##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936126_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1668336 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.71867237774645 40.0 36.0 41.0 30.0 41.0 2 36.57912195145342 39.0 36.0 41.0 30.0 41.0 3 36.434721782662486 39.0 36.0 41.0 30.0 41.0 4 36.32708399267294 39.0 36.0 41.0 30.0 41.0 5 36.23427415101035 39.0 35.0 41.0 30.0 41.0 6 36.11217404647505 39.0 35.0 40.0 29.0 41.0 7 35.92976235003021 39.0 35.0 40.0 29.0 41.0 8 35.76515941632861 38.0 35.0 40.0 29.0 41.0 9 35.87772127437159 39.0 35.0 40.0 29.0 41.0 10-11 36.0499764435941 39.0 35.0 41.0 30.0 41.0 12-13 35.927793921608114 39.0 35.0 41.0 29.0 41.0 14-15 35.73684947156927 39.0 35.0 41.0 27.5 41.0 16-17 35.52535910032512 39.0 35.0 41.0 27.0 41.0 18-19 35.28701652424931 39.0 35.0 41.0 26.0 41.0 20-21 34.97667706025645 39.0 34.5 41.0 24.5 41.0 22-23 34.56565643851118 38.5 34.0 41.0 23.5 41.0 24-25 34.23509592791859 38.0 34.0 40.0 21.5 41.0 26-27 33.222393450719764 37.5 32.0 39.5 17.5 40.5 28-29 33.203729644388176 37.5 33.0 39.5 17.5 40.5 30-31 33.57895801565152 38.0 33.0 40.0 17.5 41.0 32-33 33.43407143405165 38.0 33.0 40.0 14.5 41.0 34-35 33.16509564020677 37.0 33.0 40.0 12.5 41.0 36-37 32.84259315869225 37.0 32.5 40.0 8.0 41.0 38-39 32.51806710398864 36.0 32.0 40.0 7.5 41.0 40-41 32.13985851770866 36.0 32.0 39.0 4.0 41.0 42-43 31.765413861476347 35.0 31.0 39.0 2.0 41.0 44-45 31.404583668997134 35.0 31.0 39.0 2.0 40.0 46-47 30.983620206001667 35.0 31.0 38.0 2.0 40.0 48-49 30.581243826183695 35.0 30.5 37.0 2.0 39.5 50-51 30.14362394625543 35.0 30.0 37.0 2.0 39.0 52-53 29.6641632740647 34.5 29.5 36.0 2.0 39.0 54-55 29.215013642335837 34.0 29.0 36.0 2.0 37.5 56-57 28.7771519046523 34.0 29.0 35.0 2.0 37.0 58-59 28.35714927928187 34.0 28.5 35.0 2.0 37.0 60-61 28.008545041286645 34.0 27.5 35.0 2.0 36.0 62-63 27.634978205829043 34.0 27.0 35.0 2.0 36.0 64-65 27.254251841355696 34.0 26.0 35.0 2.0 35.5 66-67 26.89459077787688 33.0 25.5 35.0 2.0 35.0 68-69 26.5195098589253 33.0 25.0 35.0 2.0 35.0 70-71 26.064729766665707 33.0 24.0 35.0 2.0 35.0 72-73 25.583412154386167 33.0 21.5 35.0 2.0 35.0 74-75 25.020506360828996 33.0 14.0 35.0 2.0 35.0 76-77 23.33763072906177 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.996068958610195 1101 2 0.971479430583841 1101 3 1.0856761176211975 1101 4 1.0018343524130344 1101 5 1.1575384885619115 1101 6 1.1818364021956 1101 7 1.0454964908432984 1101 8 1.013188927725814 1101 9 1.131360587324039 1101 10-11 0.9985576074192721 1101 12-13 1.140616802618176 1101 14-15 1.2156681744831204 1101 16-17 1.2187501008342636 1101 18-19 1.3945252275263726 1101 20-21 1.3731418919345018 1101 22-23 1.1904075805118097 1101 24-25 1.2852998294146047 1101 26-27 1.2053066621219273 1101 28-29 1.2716727452579235 1101 30-31 1.2631517860289279 1101 32-33 1.243709720150946 1101 34-35 1.3766682347802188 1101 36-37 1.3748461263344893 1101 38-39 1.4088565902410721 1101 40-41 1.0777892376263836 1101 42-43 1.083202548523726 1101 44-45 1.0465197239627138 1101 46-47 1.0037917147206663 1101 48-49 1.0499398114222593 1101 50-51 0.9129970379318131 1101 52-53 1.025507355070527 1101 54-55 1.1278512629532074 1101 56-57 1.1325248329351432 1101 58-59 0.9756776823754549 1101 60-61 0.8635944312261756 1101 62-63 0.927575552316938 1101 64-65 0.7795321826211214 1101 66-67 0.7359946396613495 1101 68-69 0.7695603904644557 1101 70-71 0.7754037129129578 1101 72-73 0.6417991116603048 1101 74-75 0.8075879521124314 1101 76-77 0.9809728963471187 1102 1 0.8531897148571304 1102 2 0.8886475589580414 1102 3 0.9147950387316683 1102 4 