.0 31.0 39.0 2.0 40.0 46-47 31.057661941078287 35.0 31.0 38.0 2.0 40.0 48-49 30.601900638407564 35.0 30.5 37.0 2.0 39.5 50-51 30.181176129546863 35.0 30.0 37.0 2.0 39.0 52-53 29.742157946004536 34.5 29.5 36.0 2.0 39.0 54-55 29.287560085153018 34.0 29.0 36.0 2.0 37.5 56-57 28.844360473107 34.0 29.0 35.0 2.0 37.0 58-59 28.424151215627152 34.0 28.5 35.0 2.0 37.0 60-61 28.084765904222664 34.0 28.0 35.0 2.0 36.0 62-63 27.73607155782369 34.0 27.0 35.0 2.0 36.0 64-65 27.357198244844334 34.0 26.5 35.0 2.0 35.5 66-67 26.971793813022266 33.5 25.5 35.0 2.0 35.0 68-69 26.604903620683757 33.0 25.0 35.0 2.0 35.0 70-71 26.17660038519317 33.0 24.5 35.0 2.0 35.0 72-73 25.689454961867796 33.0 21.5 35.0 2.0 35.0 74-75 25.108105561413943 33.0 18.0 35.0 2.0 35.0 76-77 23.465711325987577 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9906606330198073 1101 2 0.8061562853220536 1101 3 0.9798276116140343 1101 4 0.8800366961911266 1101 5 1.0608583823352333 1101 6 1.0794450268202596 1101 7 1.041759937113305 1101 8 1.169890837470362 1101 9 1.0237824618816163 1101 10-11 0.914875098154134 1101 12-13 0.922638083018704 1101 14-15 1.0633814491384257 1101 16-17 1.072600928176648 1101 18-19 1.1599154010927748 1101 20-21 1.191300687418618 1101 22-23 1.0794260099716695 1101 24-25 1.0558710151805002 1101 26-27 0.9438858631269511 1101 28-29 1.072988390131087 1101 30-31 1.1382660747610416 1101 32-33 0.9931257752354696 1101 34-35 0.9096714763547595 1101 36-37 0.948581217571018 1101 38-39 0.9721790690087246 1101 40-41 0.8836599339779525 1101 42-43 0.7563676349184476 1101 44-45 0.7597193834030485 1101 46-47 0.6783563945240836 1101 48-49 0.9211422839378365 1101 50-51 0.8001027447904043 1101 52-53 0.8147596987212431 1101 54-55 0.7824779342632802 1101 56-57 0.8345016419174982 1101 58-59 0.8656433036024751 1101 60-61 0.8580023646206456 1101 62-63 0.716147846385585 1101 64-65 0.8829943832541325 1101 66-67 0.9317670687744588 1101 68-69 0.9090290992547025 1101 70-71 0.9518333325444033 1101 72-73 1.0622053086394843 1101 74-75 1.0702936612804095 1101 76-77 1.0377049814378658 1102 1 0.8758910240402926 1102 2 0.7168446100564907 1102 3 0.7944275789076869 1102 4 0.6822630636127371 1102 5 1.0671920912523873 1102 6 0.9851716916539317 1102 7 0.9010471904454889 1102 8 0.9241970227428027 1102 9 0.9467185928651247 1102 10-11 0.91181921164155 1102 12-13 0.9653517569652266 1102 14-15 0.9765223002580825 1102 16-17 1.0195470184209867 1102 18-19 1.1828147823678563 1102 20-21 1.1231200944993631 1102 22-23 1.2660318644068767 1102 24-25 1.274464390888312 1102 26-27 1.0732771837263755 1102 28-29 1.061437839963702 1102 30-31 1.0095180986905845 1102 32-33 0.9957894552021216 1102 34-35 1.184639264202012 1102 36-37 1.1732225709666366 1102 38-39 1.1679687564475074 1102 40-41 1.204740069906947 1102 42-43 1.2749657728134167 1102 44-45 1.2386170538848553 1102 46-47 1.1568277144765737 1102 48-49 1.0234475419315743 1102 50-51 1.0844754030073318 1102 52-53 1.1321325168757816 1102 54-55 1.0914675059719379 1102 56-57 1.2598799662386178 1102 58-59 1.211494450211351 1102 60-61 1.0559808610993215 1102 62-63 1.2484166062581394 1102 64-65 1.1843343548966594 1102 66-67 1.1256622143985489 1102 68-69 1.137501317733065 1102 70-71 1.086045693744616 1102 72-73 1.018340173779734 1102 74-75 1.1500468182510843 1102 76-77 1.4033030312773 1103 1 0.6176905147753047 1103 2 0.3272209061499325 1103 3 0.5449197718455636 1103 4 0.6136395624642148 1103 5 0.669366780905321 1103 6 0.6009456524312213 1103 7 0.5008532975877458 1103 8 0.5163755656761637 1103 9 0.5414144953095459 1103 10-11 