FastQCFastQC Report
Thu 26 May 2016
SRR936125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3266793
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88000.2693773373458312No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG500.00673165428.39832956
ATTCGCG4700.024.16878956
CGCGGAA4900.022.45958159
TCGCGCT5200.021.84419837
CTCGCGC5550.021.10525136
ATGCGCG850.00349607420.87920627
AATTCGC5350.020.5685755
CCTCGCG6000.020.1127135
CAATTCG5650.020.10716654
CGGTTAC3300.019.3586434
CGCGCTT6100.018.62042838
CGATCGT5800.017.746511
GACGTAT4450.017.54818339
TCGCGGA6350.017.32968758
CGCTCCG6500.016.92925525
CGCGTTA1553.6413738E-416.03303354
AACTCGA6900.015.94760413
ACTCGAG7350.015.45368914
CGGCCAA7150.015.39494450
ACGCTCC7650.014.84948124
Produced by FastQC (version 0.11.5)