0.824546300004954 1102 5 0.925121656519579 1102 6 0.8971608585897712 1102 7 0.8011406024025263 1102 8 0.9021898452940462 1102 9 0.7744958966298867 1102 10-11 0.9069554906106987 1102 12-13 1.087043087747169 1102 14-15 1.0758163724217908 1102 16-17 1.0139456709002062 1102 18-19 0.9523747036589327 1102 20-21 1.0310169746223394 1102 22-23 0.9278923766196172 1102 24-25 1.0197305103355845 1102 26-27 0.8512533578093553 1102 28-29 1.093861892346247 1102 30-31 1.2106876885533069 1102 32-33 1.0824391015392436 1102 34-35 1.0437189997760612 1102 36-37 1.1593056146301564 1102 38-39 1.241365712576389 1102 40-41 1.1984574620251678 1102 42-43 1.1694406254714274 1102 44-45 1.077447641509604 1102 46-47 1.1779029538645567 1102 48-49 1.059126163128294 1102 50-51 0.9727536689626923 1102 52-53 1.0596360920271906 1102 54-55 1.0364625341052864 1102 56-57 1.20653044503851 1102 58-59 1.212746371959991 1102 60-61 1.1189237955434415 1102 62-63 1.035547193056967 1102 64-65 1.0542398411150309 1102 66-67 1.0318757185508787 1102 68-69 1.2870982134804763 1102 70-71 1.25456637855536 1102 72-73 1.1463256037756224 1102 74-75 1.1433058959060425 1102 76-77 1.1403790376526253 1103 1 0.6114077823423685 1103 2 0.5226863686752878 1103 3 0.3656961306701305 1103 4 0.562520669853761 1103 5 0.35175475259347166 1103 6 0.2416601185378937 1103 7 0.3615940694050721 1103 8 0.37568934539709176 1103 9 0.45655504627706733 1103 10-11 0.5824438056258288 1103 12-13 0.6958905399759274 1103 14-15 0.6161491998968742 1103 16-17 0.5740533829391197 1103 18-19 0.515206726359537 1103 20-21 0.8083991070958874 1103 22-23 0.48897241804208136 1103 24-25 0.5872304379484632 1103 26-27 0.7721934195683531 1103 28-29 0.7053610552104601 1103 30-31 0.7221395443036585 1103 32-33 0.880228654289013 1103 34-35 0.9952066403135333 1103 36-37 0.8099945271371212 1103 38-39 0.634898306368818 1103 40-41 0.7044777287163555 1103 42-43 0.5536525255737921 1103 44-45 0.6660203122197323 1103 46-47 0.6317789436520087 1103 48-49 0.6070185481732828 1103 50-51 0.6995938347888142 1103 52-53 0.7085634426073959 1103 54-55 0.7949780275044347 1103 56-57 0.740402508889062 1103 58-59 0.6712085117914732 1103 60-61 0.7183819992186642 1103 62-63 0.5719240642640955 1103 64-65 0.5114843869582195 1103 66-67 0.5624260650722945 1103 68-69 0.5537481033668925 1103 70-71 0.37517460922672186 1103 72-73 0.36160895252315584 1103 74-75 0.3421599225887846 1103 76-77 0.5098742098573332 1104 1 0.5396466081271498 1104 2 0.40502170493788725 1104 3 0.548878707662368 1104 4 0.6097389429093241 1104 5 0.5955750635841497 1104 6 0.5592593939827708 1104 7 0.5691626817036806 1104 8 0.394661444741061 1104 9 0.29247790460408396 1104 10-11 0.4818888720296286 1104 12-13 0.5822245106799926 1104 14-15 0.6897341460588606 1104 16-17 0.649256524219112 1104 18-19 0.5782562728520944 1104 20-21 0.6019607545506105 1104 22-23 0.6422864981559471 1104 24-25 0.6444249664675041 1104 26-27 0.5239106878568407 1104 28-29 0.4300903790525794 1104 30-31 0.6595191084807794 1104 32-33 0.6333071466595612 1104 34-35 0.632183967418463 1104 36-37 0.6770296634370396 1104 38-39 0.6582127002143849 1104 40-41 0.8616930213207112 1104 42-43 0.7729949521729544 1104 44-45 0.6392476455167362 1104 46-47 0.6429081631370615 1104 48-49 0.49141563839426894 1104 50-51 0.4588864853759027 1104 52-53 0.3620368654037982 1104 54-55 0.47769041974171955 1104 56-57 0.5619581062755117 1104 58-59 0.3659991619261298 1104 60-61 0.31794805873191834 1104 62-63 0.3474849422505315 1104 64-65 0.2272477952734313 1104 66-67 0.34372080280084205 1104 68-69 0.2622164238950191 1104 70-71 0.33201173128321315 1104 72-73 