##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936124_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3270710 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.65534088928703 39.0 36.0 41.0 30.0 41.0 2 36.52489551198364 39.0 36.0 41.0 30.0 41.0 3 36.39429206502564 39.0 36.0 41.0 30.0 41.0 4 36.286520969453115 39.0 36.0 41.0 29.0 41.0 5 36.21400093557668 39.0 36.0 41.0 29.0 41.0 6 36.12332704519814 39.0 35.0 40.0 29.0 41.0 7 35.98266186852396 39.0 35.0 40.0 29.0 41.0 8 35.85821641172712 39.0 35.0 40.0 28.0 41.0 9 36.020309046048105 39.0 35.0 40.0 29.0 41.0 10-11 36.24413399537104 39.0 35.0 41.0 30.0 41.0 12-13 36.167029941511174 39.0 35.0 41.0 29.0 41.0 14-15 36.015747345377605 39.0 35.0 41.0 28.5 41.0 16-17 35.86823839472164 39.0 35.0 41.0 28.0 41.0 18-19 35.715471411406085 39.0 35.0 41.0 27.0 41.0 20-21 35.51600478183636 39.0 35.0 41.0 27.0 41.0 22-23 35.24940486927915 39.0 35.0 41.0 26.0 41.0 24-25 35.02934699193753 39.0 34.5 41.0 25.0 41.0 26-27 34.06292456377973 38.0 33.0 40.0 23.5 40.5 28-29 34.062550944596126 38.0 33.5 39.5 23.5 40.5 30-31 34.46724686688823 38.0 34.0 40.0 23.5 41.0 32-33 34.34193279135111 38.0 34.0 40.0 23.0 41.0 34-35 34.08156057858997 38.0 33.5 40.0 22.5 41.0 36-37 33.77144289771945 37.5 33.0 40.0 22.0 41.0 38-39 33.45772676269067 37.0 33.0 40.0 21.5 41.0 40-41 33.09708549519829 36.5 32.5 39.5 20.0 41.0 42-43 32.72791809729386 36.0 32.0 39.0 18.5 41.0 44-45 32.36980273396296 35.5 32.0 39.0 18.0 40.5 46-47 31.95240238358032 35.0 31.5 38.5 14.5 40.0 48-49 31.54136517759141 35.0 31.0 37.5 10.5 40.0 50-51 31.097862237862728 35.0 31.0 37.0 7.5 39.0 52-53 30.619493168150036 35.0 30.5 36.5 4.5 39.0 54-55 30.165126990775704 34.5 30.0 36.0 2.0 38.5 56-57 29.739798698141996 34.0 30.0 35.5 2.0 37.0 58-59 29.31891668781396 34.0 29.0 35.0 2.0 37.0 60-61 28.973708613726075 34.0 29.0 35.0 2.0 36.0 62-63 28.617905286619724 34.0 29.0 35.0 2.0 36.0 64-65 28.25110450024612 34.0 29.0 35.0 2.0 36.0 66-67 27.9095019124288 34.0 27.5 35.0 2.0 35.0 68-69 27.55319640078148 34.0 27.0 35.0 2.0 35.0 70-71 27.11207673563232 33.0 26.0 35.0 2.0 35.0 72-73 26.63782542628359 33.0 25.0 35.0 2.0 35.0 74-75 26.08135894041355 33.0 24.5 35.0 2.0 35.0 76-77 24.312949023300753 31.0 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.1377607761399489 1101 2 1.0257355248587245 1101 3 1.1289367998926423 1101 4 1.0885970168580457 1101 5 1.1905536666364682 1101 6 1.1369479732450074 1101 7 1.1372926087949367 1101 8 1.100082310153745 1101 9 1.0959443027192677 1101 10-11 1.0901834254353702 1101 12-13 1.1743032705967238 1101 14-15 1.2434841717236438 1101 16-17 1.281759976075044 1101 18-19 1.196791837514887 1101 20-21 1.1841813184234695 1101 22-23 1.2000542276912327 1101 24-25 1.2215778790352658 1101 26-27 1.2584978566622809 1101 28-29 1.3758936797660297 1101 30-31 1.2734869468392205 1101 32-33 1.2370161369455843 1101 34-35 1.1889405826749524 1101 36-37 1.1411292330278187 1101 38-39 1.0752897920010653 1101 40-41 1.0548934965512657 1101 42-43 1.0756998725633906 1101 44-45 1.1603734060233606 1101 46-47 1.126855352829491 1101 48-49 1.1754668416220575 1101 50-51 1.1012110925173317 1101 52-53 1.160919901521961 1101 54-55 1.2460522598368762 1101 56-57 1.2921654173883113 1101 58-59 1.3152218159012037 1101 60-61 1.2141066403240437 1101 62-63 1.294190133285099 1101 64-65 1.2252344583623334 1101 66-67 1.2821253775513206 1101 68-69 1.259289926431034 1101 70-71 1.1514256846123168 1101 72-73 1.1310931788432939 1101 74-75 1.1069913005738705 1101 76-77 1.3212334592755575 1102 1 0.6398895642667526 1102 2 0.7398053014905699 1102 3 0.853874887293486 1102 4 0.6534687555133019 1102 5 0.7069462445980577 1102 6 0.6174826652990646 1102 7 0.6110055889627333 1102 8 0.6392640817089017 