FastQCFastQC Report
Thu 26 May 2016
SRR936123_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936123_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815371
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56440.31090063683952207No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24130.13292048842908696No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT200.00657492753.2468860
CGGTTAC2450.023.1784824
ATTCGCG2301.0913936E-1121.60683456
TTGCGTA1601.0378899E-619.96537836
CGTTACA900.004864049619.72106627
CGCGGAA2809.094947E-1219.01674359
AATTCGC2851.0913936E-1118.68105555
CTCGCGC3101.8189894E-1218.31948936
TCGCGCT2951.8189894E-1118.04879437
CGATCGT3305.456968E-1217.2082671
CCTCGCG3551.8189894E-1216.99666235
TCGCGGA3206.366463E-1116.63873358
ATCGTTC3004.5474735E-1016.56253
CGTAAGT1956.861428E-616.38230139
GCGTAAG1956.861428E-616.38230138
CAATTCG2854.098183E-916.19381954
CGCTCCG3502.4738256E-1015.21339425
CGCGCTT4051.4733814E-1014.02271138
AACTCGA3554.9149094E-913.99763713
TGACACG3605.980837E-913.80360615
Produced by FastQC (version 0.11.5)