##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936123_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1815371 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.86213561855951 40.0 36.0 41.0 30.0 41.0 2 36.721385876495766 39.0 36.0 41.0 30.0 41.0 3 36.631656559458094 39.0 36.0 41.0 30.0 41.0 4 36.5092061071814 39.0 36.0 41.0 30.0 41.0 5 36.43834676217699 39.0 36.0 41.0 30.0 41.0 6 36.34906804173912 39.0 36.0 41.0 30.0 41.0 7 36.174292197021984 39.0 35.0 40.0 29.0 41.0 8 35.9960289108948 39.0 35.0 40.0 29.0 41.0 9 35.89685083655077 39.0 35.0 40.0 29.0 41.0 10-11 36.09253012194202 39.0 35.0 40.5 29.5 41.0 12-13 36.27359421297355 39.0 35.0 41.0 30.0 41.0 14-15 36.21666755721007 39.0 35.0 41.0 29.5 41.0 16-17 36.048826107721226 39.0 35.0 41.0 29.0 41.0 18-19 35.886730315731604 39.0 35.0 41.0 28.0 41.0 20-21 35.67616729583099 39.0 35.0 41.0 27.5 41.0 22-23 35.41517932147203 39.0 35.0 41.0 26.5 41.0 24-25 35.17887638394576 39.0 35.0 41.0 26.0 41.0 26-27 34.217433240918794 38.0 33.5 40.0 24.0 40.5 28-29 34.23441847424026 38.0 33.5 40.0 24.0 40.5 30-31 34.60691974257604 38.0 34.0 40.0 24.0 41.0 32-33 34.44494513793599 38.0 34.0 40.0 23.5 41.0 34-35 34.18259297961684 38.0 34.0 40.0 23.0 41.0 36-37 33.889353470998486 37.5 33.0 40.0 22.0 41.0 38-39 33.60646418831192 37.0 33.0 40.0 22.0 41.0 40-41 33.22619591257104 36.5 33.0 40.0 20.5 41.0 42-43 32.86641601083195 36.0 32.0 39.0 19.5 41.0 44-45 32.50458418692377 35.5 32.0 39.0 18.0 41.0 46-47 32.116198837592975 35.0 32.0 38.5 16.5 40.0 48-49 31.65977147370978 35.0 31.0 37.5 12.0 40.0 50-51 31.226460872185356 35.0 31.0 37.0 7.5 39.0 52-53 30.773242218808168 35.0 31.0 36.5 6.5 39.0 54-55 30.31881031480617 35.0 30.0 36.0 2.0 38.5 56-57 29.881442966754456 34.0 30.0 35.5 2.0 37.0 58-59 29.456915693816857 34.0 29.5 35.0 2.0 37.0 60-61 29.121695785599748 34.0 29.0 35.0 2.0 36.5 62-63 28.786474500253668 34.0 29.0 35.0 2.0 36.0 64-65 28.428605778102657 34.0 29.0 35.0 2.0 36.0 66-67 28.059981403250355 34.0 28.5 35.0 2.0 35.0 68-69 27.721985753876204 34.0 27.5 35.0 2.0 35.0 70-71 27.316443580953976 34.0 27.0 35.0 2.0 35.0 72-73 26.849457769238356 33.0 25.5 35.0 2.0 35.0 74-75 26.287867053070695 33.0 25.0 35.0 2.0 35.0 76-77 24.595554572591496 31.0 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7848023646796136 1101 2 0.5718351751295572 1101 3 0.7704858808587645 1101 4 0.758950098423739 1101 5 0.7950531095404685 1101 6 0.5676421763011277 1101 7 0.7106190987357408 1101 8 0.9013740805049437 1101 9 0.8753365596065166 1101 10-11 0.6987779427743561 1101 12-13 0.812768657339511 1101 14-15 0.9104660525727439 1101 16-17 0.8591966779201456 1101 18-19 0.9344108473111774 1101 20-21 0.9754616086840642 1101 22-23 1.008337620101706 1101 24-25 0.982762431259026 1101 26-27 0.8831222751011083 1101 28-29 0.8739801733039201 1101 30-31 0.8988603257811079 1101 32-33 0.8961458877954414 1101 34-35 0.9102368985474172 1101 36-37 0.977726925359697 1101 38-39 0.8543705728992279 1101 40-41 0.898151742834429 1101 42-43 0.8084316088195536 1101 44-45 0.7707027453577595 1101 46-47 0.8124710035839584 1101 48-49 0.9208175813235933 1101 50-51 0.8343797771434645 1101 52-53 0.7346332147287171 1101 54-55 0.7333628761929418 1101 56-57 0.6986173929519097 1101 58-59 0.6873590420235729 1101 60-61 0.7632798574892448 1101 62-63 0.6932663232383973 1101 64-65 0.5046535259302267 1101 66-67 0.5557378763574405 1101 68-69 0.6938585379891791 1101 70-71 0.6224064784698626 1101 72-73 0.4020559632497793 1101 74-75 0.5023913922011012 1101 76-77 0.4840044856467465 1102 1 0.7893461206937786 1102 2 0.6816605537920282 1102 3 0.7545041271331314 1102 4 0.5848379682525291 1102 5 0.7552478087499424 