1102 9 0.6374739415676771 1102 10-11 0.6792701083651522 1102 12-13 0.7745909339429744 1102 14-15 0.8508931480501971 1102 16-17 0.8619846173721797 1102 18-19 0.9325023368798142 1102 20-21 0.8860580580360633 1102 22-23 0.7479900720521044 1102 24-25 0.7726813601288143 1102 26-27 0.5968754671352201 1102 28-29 0.7911594798381927 1102 30-31 0.8541609897774194 1102 32-33 0.7770079227169049 1102 34-35 0.7198173523799198 1102 36-37 0.8211131021874536 1102 38-39 0.9257046866066219 1102 40-41 0.9100896079539282 1102 42-43 0.8865517151927875 1102 44-45 0.8263904409579723 1102 46-47 0.8930828360381078 1102 48-49 0.8006637235220175 1102 50-51 0.7849345190411583 1102 52-53 0.803786138231299 1102 54-55 0.801885205441419 1102 56-57 0.8301762314666554 1102 58-59 1.0097373301783819 1102 60-61 0.8260541102655523 1102 62-63 0.8084269722488493 1102 64-65 0.7321767051258306 1102 66-67 0.741071925872248 1102 68-69 0.7003341519837001 1102 70-71 0.5934563451136796 1102 72-73 0.6947415463645505 1102 74-75 0.6068659707563846 1102 76-77 0.5811812241885654 1103 1 0.5627529981098363 1103 2 0.52159982262841 1103 3 0.5416531899414849 1103 4 0.7084608367875731 1103 5 0.5203262812316822 1103 6 0.5662899847157945 1103 7 0.5533125284285205 1103 8 0.4212714145228773 1103 9 0.6045112942034763 1103 10-11 0.6561668835016121 1103 12-13 0.7439435514722206 1103 14-15 0.6961557883243046 1103 16-17 0.6151061633962698 1103 18-19 0.6601046963599444 1103 20-21 0.7094615572385337 1103 22-23 0.6790544762381359 1103 24-25 0.8254566984199982 1103 26-27 0.8677617858999298 1103 28-29 0.8281081866783069 1103 30-31 0.8695660757698604 1103 32-33 0.8748359386944173 1103 34-35 1.03945575956989 1103 36-37 1.0786015285815793 1103 38-39 0.9850528826117184 1103 40-41 1.0537883270821027 1103 42-43 0.8977709792928366 1103 44-45 0.8851331513153013 1103 46-47 0.8980208933774243 1103 48-49 0.9141697725063302 1103 50-51 0.8749676141385052 1103 52-53 0.8435935572573818 1103 54-55 0.8766465888543316 1103 56-57 0.8853643778671696 1103 58-59 0.8391465880191404 1103 60-61 0.7844713938359718 1103 62-63 0.8208525860316485 1103 64-65 0.8146886419360975 1103 66-67 0.7831762320844327 1103 68-69 0.7834207999377298 1103 70-71 0.7884778024463017 1103 72-73 0.8935685484145885 1103 74-75 0.7825913247284255 1103 76-77 0.7347407251152802 1104 1 0.4489016546475497 1104 2 0.48997607698296264 1104 3 0.6200782916803362 1104 4 0.6845749753561989 1104 5 0.5415143914259488 1104 6 0.4742297544686309 1104 7 0.38385509316032795 1104 8 0.3741779506468035 1104 9 0.4489225038396327 1104 10-11 0.5176118935746672 1104 12-13 0.48934816108587853 1104 14-15 0.6300106769900538 1104 16-17 0.6427902220810608 1104 18-19 0.4885435967949192 1104 20-21 0.4941437308091494 1104 22-23 0.5543515727801847 1104 24-25 0.5522060015858443 1104 26-27 0.34361661512514274 1104 28-29 0.462514651685872 1104 30-31 0.5970715474835941 1104 32-33 0.5124064977963627 1104 34-35 0.5008171283156315 1104 36-37 0.5649539812467523 1104 38-39 0.5238719583538298 1104 40-41 0.521405175502359 1104 42-43 0.5355396558280461 1104 44-45 0.5610276252559316 1104 46-47 0.37018934785976754 1104 48-49 0.27645804212860625 1104 50-51 0.22403678594076482 1104 52-53 0.2500439169787718 1104 54-55 0.2106503972944651 1104 56-57 0.1814926760209019 1104 58-59 0.20513540258377105 1104 60-61 0.18970858280805203 1104 62-63 0.02233361070159745 1104 64-65 -0.020386210683216888 1104 66-67 0.01423688478398688 1104 68-69 -0.00929194942063205 1104 70-71 0.022911334127044825 1104 72-73 -0.19805925192464713 1104 74-75 -0.19296980455686352 1104 76-77 -0.19567908429288394 1105 1 0.051776072813119356 1105 2 0.22179206021780828 1105 3 0.13188358606279849 1105 4 0.1293683724267609 1105 5 0.08788944427384848 1105 6 