FastQCFastQC Report
Thu 26 May 2016
SRR936126_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936126_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668336
Sequences flagged as poor quality0
Sequence length77
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163500.9800184135569814No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81480.48839082774692866No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC2200.025.8109324
ATTCGCG2450.023.18202856
CGCGCTT2650.022.77128838
TCGCGCT2700.022.34825737
CGATCGT2700.022.3449081
CGCGGAA2400.022.18991959
CGCTAGA800.002459467422.18326613
AATTCGC2750.021.94389755
GTTCGCG1003.5784225E-421.2972124
CCGCTAG850.003496180820.87836612
AACTCGA2750.020.65060213
TACGTAG2251.7644197E-1020.505352
ATACGTA2353.1468517E-1019.6321951
CTCGCGC3100.019.46227636
CGACTAA1501.3273753E-518.92915226
CAACGCG950.006654790618.67952510
AACGCGG950.006654790618.67952511
GCCTAGT3450.018.5220917
ACTCGAG3101.8189894E-1218.32073214
CAATTCG3101.8189894E-1218.3201854
Produced by FastQC (version 0.11.5)