##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936125_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3266793 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.67237685399718 40.0 36.0 41.0 30.0 41.0 2 36.53839744360907 39.0 36.0 41.0 30.0 41.0 3 36.45123030446067 39.0 36.0 41.0 30.0 41.0 4 36.327941194927256 39.0 36.0 41.0 29.0 41.0 5 36.257986655414044 39.0 36.0 41.0 29.0 41.0 6 36.15382731627012 39.0 35.0 41.0 29.0 41.0 7 35.99004681349568 39.0 35.0 40.0 29.0 41.0 8 35.81750022116491 39.0 35.0 40.0 28.0 41.0 9 35.72385425094274 39.0 35.0 40.0 27.0 41.0 10-11 35.93496282133579 39.0 35.0 40.0 29.0 41.0 12-13 36.12320248023061 39.0 35.0 41.0 29.0 41.0 14-15 36.07238643525929 39.0 35.0 41.0 29.0 41.0 16-17 35.90823875280742 39.0 35.0 41.0 28.0 41.0 18-19 35.761803089451945 39.0 35.0 41.0 27.5 41.0 20-21 35.56084361635402 39.0 35.0 41.0 27.0 41.0 22-23 35.304271345016346 39.0 35.0 41.0 26.0 41.0 24-25 35.07908719652577 39.0 34.5 41.0 25.5 41.0 26-27 34.12937428236194 38.0 33.0 40.0 23.5 40.5 28-29 34.159302104541055 38.0 33.5 39.5 24.0 40.5 30-31 34.530452189655115 38.0 34.0 40.0 24.0 41.0 32-33 34.368074591809155 38.0 34.0 40.0 23.0 41.0 34-35 34.0903523731072 38.0 33.5 40.0 22.5 41.0 36-37 33.796160944387964 37.5 33.0 40.0 22.0 41.0 38-39 33.51353651731224 37.0 33.0 40.0 21.5 41.0 40-41 33.13172490574089 36.5 32.5 40.0 20.0 41.0 42-43 32.77274363573082 36.0 32.0 39.0 19.0 41.0 44-45 32.40666488510291 35.5 32.0 39.0 18.0 40.5 46-47 32.019015131965816 35.0 31.5 38.5 16.0 40.0 48-49 31.559088072002112 35.0 31.0 37.5 12.0 40.0 50-51 31.12548897343664 35.0 31.0 37.0 7.5 39.0 52-53 30.677482472871713 35.0 30.5 36.5 6.5 39.0 54-55 30.228327904461654 34.5 30.0 36.0 2.0 38.5 56-57 29.78805590069527 34.0 30.0 35.5 2.0 37.0 58-59 29.369964059553208 34.0 29.0 35.0 2.0 37.0 60-61 29.027222875768377 34.0 29.0 35.0 2.0 36.0 62-63 28.695614169615276 34.0 29.0 35.0 2.0 36.0 64-65 28.335526615858427 34.0 29.0 35.0 2.0 36.0 66-67 27.963897467638752 34.0 27.5 35.0 2.0 35.0 68-69 27.61588567136026 34.0 27.0 35.0 2.0 35.0 70-71 27.203162551162563 33.5 26.5 35.0 2.0 35.0 72-73 26.728076587650335 33.0 25.5 35.0 2.0 35.0 74-75 26.155410673403548 33.0 24.5 35.0 2.0 35.0 76-77 24.446347840221282 31.0 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8687841773314773 1101 2 0.8561217966582149 1101 3 0.9311799675729091 1101 4 0.9007239776526319 1101 5 0.973587850416358 1101 6 0.8747131738322693 1101 7 0.9864462432656467 1101 8 1.1477126952036016 1101 9 1.0140199771633789 1101 10-11 0.9426537586359061 1101 12-13 1.0073648883383655 1101 14-15 1.0699232423287626 1101 16-17 1.0743287635402936 1101 18-19 1.1346994157454589 1101 20-21 1.059830302268935 1101 22-23 1.0423464829554803 1101 24-25 1.1602865711219223 1101 26-27 1.0586906192048318 1101 28-29 1.1001642345807312 1101 30-31 1.0794242375304464 1101 32-33 1.1835225551842044 1101 34-35 1.2019439062971742 1101 36-37 1.197748224485288 1101 38-39 1.1572971993195864 1101 40-41 1.1702982015282188 1101 42-43 1.1395730420027022 1101 44-45 1.0503325833058952 1101 46-47 1.027738085411002 1101 48-49 1.2263370245845842 1101 50-51 1.2099688315459005 1101 52-53 1.2529956327454528 1101 54-55 1.21030142903437 1101 56-57 1.153913765725136 1101 58-59 1.1681951590133899 1101 60-61 1.1651065219142396 1101 62-63 1.1903329046738982 1101 64-65 1.007392246393696 1101 66-67 0.9408287731657516 1101 68-69 1.0425341082770068 1101 70-71 0.98437507400158 1101 72-73 0.9967400068599659 1101 74-75 0.9199919004592338 1101 76-77 1.0255676953529225 1102 1 0.9480882677055149 1102 2 1.0467831759901642 1102 3 1.0184216617280484 1102 4 1.062965895594509 1102 5 1.1795521027224822 1102 6 0.9799466505383094 1102 7 0.8464442515636605 1102 8 0.9713659466011535 1102 9 1.108657823081728 1102 10-11 0.9514676553448993 1102 