0.25013037627066126 1104 74-75 0.22442528842472242 1104 76-77 0.07312933317865955 1105 1 -0.1849272310509491 1105 2 0.07230058917136972 1105 3 -0.14430941531683317 1105 4 0.054486307504014064 1105 5 -0.20650169319328882 1105 6 -0.018109601023830635 1105 7 -0.0018067265497734297 1105 8 -0.12866978409438445 1105 9 -0.10056577329294925 1105 10-11 0.21002122581484883 1105 12-13 0.2315529845363855 1105 14-15 0.1689354891111492 1105 16-17 0.021503936643306076 1105 18-19 -0.11743811579127339 1105 20-21 -0.228985886862084 1105 22-23 -0.23608548141128693 1105 24-25 -0.033979058863003786 1105 26-27 -0.2576902610803842 1105 28-29 -0.0785524111289746 1105 30-31 0.09733837037597226 1105 32-33 -0.005783423276739086 1105 34-35 -0.20606145529863085 1105 36-37 -0.007960483686495934 1105 38-39 -0.12052326182811868 1105 40-41 -0.1296067931008409 1105 42-43 -0.05256369635676705 1105 44-45 -0.2551185179836182 1105 46-47 -0.31286629208496564 1105 48-49 -0.3122155770489208 1105 50-51 -0.19638719500825985 1105 52-53 -0.26723670018957435 1105 54-55 -0.4061030333566755 1105 56-57 -0.4118289376702293 1105 58-59 -0.3763074634158521 1105 60-61 -0.2273799559613039 1105 62-63 -0.2496663328838693 1105 64-65 -0.18889752987148967 1105 66-67 -0.23828047902968663 1105 68-69 -0.21629856586947227 1105 70-71 -0.17816160281538984 1105 72-73 -0.6351457912473677 1105 74-75 -0.8179851269266329 1105 76-77 -0.8023370044210623 1106 1 -0.10997939891554154 1106 2 -0.18322534018948033 1106 3 -0.19368947339153664 1106 4 -0.014634732982663934 1106 5 -0.26405747692574977 1106 6 -0.15904288136123768 1106 7 -0.5040894156513858 1106 8 -0.4050297368396798 1106 9 -0.26746705734007037 1106 10-11 -0.3591094753874984 1106 12-13 -0.03274349454678571 1106 14-15 -0.0020743592984189263 1106 16-17 -0.14317538280068476 1106 18-19 -0.01885879303503657 1106 20-21 0.040752335345423774 1106 22-23 0.1391270543333647 1106 24-25 -0.03688555417617323 1106 26-27 0.08038234333814387 1106 28-29 0.2721950522431129 1106 30-31 0.38168221456795237 1106 32-33 0.3834826935571982 1106 34-35 0.20974126736705756 1106 36-37 0.2650789430742364 1106 38-39 0.37019969092821725 1106 40-41 0.3887554671754003 1106 42-43 0.35846387256553314 1106 44-45 0.2748297005804119 1106 46-47 0.10106915225275159 1106 48-49 0.2607380073526109 1106 50-51 0.29544812015188526 1106 52-53 0.316320209706312 1106 54-55 0.10032824307228339 1106 56-57 0.18068345564786625 1106 58-59 0.11325206315387604 1106 60-61 -0.004026969972102279 1106 62-63 -0.03655922590938587 1106 64-65 0.03446787703785148 1106 66-67 -0.05918875191454376 1106 68-69 0.10163144638583788 1106 70-71 -0.022273534627299085 1106 72-73 -0.19858771102478556 1106 74-75 -0.501153676052752 1106 76-77 -0.5287993607524193 1107 1 -0.22389325926015857 1107 2 -0.24830475630911764 1107 3 -0.01923852641574797 1107 4 -0.1819579052175655 1107 5 -0.15889179252921792 1107 6 -0.318747143785842 1107 7 -0.4447068600953159 1107 8 -0.24263169617385216 1107 9 -0.31329650523228736 1107 10-11 -0.22504682753019267 1107 12-13 -0.27636141825892224 1107 14-15 -0.5055724047109962 1107 16-17 -0.36119322597126313 1107 18-19 -0.3785470926128838 1107 20-21 -0.48232909890678854 1107 22-23 -0.5874245956201705 1107 24-25 -0.49681498078459185 1107 26-27 -0.6365548495588769 1107 28-29 -0.6400931242255936 1107 30-31 -0.5538150606468406 1107 32-33 -0.4904554134711958 1107 34-35 -0.5782899279400908 1107 36-37 -0.42986378207846343 1107 38-39 -0.26945626952986146 1107 40-41 -0.26868624355178383 1107 42-43 -0.3095931312578877 1107 44-45 -0.24227619319353977 1107 46-47 -0.40803349842197534 1107 48-49 -0.3530407238575499 