0.7018858672486843 1103 12-13 0.5474123666963848 1103 14-15 0.6049297982678041 1103 16-17 0.6639763511012831 1103 18-19 0.5592763298768659 1103 20-21 0.8225135319853791 1103 22-23 0.8066471077735713 1103 24-25 0.596189607229384 1103 26-27 0.2947313099401825 1103 28-29 0.3395158781085428 1103 30-31 0.5254980212563183 1103 32-33 0.4059740089018362 1103 34-35 0.5470653036002702 1103 36-37 0.3298682947469942 1103 38-39 0.3454930720513687 1103 40-41 0.2808399444778473 1103 42-43 0.24902196048836345 1103 44-45 0.229745731507478 1103 46-47 0.06234334747223613 1103 48-49 0.0690865325652048 1103 50-51 0.11592001451503364 1103 52-53 0.06092911721160377 1103 54-55 -0.1344600242684173 1103 56-57 -0.021893139619511004 1103 58-59 -0.039056112464141535 1103 60-61 0.009651088758854343 1103 62-63 -0.1561284652733157 1103 64-65 -0.07675611468568633 1103 66-67 -0.05443080853066462 1103 68-69 0.008648366462583823 1103 70-71 0.13328063588835803 1103 72-73 -0.037646718044900496 1103 74-75 -0.013556095495999898 1103 76-77 0.21540711549122804 1104 1 0.5425655799420994 1104 2 0.47973890745436165 1104 3 0.4470869085856748 1104 4 0.5772704926424908 1104 5 0.5478910774856729 1104 6 0.29427060951384476 1104 7 0.4717741834635163 1104 8 0.6380768170967457 1104 9 0.6480834305441121 1104 10-11 0.662251804701647 1104 12-13 0.7022135507143261 1104 14-15 0.9155628377779621 1104 16-17 0.9467694817031784 1104 18-19 1.0276782328563314 1104 20-21 1.0223318284217555 1104 22-23 0.9683946903309106 1104 24-25 0.9893566370850451 1104 26-27 0.7859637312655963 1104 28-29 0.7132142327424162 1104 30-31 0.9384288582924398 1104 32-33 0.8896115266522884 1104 34-35 1.020674660570343 1104 36-37 0.8460697959281731 1104 38-39 0.9897838064784139 1104 40-41 0.9194738739646482 1104 42-43 1.086537965889292 1104 44-45 0.9872449844069955 1104 46-47 0.8054280326757208 1104 48-49 0.8493755132147562 1104 50-51 0.7122152686273608 1104 52-53 0.6809701695653523 1104 54-55 0.7890672061809987 1104 56-57 0.6859144421961751 1104 58-59 0.7802123180140654 1104 60-61 0.6260070270625349 1104 62-63 0.6714538302992672 1104 64-65 0.7993962196488731 1104 66-67 0.7043678538867972 1104 68-69 0.6427732991144701 1104 70-71 0.5721087024359264 1104 72-73 0.4149103288032059 1104 74-75 0.3965078388551362 1104 76-77 0.43922206142732634 1105 1 -0.01538082373559746 1105 2 0.20898475934799876 1105 3 0.21517680205439405 1105 4 0.42392509916404464 1105 5 0.140061060692247 1105 6 -0.20016032670354633 1105 7 -0.08027571700787206 1105 8 0.20179912252964272 1105 9 -0.04651997871626179 1105 10-11 -0.09105403668798573 1105 12-13 0.012812862118941837 1105 14-15 -0.09430832615571916 1105 16-17 -0.05473933322546998 1105 18-19 -0.06554215008570452 1105 20-21 0.03375978850657191 1105 22-23 -2.7292299231618244E-4 1105 24-25 -0.05473616119281388 1105 26-27 -0.2651771195581958 1105 28-29 -0.029461822682378624 1105 30-31 0.002008516847688213 1105 32-33 0.17314878018117952 1105 34-35 0.04256447234129723 1105 36-37 0.21154276758683466 1105 38-39 0.14687043852079285 1105 40-41 0.07927663223654235 1105 42-43 0.13563442759833677 1105 44-45 0.031827969228064745 1105 46-47 -0.002219136756348661 1105 48-49 0.06480666340246799 1105 50-51 0.014224644417591747 1105 52-53 -0.12929003130738792 1105 54-55 -0.11415466806929331 1105 56-57 0.03069324227657333 1105 58-59 0.19168501734687027 1105 60-61 -0.01559139604107429 1105 62-63 -0.24584015843162987 1105 64-65 -0.18180162508648934 1105 66-67 -0.1431277208180184 1105 68-69 -0.35029406263152296 1105 70-71 -0.3845506373683918 1105 72-73 -0.38350953282932565 1105 74-75 -0.437768260660512 1105 76-77 -0.18501364308614754 1106 1 -0.14931604243066232 1106 2 0.012896367937251796 1106 3 -0.26676362120414154 1106 4 -0.09053609064741863 1106 5 -0.23137329632012893 1106 6 -0.1297414627460114 1106 7 0.13131908532211867 1106 8 0.11362614834565932 1106 9 0.03523819865250033 1106 10-11 -0.2692717752879261 1106 12-13 -0.07259863445155901 1106 14-15 -0.05731908857256229 1106 16-17 0.024838571506890617 1106 18-19 0.1069415210272524 1106 20-21 -0.14144270692651162 1106 22-23 -0.08666436820652734 1106 24-25 -0.05610106658006231 1106 26-27 -0.06249900974535194 1106 28-29 0.08082214484942085 1106 30-31 -0.1313666080963074 1106 32-33 -0.23794840039303722 1106 34-35 -0.0902283705222473 1106 36-37 -0.11591561313905174 1106 38-39 -0.0615124415674444 1106 40-41 -0.027971627164419033 1106 42-43 0.03647499906630003 1106 44-45 -0.17443861816379425 1106 46-47 -0.11416550562547911 1106 48-49 -0.31924746750436483 1106 50-51 -0.18549078487272652 1106 52-53 -0.05534214587671826 1106 54-55 0.20616704116449824 1106 56-57 0.07425798049737153 1106 58-59 0.04395102741132462 1106 60-61 -0.039541435678479786 1106 62-63 -0.1326053585143505 1106 64-65 0.0961371437412808 1106 66-67 0.12535860427762557 1106 68-69 0.15039011342748054 1106 70-71 -0.16169579643501208 1106 72-73 -0.1950916253395789 1106 74-75 -0.5174284560901512 1106 76-77 -0.4651660747547126 1107 1 0.1975492826467402 1107 2 0.27601556298369445 1107 3 0.29782616207759105 1107 4 0.1814066338107878 1107 5 0.3759796448563435 1107 6 0.1760247911239574 1107 7 0.21924478191655083 1107 8 0.17720973967692544 1107 9 0.039108167692752716 1107 10-11 0.23573983461918857 1107 12-13 0.37571792901592715 1107 14-15 0.4476925213345595 1107 16-17 0.618472472435684 1107 18-19 0.6803741835659451 1107 20-21 0.5370402652097113 1107 22-23 0.47688052343433895 1107 24-25 0.22470388698031485 1107 26-27 0.3644359747747714 1107 28-29 0.4285582129608727 1107 30-31 0.5744770451544667 1107 32-33 0.6479262126100522 1107 34-35 0.7052244158554615 1107 36-37 0.8969326840745708 1107 38-39 0.6783869392375053 1107 40-41 0.5282396731451797 1107 42-43 0.7060957421003593 1107 44-45 0.7809406388021465 1107 46-47 0.6904784572228202 1107 48-49 0.7749862786256649 1107 50-51 0.7018350665204629 1107 52-53 0.7974442318322623 1107 54-55 0.7109464529292033 1107 56-57 0.6965296523239957 1107 58-59 0.5168418942843864 1107 60-61 0.5110390596775609 1107 62-63 0.5563458798757459 1107 64-65 0.46962785581558464 1107 66-67 0.5128005547420251 1107 68-69 0.14652014438723526 1107 70-71 0.4032809837507436 1107 72-73 0.6288054334839508 1107 74-75 0.7097193767271897 1107 76-77 0.6439283524898691 1108 1 0.0068938420366038144 1108 2 0.13887518693596945 1108 3 -0.011035123129687463 1108 4 -0.24141863236756933 1108 5 -0.21738099850241355 1108 6 -0.23344813797066166 1108 7 -0.483892724690719 1108 8 -0.1991947759162187 1108 9 -0.1696719425541744 1108 10-11 -0.030062853593442185 1108 12-13 0.06571169930967358 1108 14-15 0.06982308087544453 1108 16-17 0.0907483917893046 1108 18-19 0.032254422182575126 1108 20-21 -0.06665130769673056 1108 22-23 -0.011022519682782672 1108 24-25 0.05984886923508981 1108 26-27 -0.1576851458172399 1108 28-29 0.12364146448997815 1108 30-31 0.13829000294348504 1108 32-33 0.09218638779694288 1108 34-35 0.26987272062024914 1108 36-37 0.10677373217545494 1108 38-39 0.06310244931830766 1108 40-41 0.09360250634007627 1108 42-43 0.18965214925885476 1108 44-45 0.2067712663034733 1108 46-47 0.18039482813575702 1108 48-49 0.2477454721618706 1108 50-51 0.2007335411863238 1108 52-53 