12-13 1.042597704873522 1102 14-15 0.9597039648913892 1102 16-17 1.0525397675818766 1102 18-19 1.008337337751307 1102 20-21 1.0446611418720266 1102 22-23 1.0848086182390375 1102 24-25 1.0431736550464308 1102 26-27 1.0664683273600772 1102 28-29 1.0781477594009203 1102 30-31 1.2255761685862296 1102 32-33 1.2024979923516028 1102 34-35 1.2278795586578966 1102 36-37 1.27306146997487 1102 38-39 1.3965473235212187 1102 40-41 1.3691912404178836 1102 42-43 1.2694406273885264 1102 44-45 1.2855413427664786 1102 46-47 1.1521443791892807 1102 48-49 1.2040772932181874 1102 50-51 1.27403517088716 1102 52-53 1.2648190135364956 1102 54-55 1.1788177677042704 1102 56-57 1.1793324707160266 1102 58-59 1.1161517622887338 1102 60-61 1.056600555759747 1102 62-63 1.0758897644088457 1102 64-65 1.0271818756685782 1102 66-67 1.0811060225581706 1102 68-69 1.1330078424273111 1102 70-71 1.2284776779840882 1102 72-73 1.15505340452453 1102 74-75 1.0396037311690414 1102 76-77 1.1506994497962069 1103 1 0.6948185166903471 1103 2 0.5647752089163305 1103 3 0.634908986587412 1103 4 0.7855745337538167 1103 5 0.6771790607438248 1103 6 0.7402402091095226 1103 7 0.823647731510988 1103 8 0.8346226395492451 1103 9 0.7249787540062087 1103 10-11 0.799668176565639 1103 12-13 0.8736122670976911 1103 14-15 0.8996889233204541 1103 16-17 0.8863488663548651 1103 18-19 0.9795117924254839 1103 20-21 0.9454482072229737 1103 22-23 1.0650890085485258 1103 24-25 0.9544765966928495 1103 26-27 0.7975305487741977 1103 28-29 0.8696748920807948 1103 30-31 1.0426511064639854 1103 32-33 1.020028585143777 1103 34-35 1.0968330399826556 1103 36-37 1.0202476493607477 1103 38-39 0.9935255104884959 1103 40-41 1.0714681375566784 1103 42-43 1.073202122068885 1103 44-45 0.9618150552796365 1103 46-47 0.8259965640738827 1103 48-49 1.0022979249641644 1103 50-51 0.9773831601101222 1103 52-53 0.9776553138610211 1103 54-55 1.1718074589939356 1103 56-57 1.1177882027666932 1103 58-59 0.9577535518499936 1103 60-61 0.9852875313020419 1103 62-63 0.8502689552494651 1103 64-65 0.7239964235674456 1103 66-67 0.7501146012123634 1103 68-69 0.668443689851788 1103 70-71 0.656127721786465 1103 72-73 0.731913628109492 1103 74-75 0.7740249612720156 1103 76-77 0.8339955924321742 1104 1 0.5149073560331203 1104 2 0.5488633866895256 1104 3 0.5796937026118911 1104 4 0.4845299841312638 1104 5 0.6073718717593906 1104 6 0.4071957754764455 1104 7 0.7266104033069638 1104 8 0.6897619933804933 1104 9 0.6364555899423223 1104 10-11 0.5680239398998665 1104 12-13 0.7009862654802888 1104 14-15 0.567305146381095 1104 16-17 0.5900828144323924 1104 18-19 0.6180753380088078 1104 20-21 0.5859843255923707 1104 22-23 0.5991312201240575 1104 24-25 0.5220434168307619 1104 26-27 0.4568708693991752 1104 28-29 0.49071828180863974 1104 30-31 0.6022484833835904 1104 32-33 0.6036479184405934 1104 34-35 0.7076616773533928 1104 36-37 0.5482088981377586 1104 38-39 0.6238480041762031 1104 40-41 0.7264746941837004 1104 42-43 0.676399779940752 1104 44-45 0.6325315265362619 1104 46-47 0.4441691607022449 1104 48-49 0.5944865173997123 1104 50-51 0.45505663686229525 1104 52-53 0.4906544579016838 1104 54-55 0.503881071496739 1104 56-57 0.5449140588602752 1104 58-59 0.5731613568673986 1104 60-61 0.43210034470183345 1104 62-63 0.490368663235234 1104 64-65 0.4248263389703091 1104 66-67 0.5235167657047661 1104 68-69 0.4076711140585765 1104 70-71 0.39081147327258137 1104 72-73 0.20872497016137004 1104 74-75 0.21035463556713907 1104 76-77 0.09720319532718591 1105 1 0.04655956970189834 1105 2 0.29467864847903513 1105 3 0.10022651443394182 1105 4 0.31944674910035076 1105 5 0.15715018285603577 1105 6 0.09228937887024813 1105 7 0.10576664652748491 1105 8 0.3175472992027082 1105 9 0.05715890561511827 1105 10-11 0.22672959364484058 1105 12-13 0.24130964230396756 1105 