1107 50-51 -0.48238503632688534 1107 52-53 -0.6534508669934418 1107 54-55 -0.5050364984061595 1107 56-57 -0.31364552292899717 1107 58-59 -0.3859254865759425 1107 60-61 -0.4621800035254715 1107 62-63 -0.4968948055253293 1107 64-65 -0.7438615823384218 1107 66-67 -0.6259398647201913 1107 68-69 -0.8184586192876893 1107 70-71 -0.8800989278798745 1107 72-73 -0.8290680970117776 1107 74-75 -0.9830327825620735 1107 76-77 -0.9215378349645711 1108 1 0.03714900355699058 1108 2 0.1952384364665818 1108 3 0.3483835007925933 1108 4 0.277298692422292 1108 5 0.3035494608667548 1108 6 0.3216671907473909 1108 7 0.41077178993775476 1108 8 0.22524854832202124 1108 9 0.23652285845894738 1108 10-11 0.16771121968709934 1108 12-13 0.10269294776987437 1108 14-15 0.057602538948714255 1108 16-17 0.06105626779837081 1108 18-19 0.036372011847873864 1108 20-21 0.1058324200592935 1108 22-23 0.050386759571900086 1108 24-25 0.15617695281998323 1108 26-27 0.06598813479031662 1108 28-29 0.18513364815963484 1108 30-31 0.31142001261363816 1108 32-33 0.20954520167649093 1108 34-35 0.33822385643400565 1108 36-37 0.34794414999765166 1108 38-39 0.3485006513158311 1108 40-41 0.3580622232830706 1108 42-43 0.23103122193240822 1108 44-45 0.11235355303270822 1108 46-47 0.04485523511197087 1108 48-49 0.09472961907643196 1108 50-51 0.07320260340209828 1108 52-53 0.11989561602630872 1108 54-55 0.23695464057234972 1108 56-57 0.2395956902290841 1108 58-59 0.12429953444763342 1108 60-61 0.2010919525428534 1108 62-63 0.17182500700660697 1108 64-65 0.2092201990134832 1108 66-67 0.406189298925014 1108 68-69 0.282110790358697 1108 70-71 0.0809510061907659 1108 72-73 0.1945289682271678 1108 74-75 0.1272141666672546 1108 76-77 0.36696172574760766 1109 1 -0.2777879288948455 1109 2 -0.21915944389000686 1109 3 -0.15303690780692847 1109 4 -0.1348922808107389 1109 5 -0.24223375384136148 1109 6 -0.43684260374339345 1109 7 -0.2478206976477324 1109 8 -0.04339009551122075 1109 9 -0.13925291668333983 1109 10-11 -0.2577705941606183 1109 12-13 -0.28678185966631276 1109 14-15 -0.20593564261887565 1109 16-17 -0.028619154773188882 1109 18-19 -0.13813924805455713 1109 20-21 0.030179313372549643 1109 22-23 -0.11416054495667538 1109 24-25 -0.31518739886193003 1109 26-27 -0.35710324389202697 1109 28-29 -0.25931237842309685 1109 30-31 -0.0808486752522839 1109 32-33 -0.06760464299269131 1109 34-35 -0.04257872364680537 1109 36-37 0.21797447783077217 1109 38-39 -0.026664945825352504 1109 40-41 0.046638341011039586 1109 42-43 0.2470150377124476 1109 44-45 0.025747654539888032 1109 46-47 -0.03680823915121323 1109 48-49 0.03811948045298763 1109 50-51 -0.08755794102223646 1109 52-53 -0.0059318423001677445 1109 54-55 -0.10072078340555635 1109 56-57 0.015669329273723775 1109 58-59 0.0012787894503567543 1109 60-61 0.05156652371305981 1109 62-63 0.005423963779776386 1109 64-65 -0.13465509754955107 1109 66-67 -0.02322377699090339 1109 68-69 -0.1376355963890319 1109 70-71 -0.102198201404061 1109 72-73 -0.14319339252005392 1109 74-75 -0.15940161140022724 1109 76-77 0.10786847381352871 1110 1 -0.22768326487498314 1110 2 -0.22603262454794049 1110 3 -0.4385254438778503 1110 4 -0.3108648863143273 1110 5 -0.4807147384397581 1110 6 -0.36226202306714583 1110 7 -0.35758352114716274 1110 8 -0.6522798339241831 1110 9 -0.5975469263440232 1110 10-11 -0.41763526099453685 1110 12-13 -0.4596386033609221 1110 14-15 -0.4376330448008261 1110 16-17 -0.4166162093139576 1110 18-19 -0.5719075642580265 1110 20-21 -0.4925293609318686 1110 22-23 -0.5643804256781877 1110 24-25 -0.6444380389915878 1110 26-27 -0.7378790120144316 1110 