0.09842871420030619 1108 54-55 0.1011365533596198 1108 56-57 -0.003991377654106287 1108 58-59 -0.05267124473245133 1108 60-61 0.0386036220878907 1108 62-63 0.14660677242275 1108 64-65 0.04156397300956982 1108 66-67 -0.1153586614476616 1108 68-69 -0.014885881437731285 1108 70-71 0.005602021280004976 1108 72-73 -0.03726909645639509 1108 74-75 0.09371563890343282 1108 76-77 0.221692076721542 1109 1 0.03742737862197032 1109 2 0.042731894253037694 1109 3 -0.25972414751993256 1109 4 0.0753191725104756 1109 5 -0.13597855947802628 1109 6 0.11147564251714925 1109 7 -0.2828256515199854 1109 8 -0.11117648323328666 1109 9 -0.010617064505389351 1109 10-11 -0.2012290121300353 1109 12-13 0.026184260285283756 1109 14-15 0.09101972721690998 1109 16-17 0.2791477820332062 1109 18-19 0.00934283681672099 1109 20-21 -0.015439417452824955 1109 22-23 0.08012839495136603 1109 24-25 0.12083972289362777 1109 26-27 0.03516546393885278 1109 28-29 0.06851951327047345 1109 30-31 -0.08033042388577627 1109 32-33 -0.00579379512988254 1109 34-35 0.14802820842512432 1109 36-37 0.2825054394925317 1109 38-39 0.15103690053781804 1109 40-41 0.17666653073031569 1109 42-43 0.29177105169787865 1109 44-45 0.26949230288883896 1109 46-47 0.07711738911136123 1109 48-49 0.18798937460089604 1109 50-51 0.2927000046009596 1109 52-53 0.29202627517591395 1109 54-55 0.37679204116449583 1109 56-57 0.4582875857605302 1109 58-59 0.5242799747797449 1109 60-61 0.4014618537952046 1109 62-63 0.3108979309593316 1109 64-65 0.31269964374127923 1109 66-67 0.45243097269868215 1109 68-69 0.3823473502695869 1109 70-71 0.33085354567024794 1109 72-73 0.27087876939726385 1109 74-75 0.36706167548879876 1109 76-77 0.5261661620873923 1110 1 -0.4376389866055703 1110 2 -0.5219566996792793 1110 3 -0.5150494100636891 1110 4 -0.5602015392748214 1110 5 -0.3181401697463997 1110 6 -0.4294366970394492 1110 7 -0.405805523165256 1110 8 -0.24300116234647362 1110 9 -0.3867387097796069 1110 10-11 -0.25090484351860454 1110 12-13 -0.3397572507998987 1110 14-15 -0.3256652932031585 1110 16-17 -0.45890866487460613 1110 18-19 -0.35236150974103 1110 20-21 -0.6701444203699793 1110 22-23 -0.6605159311863247 1110 24-25 -0.536611414795992 1110 26-27 -0.4628122198417657 1110 28-29 -0.49063159524761346 1110 30-31 -0.5571642191016437 1110 32-33 -0.4606016276853069 1110 34-35 -0.2017231042936629 1110 36-37 -0.3575295663417748 1110 38-39 -0.2548006869767647 1110 40-41 -0.35506698735602527 1110 42-43 -0.25850826568835217 1110 44-45 -0.21777906525585067 1110 46-47 -0.39173106479762154 1110 48-49 -0.45973524616923456 1110 50-51 -0.4640539335554017 1110 52-53 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-0.38155288453592817 2314 2 -0.39595990736638953 2314 3 -0.31954702201385743 2314 4 -0.22945309469599806 2314 5 -0.2001992072513019 2314 6 -0.3126868068755684 2314 7 -0.3327497405888096 2314 8 -0.504946726148269 2314 9 -0.27832455438393566 2314 10-11 -0.43933756476160823 2314 12-13 -0.5117939785811174 2314 14-15 -0.6178707080057606 2314 16-17 -1.0630409831489729 2314 18-19 -0.7588732563006886 2314 20-21 -0.4052078891127451 2314 22-23 -0.3016441252915456 2314 24-25 -0.3846941839889624 2314 26-27 -0.28749900974534626 2314 28-29 -0.21049870535301096 2314 30-31 -0.47569859190196695 2314 32-33 -0.4845577121339346 2314 34-35 -0.38008160938864677 2314 36-37 -0.5531119694143456 2314 38-39 -0.544559000271903 2314 40-41 -0.4425718295935681 2314 42-43 -0.1309339078162921 2314 44-45 -0.41624023759699114 2314 46-47 -0.20839627485624845 2314 48-49 -0.28863005859748725 2314 50-51 -0.16866386179580317 2314 