14-15 0.0909668987742549 1105 16-17 0.11890417266674547 1105 18-19 0.2339391980566745 1105 20-21 0.17377786636075854 1105 22-23 0.15754594955512857 1105 24-25 0.35738114496017914 1105 26-27 0.16557665025935364 1105 28-29 0.2765917263423461 1105 30-31 0.2686495852181139 1105 32-33 0.3906031132997114 1105 34-35 0.4478482782252371 1105 36-37 0.418127574071967 1105 38-39 0.38383788156936305 1105 40-41 0.531293387659808 1105 42-43 0.3407863707281251 1105 44-45 0.23151809003623214 1105 46-47 0.1982376944369264 1105 48-49 0.22687635937010597 1105 50-51 0.42905395218933506 1105 52-53 0.3696259074047461 1105 54-55 0.2923819871498772 1105 56-57 0.36193205074524215 1105 58-59 0.3508254371635253 1105 60-61 0.06881649943322898 1105 62-63 0.0638492976587095 1105 64-65 0.2509337559725573 1105 66-67 0.2253479448592266 1105 68-69 -0.06920069958716013 1105 70-71 -0.2484705017077573 1105 72-73 -0.2637778941677382 1105 74-75 -0.3743116594994369 1105 76-77 -0.15292942819488786 1106 1 0.028172811290254174 1106 2 0.06964164500906378 1106 3 -0.3292493864760786 1106 4 -0.05086726985622647 1106 5 -0.22929262336560896 1106 6 -0.030878801689809166 1106 7 0.06264937632874279 1106 8 0.09636148355678387 1106 9 -0.004894900890064946 1106 10-11 -0.11378713850572808 1106 12-13 -0.12657510095861824 1106 14-15 -0.020010389449453214 1106 16-17 0.09477828998419113 1106 18-19 0.19742105580807845 1106 20-21 -0.07737369259501037 1106 22-23 -0.1166337295062263 1106 24-25 0.015061073805497927 1106 26-27 -0.013438289522461844 1106 28-29 0.17586349214624875 1106 30-31 0.2576618330713245 1106 32-33 0.03224718548728589 1106 34-35 0.1193298262782676 1106 36-37 0.11461452285950458 1106 38-39 0.1531883335433264 1106 40-41 0.1748741294844507 1106 42-43 0.17398795567492442 1106 44-45 -0.01106127966429682 1106 46-47 0.17197831066072666 1106 48-49 0.031744868362327594 1106 50-51 0.0384795840752723 1106 52-53 -0.007272893800966074 1106 54-55 0.01811549550076208 1106 56-57 -0.11805759503408453 1106 58-59 -0.28613634588896986 1106 60-61 -0.2323184353866523 1106 62-63 -0.33854339649769827 1106 64-65 -0.23393121300697217 1106 66-67 -0.14136656893964883 1106 68-69 -0.24435327033677723 1106 70-71 -0.40735283236097786 1106 72-73 -0.2696047803742587 1106 74-75 -0.5193170560146108 1106 76-77 -0.2487798075964882 1107 1 -0.09464392038332647 1107 2 -0.031119742717471865 1107 3 -0.09952883394316814 1107 4 -0.07514035651046669 1107 5 -0.10985906489030839 1107 6 -0.35422391557462873 1107 7 -0.32486270859115507 1107 8 -0.15609755463368202 1107 9 -0.2863558329806253 1107 10-11 -0.17406578215817348 1107 12-13 0.029624264007672707 1107 14-15 -0.034560592709794946 1107 16-17 -0.08683561870850554 1107 18-19 0.02203429304358906 1107 20-21 0.01773656599356599 1107 22-23 -0.10876651968381168 1107 24-25 -0.2800020034829913 1107 26-27 -0.21448718703729952 1107 28-29 -0.1447383272952294 1107 30-31 0.06633843789529692 1107 32-33 0.08428340509266263 1107 34-35 0.1749087229006534 1107 36-37 0.1075235280210407 1107 38-39 0.0818206238046173 1107 40-41 0.17952542603342891 1107 42-43 0.11908943072514688 1107 44-45 0.11233005859843104 1107 46-47 0.15499007113363916 1107 48-49 0.19721278014256427 1107 50-51 0.1724192770261368 1107 52-53 0.1831188117184226 1107 54-55 0.14529448285951574 1107 56-57 0.13984255305717852 1107 58-59 0.03797208971248622 1107 60-61 0.016075496638833897 1107 62-63 0.09419791762036667 1107 64-65 -0.009269354051660628 1107 66-67 0.175318114725048 1107 68-69 0.09381673383942868 1107 70-71 0.11334448791442853 1107 72-73 0.2490255879843737 1107 74-75 0.1227449420671114 1107 76-77 0.30381444873496477 1108 1 -0.04092637803246646 1108 2 0.083224732714271 1108 3 -0.02642567620033276 1108 4 -0.14366515410812042 1108 5 0.09337408522017654 1108 6 0.049876422961183664 1108 7 0.017581183806171907 