28-29 -0.5422548311318209 1110 30-31 -0.5024160338828523 1110 32-33 -0.43175711663421623 1110 34-35 -0.4208643243466028 1110 36-37 -0.38503877739944414 1110 38-39 -0.5888695205124534 1110 40-41 -0.5192243307687043 1110 42-43 -0.6149459293114141 1110 44-45 -0.6686284297647553 1110 46-47 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2314 10-11 -0.6183492052132138 2314 12-13 -0.5111943541072534 2314 14-15 -0.5448204353534791 2314 16-17 -0.7151024307603606 2314 18-19 -0.6718892187963093 2314 20-21 -0.6240602063241028 2314 22-23 -0.692923740425158 2314 24-25 -0.629508724611533 2314 26-27 -0.5801926804952586 2314 28-29 -0.8008432868330928 2314 30-31 -0.9023564111322528 2314 32-33 -1.0439797805064401 2314 34-35 -1.0314336729715237 2314 36-37 -1.4157392126872175 2314 38-39 -1.1043789406290863 2314 40-41 -0.9365598192305207 2314 42-43 -0.823396099145917 2314 44-45 -0.9977771447485502 2314 46-47 -0.8859363434510037 2314 48-49 -0.6658192644853322 2314 50-51 -0.6548495638015304 2314 52-53 -0.661635444575289 2314 54-55 -0.86883163704411 2314 56-57 -0.7488819662813633 2314 58-59 -0.7450399381670856 2314 60-61 -0.6127015520306855 2314 62-63 -0.7147250192036303 2314 64-65 -0.5939410793392206 2314 66-67 -0.477640016487193 2314 68-69 -0.39030102250611165 2314 70-71 -0.29088462323773356 2314 72-73 -0.43047073727580454 2314 74-75 -0.27037228293546534 2314 76-77 -0.349590037995096 2315 1 -1.0266853127171132 2315 2 -0.7578809921094631 2315 3 -0.8607677730884689 2315 4 -0.7461583590188567 2315 5 -0.8292387068396323 2315 6 -0.6884880509231124 2315 7 -1.1466961897649313 2315 8 -0.9428072288638347 2315 9 -0.6393823037988824 2315 10-11 -0.8997756304425479 2315 12-13 -0.704341892313515 2315 14-15 -0.7869745111032742 2315 16-17 -0.704997142425789 2315 18-19 -0.6427263931025635 2315 20-21 -0.7567080609434882 2315 22-23 -0.7670491197471918 2315 24-25 -0.9157604499350427 2315 26-27 -0.8258958152274545 2315 28-29 -0.8388549667127023 2315 30-31 -0.9053250887247231 2315 32-33 -0.7845704764658166 2315 34-35 -0.7857088626948254 2315 36-37 -0.8120529998283885 2315 38-39 -0.8402140968674061 2315 40-41 -0.9918868957227716 2315 42-43 -1.0805324607438287 2315 44-45 -0.9638177951145437 2315 46-47 -0.9987658989279389 2315 48-49 -0.8058520020318589 2315 50-51 -0.7170061808220645 2315 52-53 -0.7437141292932843 2315 54-55 -1.0205430084832194 2315 56-57 -1.1612974441150037 2315 58-59 -1.0279966070096123 2315 60-61 -0.9915964231483976 2315 62-63 -1.0731067280337996 2315 64-65 -0.7464289086455516 2315 66-67 -0.9041450965372206 2315 68-69 -0.9650654566747434 2315 70-71 -0.7813754123770416 2315 72-73 -0.7190194474563363 2315 74-75 -0.5737233968591404 2315 76-77 -0.5604550915361806 2316 1 -1.8991433268459872 2316 2 -1.6207146286116512 2316 3 -1.7537414922411756 2316 4 -2.699637564266915 2316 5 -1.947594621592721 2316 6 -1.8989596018428685 2316 7 -1.6056406577830842 2316 8 -1.3687389111604134 2316 9 -1.6733580480976045 2316 10-11 -1.5749784465687213 2316 12-13 -2.221636770897952 2316 14-15 -3.3032621618790685 2316 16-17 -2.5535345582868345 2316 18-19 -2.094734297421823 2316 20-21 -1.785620529023042 2316 22-23 -2.0830394676841664 2316 24-25 -2.095532042629607 2316 26-27 -2.09688792845143 2316 28-29 -2.730984144056066 2316 30-31 -2.659459670477794 2316 32-33 -2.5607963641255864 2316 34-35 -2.6487183426149628 2316 36-37 -2.538213813088433 2316 38-39 -2.8878050283358974 2316 40-41 -2.395256620283174 2316 42-43 -2.6684580072975166 2316 44-45 -2.7471861052524424 2316 46-47 -2.561381780151539 2316 48-49 -2.2474458094927776 2316 50-51 -2.4885452863149595 2316 52-53 -2.6325310635489494 2316 54-55 -2.666578568600009 2316 56-57 -2.700190773632688 2316 58-59 -2.6848914233156016 2316 60-61 -2.579534853007843 2316 62-63 -2.481538906474711 2316 64-65 -2.6119936689119037 2316 66-67 -2.259555070737715 2316 68-69 -2.2978648181013597 2316 70-71 -2.2592753348579144 2316 72-73 -2.168392715748162 2316 74-75 -2.504908726775927 2316 76-77 -2.859979067094084 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38516.0 3 4182.0 4 6021.0 5 7644.0 6 8314.0 7 8435.0 8 10491.0 9 11904.0 10 15591.0 11 17726.0 12 12616.0 13 9230.0 14 8827.0 15 8977.0 16 9082.0 17 9260.0 18 9780.0 19 10241.0 20 11136.0 21 11833.0 22 13163.0 23 15022.0 24 17357.0 25 19876.0 26 23433.0 27 27825.0 28 32178.0 29 37853.0 30 44768.0 31 53450.0 32 65515.0 33 82531.0 34 111825.0 35 148638.0 36 202012.0 37 261656.0 38 224523.0 39 56562.0 40 343.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.036275663392438 26.12545488439897 30.332779669612016 21.505489782596577 2 21.83422081312451 26.597958167062004 30.06209661031275 21.50572440950074 3 21.92027258792477 26.627797470145232 29.620677645236455 21.831252296693542 4 21.74810480595431 26.549083202690593 30.1659737355631 21.536838255791995 5 21.946349119187012 26.623176537870823 29.860177757209023 21.570296585733143 6 21.76945091546062 26.67826453940419 30.018068222827825 21.534216322307362 7 21.93150630730439 26.45003255266878 29.651284781297623 21.96717635872921 8 21.55427566993659 26.802739277627335 29.57169261242747 22.07129244000861 9 21.680637104732124 26.693088799709358 29.710991183696372 21.915282911862143 10-11 22.18335972239625 26.604252186315374 29.53607607724032 21.676312014048055 12-13 22.194108030040784 26.36545345650687 29.28134249480965 22.159096018642696 14-15 22.044513074370588 26.562718704189198 29.514751321254295 21.87801690018592 16-17 22.04143900589545 26.599999400564066 29.547006267102656 21.811555326437823 18-19 22.259448889464373 26.598663556651374 29.031375306550515 22.110512247333737 20-21 21.977176807060907 26.810223049608506 29.01904870092793 22.193551442402654 22-23 22.270913301437012 26.984532822416707 28.44621437516523 22.298339500981047 24-25 22.339849285079858 27.25632477779307 27.915024339269547 22.48880159785753 26-27 22.29101211361512 27.370493662637585 27.775379873066914 22.563114350680376 28-29 22.63925754883695 27.26270288286085 27.71240807479181 22.385631493510385 30-31 22.184873496218405 27.19443163964271 27.899048741436182 22.721646122702705 32-33 22.28528798912353 27.547045057271724 27.631567904816485 22.536099048788266 34-35 22.347682841734986 27.035381413338257 27.759350999062445 22.857584745864315 36-37 22.172903877083325 27.36551717523275 28.048923771735424 22.4126551759485 38-39 22.302767905236394 27.29162776184283 28.09033692601584 22.31526740690494 40-41 22.087880698012423 27.297076708051172 27.950770215746733 22.664272378189672 42-43 22.008459904178636 27.500383669123746 27.78924455549534 22.701911871202274 44-45 22.167814161835935 27.131743676087126 28.118642477594392 22.581799684482544 46-47 21.983467804422872 27.267751487714758 28.077875231891063 22.670905475971303 48-49 22.088578759321873 27.35270843824185 27.781814257967053 22.776898544469223 50-51 21.920757115192067 27.56221858493564 27.70578148701069 22.811242812861604 52-53 21.891588778322433 27.34560659973121 27.99423560798177 22.76856901396459 54-55 21.842383814922854 27.38152016434728 27.883579151781714 22.892516868948153 56-57 21.88531398269941 27.658971154794383 27.781296432124456 22.674418430381753 58-59 21.92096417495814 27.39051927396724 27.72041971633238 22.968096834742237 