FastQCFastQC Report
Thu 26 May 2016
SRR936124_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936124_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3270710
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89200.27272365938894No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC3500.018.2521614
TCGCGCT5500.018.07000537
ATTCGCG4400.017.75058656
TGCGTAA4750.017.18688637
CGCTCCG5500.016.78108825
TTGCGTA4900.016.65973736
CTCGCGC5550.016.6271136
CAATTCG5150.016.54372854
GCGTAAG5250.016.22687138
CGCGGAA4750.015.69597459
CGCGCTT6350.015.65190438
CGATCGT5900.015.6395611
AATTCGC5550.015.35185755
CCTCGCG6300.015.20943635
CGTAAGT5550.014.71039739
TCGCGGA5650.014.45290958
CGACTTA2951.0884105E-714.44933169
GATCGTT6550.014.0877592
TAAGGCG9500.013.82529217
ATCGTTC7050.013.0886273
Produced by FastQC (version 0.11.5)