1108 8 0.13115329046512159 1108 9 0.05257233006629747 1108 10-11 -0.03223807055277916 1108 12-13 0.06367950992882498 1108 14-15 0.09255592417466829 1108 16-17 0.11811010971898384 1108 18-19 0.1562039335462586 1108 20-21 -0.013685104020346728 1108 22-23 0.10072502379266268 1108 24-25 0.14271269847312595 1108 26-27 -0.027785323310396848 1108 28-29 0.07641285479324722 1108 30-31 0.05960494338897604 1108 32-33 0.22343607203922744 1108 34-35 0.21779499954981674 1108 36-37 0.2690281384013673 1108 38-39 0.14418628580766324 1108 40-41 0.07337157782394854 1108 42-43 0.13443119861485542 1108 44-45 0.03444830724468062 1108 46-47 0.05125219525567104 1108 48-49 0.07388676484293555 1108 50-51 0.1825560246486546 1108 52-53 0.09908126517583327 1108 54-55 0.19805805304320856 1108 56-57 0.23180817092869077 1108 58-59 0.13731055575425444 1108 60-61 0.07700884036877653 1108 62-63 0.06642587950805634 1108 64-65 -0.05769292590173691 1108 66-67 -0.09259326030145232 1108 68-69 -0.13505565302078892 1108 70-71 -0.16756808040044646 1108 72-73 -0.11617553450649609 1108 74-75 -0.24667516062841344 1108 76-77 -0.23025606761255446 1109 1 0.12863488050866323 1109 2 -0.0016852258111086371 1109 3 -0.1685640459299691 1109 4 0.137577170716483 1109 5 0.062267016241470685 1109 6 0.09817694057573334 1109 7 -0.09544816995162364 1109 8 0.07891051003682747 1109 9 0.11739531982659202 1109 10-11 -0.10578500238776911 1109 12-13 -0.006281666340662184 1109 14-15 0.052831303829215415 1109 16-17 0.1863814157179533 1109 18-19 -0.006387317960701466 1109 20-21 -0.03293452789331042 1109 22-23 0.013102414140405472 1109 24-25 0.07257636384368027 1109 26-27 0.03997058521298413 1109 28-29 0.07414875303363289 1109 30-31 0.11937655800257829 1109 32-33 0.08666724181968988 1109 34-35 0.18140097942742983 1109 36-37 0.21383511532835797 1109 38-39 0.13328788466277786 1109 40-41 0.18152898577967136 1109 42-43 0.2512836595130281 1109 44-45 0.11536143992747583 1109 46-47 0.015342811921556176 1109 48-49 0.07748091841199312 1109 50-51 0.19229051048880308 1109 52-53 0.249254988676892 1109 54-55 0.3630162938505386 1109 56-57 0.3778968175669064 1109 58-59 0.3934205352410487 1109 60-61 0.3358216813097279 1109 62-63 0.2970907240159022 1109 64-65 0.2827350248423883 1109 66-67 0.34886900775648755 1109 68-69 0.35397516266145956 1109 70-71 0.38398203269426645 1109 72-73 0.4854141547798534 1109 74-75 0.35726059493228846 1109 76-77 0.416939003997463 1110 1 -0.17833026596834856 1110 2 -0.22181414811006306 1110 3 -0.28558870160622973 1110 4 -0.1267887073435645 1110 5 -0.08099122525852209 1110 6 -0.25614143979522197 1110 7 -0.39250242169049443 1110 8 -0.30255668100810595 1110 9 -0.20237598027390646 1110 10-11 -0.2752670377195159 1110 12-13 -0.1874929693209353 1110 14-15 -0.3583225490678359 1110 16-17 -0.3647024738626925 1110 18-19 -0.2094974784300021 1110 20-21 -0.26434093398231795 1110 22-23 -0.34181854676548085 1110 24-25 -0.370242599570453 1110 26-27 -0.3655397230501123 1110 28-29 -0.16626227562164786 1110 30-31 -0.22334446684035925 1110 32-33 -0.08515102291249121 1110 34-35 -0.01595209592949942 1110 36-37 -0.15073445481625214 1110 38-39 -0.1114455623944437 1110 40-41 -0.002327320748101158 1110 42-43 0.005524384724743925 1110 44-45 -0.31591588905585155 1110 46-47 -0.25603711662115103 1110 48-49 -0.21838486527189716 1110 50-51 -0.1405313700063715 1110 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7 -0.5488429969433 2314 8 -0.4262335749381805 2314 9 -0.4254703630704455 2314 10-11 -0.35937838530704624 2314 12-13 -0.5097984708833181 2314 14-15 -0.5883507827120056 2314 16-17 -0.8922997845927085 2314 18-19 -0.6029449970679011 2314 20-21 -0.36422652313665793 2314 22-23 -0.31315986015277986 2314 24-25 -0.5346842008302062 2314 26-27 -0.49411782998119946 2314 28-29 -0.605673428833768 2314 30-31 -0.6555188265086898 2314 32-33 -0.7069890392990814 2314 34-35 -0.656658675168238 2314 36-37 -0.7950563552665173 2314 38-39 -0.6815501929581345 2314 40-41 -0.6243609427740395 2314 42-43 -0.5494906240098487 2314 44-45 -0.38885649383404086 2314 46-47 -0.36158296707510473 2314 48-49 -0.30994306717628817 2314 50-51 -0.27410326430550924 2314 52-53 -0.393916766027381 2314 54-55 -0.47995243349015837 2314 56-57 -0.2408003107238308 2314 58-59 -0.26153764273338354 2314 60-61 -0.29523626850062 2314 62-63 -0.21430523412823632 2314 64-65 -0.19043188782039522 2314 66-67 -0.2615305452610457 2314 68-69 0.00234139663277233 2314 70-71 0.11684273658691424 2314 72-73 0.26471491081508347 2314 74-75 0.1660378223519423 2314 76-77 0.20333399534463226 2315 1 -0.9161559730034128 2315 2 -0.8383008623415478 2315 3 -0.8310530408999597 2315 4 -0.7387801781615835 2315 5 -0.7753297037174249 2315 6 -0.8445110647517069 2315 7 -0.9838641539429247 2315 8 -0.9967423355132823 2315 9 -0.8327136440914202 2315 10-11 -0.9417164037283143 2315 12-13 -0.9633795922042978 2315 14-15 -1.3342070157326944 2315 16-17 -1.2117253027080466 2315 18-19 -1.138308901540512 2315 20-21 -1.0281970773793532 2315 22-23 -1.0533707706923963 2315 24-25 -1.2206591904284707 2315 26-27 -1.2075991965928452 2315 28-29 -1.110947008146681 2315 30-31 -1.094597919150786 2315 32-33 -1.053019314476046 2315 34-35 -1.1038985433313044 2315 36-37 -1.3224436561718065 2315 38-39 -1.1674030131209179 2315 40-41 -1.2163828435319317 2315 42-43 -1.2872894190398583 2315 44-45 -1.1380480476225827 2315 46-47 -1.0206022939126775 2315 48-49 -1.016894351688979 2315 50-51 -0.958418020904837 2315 52-53 -1.0024371493552309 2315 54-55 -1.1460307334246203 2315 56-57 -1.2014105596313094 2315 58-59 -0.9978096937386134 2315 60-61 -1.04792487988896 2315 62-63 -1.1446725990484055 2315 64-65 -1.2969687684410722 2315 66-67 -1.225948952122657 2315 68-69 -1.2022598088810987 2315 70-71 -1.2780898492716162 2315 72-73 -2.6129110521955212 2315 74-75 -3.2037555297815707 2315 76-77 -2.925870618404158 2316 1 -3.085353574043779 2316 2 -2.933598891760859 2316 3 -2.5415750122423546 2316 4 -2.5724787901080717 2316 5 -3.2626819398833646 2316 6 -2.849331021953695 2316 7 -3.121119733673126 2316 8 -2.9912157254909317 2316 9 -2.718473471441655 2316 10-11 -2.6349498197729844 2316 12-13 -2.5885257655611937 2316 14-15 -2.6946785492999226 2316 16-17 -3.8180890648381975 2316 18-19 -3.616028848208394 2316 20-21 -3.0034100331815594 2316 22-23 -3.179260313724356 2316 24-25 -3.327719477939816 2316 26-27 -3.002437651425595 2316 28-29 -2.9602056678696513 2316 30-31 -3.0478420741911663 2316 32-33 -3.1472882162072757 2316 34-35 -3.1848587387416103 2316 36-37 -3.011990254239869 2316 38-39 -3.1762323413713958 2316 40-41 -3.1351938227395593 2316 42-43 -3.322320074680885 2316 44-45 -3.0801921383243993 2316 46-47 -2.7779063477115145 2316 48-49 -2.8816410722560413 2316 50-51 -3.3873465073422366 2316 52-53 -4.480317336353899 2316 54-55 -3.715343318046415 2316 56-57 -3.5697557665724275 2316 58-59 -3.77985269617475 2316 60-61 -3.4355569931148224 2316 62-63 -3.369919710518978 2316 64-65 -3.4761803343988937 2316 66-67 -3.5544893738605055 2316 68-69 -3.525010932811785 2316 70-71 -3.336651277429791 2316 72-73 -3.3417820663932503 2316 74-75 -3.437745710310409 2316 76-77 -3.3753061915855405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75699.0 3 6985.0 4 8761.0 5 10313.0 6 12001.0 7 12621.0 8 14445.0 9 15141.0 10 18133.0 11 20050.0 12 16241.0 13 14291.0 14 14172.0 15 14891.0 16 15202.0 17 16362.0 18 17339.0 19 18349.0 20 19477.0 21 21447.0 22 23727.0 23 26954.0 24 31838.0 25 37230.0 26 44073.0 27 52028.0 28 61709.0 29 73423.0 30 87284.0 31 105761.0 32 128514.0 33 162889.0 34 217095.0 35 290194.0 36 408235.0 37 544351.0 38 481313.0 39 127349.0 40 906.