60-61 21.681638491578646 27.232464559819952 27.805508560438653 23.280388388162745 62-63 21.93795939901915 27.065362063383574 27.832685828027387 23.16399270956989 64-65 21.814368353244248 27.05359610506633 27.750712974059553 23.38132256762987 66-67 21.641765990598476 27.10522671395607 27.977874458866676 23.275132836578784 68-69 21.929917915698486 26.91031643271896 27.9336646364185 23.226101015164055 70-71 21.613757153189503 26.652325303849377 28.213582753901495 23.520334789059625 72-73 21.922914634259715 26.39304533708241 28.046211273557052 23.637828755100823 74-75 21.75057203776814 26.46586083998449 27.932478998288314 23.851088123959062 76-77 21.609382320002542 26.221782468044672 28.52240396906471 23.64643124288807 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 18841.0 1 10958.0 2 2868.5 3 2679.5 4 2697.0 5 2694.0 6 2640.0 7 2738.0 8 2847.0 9 2807.0 10 2821.0 11 2939.5 12 3166.0 13 3288.0 14 3434.5 15 3697.0 16 4083.0 17 4353.0 18 4673.0 19 5360.0 20 6107.5 21 7175.5 22 7863.0 23 8716.0 24 10697.0 25 13231.0 26 14637.0 27 15739.5 28 19528.0 29 23580.5 30 24947.0 31 28165.0 32 33958.5 33 39015.0 34 43979.0 35 46462.0 36 49568.0 37 55175.0 38 57923.5 39 58171.0 40 60657.5 41 64313.5 42 68439.5 43 71396.0 44 69947.5 45 68635.5 46 70440.5 47 73472.0 48 74835.0 49 74572.0 50 74228.5 51 72311.5 52 70475.0 53 69612.5 54 65770.0 55 59470.5 56 53277.0 57 50403.0 58 49108.0 59 43499.0 60 36975.5 61 34766.0 62 30692.5 63 22277.5 64 15753.5 65 13571.0 66 11904.5 67 9111.5 68 6796.0 69 4847.0 70 4087.0 71 3457.5 72 2517.0 73 1982.0 74 1758.0 75 1562.5 76 1084.0 77 624.0 78 447.0 79 370.0 80 258.0 81 195.0 82 131.5 83 96.0 84 92.0 85 72.0 86 47.0 87 38.0 88 38.5 89 33.0 90 27.5 91 28.0 92 25.5 93 18.5 94 12.5 95 13.5 96 16.0 97 17.5 98 18.0 99 28.0 100 39.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0032966980272559005 2 0.007732255373018385 3 0.010609373651350807 4 0.018041929203709565 5 0.013066912180759751 6 0.012827152324232049 7 0.016183790315619874 8 0.018281689060237267 9 0.017382589598258384 10-11 0.020799167553778135 12-13 0.020199767912458883 14-15 0.007762225355084347 16-17 0.006053936377324472 18-19 0.010609373651350807 20-21 0.009860124099701738 22-23 0.013366612001419378 24-25 0.021128837356503727 26-27 0.012767212360100123 28-29 0.00791207526541416 30-31 0.007492495516490684 32-33 0.008871114691524969 34-35 0.01030967383069118 36-37 0.008931054655656894 38-39 0.016153820333553913 40-41 0.01195802284431913 42-43 0.013906071678606706 44-45 0.016933039867268945 46-47 0.015434540763970809 48-49 0.012947032252495898 50-51 0.025534424720200247 52-53 0.030599351689347947 54-55 0.02424571549136385 56-57 0.014535441301991926 58-59 0.015404570781904844 60-61 0.018821148737424593 62-63 0.02373622579624248 64-65 0.019360608414611926 66-67 0.018521448916764965 68-69 0.02862133287299441 70-71 0.053826087790469074 72-73 0.06476513124454547 74-75 0.07609378446547937 76-77 0.07450537541598336 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1668336.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.296714669829925 #Duplication Level Percentage of deduplicated Percentage of total 1 74.71049179158736 36.82981796692553 2 12.09164529424935 11.921567759188038 3 4.939431831050911 7.30493284819177 4 2.538975689919985 5.006526405584803 5 1.4616377722023817 3.602697010345334 6 0.9542350544964489 2.822439192567663 7 0.6464466228292949 2.230738630042364 8 0.4601507768402311 1.8147137240794766 9 0.3322010223036902 1.4738777108577743 >10 1.670140054548928 14.858232081803349 >50 0.12876494651911352 4.391895601186422 >100 