1102 6 0.6404944538907742 1102 7 0.5100591642550398 1102 8 0.6734648704346426 1102 9 0.5501671949153035 1102 10-11 0.4155190543953182 1102 12-13 0.6624097920006591 1102 14-15 0.568900210899443 1102 16-17 0.5709721465167661 1102 18-19 0.7228791399563264 1102 20-21 0.7444251296883877 1102 22-23 0.7595065704568995 1102 24-25 0.7323671663066236 1102 26-27 0.56423590966061 1102 28-29 0.751765760218646 1102 30-31 0.790286280641709 1102 32-33 0.7839239430879985 1102 34-35 0.8868710572613523 1102 36-37 0.8872173501315714 1102 38-39 0.8686620679746326 1102 40-41 0.77126675593248 1102 42-43 0.7935848260226308 1102 44-45 0.7366615557099649 1102 46-47 0.6863823550357822 1102 48-49 0.7673279619899809 1102 50-51 0.9559498396230133 1102 52-53 0.7867280318497016 1102 54-55 0.6949150099266674 1102 56-57 0.7726238093518738 1102 58-59 0.7090073249492512 1102 60-61 0.6963796911739912 1102 62-63 0.7028926657161669 1102 64-65 0.6819022185451722 1102 66-67 0.687757338533789 1102 68-69 0.6557707955590395 1102 70-71 0.5839591930484893 1102 72-73 0.6456022924068421 1102 74-75 0.7343337956211151 1102 76-77 0.9022714768120963 1103 1 0.483058430995186 1103 2 0.19977191720023058 1103 3 0.4811596000854408 1103 4 0.5339980181874253 1103 5 0.5247395471909968 1103 6 0.5398567800171676 1103 7 0.6497504353802412 1103 8 0.6129954394671842 1103 9 0.45450461873124226 1103 10-11 0.5003376878680967 1103 12-13 0.5178756173250747 1103 14-15 0.6025359558881433 1103 16-17 0.7362303389132663 1103 18-19 0.7496040052524151 1103 20-21 0.8446754172585358 1103 22-23 0.8792106624977336 1103 24-25 0.6062116171032059 1103 26-27 0.4851569924417234 1103 28-29 0.4431955183838312 1103 30-31 0.6070406807906892 1103 32-33 0.5655961261458415 1103 34-35 0.5865430242105205 1103 36-37 0.5983020777618364 1103 38-39 0.5507200280159807 1103 40-41 0.6612243311930968 1103 42-43 0.6236128588435577 1103 44-45 0.49764037476032996 1103 46-47 0.19997057144180275 1103 48-49 0.3409930502458529 1103 50-51 0.49241089906132274 1103 52-53 0.4299090366631013 1103 54-55 0.6729182883413216 1103 56-57 0.6777785378021193 1103 58-59 0.6039196898142727 1103 60-61 0.7558938757967084 1103 62-63 0.5397227575470289 1103 64-65 0.3137292794901221 1103 66-67 0.47437664776768074 1103 68-69 0.35780520588505027 1103 70-71 0.5466283344916185 1103 72-73 0.45457179885906385 1103 74-75 0.4601319225259566 1103 76-77 0.6056686362857384 1104 1 0.5905305994987131 1104 2 0.5071199014418823 1104 3 0.3388527931164589 1104 4 0.31955144454411055 1104 5 0.46916159187680506 1104 6 0.23602612805711942 1104 7 0.4322344431743801 1104 8 0.5676410598093611 1104 9 0.43029968390965223 1104 10-11 0.46523254177198226 1104 12-13 0.6473538381619335 1104 14-15 0.5150117091675881 1104 16-17 0.5745650089012884 1104 18-19 0.6650443462239863 1104 20-21 0.4060795497940788 1104 22-23 0.6049599099880254 1104 24-25 0.6542516463678751 1104 26-27 0.6118249395600444 1104 28-29 0.41210290214252865 1104 30-31 0.5300933557372858 1104 32-33 0.6255582844008742 1104 34-35 0.781244006981602 1104 36-37 0.6017603968611382 1104 38-39 0.554967510975473 1104 40-41 0.5749261658066374 1104 42-43 0.6493334427881621 1104 44-45 0.645856563872286 1104 46-47 0.34360939023981274 1104 48-49 0.5653197501553855 1104 50-51 0.5846294428359116 1104 52-53 0.6136735205506696 1104 54-55 0.6046587300384942 1104 56-57 0.6744463089805208 1104 58-59 0.46241414257513824 1104 60-61 0.3158270772728464 1104 62-63 0.54212726981957 1104 64-65 0.5380768722654388 1104 66-67 0.6058697472573122 1104 68-69 0.4457454772137339 1104 70-71 0.4408741460218444 1104 72-73 0.557924773929841 1104 74-75 0.6616571750363995 1104 76-77 0.5739052490903553 1105 1 