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.833456299920098 26.522362060573034 28.255364353568226 22.388817285938643 2 22.60371815407525 26.670916055587394 28.25277251196442 22.472593278372937 3 22.727325608450837 26.86597916681974 27.791575126564144 22.615120098165278 4 22.426010901658515 26.80960550985644 28.217134291728968 22.54724929675608 5 22.69798836886068 26.890764277780704 28.027151570963056 22.384095782395562 6 22.817099752638924 26.75335529377189 28.086043692292623 22.343501261296563 7 22.6854554845299 26.471365340699126 28.1488144424112 22.694364732359766 8 22.635078246461198 26.768727623202125 27.85824461196405 22.737949518372623 9 22.555992043409162 26.68988002350115 28.007242616318607 22.746885316771085 10-11 23.01515873571114 26.719106810140975 27.736363743523885 22.529370710624004 12-13 23.06341432051821 26.47582133374359 27.71246602571904 22.748298320019167 14-15 22.70140774917944 26.752744849919324 27.846965270295527 22.698882130605703 16-17 22.944428568170466 26.513896263660502 27.964343130176385 22.577332037992644 18-19 22.892571446766404 26.58210989624666 27.627236232352626 22.898082424634318 20-21 22.848097748584166 26.70339648222304 27.612471664597628 22.836034104595164 22-23 22.947803350162733 26.762019650287343 27.30342823726084 22.986748762289082 24-25 22.96711482485825 26.871014269561748 27.166205935184895 22.995664970395108 26-27 22.955638810437694 26.885206649830618 27.13165430090802 23.027500238823663 28-29 23.16232149466447 26.818575484027395 27.125708595043196 22.89339442626494 30-31 22.900069523934018 26.670327056382348 27.260652912703836 23.168950506979794 32-33 22.837000326961864 27.071294217918247 27.128558465556956 22.963146989562937 34-35 22.93343779074413 26.70590554809067 27.273135005080512 23.08752165608469 36-37 22.97254105738298 26.882375848180445 27.332151809757764 22.812931284678807 38-39 22.84501695330379 26.755038154500856 27.470429305105892 22.929515587089465 40-41 22.725656407202 26.72663791634732 27.34109141918844 23.206614257262242 42-43 22.663852874123453 26.99468188923126 27.11748170670009 23.2239835299452 44-45 22.751443430298828 26.66764839418666 27.450203606409723 23.13070456910479 46-47 22.65945930719515 26.689433463762473 27.457617673656276 23.193489555386098 48-49 22.811111549975244 26.668130227522475 27.312678754209653 23.208079468292627 50-51 22.64330371011618 26.86730306337943 27.19025803785523 23.29913518864916 52-53 22.73292684383288 26.758088525536966 27.265373580509817 23.243611050120332 54-55 22.66149216476019 26.729132615161706 27.309067126041754 23.30030809403635 56-57 22.603717496931733 26.88575701475967 27.286158656414056 23.224366831894535 58-59 22.716126376557614 26.879993582863797 27.231956358575243 23.171923682003346 60-61 22.663416127674232 26.67535688315665 27.180894791228138 23.480332197940985 62-63 22.685925215791393 26.538073913403586 27.338026515155573 23.437974355649445 64-65 22.62824567860334 26.555378238176974 27.32852045249816 23.487855630721523 66-67 22.57175458373056 26.60381769460311 27.46224093630138 23.362186785364948 68-69 22.60680055698995 26.439306684093783 27.477277731518473 23.47661502739779 70-71 22.499194493891018 26.279269274260436 27.581769589724804 23.639766642123742 72-73 22.65039174818066 26.091274430836908 27.511803916654593 23.746529904327843 74-75 22.45671279168984 26.11007198322281 27.495477112889116 23.937738112198236 76-77 22.312183766049536 25.98369937882261 27.839954752157148 23.864162102970706 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8933.0 1 5396.5 2 1728.5 3 1613.5 4 1630.0 5 1672.0 6 1797.5 7 1893.5 8 1949.5 9 1993.0 10 2111.5 11 2259.5 12 2466.0 13 2643.0 14 2759.5 15 3095.5 16 3553.5 17 3792.0 18 4062.5 19 4731.5 20 5841.0 21 7532.5 22 8513.0 23 9988.0 24 13241.0 25 17904.0 26 20789.0 27 