52-53 -0.09527129567429071 2314 54-55 -0.16582182523226408 2314 56-57 -0.14560537982651667 2314 58-59 -0.06847306165750311 2314 60-61 -0.2497641077432533 2314 62-63 -0.237995034627712 2314 64-65 -0.3098851234651967 2314 66-67 -0.33630637547945597 2314 68-69 -0.18068276106644632 2314 70-71 -0.11539012841882013 2314 72-73 -0.012374014003544431 2314 74-75 0.07900373014467021 2314 76-77 0.0434371641116833 2315 1 -1.2318323909171056 2315 2 -1.1063249694744144 2315 3 -0.9462396785820602 2315 4 -1.0396382423191781 2315 5 -0.7593002027645994 2315 6 -0.9248382352383615 2315 7 -1.0355936875921259 2315 8 -1.0364094491651556 2315 9 -1.0251035374512796 2315 10-11 -1.1425597987031821 2315 12-13 -1.204969296828942 2315 14-15 -1.4101820513402075 2315 16-17 -1.3977823281872332 2315 18-19 -1.2562921832233584 2315 20-21 -1.301599863344613 2315 22-23 -1.5898997864113582 2315 24-25 -1.5306870807135837 2315 26-27 -1.429374866722462 2315 28-29 -1.4294213684930561 2315 30-31 -1.494272683404816 2315 32-33 -1.4778347520437123 2315 34-35 -1.2264119218353997 2315 36-37 -1.4085166045956434 2315 38-39 -1.4484639762156917 2315 40-41 -1.3044093710732874 2315 42-43 -1.6590067539133457 2315 44-45 -1.5522455478125643 2315 46-47 -1.457320236139136 2315 48-49 -1.4451769580644367 2315 50-51 -1.3079099653389221 2315 52-53 -1.2737552879503404 2315 54-55 -1.318093980879592 2315 56-57 -1.4175196537184291 2315 58-59 -1.1280897646992116 2315 60-61 -1.2755753456035919 2315 62-63 -1.389042575052688 2315 64-65 -1.446974078703608 2315 66-67 -1.3300752898263717 2315 68-69 -1.3253323841993527 2315 70-71 -1.4156312990191324 2315 72-73 -2.396274912967467 2315 74-75 -3.168432437737657 2315 76-77 -2.8276871695759347 2316 1 -3.141340717146406 2316 2 -3.0785890968382716 2316 3 -2.6492202499698223 2316 4 -2.463770471142084 2316 5 -3.0272090717274693 2316 6 -2.8189851035853053 2316 7 -3.301074537934241 2316 8 -2.847821806173158 2316 9 -2.583927198135683 2316 10-11 -2.5199699642458455 2316 12-13 -2.704053768667947 2316 14-15 -2.6865895433844216 2316 16-17 -3.911986683445633 2316 18-19 -3.71509062643495 2316 20-21 -3.0436903918403573 2316 22-23 -3.087153759837051 2316 24-25 -3.0538944085094926 2316 26-27 -2.844482364568936 2316 28-29 -2.9542477473085924 2316 30-31 -2.946653502950369 2316 32-33 -2.9693969410096166 2316 34-35 -3.03516082275528 2316 36-37 -2.866592556053128 2316 38-39 -2.9177499869900245 2316 40-41 -3.1271210527886097 2316 42-43 -3.379374549638424 2316 44-45 -3.080383114705814 2316 46-47 -2.7823550496414207 2316 48-49 -2.800206616490417 2316 50-51 -3.0615274452876804 2316 52-53 -4.2855349720401215 2316 54-55 -3.511079618078263 2316 56-57 -3.302527419807401 2316 58-59 -3.4493447468238188 2316 60-61 -3.3816276506591336 2316 62-63 -3.320446027837992 2316 64-65 -3.293367310601706 2316 66-67 -3.45932153286925 2316 68-69 -3.4279066864787424 2316 70-71 -3.330015747202804 2316 72-73 -3.422231614789819 2316 74-75 -3.4469186975624275 2316 76-77 -3.4048492889148356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38195.0 3 4189.0 4 6144.0 5 7269.0 6 8049.0 7 8415.0 8 10283.0 9 11590.0 10 15518.0 11 17667.0 12 12450.0 13 9303.0 14 8583.0 15 8766.0 16 8725.0 17 9180.0 18 9612.0 19 10221.0 20 10907.0 21 11438.0 22 13056.0 23 14722.0 24 17031.0 25 19838.0 26 23281.0 27 27115.0 28 32032.0 29 37873.0 30 44518.0 31 53934.0 32 65965.0 33 82977.0 34 113261.0 35 150193.0 36 202298.0 37 259570.0 38 223594.0 39 58014.0 40 401.