FastQCFastQC Report
Thu 26 May 2016
SRR936125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3266793
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81200.24856181582365333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58600.17938081782347398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42210.1292092887428129No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA15000.045.43691100-101
CGGTATC12000.038.19478100-101
GGTATCA85650.037.5858841
CGTACAT21200.036.50077100-101
CCTGTCG10150.035.47404596-97
TACGCAG10100.034.18229100-101
GTATCAA166600.030.4312291
ACGTATC5850.030.016546100-101
AGGTATC23350.028.93367100-101
GCGTATC4100.028.310352100-101
GCGTACA8350.028.15822100-101
AGTATCA33050.027.735998100-101
CCGTACA10400.027.472769100-101
CCGTATC7500.027.381193100-101
TACCGTA2550.026.84389798-99
TATACGT5450.026.20813696-97
ACCGTAT4100.026.13223398-99
CGAGTAC14350.025.925232100-101
TCGGTAT4400.025.70369100-101
TCGCATA1551.2732926E-1124.961819100-101
Produced by FastQC (version 0.11.5)