FastQCFastQC Report
Thu 26 May 2016
SRR936123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815371
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46400.2555951373025128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36650.2018871073736443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23840.13132301882094624No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA7850.045.117348100-101
GGTATCA52100.039.403431
CGGTATC7900.039.18073100-101
CGTACAT8700.034.551617100-101
GCGTATC1650.034.27172100-101
CCGGTAT4000.033.48257100-101
CCGTATC2650.031.446941100-101
GCGTACA2750.031.385681100-101
TCGTATC3800.031.32872100-101
ACGTATC3450.031.056297100-101
CCGTACA5250.030.612637100-101
GTATCAA96600.030.1836411
ATACACG6550.029.53020996-97
CACATCG6650.029.08614596-97
CCTGTCG7400.028.9531196-97
CGAGCCG3300.028.85562596-97
ACGGTAT3000.028.770208100-101
CCCGTAC2300.028.4674998-99
GGGTATC7450.028.364056100-101
TCGGTAT2800.027.636406100-101
Produced by FastQC (version 0.11.5)