0.3095070733411802 1105 2 0.6557402677875146 1105 3 0.52430278611142 1105 4 0.4723457370530255 1105 5 0.349080836338338 1105 6 0.2031354893052395 1105 7 0.3020017680753213 1105 8 0.5881873810856462 1105 9 0.41070281498293326 1105 10-11 0.5439172826393843 1105 12-13 0.5122594856611329 1105 14-15 0.27195065036476507 1105 16-17 0.40728375474765954 1105 18-19 0.5293163070244944 1105 20-21 0.678651225722966 1105 22-23 0.7606853627111931 1105 24-25 0.8259196721320592 1105 26-27 0.5460515651783808 1105 28-29 0.5884590958552351 1105 30-31 0.6544823905146089 1105 32-33 0.7589471790774027 1105 34-35 0.7413876641872719 1105 36-37 0.7856581448305704 1105 38-39 0.7464779413004692 1105 40-41 0.7877443113749578 1105 42-43 0.8144099054944078 1105 44-45 0.726048106813181 1105 46-47 0.6916504936593455 1105 48-49 0.4466121620804877 1105 50-51 0.7138830288775608 1105 52-53 0.5940030088953598 1105 54-55 0.5127993050265509 1105 56-57 0.6485896285073416 1105 58-59 0.6502902144360689 1105 60-61 0.5058601626157895 1105 62-63 0.2520940048602185 1105 64-65 0.416988468660179 1105 66-67 0.47612544918028377 1105 68-69 0.31211248383439383 1105 70-71 0.2261702077524106 1105 72-73 0.04726777302098384 1105 74-75 -0.013202813971076921 1105 76-77 0.23091980879664575 1106 1 -0.09411830430232726 1106 2 0.08957712579355359 1106 3 -0.2841564283900908 1106 4 0.03850887904698652 1106 5 -0.42795843858613836 1106 6 -0.035535205558808514 1106 7 0.06393530281852122 1106 8 0.22323269830618386 1106 9 -0.041260931240628906 1106 10-11 -0.39112803458115053 1106 12-13 -0.24151694938419155 1106 14-15 -0.10599496897057747 1106 16-17 -0.1262509884547569 1106 18-19 -0.06494351170662327 1106 20-21 -0.07935481657310817 1106 22-23 -0.04052309650331409 1106 24-25 0.08030033678462445 1106 26-27 -0.024039069262705937 1106 28-29 -0.07649558692422431 1106 30-31 -0.14219434664550334 1106 32-33 -0.055252216692984746 1106 34-35 -0.07220750197587478 1106 36-37 -0.024613758492684212 1106 38-39 -0.23418671126750468 1106 40-41 -0.17207441974286297 1106 42-43 -0.02003118211888477 1106 44-45 -0.3491784792895416 1106 46-47 -0.1536667268844596 1106 48-49 -0.388131040336436 1106 50-51 -0.47765775662092835 1106 52-53 -0.29240182494149636 1106 54-55 -0.18538800615169748 1106 56-57 -0.40397834732347704 1106 58-59 -0.4234257976485232 1106 60-61 -0.20954769236910664 1106 62-63 -0.2823470827530734 1106 64-65 -0.40023207514646586 1106 66-67 -0.4676811973453958 1106 68-69 -0.7525400841414331 1106 70-71 -0.7762467106765918 1106 72-73 -0.7769014112690442 1106 74-75 -0.9216620284725892 1106 76-77 -0.666059043167099 1107 1 -0.10220621608687708 1107 2 -0.17180859941137783 1107 3 -0.044239660932220204 1107 4 -0.034793040788208884 1107 5 -0.13938755720803897 1107 6 -0.025159089019446412 1107 7 -0.14665338107553083 1107 8 -0.06736723509397535 1107 9 -0.11935734917013718 1107 10-11 -0.005459422256038238 1107 12-13 0.043722042232175795 1107 14-15 -0.01623270968326551 1107 16-17 0.06765219332655903 1107 18-19 0.16317162664332585 1107 20-21 -0.0019375099326168765 1107 22-23 0.17554247500438436 1107 24-25 -0.17448389527267238 1107 26-27 -0.2881900680014482 1107 28-29 -0.09887485746621394 1107 30-31 0.27913399227519164 1107 32-33 0.2504425869668978 1107 34-35 0.27931696138645634 1107 36-37 0.1601988906452263 1107 38-39 0.07985205121780581 1107 40-41 0.12030196940722959 1107 42-43 0.12314932241801557 1107 44-45 0.10454740199366341 1107 46-47 0.050622233588704546 1107 48-49 0.24151140826005246 1107 50-51 0.04290485671525701 1107 52-53 0.08212063179880147 1107 54-55 0.08759730565114054 1107 56-57 0.11154476333750196 1107 58-59 -0.06357117853574934 1107 60-61 0.028872994299366184 