FastQCFastQC Report
Thu 26 May 2016
SRR936126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668336
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112300.673125797201523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83850.5025965992461949No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62450.3743250760038745No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19630.11766214959096968No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18890.11322659224520719No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA11550.051.022377100-101
CGTACAT13250.048.968643100-101
CCCGGTA2850.045.94790698-99
ACGTATC3450.043.13486100-101
CGGTATC11000.042.479992100-101
CCGGTAT3900.041.973537100-101
GGTATCA84300.041.861751
TACGCAG7900.041.442223100-101
AGGTATC17050.040.324135100-101
CGAGTAC10800.039.408485100-101
TCGAGTA4050.038.94918100-101
TCGTATC4600.038.174347100-101
TCGCATA801.6916601E-1037.20382100-101
CTACGCG401.1952926E-437.20158896-97
AGTATCA26950.036.665432100-101
CCGTACA6300.034.959778100-101
CCGTATC5150.034.6754100-101
ACGTACA4550.034.66905100-101
TCGTACA7800.034.341984100-101
ACCGTAT1750.034.0133998-99
Produced by FastQC (version 0.11.5)