0.22018801312972158 1105 7 0.17432343847421095 1105 8 0.0352633716307551 1105 9 0.024191361974807535 1105 10-11 0.24469448145443806 1105 12-13 0.3476272761544692 1105 14-15 0.2717044591333817 1105 16-17 0.32749871738401026 1105 18-19 0.1942201082702084 1105 20-21 0.1729329061382998 1105 22-23 0.12595490571255397 1105 24-25 0.3308572267688987 1105 26-27 0.17724985552737849 1105 28-29 0.28845410080901246 1105 30-31 0.4086307850933295 1105 32-33 0.3492221841186449 1105 34-35 0.23018695804007905 1105 36-37 0.17358687905755943 1105 38-39 0.10169066100325352 1105 40-41 0.3043356974543556 1105 42-43 0.3898167955398719 1105 44-45 0.18342544038547715 1105 46-47 0.180179973543364 1105 48-49 -0.03276507726019062 1105 50-51 0.032026271683125884 1105 52-53 0.010429487129570703 1105 54-55 -0.09271202331948913 1105 56-57 -0.03573068901863152 1105 58-59 0.1096967409133569 1105 60-61 0.13554335629421743 1105 62-63 -0.10006217425656061 1105 64-65 -0.012991971193031304 1105 66-67 0.04752758451808958 1105 68-69 -0.006356930639011438 1105 70-71 -0.13187740877560117 1105 72-73 -0.5475693461905777 1105 74-75 -0.6189861464214879 1105 76-77 -0.5629883509594471 1106 1 0.08644819278421778 1106 2 0.045291265226651944 1106 3 -0.06029655558437241 1106 4 0.12372728422181467 1106 5 0.24288859904753934 1106 6 0.04275029275291331 1106 7 -0.2374262637031208 1106 8 -0.0915711083397781 1106 9 -0.040209201137280104 1106 10-11 -0.20878901709671993 1106 12-13 0.06938550435281599 1106 14-15 0.061957365196839476 1106 16-17 0.030921664640288782 1106 18-19 0.06665632159256774 1106 20-21 0.15124059163756698 1106 22-23 0.0740903544240652 1106 24-25 0.001244384272837351 1106 26-27 -0.20897296566260337 1106 28-29 -0.026343359844105407 1106 30-31 0.21122887918775035 1106 32-33 0.15243954602381393 1106 34-35 0.05637095264339109 1106 36-37 0.18746593355233898 1106 38-39 0.2239062981683304 1106 40-41 0.16511576159586383 1106 42-43 0.1776621023809497 1106 44-45 0.11798553796924693 1106 46-47 0.16114065297773905 1106 48-49 0.07550821311012967 1106 50-51 0.10945528064731036 1106 52-53 0.0462790432262814 1106 54-55 0.1310640331735229 1106 56-57 0.09806061144975331 1106 58-59 -0.08448782302163949 1106 60-61 -0.05656272837831722 1106 62-63 0.03902434940738431 1106 64-65 0.08362078438825549 1106 66-67 0.09892865675942275 1106 68-69 -0.06770182124599344 1106 70-71 -0.034984721817124864 1106 72-73 -0.31000284442278314 1106 74-75 -0.1952755949671392 1106 76-77 -0.20715191381159315 1107 1 -0.16939901502836818 1107 2 -0.08176536089375475 1107 3 -0.02000168166843963 1107 4 -0.1773232746486002 1107 5 -0.07965728800945726 1107 6 -0.23299338603537478 1107 7 -0.10770120336305666 1107 8 -0.16554403223165792 1107 9 -0.24163496236532467 1107 10-11 -0.09831890379190611 1107 12-13 -0.0018681060010905526 1107 14-15 -0.1498534785436405 1107 16-17 -0.10451579506213449 1107 18-19 -0.0027073129437482635 1107 20-21 -0.11920825575501937 1107 22-23 -0.09841986395982616 1107 24-25 -0.1280614652735892 1107 26-27 -0.34846980254418725 1107 28-29 -0.30205949712104996 1107 30-31 0.014062291892180667 1107 32-33 0.13162898678231016 1107 34-35 -0.026562819395614667 1107 36-37 -0.04486671842656165 1107 38-39 -0.05941168278570075 1107 40-41 -0.15260156702056094 1107 42-43 -0.07730455016384497 1107 44-45 -0.13607818786960024 1107 46-47 -0.17626334794379162 1107 48-49 -0.11340278200236398 1107 50-51 -0.2698084352186143 1107 52-53 -0.14742456140671933 1107 54-55 -0.19243604645526702 1107 56-57 -0.2589973501923666 1107 58-59 -0.2633346667064558 1107 60-61 -0.23989671239998955 1107 62-63 -0.059624850593852585 1107 64-65 -0.15999403027766945 1107 66-67 -0.23640939258259763 1107 68-69 -0.3568387920430993 1107 70-71 -0.3033430718621979 1107 72-73 -0.3340152734719837 1107 74-75 -0.45761786424851536 1107 76-77 -0.4248047952914824 1108 1 0.31913860883356904 1108 2 0.38981771459881287 1108 3 0.5177749354413024 1108 4 0.3489112640143972 1108 5 0.3953565392024956 1108 6 0.461560860357892 1108 7 0.43538267662184893 1108 8 0.22044769038754453 1108 9 0.2841705501560412 1108 10-11 0.3850004505155553 1108 12-13 0.45115799481987295 1108 14-15 0.45594659964660167 1108 16-17 0.4970759101409854 1108 18-19 0.29099478369152365 1108 20-21 0.2762208578314258 1108 22-23 0.3314776288514807 1108 24-25 0.3569249880665808 1108 26-27 0.35087587338240667 1108 28-29 0.5207323936836943 1108 30-31 0.5331768183711958 1108 32-33 0.533849238054998 1108 34-35 0.6155594681754195 1108 36-37 0.6464681923141384 1108 38-39 0.7180954512540936 1108 40-41 0.5199333908674717 1108 42-43 0.5528055519717014 1108 44-45 0.5229012290774975 1108 46-47 0.48430601620120584 1108 48-49 0.4764361573940086 1108 50-51 0.4833251407498018 1108 52-53 0.6569447100467727 1108 54-55 0.6019786799080329 1108 56-57 0.5241855901979591 1108 58-59 0.5624356766019005 1108 60-61 0.5700911163743072 1108 62-63 0.5219568840781648 1108 64-65 0.5707809075272046 1108 66-67 0.6144591621877353 1108 68-69 0.5762350427182561 1108 70-71 0.6790663553450926 1108 72-73 0.7039611003954249 1108 74-75 0.6825144616233452 1108 76-77 0.6915741931031327 1109 1 -0.24507881018267597 1109 2 -0.23058224841596342 1109 3 -0.36370926470576137 1109 4 -0.09680999897976506 1109 5 -0.40054634242871145 1109 6 -0.38446065173832267 1109 7 -0.32885352737869766 1109 8 -0.11530969167272076 1109 9 -0.26628741606607065 1109 10-11 -0.32229349574915744 1109 12-13 -0.27298195258217817 1109 14-15 -0.11437169071516706 1109 16-17 -0.15915673019454601 1109 18-19 -0.15801376018713853 1109 20-21 -0.19481518156295152 1109 22-23 -0.1327557976027478 1109 24-25 -0.4117083275781681 1109 26-27 -0.5404384651887995 1109 28-29 -0.3038283702584934 1109 30-31 -0.14822272143323545 1109 32-33 -0.20516908388308508 1109 34-35 -0.16893267758144503 1109 36-37 -0.11598380364525696 1109 38-39 -0.16193876007625363 1109 40-41 -0.19282670258964885 1109 42-43 -0.20256127966659676 1109 44-45 -0.1737048286048264 1109 46-47 -0.2727479464071685 1109 48-49 -0.18527354844922073 1109 50-51 -0.22236755920064155 1109 52-53 -0.1650857457485806 1109 54-55 -0.09046777375006698 1109 56-57 -0.23473343994134055 1109 58-59 -0.15912266027645572 1109 60-61 -0.22619290125872737 1109 62-63 -0.27328852310281704 1109 64-65 -0.1152519724082417 1109 66-67 -0.15534396642581427 1109 68-69 -0.3539336968750817 1109 70-71 -0.34059405855316527 1109 72-73 -0.5460452952704316 1109 74-75 -0.41933907543570115 1109 76-77 -0.46396881171956394 1110 1 -0.48544993025814875 1110 2 -0.5112426749916281 1110 3 -0.7312814990296843 1110 4 -0.4656289673153964 1110 5 -0.5366744467562725 1110 6 -0.5243656110107864 1110 7 -0.4232612564874785 1110 8 -0.7397013737630402 1110 9 -0.7310888509338511 1110 10-11 -0.5128301692594874 1110 12-13 -0.5620445905322242 1110 14-15 -0.41914304344297904 1110 16-17 -0.3941220811555226 1110 18-19 -0.5247016427351596 1110 20-21 -0.6631081405122288 1110 22-23 -0.623607953676725 1110 24-25 -0.5961352018126718 1110 26-27 -0.6655795253835777 1110 28-29 -0.4913209794801503 1110 30-31 -0.4816698928184948 1110 32-33 -0.4865224377313524 1110 34-35 -0.6140220705503978 1110 36-37 -0.4930841892766793 1110 38-39 -0.5263798460083322 1110 40-41 -0.49987441504676866 1110 42-43 -0.4294926873577225 1110 44-45 -0.4388296384511392 1110 46-47 -0.43482858494901677 1110 48-49 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-0.33132894619291875 2314 16-17 -0.40042278369391937 2314 18-19 -0.4083156986271277 2314 20-21 -0.41488484529786973 2314 22-23 -0.32542029778328896 2314 