1107 62-63 -0.0248429547709641 1107 64-65 0.06921654376960973 1107 66-67 0.02250257334411998 1107 68-69 -0.16113060039369387 1107 70-71 -0.02148352830863942 1107 72-73 -0.246653007680937 1107 74-75 -0.3997334283286911 1107 76-77 -0.4132296716038475 1108 1 -0.008375733424827558 1108 2 0.04570391159927567 1108 3 -0.01862653267943415 1108 4 -0.10731527989612744 1108 5 0.023766346775808245 1108 6 -0.014252327104543383 1108 7 -0.05133770996588538 1108 8 -0.09233730869195256 1108 9 -0.07545281681251481 1108 10-11 -0.22219465345564515 1108 12-13 -0.18787023594946817 1108 14-15 -0.08976099214978461 1108 16-17 -0.1415504576202764 1108 18-19 -0.030772108494304007 1108 20-21 -0.1357982255204817 1108 22-23 0.00997445414785858 1108 24-25 -0.011896737651689193 1108 26-27 -0.21109336458419392 1108 28-29 -0.04552455239437592 1108 30-31 -0.05230921854541748 1108 32-33 0.01390817725106075 1108 34-35 5.927779497127972E-4 1108 36-37 0.06770791696433065 1108 38-39 -0.0022427646581917315 1108 40-41 0.13338327565030994 1108 42-43 0.04083300276855084 1108 44-45 -0.010077654874571351 1108 46-47 -0.05923024780206987 1108 48-49 -0.07108801031271028 1108 50-51 -0.036096488762872525 1108 52-53 -0.054011909089858534 1108 54-55 -0.055878900036006485 1108 56-57 -0.12283833332719851 1108 58-59 -0.20447790818498746 1108 60-61 -0.2894024383025027 1108 62-63 -0.14739052242399353 1108 64-65 -0.16287907874794882 1108 66-67 -0.18491368919512396 1108 68-69 -0.20532568504265925 1108 70-71 -0.2548784890574609 1108 72-73 -0.05233708812568594 1108 74-75 -0.1006129902900561 1108 76-77 -0.3320813689390256 1109 1 0.1284826373318495 1109 2 0.05878035179436125 1109 3 -0.2513369484407022 1109 4 0.0700665347738223 1109 5 6.073337862844141E-4 1109 6 0.2908469051133338 1109 7 0.08908915757962887 1109 8 0.0785980334298273 1109 9 -0.15480961820321681 1109 10-11 -0.2609845515675744 1109 12-13 -0.1168769480070253 1109 14-15 -0.038382364499973676 1109 16-17 0.09124929128117287 1109 18-19 0.020282988323501172 1109 20-21 0.10841685005052426 1109 22-23 0.13449519392283804 1109 24-25 0.13049787338699304 1109 26-27 -0.06762192704496073 1109 28-29 0.10240629892630437 1109 30-31 0.1513751698847372 1109 32-33 0.2476036719635033 1109 34-35 0.28582866572796917 1109 36-37 0.14774316733856097 1109 38-39 0.1192213330770926 1109 40-41 0.15930678716704705 1109 42-43 0.20711094955497344 1109 44-45 0.0520644089624156 1109 46-47 -0.17235739059022848 1109 48-49 0.04877774853610006 1109 50-51 0.11058128418608604 1109 52-53 0.10487311674431865 1109 54-55 0.09626992414671776 1109 56-57 -0.0016036044653482406 1109 58-59 0.09587942437573105 1109 60-61 0.09713240162216508 1109 62-63 0.13718623164428934 1109 64-65 0.19425067160214127 1109 66-67 0.3151249413519821 1109 68-69 0.14037183777350748 1109 70-71 0.035048595784878955 1109 72-73 0.016572564682270752 1109 74-75 -0.005258313579449947 1109 76-77 0.15490737991412473 1110 1 -0.6393595887628081 1110 2 -0.7972924499095058 1110 3 -0.7989512543074326 1110 4 -0.5260361399696265 1110 5 -0.5270390579377775 1110 6 -0.7033112560351213 1110 7 -0.559814114624615 1110 8 -0.5882124663903809 1110 9 -0.7034362881891099 1110 10-11 -0.5750387102819303 1110 12-13 -0.5014743534782156 1110 14-15 -0.6111467270701567 1110 16-17 -0.6376431934365527 1110 18-19 -0.5814029021973397 1110 20-21 -0.5676459939734855 1110 22-23 -0.7979488632784992 1110 24-25 -0.6702323564694481 1110 26-27 -0.7124079752290697 1110 28-29 -0.5497124740662542 1110 30-31 -0.6411496551643623 1110 32-33 -0.5010644883551407 1110 34-35 -0.4519953046202758 1110 36-37 -0.3269989010928285 1110 38-39 -0.34374716193023147 1110 40-41 -0.3205413582150811 1110 42-43 -0.4681635781467435 1110 44-45 -0.39175629747965246 1110 46-47 -0.44336532907389525 1110 48-49 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74-75 -0.6066260713787379 2313 76-77 -0.7004980414663997 2314 1 -0.3432665564512476 2314 2 -0.37344404992156655 2314 3 -0.2623889391537091 2314 4 -0.3123542171384557 2314 5 -0.41074273858073695 2314 6 -0.379819589281972 2314 7 -0.37860904910162674 2314 8 -0.32927424562888774 2314 9 -0.29417323214310187 2314 10-11 -0.335845592530319 2314 12-13 -0.46288932283296447 2314 14-15 -0.46801721447380373 2314 16-17 -0.8221352797305173 2314 18-19 -0.4255193981568439 2314 20-21 -0.3104279399807055 2314 22-23 -0.20563098179656691 2314 24-25 -0.09626839744706928 2314 26-27 -0.08503595123864471 2314 28-29 -0.2929516404994317 2314 30-31 -0.4482627992447661 2314 32-33 -0.5539953194659901 2314 34-35 -0.5542934642246635 2314 36-37 -0.5745046345702605 2314 38-39 -0.6003798848292092 2314 40-41 -0.34604106394352385 2314 42-43 -0.2737417414672123 2314 44-45 -0.1742553919336629 2314 46-47 -0.25448906594224496 2314 48-49 -0.3312040585643494 2314 50-51 -0.11610106961490985 2314 52-53 -0.0028514265734465027 2314 54-55 -0.0847306331250266 2314 56-57 0.10621190335016095 2314 58-59 0.049927344525347195 2314 60-61 0.006833628272545411 2314 62-63 0.053266066368191645 2314 64-65 0.08111237032824548 2314 66-67 -0.0859978241775643 2314 68-69 0.22859552430227836 2314 70-71 0.33976191405751877 2314 72-73 0.24362412732705252 2314 74-75 0.25272339694463497 2314 76-77 0.12282014274214603 2315 1 -1.172258420552069 2315 2 -1.1637077421223054 2315 3 -1.077469546001872 2315 4 -0.95251511637926 2315 5 -0.8237719905930376 2315 6 -1.0945978089028117 2315 7 -1.1023696481648884 2315 8 -1.2259405496801818 2315 9 -1.201869700977582 2315 10-11 -1.1834364383051792 2315 12-13 -1.064029266587923 2315 14-15 -1.4352286399176748 2315 16-17 -1.3402528045066404 2315 18-19 -1.0920663025972175 2315 20-21 -1.259982475491661 2315 22-23 -1.31795024423689 2315 24-25 -1.3871559014096349 2315 26-27 -1.3293171101687165 2315 28-29 -1.260561553327264 2315 30-31 -1.398425954916398 2315 32-33 -1.5011610553726555 2315 34-35 -1.437884261556384 2315 36-37 -1.72010221479254 2315 38-39 -1.519324512611611 2315 40-41 -1.4562693034090444 2315 42-43 -1.4498648788494322 2315 44-45 -1.458311987128269 2315 46-47 -1.3647119936591174 2315 48-49 -1.2248344857904208 2315 50-51 -1.2951386572481418 2315 52-53 -1.3960336484481566 2315 54-55 -1.341985206965557 2315 56-57 -1.4903257289017589 2315 58-59 -1.3230792624400998 2315 60-61 -1.5241673303918546 2315 62-63 -1.5398249846526433 2315 64-65 -1.452625732417122 2315 66-67 -1.36308837971848 2315 68-69 -1.3407577469275047 2315 70-71 -1.4466782752277076 2315 72-73 -2.7548097567000482 2315 74-75 -3.269973492580341 2315 76-77 -2.910723550002519 2316 1 -3.0778742554027545 2316 2 -3.053279838903599 2316 3 -2.563231314824229 2316 4 -2.327447864598163 2316 5 -2.8995496700858894 2316 6 -2.6938346328189766 2316 7 -2.9091449898805237 2316 8 -2.7418239365277444 2316 9 -2.2903974565774305 2316 10-11 -2.3792358106857137 2316 12-13 -2.5742706837289333 2316 14-15 -2.8280470112853635 2316 16-17 -3.981597859274956 2316 18-19 -3.6846621926278686 2316 20-21 -2.9922438010745296 2316 22-23 -3.1054949271818373 2316 24-25 -3.3003944025517242 2316 26-27 -2.9122670365507517 2316 28-29 -2.876808769917247 2316 30-31 -2.952680910487139 2316 32-33 -3.209785653537274 2316 34-35 -3.3279400446351666 2316 36-37 -3.195229586315307 2316 38-39 -3.247752726002229 2316 40-41 -3.211122680166447 2316 42-43 -3.4843782057112733 2316 44-45 -3.222546389022998 2316 46-47 -2.8948199698639527 2316 48-49 -3.069994798719417 2316 50-51 -3.516900567753968 2316 52-53 -4.478699250574177 2316 54-55 -3.724680390302048 2316 56-57 -3.675103467748496 2316 58-59 -3.6767295742394595 2316 60-61 -3.600356418467271 2316 62-63 -3.552571470806914 2316 64-65 -3.4243560513389504 2316 66-67 -3.3990309864262365 2316 68-69 -3.4707181042761874 2316 70-71 -3.25426279228812 2316 72-73 -3.2173464460169257 2316 74-75 -3.3635085142445114 2316 76-77 -3.263841352147299 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38225.0 3 3613.0 4 4860.0 5 5608.0 6 6632.0 7 7123.0 8 8248.0 9 8739.0 10 10573.0 11 11837.0 12 9582.0 13 7977.0 14 8126.0 15 8358.0 16 8485.0 17 8763.0 18 9244.0 19 10083.0 20 10590.0 21 11416.0 22 12798.0 23 14673.0 24 17080.0 25 20005.0 26 23908.0 27 28319.0 28 33513.0 29 39945.0 30 47611.0 31 57402.0 32 70788.0 33 88771.0 34 119146.0 35 160329.0 36 225433.0 37 304524.0 38 278528.0 39 73964.0 40 552.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.649512143927872 26.76220135997007 28.738085938253192 21.85020055784886 2 22.318265708068203 27.054072666170843 28.543095611932902 22.08456601382806 3 22.33915899183034 27.0359549806598 28.38136751562284 22.24351851188702 4 22.11499313818338 27.082882260482382 28.65661001467123 22.14551458666301 5 22.29744656540487 27.06197735409988 28.418274281703624 22.222301798791623 6 22.59703714232041 26.839859035360707 28.506996722687827 22.056107099631063 7 22.419161834998246 26.674721283802395 28.491637963140487 22.41447891805887 8 22.405911808797303 27.08862809828112 28.182299503217216 22.32316058970436 9 22.297903333862237 26.893588794273725 28.328628285820066 22.479879586043968 10-11 22.823100120411265 26.929589849641562 28.022216718791622 22.22509331115555 12-13 22.820212914240948 26.768872407369965 27.981589964613462 22.429324713775628 14-15 22.485605767003346 26.800556515492445 28.230049908454802 22.48378780904941 16-17 22.890100776280622 26.652788018896484 28.15634798019125 22.30076322463164 18-19 22.88385856596863 26.739096778743352 27.84739374459033 22.529650910697686 20-21 22.78692688592569 26.810550924525504 27.82025873159916 22.58226345794964 22-23 22.74020801342605 27.003012156063516 27.482659578981355 22.77412025152908 24-25 22.874546011512795 26.989780851382683 27.407957139960683 22.72771599714384 26-27 22.723782703693594 27.099473335781564 27.34904445859265 22.827699501932187 28-29 22.972964411722334 26.931716484819873 27.317372536694617 22.777946566763173 30-31 22.880139752117383 26.84088487638372 27.373246350017133 22.905729021481765 32-33 22.632081141874327 27.20474043778313 27.398934877927445 22.764243542415098 34-35 22.732398758013087 26.9008559043961 27.458714292325286 22.90803104526553 36-37 22.697320578591075 26.848974860729147 27.702036395529227 22.751668165150555 38-39 22.5168135108594 26.93081634536322 27.664867440806663 22.887502702970718 40-41 22.59645711043227 26.928412255500128 27.49303789971821 22.98209273434939 42-43 22.399415796522657 27.112572660445228 27.31159520494777 23.176416338084348 44-45 22.4150649845768 26.839503438609945 27.643140437314717 23.102291139498536 46-47 22.48340646253303 26.732365862534735 27.5485092194584 23.235718455473837 48-49 22.54785713852798 26.741896257327408 27.543578743678143 23.166667860466475 50-51 22.404004212292023 26.975889532586578 27.37733573080566 23.242770524315738 52-53 22.53579533368382 26.763700649501658 27.40190519017184 23.29859882664268 54-55 22.508345097468755 26.754591869907944 27.4996445963772 23.2374184362461 56-57 22.355694068753564 26.898833299035612 27.452748349232873 23.292724282977943 58-59 22.416471335568865 26.889661367636315 27.44225393859942 23.251613358195407 60-61 22.410451718652073 