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.102308741332617 26.15040911972034 30.29472706144479 21.452555077502254 2 21.810377479106805 26.698992120520558 30.015612497921634 21.475017902451 3 21.873099813620417 26.621787503263896 29.675831580084694 21.829281103030993 4 21.74994567585835 26.55484097132766 30.132610932298316 21.562602420515677 5 22.025193670489454 26.658339205773917 29.818412532248402 21.498054591488224 6 21.76439764492482 26.673525331943992 29.960222542480007 21.60185448065118 7 21.949699805152477 26.420388038306807 29.65878272520016 21.971129431340557 8 21.5843994782378 26.852925127844347 29.575428914101927 21.98724647981593 9 21.691643230815867 26.67028834350304 29.74515200537849 21.892916420302598 10-11 22.230969820884663 26.602061165024065 29.506877553495087 21.66009146059619 12-13 22.26379768931511 26.354587544487618 29.237211066844427 22.144403699352846 14-15 22.048090552203135 26.56774774023143 29.50011239195592 21.884049315609516 16-17 22.053401277861308 26.616218454862206 29.535882453565737 21.794497813710752 18-19 22.246977069034806 26.589486770296865 29.06665890305581 22.096877257612515 20-21 21.971652752977462 26.850163479469842 28.99213023369175 22.186053533860946 22-23 22.226584366215995 27.05070542171477 28.47010305565185 22.25260715641738 24-25 22.322046814675726 27.267941338636682 27.878344393143152 22.531667453544447 26-27 22.294737227572217 27.374352820336114 27.7911231668183 22.539786785273375 28-29 22.59978667838315 27.253277103819922 27.732470443191566 22.414465774605365 30-31 22.156020365749168 27.248147920283667 27.863021879825272 22.73280983414189 32-33 22.30287122107253 27.517911930893096 27.631117314931558 22.54809953310282 34-35 22.36622107455317 27.054137025802444 27.76191629518984 22.81772560445454 36-37 22.178424400888723 27.371287376833635 27.989809053303787 22.460479168973855 38-39 22.248887117454128 27.274896395076905 28.12111331473327 22.3551031727357 40-41 22.089121676454628 27.301001445385094 27.964960215895395 22.644916662264887 42-43 22.024378524112755 27.547708236853392 27.76149228045417 22.666420958579682 44-45 22.200639692573006 27.10875103881764 28.10130396561975 22.589305302989604 46-47 21.952158791534185 27.33677856309486 28.0513080497052 22.659754595665756 48-49 22.16109019515324 27.329257798160455 27.70541486684874 22.80423713983756 50-51 21.84284358266055 27.615846406286305 27.72796890469377 22.81334110635937 52-53 21.92511265943513 27.334596796152095 27.997989827253033 22.74230071715975 54-55 21.825009342820735 27.41248140816195 27.92679430935918 22.835714939658136 56-57 21.8451182857407 27.608820540280306 27.798034503934655 22.748026670044343 58-59 21.921523724914348 27.42782880904967 27.692907962128977 22.957739503907007 60-61 21.733920939673904 27.250849338882837 27.755605306465842 23.259624414977417 62-63 21.943476091880466 27.169218134763863 27.734901454159722 23.152404319195952 64-65 21.826001918650654 27.06426038898901 27.745903656842486 23.36383403551785 66-67 21.675355046929862 27.109967668839584 27.953141279456673 23.26153600477388 68-69 21.92679899068288 26.940120955939197 27.923139390536978 23.209940662840946 70-71 21.677627746825472 26.579158174631868 28.25379544414593 23.489418634396735 72-73 21.9494848317838 26.427521290766315 28.021696984077167 23.60129689337272 74-75 21.747700828362557 26.523700540027512 27.85544803061157 23.87315060099836 76-77 21.634620005574725 26.241788093155584 28.477355081169925 23.64623682009977 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 18760.0 1 11004.0 2 2963.0 3 2671.5 4 2665.0 5 2661.0 6 2698.0 7 2732.5 8 2735.0 9 2744.0 10 2819.0 11 2959.0 12 3098.0 13 3172.0 14 3269.0 15 3584.5 16 4111.0 