24029.0 28 32447.5 29 41438.5 30 45251.0 31 52113.5 32 65738.5 33 78415.0 34 89755.5 35 95182.0 36 101971.0 37 113198.5 38 119385.0 39 121133.0 40 125062.0 41 132410.5 42 140884.5 43 145939.0 44 146419.0 45 148920.0 46 153879.5 47 158698.0 48 160578.0 49 161896.0 50 161947.0 51 156080.5 52 151481.0 53 148095.5 54 138874.5 55 124924.5 56 108912.0 57 101014.0 58 95385.0 59 81608.5 60 67018.0 61 60575.0 62 53414.5 63 39282.5 64 28383.0 65 24455.0 66 21143.5 67 15889.0 68 11799.0 69 8096.5 70 6541.0 71 5588.5 72 3954.5 73 2897.0 74 2521.0 75 2151.5 76 1435.5 77 894.5 78 700.0 79 554.0 80 361.5 81 255.0 82 169.5 83 144.0 84 124.0 85 100.5 86 84.5 87 72.0 88 77.0 89 73.0 90 64.0 91 64.0 92 54.0 93 48.5 94 41.5 95 35.5 96 41.0 97 41.5 98 44.0 99 94.0 100 142.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009887372723034487 2 0.013499477928353588 3 0.014234143393842219 4 0.015336141592075164 5 0.01190770275312822 6 0.009581262112414224 7 0.016040195996501767 8 0.01760136011066511 9 0.017448304805354975 10-11 0.02009616158722025 12-13 0.01639222319871507 14-15 0.008157847773030002 16-17 0.00653546153674261 18-19 0.01790747072128537 20-21 0.023922544219973533 22-23 0.020907354705363944 24-25 0.01867274724783603 26-27 0.024029682933690625 28-29 0.008999651952235725 30-31 0.008938429830111673 32-33 0.01092814879914338 34-35 0.011938313814190247 36-37 0.01190770275312822 38-39 0.014325976577028297 40-41 0.012076063588969366 42-43 0.015351447122606178 44-45 0.013530088989415613 46-47 0.023861322097849483 48-49 0.024060293994752652 50-51 0.03573841378991568 52-53 0.038937269670897424 54-55 0.02776423238325783 56-57 0.021351215090763327 58-59 0.01648405638190115 60-61 0.020448188789433552 62-63 0.02600409637219132 64-65 0.025560235986791937 66-67 0.02820809276865721 68-69 0.04236570850984436 70-71 0.054028522774476374 72-73 0.08675174704978247 74-75 0.08687419129403057 76-77 0.09155768363652059 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3266793.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.898903158949906 #Duplication Level Percentage of deduplicated Percentage of total 1 71.64153021084641 35.031922479391625 2 13.686383497400728 13.384982824712969 3 5.613549729171963 8.234892737541879 4 2.9040375127814233 5.680169964298262 5 1.6679286435989542 4.077994060969196 6 1.0551590855385424 3.095767316462119 7 0.6924295036821961 2.370133027146882 8 0.4877546482590584 1.9080533848437897 9 0.36521760968830996 1.6072866475282652 >10 1.712024626871668 14.622151204545334 >50 0.11500415620145099 3.866819898699466 >100 0.056468020092805306 4.879075038076806 >500 0.002073023590407087 0.6944054891339391 >1k 3.769133796006216E-4 0.2760297142062537 >5k 6.28188966001036E-5 0.27031621244324866 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8800 0.2693773373458312 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.122212212405255E-5 0.0 2 0.0 0.0 0.0 2.448884884962102E-4 0.0 3 0.0 0.0 0.0 4.2855485486836787E-4 0.0 4 0.0 0.0 0.0 0.0011632203203569985 0.0 5 0.0 0.0 0.0 0.002785606556644391 0.0 6 0.0 0.0 0.0 0.006458933884087544 0.0 7 0.0 0.0 0.0 0.013468866867291561 0.0 8 0.0 0.0 0.0 0.020999187888550024 6.122212212405255E-5 9 0.0 0.0 0.0 0.030029450901847776 9.183318318607883E-5 10 0.0 0.0 0.0 0.04055965590718481 1.224442442481051E-4 11 0.0 0.0 0.0 0.05216124804969277 1.224442442481051E-4 12 0.0 0.0 0.0 0.06786472237451226 1.224442442481051E-4 13 0.0 0.0 0.0 0.08953735360642685 1.224442442481051E-4 14 0.0 0.0 0.0 0.11469964579941246 1.224442442481051E-4 15 0.0 0.0 0.0 0.14231082287736016 1.224442442481051E-4 16 0.0 0.0 0.0 0.17111583133672686 1.224442442481051E-4 17 0.0 0.0 0.0 0.19894128584210877 1.224442442481051E-4 18 0.0 0.0 0.0 0.22936868053776288 1.224442442481051E-4 19 0.0 0.0 0.0 0.26129601722545626 1.5305530531013137E-4 