0.06294590727867873 5.323059862643982 >500 0.002566581651976541 0.8809688391519694 >1k 1.2221817390364482E-4 0.06253902715358667 >5k 1.2221817390364482E-4 0.4909132882923162 >10k+ 1.2221817390364482E-4 0.9850800519856859 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16350 0.9800184135569814 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8148 0.48839082774692866 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.993996413192546E-5 0.0 2 0.0 0.0 0.0 8.391594978469565E-4 0.0 3 0.0 0.0 0.0 0.0016183790315619875 0.0 4 0.0 0.0 0.0 0.0030569381707281984 0.0 5 0.0 0.0 0.0 0.006413576162116024 0.0 6 0.0 0.0 0.0 0.013006972216627825 0.0 7 0.0 0.0 0.0 0.02355640590384671 0.0 8 0.0 0.0 0.0 0.03536457883783602 0.0 9 0.0 0.0 0.0 0.048791130803387324 0.0 10 0.0 0.0 0.0 0.06455534137008373 0.0 11 0.0 0.0 0.0 0.08187799100421018 0.0 12 0.0 0.0 0.0 0.10669313615482733 0.0 13 0.0 0.0 0.0 0.1386411370371436 0.0 14 0.0 0.0 0.0 0.1812584515349426 0.0 15 0.0 0.0 0.0 0.22429534578166507 0.0 16 0.0 0.0 0.0 0.2711683977328308 0.0 17 0.0 0.0 0.0 0.3163631306883026 0.0 18 0.0 0.0 0.0 0.36005936454047627 0.0 19 0.0 0.0 0.0 0.40327607867959453 0.0 20 0.0 0.0 0.0 0.4553039675461058 0.0 21 0.0 0.0 0.0 0.5109282542605327 0.0 22 0.0 0.0 0.0 0.5706883985000624 0.0 23 0.0 0.0 0.0 0.6325464414842095 0.0 24 0.0 0.0 0.0 0.6974014826749527 0.0 25 0.0 0.0 0.0 0.7659728016418755 0.0 26 0.0 0.0 0.0 0.8388597980262968 0.0 27 0.0 0.0 0.0 0.9099485954867604 0.0 28 0.0 0.0 0.0 0.9809175130189602 0.0 29 0.0 0.0 0.0 1.0507475712326533 0.0 30 0.0 0.0 0.0 1.1216565488007213 0.0 31 0.0 0.0 0.0 1.206291778155 0.0 32 0.0 0.0 0.0 1.2962616643170202 0.0 33 0.0 0.0 0.0 1.3892285486856364 0.0 34 0.0 0.0 0.0 1.4884291893239732 0.0 35 0.0 0.0 0.0 1.5948226256581408 0.0 36 0.0 0.0 0.0 1.7095477170066462 0.0 37 0.0 0.0 0.0 1.8224746094311937 0.0 38 0.0 0.0 0.0 1.935581321748137 0.0 39 0.0 0.0 0.0 2.049826893383587 0.0 40 0.0 0.0 0.0 2.1683282024724035 0.0 41 0.0 0.0 0.0 2.2901262095884762 0.0 42 0.0 0.0 0.0 2.425590528526628 0.0 43 0.0 0.0 0.0 2.5784973770271695 0.0 44 0.0 0.0 0.0 2.733981643985384 0.0 45 0.0 0.0 0.0 2.892462909150195 0.0 46 0.0 0.0 0.0 3.071443642048125 0.0 47 0.0 0.0 0.0 3.2456291778155 0.0 48 0.0 0.0 0.0 3.4294650478081152 0.0 49 0.0 0.0 0.0 3.609284940203892 0.0 50 0.0 0.0 0.0 3.790183751954043 0.0 51 0.0 0.0 0.0 3.9801934382522464 0.0 52 0.0 0.0 0.0 4.1822510573409675 0.0 53 0.0 0.0 0.0 4.397015948825656 0.0 54 0.0 0.0 0.0 4.628683910195548 0.0 55 0.0 0.0 0.0 4.871500704893978 0.0 56 0.0 0.0 0.0 5.129782010338445 0.0 57 0.0 0.0 0.0 5.396035331012458 0.0 58 0.0 0.0 0.0 5.675715203652022 0.0 59 0.0 0.0 0.0 5.945325162317423 0.0 60 0.0 0.0 0.0 6.223626415781953 0.0 61 0.0 0.0 0.0 6.5094201647629735 0.0 62 0.0 0.0 0.0 6.802886229152881 0.0 63 0.0 0.0 0.0 7.109119505902887 0.0 64 0.0 0.0 0.0 7.431296813111987 0.0 65 0.0 0.0 0.0 7.772175389130247 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 220 0.0 25.810932 4 ATTCGCG 245 0.0 23.182028 56 CGCGCTT 265 0.0 22.771288 38 TCGCGCT 270 0.0 22.348257 37 CGATCGT 270 0.0 22.344908 1 CGCGGAA 240 0.0 22.189919 59 CGCTAGA 80 0.0024594674 22.183266 13 AATTCGC 275 0.0 21.943897 55 GTTCGCG 100 3.5784225E-4 21.29721 24 CCGCTAG 85 0.0034961808 20.878366 12 AACTCGA 275 0.0 20.650602 13 TACGTAG 225 1.7644197E-10 20.50535 2 ATACGTA 235 3.1468517E-10 19.632195 1 CTCGCGC 310 0.0 19.462276 36 CGACTAA 150 1.3273753E-5 18.929152 26 CAACGCG 95 0.0066547906 18.679525 10 AACGCGG 95 0.0066547906 18.679525 11 GCCTAGT 345 0.0 18.52209 17 ACTCGAG 310 1.8189894E-12 18.320732 14 CAATTCG 310 1.8189894E-12 18.32018 54 >>END_MODULE