17 4419.0 18 4728.5 19 5326.5 20 6048.0 21 7166.5 22 7852.0 23 8845.0 24 10800.0 25 13167.0 26 14572.0 27 15691.0 28 19556.0 29 23632.0 30 24962.0 31 28098.0 32 33707.0 33 38966.5 34 44197.0 35 46641.0 36 49586.5 37 55146.0 38 57856.0 39 57952.0 40 60480.5 41 64222.5 42 68344.0 43 71252.0 44 69833.0 45 68668.0 46 70312.0 47 73190.5 48 74679.0 49 74718.0 50 74481.0 51 72096.5 52 69988.0 53 69236.0 54 65590.0 55 59330.5 56 53320.5 57 50676.0 58 49308.0 59 43684.5 60 36837.0 61 34245.0 62 30313.0 63 22024.5 64 15642.5 65 13617.0 66 11890.0 67 9111.0 68 6798.0 69 4813.0 70 4089.0 71 3524.5 72 2570.0 73 1979.0 74 1778.0 75 1550.0 76 1048.0 77 587.0 78 400.0 79 349.5 80 265.5 81 185.0 82 115.0 83 92.0 84 90.0 85 64.0 86 41.0 87 42.0 88 40.0 89 35.5 90 32.0 91 31.0 92 30.0 93 25.0 94 27.0 95 29.5 96 26.0 97 22.5 98 26.0 99 55.0 100 77.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009662838942081184 2 0.012243597168848206 3 0.01332391456609952 4 0.015064425928337745 5 0.012183579535667578 6 0.009482786042539298 7 0.015544566993782774 8 0.017045007823298482 9 0.01692497255693723 10-11 0.019385695517342993 12-13 0.015844655159685916 14-15 0.007952336396433272 16-17 0.006721974916230388 18-19 0.017135034273069425 20-21 0.022296550726603474 22-23 0.01977581013301708 24-25 0.018635475102585137 26-27 0.023496903390216047 28-29 0.008882609710733014 30-31 0.008672547994600814 32-33 0.010713147522742182 34-35 0.011493376754090352 36-37 0.011313323854548467 38-39 0.013984108531086434 40-41 0.011103262138416265 42-43 0.014614293679483033 44-45 0.013233888116328578 46-47 0.02373697392293856 48-49 0.02301676232477102 50-51 0.03529036831020954 52-53 0.038921435117637564 54-55 0.027578102446498783 56-57 0.020285960015052423 58-59 0.015574575810373088 60-61 0.019355686700752682 62-63 0.024247123804973902 64-65 0.02538745883540584 66-67 0.026797873215150612 68-69 0.04168224624394647 70-71 0.05347571116393996 72-73 0.08579520663170839 74-75 0.0868455152123694 76-77 0.0912868200677359 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1666177.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.343595811947765 #Duplication Level Percentage of deduplicated Percentage of total 1 74.60346804582191 36.81203373422598 2 12.134848798535089 11.975541487080307 3 5.0290960378603655 7.444610465749495 4 2.547411137609378 5.027937021642048 5 1.477120922641707 3.6443228886101875 6 0.9513200846620185 2.816493224721034 7 0.6188481919303758 2.1375336536096996 8 0.45393034657246767 1.7918844438439372 9 0.3271851302534063 1.4530041740613204 >10 1.6639506918966702 14.827357265920133 >50 0.12697025617083194 4.337438372457174 >100 0.0630405572328467 5.354131904238037 >500 0.0024433033156301093 0.8504399128147396 >1k 1.2216516578150547E-4 0.06389752676379529 >5k 1.2216516578150547E-4 0.4844155896923198 >10k+ 1.2216516578150547E-4 0.9789583345698448 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16240 0.9746863628534063 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8036 0.48230170023953034 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.2003526636125694E-4 0.0 2 0.0 0.0 0.0 0.001020299764070684 0.0 3 0.0 0.0 0.0 0.0016804937290575972 0.0 4 0.0 0.0 0.0 0.004081199056282736 0.0 5 0.0 0.0 0.0 0.007562221780759187 0.0 6 0.0 0.0 0.0 0.01410414379744769 0.0 7 0.0 0.0 0.0 0.027248005464005326 0.0 8 0.0 0.0 0.0 0.03775109127061531 0.0 9 0.0 0.0 0.0 0.05131507636943734 0.0 10 0.0 0.0 0.0 0.06781992549411017 0.0 11 0.0 0.0 0.0 0.0860052683478406 0.0 12 0.0 0.0 0.0 0.10971223345418885 0.0 13 0.0 0.0 0.0 0.14302201986943763 