24-25 -0.1950672574374579 2314 26-27 -0.18700635177296476 2314 28-29 -0.3925691792140711 2314 30-31 -0.429143755791479 2314 32-33 -0.6483384509952117 2314 34-35 -0.7502509728959126 2314 36-37 -1.1441329186136429 2314 38-39 -0.8074194527771468 2314 40-41 -0.6600338175139839 2314 42-43 -0.4722607874662401 2314 44-45 -0.533992572825376 2314 46-47 -0.34102877453739566 2314 48-49 -0.370534570758668 2314 50-51 -0.21356034294375803 2314 52-53 -0.1272115072990978 2314 54-55 -0.2502201459182878 2314 56-57 -0.27066399457903145 2314 58-59 -0.34136010792210314 2314 60-61 -0.2461572337329372 2314 62-63 -0.22766157822178457 2314 64-65 -0.20244992621860192 2314 66-67 -0.2969708136138358 2314 68-69 -0.2968091469094176 2314 70-71 -0.11799167963263457 2314 72-73 -0.08183469787768871 2314 74-75 -0.07683493066615554 2314 76-77 -0.1362027826434442 2315 1 -0.9890281800328893 2315 2 -1.0331871217416406 2315 3 -0.8368320284354311 2315 4 -0.8640735353385693 2315 5 -1.0754712510472189 2315 6 -0.8913012919710823 2315 7 -1.3252301238699786 2315 8 -1.362220000489799 2315 9 -0.9243876560013149 2315 10-11 -1.1487282684526292 2315 12-13 -1.0298922179589525 2315 14-15 -0.9178346305904128 2315 16-17 -1.0053191901510345 2315 18-19 -0.9912862771035051 2315 20-21 -1.0053942455596783 2315 22-23 -1.2432598305517288 2315 24-25 -1.2182738700581979 2315 26-27 -1.1954572193307058 2315 28-29 -1.209600210230228 2315 30-31 -1.2983572174220015 2315 32-33 -1.2421823896463096 2315 34-35 -1.278460631171761 2315 36-37 -1.3179814298743153 2315 38-39 -1.2226784325074433 2315 40-41 -1.3054244372263852 2315 42-43 -1.4107137927602302 2315 44-45 -1.4684036883935434 2315 46-47 -1.2852955557669148 2315 48-49 -1.191601713835123 2315 50-51 -1.1890712949214546 2315 52-53 -1.2311632654427207 2315 54-55 -1.4354510709555548 2315 56-57 -1.6004698567635458 2315 58-59 -1.4445941974693142 2315 60-61 -1.432210444381667 2315 62-63 -1.2268729268774905 2315 64-65 -1.2462759953756084 2315 66-67 -1.1893708723809482 2315 68-69 -1.338324149856028 2315 70-71 -1.1534595302255042 2315 72-73 -0.9829293178806253 2315 74-75 -1.1146751319930353 2315 76-77 -1.1231415443411557 2316 1 -2.0678837665832432 2316 2 -1.628449241685587 2316 3 -1.9102487336749192 2316 4 -2.8211075108240067 2316 5 -2.1645107446077176 2316 6 -2.128370977467199 2316 7 -1.8225864634436846 2316 8 -1.661205091583092 2316 9 -1.9942351807186398 2316 10-11 -1.892558551688623 2316 12-13 -2.3615092315079167 2316 14-15 -3.6719449684245973 2316 16-17 -2.787722975430377 2316 18-19 -2.3531600832235497 2316 20-21 -2.0727733374075896 2316 22-23 -2.436169099543328 2316 24-25 -2.4325638593761383 2316 26-27 -2.3391086566745685 2316 28-29 -2.9721421795057843 2316 30-31 -3.2271977617106558 2316 32-33 -3.2671052098723976 2316 34-35 -3.0214464086109167 2316 36-37 -3.095830436516124 2316 38-39 -3.1807781101342556 2316 40-41 -2.930778135187783 2316 42-43 -3.0913503057227487 2316 44-45 -2.8641226971244294 2316 46-47 -3.0472720179270922 2316 48-49 -2.770740509079676 2316 50-51 -2.9370371760680136 2316 52-53 -2.97374692977408 2316 54-55 -3.05761972361039 2316 56-57 -2.785882003355738 2316 58-59 -2.917876546410646 2316 60-61 -2.7085306474817834 2316 62-63 -2.6986802921695983 2316 64-65 -2.6070082053410033 2316 66-67 -2.629739910718463 2316 68-69 -2.6573739340530516 2316 70-71 -2.5290006306454593 2316 72-73 -2.4051140596111367 2316 74-75 -2.661906468323899 2316 76-77 -3.0340367386912703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75313.0 3 6865.0 4 8806.0 5 10992.0 6 12333.0 7 12965.0 8 14879.0 9 15526.0 10 18453.0 11 20188.0 12 16650.0 13 14633.0 14 14761.0 15 15522.0 16 15427.0 17 16386.0 18 17557.0 19 18713.0 20 19928.0 21 21627.0 22 23764.0 23 27618.0 24 31939.0 25 37660.0 26 44229.0 27 52359.0 28 61902.0 29 73202.0 30 87158.0 31 104865.0 32 128463.0 33 162153.0 34 215285.0 35 288590.0 36 407587.0 37 545335.0 38 485520.0 39 124762.0 40 795.