FastQCFastQC Report
Thu 26 May 2016
SRR936127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666177
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109880.6594737533887456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82520.49526550900654615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62640.37595045424345674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19500.11703438470222552No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19490.11697436706904489No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12100.050.42152100-101
CGTACAT13500.049.381214100-101
CGGTATC10350.044.857155100-101
GGTATCA82150.043.313941
TACGCAG7800.042.352184100-101
CCGTACA6400.041.386383100-101
ACGTACA4750.040.725574100-101
ACGTATC3300.039.681328100-101
CGAGTAC10250.038.90706100-101
AGTATCA26050.038.2723100-101
TTTACGC2200.037.876598-99
TCGTATC5200.037.201244100-101
AGGTATC16600.037.111607100-101
TCGAGTA4100.037.019775100-101
TACCGTA1550.034.5601298-99
CCGGTAT4550.034.012566100-101
TCGGTAT3600.033.894466100-101
TGTATCA24950.033.876247100-101
ACGGTAT3100.032.641094100-101
TCGTACA6450.032.298756100-101
Produced by FastQC (version 0.11.5)