20 0.0 0.0 0.0 0.29845784535475617 1.5305530531013137E-4 21 0.0 0.0 0.0 0.33509928544600165 1.5305530531013137E-4 22 0.0 0.0 0.0 0.3757201634753105 1.5305530531013137E-4 23 0.0 0.0 0.0 0.4199837577710005 1.5305530531013137E-4 24 0.0 0.0 0.0 0.4610944127773018 1.8366636637215765E-4 25 0.0 0.0 0.0 0.5069497822482172 1.8366636637215765E-4 26 0.0 0.0 0.0 0.5544275379554199 1.8366636637215765E-4 27 0.0 0.0 0.0 0.5992115202891644 1.8366636637215765E-4 28 0.0 0.0 0.0 0.6463219432636228 1.8366636637215765E-4 29 0.0 0.0 0.0 0.6950547524743685 3.0611061062026275E-4 30 0.0 0.0 0.0 0.7472160005240613 3.0611061062026275E-4 31 0.0 0.0 0.0 0.8068463474728885 3.0611061062026275E-4 32 0.0 0.0 0.0 0.8688031350624297 3.0611061062026275E-4 33 3.0611061062026274E-5 0.0 0.0 0.9356270813608331 3.0611061062026275E-4 34 3.0611061062026274E-5 0.0 0.0 1.0069508536353544 3.0611061062026275E-4 35 3.0611061062026274E-5 0.0 0.0 1.075213519803673 3.0611061062026275E-4 36 3.0611061062026274E-5 0.0 0.0 1.149843286672893 3.0611061062026275E-4 37 3.0611061062026274E-5 0.0 0.0 1.2258505512899043 3.0611061062026275E-4 38 3.0611061062026274E-5 0.0 0.0 1.3022251486396597 3.673327327443153E-4 39 3.0611061062026274E-5 0.0 0.0 1.385089290934565 3.673327327443153E-4 40 3.0611061062026274E-5 0.0 0.0 1.4734328131595726 3.673327327443153E-4 41 3.0611061062026274E-5 0.0 0.0 1.5676536591084895 3.673327327443153E-4 42 3.0611061062026274E-5 0.0 0.0 1.6698027698724713 3.673327327443153E-4 43 3.0611061062026274E-5 0.0 0.0 1.7846554709771938 3.673327327443153E-4 44 3.0611061062026274E-5 0.0 0.0 1.9017121684783824 3.673327327443153E-4 45 3.0611061062026274E-5 0.0 0.0 2.0196871978114315 3.9794379380634156E-4 46 3.0611061062026274E-5 0.0 0.0 2.146233324241848 4.2855485486836787E-4 47 3.0611061062026274E-5 0.0 0.0 2.272932505977575 4.2855485486836787E-4 48 3.0611061062026274E-5 0.0 0.0 2.4040396805062336 4.897769769924204E-4 49 3.0611061062026274E-5 0.0 0.0 2.542095565895972 4.897769769924204E-4 50 3.0611061062026274E-5 0.0 0.0 2.685692053337937 4.897769769924204E-4 51 3.0611061062026274E-5 0.0 0.0 2.838104526365766 4.897769769924204E-4 52 3.0611061062026274E-5 0.0 0.0 3.0037103667113283 4.897769769924204E-4 53 3.0611061062026274E-5 0.0 0.0 3.1749792533533654 4.897769769924204E-4 54 3.0611061062026274E-5 0.0 0.0 3.3630230014573925 4.897769769924204E-4 55 3.0611061062026274E-5 0.0 0.0 3.550730027889738 4.897769769924204E-4 56 3.0611061062026274E-5 0.0 0.0 3.7528854751433594 4.897769769924204E-4 57 3.0611061062026274E-5 0.0 0.0 3.960887635059828 4.897769769924204E-4 58 3.0611061062026274E-5 0.0 0.0 4.172991677158608 5.50999099116473E-4 59 3.0611061062026274E-5 0.0 0.0 4.388279269607839 5.50999099116473E-4 60 3.0611061062026274E-5 0.0 0.0 4.60978090745266 5.50999099116473E-4 61 3.0611061062026274E-5 0.0 0.0 4.8532000650178935 5.50999099116473E-4 62 3.0611061062026274E-5 0.0 0.0 5.090680676737094 5.816101601784992E-4 63 3.0611061062026274E-5 0.0 0.0 5.34805847814661 5.816101601784992E-4 64 3.0611061062026274E-5 0.0 0.0 5.615078763790666 5.816101601784992E-4 65 3.0611061062026274E-5 0.0 0.0 5.899792242728572 5.816101601784992E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACCG 50 0.006731654 28.398329 56 ATTCGCG 470 0.0 24.168789 56 CGCGGAA 490 0.0 22.459581 59 TCGCGCT 520 0.0 21.844198 37 CTCGCGC 555 0.0 21.105251 36 ATGCGCG 85 0.003496074 20.879206 27 AATTCGC 535 0.0 20.56857 55 CCTCGCG 600 0.0 20.11271 35 CAATTCG 565 0.0 20.107166 54 CGGTTAC 330 0.0 19.358643 4 CGCGCTT 610 0.0 18.620428 38 CGATCGT 580 0.0 17.74651 1 GACGTAT 445 0.0 17.548183 39 TCGCGGA 635 0.0 17.329687 58 CGCTCCG 650 0.0 16.929255 25 CGCGTTA 155 3.6413738E-4 16.033033 54 AACTCGA 690 0.0 15.947604 13 ACTCGAG 735 0.0 15.453689 14 CGGCCAA 715 0.0 15.394944 50 ACGCTCC 765 0.0 14.849481 24 >>END_MODULE