26.602378771496067 27.41824092120578 23.568928588646084 62-63 22.489054577792665 26.53698742750345 27.514664606697618 23.459293388006262 64-65 22.423312917238782 26.566415487727202 27.501724605324345 23.50854698970967 66-67 22.371933347126827 26.55838683362486 27.623699938094585 23.44597988115373 68-69 22.38187598111195 26.276289391550083 27.73885400337763 23.602980623960335 70-71 22.28722589236208 26.15441300482489 27.784287637060707 23.77407346575232 72-73 22.397872713459176 26.027227327009108 27.672147344254995 23.902752615276725 74-75 22.238162055172527 26.01105986718235 27.747999327366003 24.002778750279116 76-77 22.184334119582683 25.927509574488028 27.918550963403565 23.969605342525725 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5967.0 1 3572.5 2 1102.0 3 1078.0 4 1130.0 5 1140.0 6 1178.5 7 1258.5 8 1276.0 9 1242.0 10 1341.0 11 1480.0 12 1607.5 13 1695.0 14 1780.0 15 2020.0 16 2358.0 17 2541.0 18 2788.0 19 3313.5 20 3964.5 21 4867.0 22 5397.0 23 6141.5 24 7862.5 25 10172.0 26 11505.0 27 13494.0 28 18117.5 29 22595.0 30 24438.0 31 27914.0 32 35089.5 33 42110.0 34 48839.0 35 52247.0 36 55853.0 37 62713.0 38 67488.5 39 69010.0 40 71226.5 41 75826.5 42 81522.0 43 84834.0 44 86336.5 45 89104.0 46 91353.0 47 91938.5 48 91540.0 49 90336.5 50 87900.5 51 84426.5 52 82185.0 53 80327.5 54 74264.0 55 65878.0 56 57116.0 57 52534.0 58 49475.0 59 42387.0 60 35104.0 61 31850.0 62 27981.5 63 20794.5 64 15091.0 65 12706.0 66 10976.0 67 8197.5 68 6050.0 69 4247.5 70 3544.0 71 2995.0 72 2098.0 73 1517.0 74 1284.0 75 1095.5 76 724.0 77 430.5 78 320.0 79 270.5 80 178.0 81 123.0 82 83.0 83 55.0 84 49.0 85 46.0 86 40.5 87 32.0 88 31.5 89 34.5 90 37.5 91 37.0 92 31.5 93 25.5 94 22.0 95 24.0 96 29.0 97 26.0 98 30.5 99 59.5 100 81.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010135669237858268 2 0.012173820117210202 3 0.01322043813633687 4 0.013991630360956521 5 0.011457713051491953 6 0.009033966059830196 7 0.014487396791069154 8 0.01608486639920986 9 0.016470462511519682 10-11 0.018811581764829336 12-13 0.015093333538984594 14-15 0.008125060937957035 16-17 0.006197080376407907 18-19 0.018398443073068812 20-21 0.024237469916617595 22-23 0.021593382289350222 24-25 0.01911455013878706 26-27 0.025201460197392157 28-29 0.008400486732464052 30-31 0.008648369947520369 32-33 0.009915328602252652 34-35 0.01184330916380178 36-37 0.011182287256984936 38-39 0.013137810397984766 40-41 0.010245839555661074 42-43 0.013771289725350905 44-45 0.0136611194075481 46-47 0.023824331224857068 48-49 0.024127299598814787 50-51 0.03756807837075727 52-53 0.041258784017151315 54-55 0.029360389694448135 56-57 0.021014988120885484 58-59 0.016002238660857756 60-61 0.0197480294661532 62-63 0.025173917617941455 64-65 0.025504428571349876 66-67 0.02784554782465953 68-69 0.043214307158151145 70-71 0.056682628509544336 72-73 0.08841168003675282 74-75 0.08846676519565423 76-77 0.09347951465568195 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1815371.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.88297457658402 #Duplication Level Percentage of deduplicated Percentage of total 1 70.95605009941221 36.104548932632134 2 13.882061785203776 14.127211937741846 3 5.931666469564364 9.054625025028585 4 3.0276756364001804 6.162285697323729 5 1.752466453149391 4.458535299095842 6 1.1073375534712118 3.3806777148583453 7 0.7376683117750688 2.6274330567801734 8 0.5150357586089185 2.0965241129063408 9 0.37301513852569645 1.7082107829255584 >10 1.603087004468283 13.75032055714828 >50 0.07840731649279252 2.7397039176764957 >100 0.03400305366835401 2.9431674519351128 >500 0.0013075022226313774 0.4000646754825833 >1k 