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.83393882616536 26.526915129697358 28.28401639569818 22.3551296484391 2 22.63896923835232 26.673599483371635 28.264274243556514 22.42315703471953 3 22.776753416321903 26.916610861900786 27.780315747952038 22.52631997382527 4 22.465121627068225 26.76134942346941 28.271986172806884 22.50154277665548 5 22.70984143991746 26.88139688702032 28.008071571439796 22.400690101622416 6 22.769750701092974 26.774275124447634 28.07604064642133 22.379933528038062 7 22.673427612277585 26.389980410708485 28.136717720327542 22.79987425668638 8 22.55144893698547 26.78650445691506 27.927024571517517 22.735022034581956 9 22.586055842072835 26.635638196210227 27.99268527253629 22.785620689180654 10-11 22.990589299513974 26.740297632936503 27.746077141872433 22.52303592567709 12-13 23.082451141465935 26.44714761573106 27.705332753317823 22.76506848948518 14-15 22.71927134342387 26.783550433471532 27.84256145242087 22.65461677068373 16-17 22.940074648702545 26.55109173803913 27.960470494132583 22.548363119125742 18-19 22.881236082263808 26.55415007495415 27.68469261153109 22.87992123125095 20-21 22.868844426159104 26.71648657288856 27.609502199974038 22.805166800978295 22-23 22.91827236876486 26.782262438014993 27.30255960827418 22.996905584945967 24-25 22.964049497308253 26.904772972199353 27.136577537097907 22.994599993394488 26-27 22.915813970342143 26.938028082446568 27.14594710793182 23.000210839279465 28-29 23.13055267665177 26.831661870418905 27.14909636735257 22.88868908557675 30-31 22.900868977079487 26.697803011783222 27.23150559043093 23.169822420706357 32-33 22.815976164361697 27.04642586205895 27.168658728588607 22.968939244990747 34-35 22.948685744042262 26.698714219600756 27.301096503935284 23.051503532421698 36-37 22.959264016859095 26.849169260894918 27.375908948168743 22.81565777407725 38-39 22.82495916862707 26.761956688929615 27.464247129419167 22.948837013024146 40-41 22.726124656722043 26.767638776641594 27.31437610329788 23.19186046333848 42-43 22.690381027329074 27.000960339257695 27.11222269840806 23.19643593500517 44-45 22.681566842542072 26.685520216701658 27.475247070595003 23.15766587016126 46-47 22.626007364763744 26.729973340802882 27.470700264145613 23.173319030287757 48-49 22.830118838975 26.695324890287957 27.270706308096393 23.20384996264065 50-51 22.63445348146787 26.888601377353893 27.194749681213388 23.282195459964854 52-53 22.739160221062065 26.73251429043298 27.27851740389703 23.249808084607928 54-55 22.68016724133001 26.753994902879626 27.29224004938399 23.27359780640637 56-57 22.613246496659762 26.89145512637528 27.28589577305734 23.20940260390762 58-59 22.75023442981608 26.8413538935379 27.22914736656536 23.179264310080658 60-61 22.636326634086316 26.7290537294473 27.150528016381305 23.48409162008508 62-63 22.69448508909295 26.506653243101447 27.36881250532322 23.430049162482376 64-65 22.593872791041814 26.532788466919488 27.353895384058347 23.51944335798035 66-67 22.570465810882425 26.643080970049184 27.448341177733383 23.33811204133501 68-69 22.629990462572135 26.4390284332179 27.483986158572698 23.44699494563727 70-71 22.481583549372882 26.282467306507773 27.56336297596321 23.67258616815614 72-73 22.636982786064067 26.13028266455519 27.52193638307406 23.710798166306688 74-75 22.453321151031677 26.1445463138083 27.471039239883126 23.931093295276902 76-77 22.327863144196545 25.997330639902895 27.82460615158379 23.850200064316766 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9014.0 1 5402.5 2 1702.0 3 1630.0 4 