FastQCFastQC Report
Thu 26 May 2016
SRR936124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936124_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3270710
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80020.24465635901684954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57270.17509959611215914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41630.12728123251526427No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14000.046.34585100-101
GGTATCA85250.039.30371
CGTACAT20100.036.278763100-101
CGGTATC10850.032.64372100-101
GTATCAA165150.032.4687541
TACGCAG10050.030.503775100-101
GCGTATC3850.029.376854100-101
TACCGTA3150.029.28759698-99
CCTGTCG10750.028.78840496-97
ACCGTAT4000.028.27197898-99
ACGTATC6550.028.172981100-101
AGTATCA33300.027.886421100-101
TATACGT4200.027.63173196-97
CCGTACA10250.027.585583100-101
TCGTATC7750.027.267107100-101
AGGTATC23400.026.456348100-101
CGAGTAC15200.025.651342100-101
CGTCCTA3750.025.398094100-101
TCGGTAT4850.025.160807100-101
TCGTACA12000.025.050856100-101
Produced by FastQC (version 0.11.5)