1.0895851855261477E-4 0.1337799420647829 >5k 1.0895851855261477E-4 0.31291089640018016 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5644 0.31090063683952207 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2413 0.13292048842908696 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.5085158901403624E-5 0.0 2 0.0 0.0 0.0 4.40681271211229E-4 0.0 3 0.0 0.0 0.0 8.262773835210544E-4 0.0 4 0.0 0.0 0.0 0.0020932360382533376 0.0 5 0.0 0.0 0.0 0.004461897871013694 0.0 6 0.0 0.0 0.0 0.008097518358506334 0.0 7 0.0 0.0 0.0 0.016360292193716878 0.0 8 0.0 0.0 0.0 0.02693664270278637 0.0 9 0.0 0.0 0.0 0.04037742147472886 0.0 10 0.0 0.0 0.0 0.056186862079431694 0.0 11 0.0 0.0 0.0 0.07480564578810613 0.0 12 0.0 0.0 0.0 0.09821683832120266 0.0 13 0.0 0.0 0.0 0.12713654674443955 0.0 14 0.0 0.0 0.0 0.1580944060470284 0.0 15 0.0 0.0 0.0 0.19202686393029303 0.0 16 0.0 0.0 0.0 0.22535338506564223 0.0 17 0.0 0.0 0.0 0.26055280160363914 0.0 18 0.0 0.0 0.0 0.29564204782383324 0.0 19 0.0 0.0 0.0 0.3334304668301961 0.0 20 0.0 0.0 0.0 0.376947742362305 0.0 21 0.0 0.0 0.0 0.4261938744201598 0.0 22 0.0 0.0 0.0 0.4790205418066059 0.0 23 0.0 0.0 0.0 0.5317370388752491 0.0 24 0.0 0.0 0.0 0.5848391320562023 0.0 25 0.0 0.0 0.0 0.6386022471439722 0.0 26 0.0 0.0 0.0 0.6910433184181085 0.0 27 0.0 0.0 0.0 0.7450818593003854 0.0 28 0.0 0.0 0.0 0.8027009355112537 0.0 29 0.0 0.0 0.0 0.8615318852179527 0.0 30 0.0 0.0 0.0 0.9288459493954679 0.0 31 0.0 0.0 0.0 1.003927020978081 0.0 32 0.0 0.0 0.0 1.0800547105798208 0.0 33 0.0 0.0 0.0 1.1615256605949968 0.0 34 0.0 0.0 0.0 1.2442635692649051 0.0 35 0.0 0.0 0.0 1.3287091178607568 0.0 36 0.0 0.0 0.0 1.4198199706836785 0.0 37 0.0 0.0 0.0 1.5070748623835017 0.0 38 0.0 0.0 0.0 1.5971941823461981 0.0 39 0.0 0.0 0.0 1.6966228941632318 0.0 40 0.0 0.0 0.0 1.8012296109169972 0.0 41 0.0 0.0 0.0 1.917238955563353 0.0 42 0.0 0.0 0.0 2.047570441524074 0.0 43 0.0 0.0 0.0 2.188533363152766 0.0 44 0.0 0.0 0.0 2.3314793505019082 0.0 45 0.0 0.0 0.0 2.474755848804459 0.0 46 0.0 0.0 0.0 2.632850254851488 0.0 47 0.0 0.0 0.0 2.790283638991699 0.0 48 0.0 0.0 0.0 2.9506365365536853 0.0 49 0.0 0.0 0.0 3.115010650715474 0.0 50 0.0 0.0 0.0 3.293872161668331 0.0 51 0.0 0.0 0.0 3.486670217823244 0.0 52 0.0 0.0 0.0 3.68745562201886 0.0 53 0.0 0.0 0.0 3.9020674010987286 0.0 54 0.0 0.0 0.0 4.132268280147694 0.0 55 0.0 0.0 0.0 4.370511592396265 0.0 56 0.0 0.0 0.0 4.6223609388934825 0.0 57 0.0 0.0 0.0 4.877790820719291 0.0 58 0.0 0.0 0.0 5.145174182026704 0.0 59 0.0 0.0 0.0 5.412392287857413 0.0 60 0.0 0.0 0.0 5.688313848794544 5.5085158901403624E-5 61 0.0 0.0 0.0 5.993155118154911 5.5085158901403624E-5 62 0.0 0.0 0.0 6.299098090693307 5.5085158901403624E-5 63 0.0 0.0 0.0 6.637596392142433 5.5085158901403624E-5 64 0.0 0.0 0.0 6.980005739873557 5.5085158901403624E-5 65 0.0 0.0 0.0 7.342631340921497 5.5085158901403624E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCGTT 20 0.006574927 53.24688 60 CGGTTAC 245 0.0 23.178482 4 ATTCGCG 230 1.0913936E-11 21.606834 56 TTGCGTA 160 1.0378899E-6 19.965378 36 CGTTACA 90 0.0048640496 19.721066 27 CGCGGAA 280 9.094947E-12 19.016743 59 AATTCGC 285 1.0913936E-11 18.681055 55 CTCGCGC 310 1.8189894E-12 18.319489 36 TCGCGCT 295 1.8189894E-11 18.048794 37 CGATCGT 330 5.456968E-12 17.208267 1 CCTCGCG 355 1.8189894E-12 16.996662 35 TCGCGGA 320 6.366463E-11 16.638733 58 ATCGTTC 300 4.5474735E-10 16.5625 3 CGTAAGT 195 6.861428E-6 16.382301 39 GCGTAAG 195 6.861428E-6 16.382301 38 CAATTCG 285 4.098183E-9 16.193819 54 CGCTCCG 350 2.4738256E-10 15.213394 25 CGCGCTT 405 1.4733814E-10 14.022711 38 AACTCGA 355 4.9149094E-9 13.997637 13 TGACACG 360 5.980837E-9 13.803606 15 >>END_MODULE