1647.0 5 1664.5 6 1708.0 7 1822.0 8 1972.0 9 2034.0 10 2110.5 11 2259.0 12 2404.0 13 2477.0 14 2661.0 15 3014.5 16 3482.5 17 3781.0 18 4062.5 19 4795.0 20 5935.0 21 7634.5 22 8645.0 23 10040.0 24 13364.0 25 18149.0 26 21005.0 27 24230.5 28 32520.0 29 41635.5 30 45687.0 31 52521.0 32 65931.5 33 78531.5 34 89771.5 35 94988.0 36 101721.0 37 113387.0 38 119861.5 39 121403.0 40 125477.5 41 132846.0 42 140893.5 43 145647.0 44 146842.0 45 149171.5 46 154008.0 47 159090.5 48 160471.0 49 161833.0 50 161923.0 51 155810.5 52 150970.0 53 147703.0 54 138953.0 55 124808.5 56 108828.5 57 101510.0 58 95813.0 59 81726.0 60 67435.0 61 61534.0 62 53932.0 63 39189.5 64 28313.5 65 24578.0 66 21139.0 67 15845.0 68 11787.0 69 8103.0 70 6622.0 71 5693.0 72 3919.5 73 2747.0 74 2419.0 75 2051.5 76 1427.0 77 925.5 78 681.0 79 549.5 80 341.0 81 225.5 82 155.0 83 123.0 84 112.5 85 91.5 86 73.0 87 65.0 88 61.0 89 53.0 90 45.0 91 41.0 92 37.5 93 36.0 94 32.5 95 26.5 96 26.0 97 34.0 98 41.5 99 75.0 100 109.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0029351425225715515 2 0.008377385949839639 3 0.011618272485179059 4 0.01932302160692938 5 0.014278245396259529 6 0.014033650186045233 7 0.01699936710989357 8 0.0190478519954383 9 0.018191768759688264 10-11 0.021677250505241982 12-13 0.020867028871407126 14-15 0.007735323523027111 16-17 0.005839710643866317 18-19 0.01154183648198709 20-21 0.010104839621978102 22-23 0.014171234991790772 24-25 0.021830122511625916 26-27 0.013651470170085393 28-29 0.00830094994664767 30-31 0.007811759526219078 32-33 0.00956978759963433 34-35 0.0110526460615585 36-37 0.009447489994527181 38-39 0.016907643906063207 40-41 0.012260334911991586 42-43 0.015638806253076547 44-45 0.0181000455558579 46-47 0.01663247429457213 48-49 0.01359032136753182 50-51 0.027195929935702034 52-53 0.03179737732785848 54-55 0.025208593852710875 56-57 0.015577657450522977 58-59 0.016556038291380158 60-61 0.019598191218420467 62-63 0.025636635470585898 64-65 0.020576572059277647 66-67 0.01984278642863476 68-69 0.029366712426353914 70-71 0.05581356953077467 72-73 0.06801275564021267 74-75 0.07891252969538724 76-77 0.07677232160601215 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3270710.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.01604887855155 #Duplication Level Percentage of deduplicated Percentage of total 1 71.83007649419052 35.20826540389339 2 13.569916298835052 13.302873611631044 3 5.639056744888514 8.292128431091436 4 2.8397063295959697 5.5676473700883315 5 1.637939924805578 4.014267170720063 6 1.0481698481911033 3.0826286707175448 7 0.6994286469861138 2.3998260123411375 8 0.4995077374805817 1.9587116540450311 9 0.3614257541747503 1.5944096189337227 >10 1.702850720449467 14.63217751516267 >50 0.11257763398400353 3.7979079410430177 >100 0.05671207686473597 4.874422338190954 >500 0.0021931579619247524 0.7254402947229754 >1k 3.759699357971253E-4 0.2753226976034729 >5k 6.266165596618755E-5 0.273971269815147 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8920 0.27272365938894 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.0574401276786995E-5 2 0.0 0.0 0.0 4.2804161787501796E-4 3.0574401276786995E-5 3 0.0 0.0 0.0 8.866576370268229E-4 3.0574401276786995E-5 4 0.0 0.0 0.0 0.0017427408727768588 3.0574401276786995E-5 5 0.0 0.0 0.0 0.0034854817455537176 3.0574401276786995E-5 6 0.0 0.0 0.0 0.006940389089830648 3.0574401276786995E-5 7 0.0 0.0 0.0 0.014614563810304185 3.0574401276786995E-5 8 0.0 0.0 0.0 0.022839077753759887 9.172320383036099E-5 9 0.0 0.0 0.0 0.03133876130870667 1.2229760510714798E-4 10 0.0 0.0 0.0 0.04262071537984107 1.52872006383935E-4 11 0.0 0.0 0.0 0.054024967056082625 1.52872006383935E-4 12 0.0 0.0 0.0 0.07081031335703869 1.52872006383935E-4 