0.0 14 0.0 0.0 0.0 0.18185342853730427 0.0 15 0.0 0.0 0.0 0.231488011177684 0.0 16 0.0 0.0 0.0 0.2811826114512444 0.0 17 0.0 0.0 0.0 0.3266159597689801 0.0 18 0.0 0.0 0.0 0.3695885851263101 0.0 19 0.0 0.0 0.0 0.4121410870513757 0.0 20 0.0 0.0 0.0 0.4605152993949622 0.0 21 0.0 0.0 0.0 0.5155514690215985 0.0 22 0.0 0.0 0.0 0.5732284145081825 0.0 23 0.0 0.0 0.0 0.639067758107332 0.0 24 0.0 0.0 0.0 0.7031665903442431 0.0 25 0.0 0.0 0.0 0.7732671858992172 0.0 26 0.0 0.0 0.0 0.8497296505713379 0.0 27 0.0 0.0 0.0 0.9198902637594926 0.0 28 0.0 0.0 0.0 0.990891123812176 0.0 29 0.0 0.0 0.0 1.0606916312012469 0.0 30 0.0 0.0 0.0 1.1378142898383545 0.0 31 6.001763318062847E-5 0.0 0.0 1.2176377419685904 0.0 32 6.001763318062847E-5 0.0 0.0 1.3049033806132242 0.0 33 6.001763318062847E-5 0.0 0.0 1.4006315055363265 0.0 34 6.001763318062847E-5 0.0 0.0 1.500080723716628 0.0 35 6.001763318062847E-5 0.0 0.0 1.6085926045072043 0.0 36 6.001763318062847E-5 0.0 0.0 1.7183648555945736 0.0 37 6.001763318062847E-5 0.0 0.0 1.8361194518949668 0.0 38 6.001763318062847E-5 0.0 0.0 1.9501529549381609 0.0 39 6.001763318062847E-5 0.0 0.0 2.063346211116826 0.0 40 6.001763318062847E-5 0.0 0.0 2.1797804194872454 0.0 41 6.001763318062847E-5 0.0 0.0 2.304737131769314 0.0 42 6.001763318062847E-5 0.0 0.0 2.436655889500335 0.0 43 6.001763318062847E-5 0.0 0.0 2.5803981209679403 0.0 44 6.001763318062847E-5 0.0 0.0 2.7396249017961476 0.0 45 6.001763318062847E-5 0.0 0.0 2.901912581916567 0.0 46 6.001763318062847E-5 0.0 0.0 3.0793247055985047 0.0 47 6.001763318062847E-5 0.0 0.0 3.263338768930312 0.0 48 6.001763318062847E-5 0.0 0.0 3.4455523032667 6.001763318062847E-5 49 6.001763318062847E-5 0.0 0.0 3.6312468603275643 6.001763318062847E-5 50 6.001763318062847E-5 0.0 0.0 3.8197622461479184 6.001763318062847E-5 51 6.001763318062847E-5 0.0 0.0 4.013139060255903 6.001763318062847E-5 52 6.001763318062847E-5 0.0 0.0 4.212697690581493 6.001763318062847E-5 53 6.001763318062847E-5 0.0 0.0 4.431942104590329 6.001763318062847E-5 54 6.001763318062847E-5 0.0 0.0 4.66793143825656 6.001763318062847E-5 55 6.001763318062847E-5 0.0 0.0 4.9127433640003435 6.001763318062847E-5 56 6.001763318062847E-5 0.0 0.0 5.176580879462386 6.001763318062847E-5 57 6.001763318062847E-5 0.0 0.0 5.437357495632217 6.001763318062847E-5 58 6.001763318062847E-5 0.0 0.0 5.718180001284377 6.001763318062847E-5 59 6.001763318062847E-5 0.0 0.0 5.992040461487585 6.001763318062847E-5 60 6.001763318062847E-5 0.0 0.0 6.268241609384837 6.001763318062847E-5 61 6.001763318062847E-5 0.0 0.0 6.554225631490532 6.001763318062847E-5 62 6.001763318062847E-5 0.0 0.0 6.839849547797143 6.001763318062847E-5 63 6.001763318062847E-5 0.0 0.0 7.157762950754932 1.2003526636125694E-4 64 6.001763318062847E-5 0.0 0.0 7.473575736551399 1.2003526636125694E-4 65 6.001763318062847E-5 0.0 0.0 7.817056651244136 1.2003526636125694E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAA 205 0.0 27.705172 59 CGGTTAC 210 0.0 27.041464 4 ATTCGCG 210 0.0 25.357464 56 TCGCGCT 215 0.0 24.762554 37 AATTCGC 220 0.0 24.2034 55 AACTCGA 250 0.0 22.718924 13 ACTCGAG 235 0.0 22.65578 14 CTCGCGC 235 0.0 22.6551 36 TGCGTAA 285 0.0 22.416626 37 TTACGGC 210 6.91216E-11 21.97449 58 CAATTCG 245 1.8189894E-12 21.73758 54 TTACGCG 135 5.330281E-6 21.037304 56 TTGCGTA 315 0.0 20.28171 36 CGTAAGT 300 0.0 20.112694 39 CGATCGT 235 3.1468517E-10 19.633831 1 CGCGCTT 295 0.0 19.250435 38 CCCGTCA 440 0.0 18.568203 67 CGCTCCG 295 1.8189894E-11 18.048367 25 CTCGAGG 300 2.3646862E-11 17.747028 15 CCTCGCG 320 3.6379788E-12 17.745962 35 >>END_MODULE