13 0.0 0.0 0.0 0.09181492703419135 1.52872006383935E-4 14 0.0 0.0 0.0 0.11688593608115669 1.52872006383935E-4 15 0.0 0.0 0.0 0.1460233404979347 1.52872006383935E-4 16 0.0 0.0 0.0 0.1744881080866234 1.52872006383935E-4 17 0.0 0.0 0.0 0.20182162282807098 1.8344640766072197E-4 18 0.0 0.0 0.0 0.23108132484995614 1.8344640766072197E-4 19 0.0 0.0 0.0 0.2624812349612164 2.1402080893750898E-4 20 0.0 0.0 0.0 0.2974277756205839 2.1402080893750898E-4 21 0.0 0.0 0.0 0.3354929052101837 2.1402080893750898E-4 22 0.0 0.0 0.0 0.3760039869019265 2.1402080893750898E-4 23 0.0 0.0 0.0 0.4167290894026068 2.1402080893750898E-4 24 0.0 0.0 0.0 0.45727074549562635 2.4459521021429596E-4 25 0.0 0.0 0.0 0.5003500768946192 2.4459521021429596E-4 26 0.0 0.0 0.0 0.5470983364468265 2.4459521021429596E-4 27 0.0 0.0 0.0 0.5930516615658374 2.4459521021429596E-4 28 0.0 0.0 0.0 0.6400139419269822 2.4459521021429596E-4 29 0.0 0.0 0.0 0.6853252046191806 3.3631841404465696E-4 30 0.0 0.0 0.0 0.7399922341020757 3.3631841404465696E-4 31 0.0 0.0 0.0 0.7977778525152032 3.6689281532144394E-4 32 0.0 0.0 0.0 0.8603025031262326 3.6689281532144394E-4 33 0.0 0.0 0.0 0.9266183794955836 3.6689281532144394E-4 34 0.0 0.0 0.0 0.9921698958330148 3.6689281532144394E-4 35 0.0 0.0 0.0 1.0631636555977142 3.6689281532144394E-4 36 0.0 0.0 0.0 1.1337905225470921 3.6689281532144394E-4 37 0.0 0.0 0.0 1.2103793977454436 3.6689281532144394E-4 38 0.0 0.0 0.0 1.2870905705489022 3.97467216598231E-4 39 0.0 0.0 0.0 1.3678987131234503 3.97467216598231E-4 40 0.0 0.0 0.0 1.4525286558575967 3.97467216598231E-4 41 0.0 0.0 0.0 1.5455971333441363 4.2804161787501796E-4 42 0.0 0.0 0.0 1.6460646159396584 4.2804161787501796E-4 43 0.0 0.0 0.0 1.7538393804403325 4.2804161787501796E-4 44 0.0 0.0 0.0 1.8648550314763461 4.2804161787501796E-4 45 0.0 0.0 0.0 1.9807931611179224 4.2804161787501796E-4 46 0.0 0.0 0.0 2.10813554243574 4.5861601915180494E-4 47 0.0 0.0 0.0 2.2352944773458976 4.5861601915180494E-4 48 0.0 0.0 0.0 2.3666726796322513 4.891904204285919E-4 49 0.0 0.0 0.0 2.50275933971523 4.891904204285919E-4 50 0.0 0.0 0.0 2.6417811423207804 4.891904204285919E-4 51 0.0 0.0 0.0 2.793002131035769 4.891904204285919E-4 52 0.0 0.0 0.0 2.9551076066052935 4.891904204285919E-4 53 0.0 0.0 0.0 3.127577804207649 4.891904204285919E-4 54 0.0 0.0 0.0 3.307447006918987 4.891904204285919E-4 55 0.0 0.0 0.0 3.4914131794014143 4.891904204285919E-4 56 0.0 0.0 0.0 3.6933570998345924 4.891904204285919E-4 57 0.0 3.0574401276786995E-5 0.0 3.8968908891341636 5.50339222982166E-4 58 0.0 3.0574401276786995E-5 0.0 4.112899034154664 6.1148802553574E-4 59 0.0 3.0574401276786995E-5 0.0 4.3281428191432445 6.1148802553574E-4 60 0.0 3.0574401276786995E-5 0.0 4.545129345004601 6.1148802553574E-4 61 0.0 3.0574401276786995E-5 0.0 4.780399362829478 6.1148802553574E-4 62 0.0 3.0574401276786995E-5 0.0 5.024077341005469 6.1148802553574E-4 63 0.0 3.0574401276786995E-5 0.0 5.2813303533483555 6.1148802553574E-4 64 0.0 3.0574401276786995E-5 0.0 5.550354510182804 6.1148802553574E-4 65 0.0 3.0574401276786995E-5 0.0 5.84062787590462 6.1148802553574E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 350 0.0 18.252161 4 TCGCGCT 550 0.0 18.070005 37 ATTCGCG 440 0.0 17.750586 56 TGCGTAA 475 0.0 17.186886 37 CGCTCCG 550 0.0 16.781088 25 TTGCGTA 490 0.0 16.659737 36 CTCGCGC 555 0.0 16.62711 36 CAATTCG 515 0.0 16.543728 54 GCGTAAG 525 0.0 16.226871 38 CGCGGAA 475 0.0 15.695974 59 CGCGCTT 635 0.0 15.651904 38 CGATCGT 590 0.0 15.639561 1 AATTCGC 555 0.0 15.351857 55 CCTCGCG 630 0.0 15.209436 35 CGTAAGT 555 0.0 14.710397 39 TCGCGGA 565 0.0 14.452909 58 CGACTTA 295 1.0884105E-7 14.449331 69 GATCGTT 655 0.0 14.087759 2 TAAGGCG 950 0.0 13.825292 17 ATCGTTC 705 0.0 13.088627 3 >>END_MODULE